Putative tubulin-tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase, putative

Species:

Leishmania mexicana

UniProt:

E9AUL7_LEIMU

TriTrypDb:

LmxM.20.0790

Length:

720

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 23
NetGPI	no	yes: 0, no: 23

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	6
GO:0042995	cell projection	2	6
GO:0043226	organelle	2	6
GO:0043227	membrane-bounded organelle	3	6
GO:0110165	cellular anatomical entity	1	6
GO:0120025	plasma membrane bounded cell projection	3	6
GO:0005874	microtubule	6	4
GO:0099080	supramolecular complex	2	4
GO:0099081	supramolecular polymer	3	4
GO:0099512	supramolecular fiber	4	4
GO:0099513	polymeric cytoskeletal fiber	5	4
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

E9AUL7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUL7

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	24
GO:0008152	metabolic process	1	24
GO:0019538	protein metabolic process	3	24
GO:0036211	protein modification process	4	24
GO:0043170	macromolecule metabolic process	3	24
GO:0043412	macromolecule modification	4	24
GO:0044238	primary metabolic process	2	24
GO:0071704	organic substance metabolic process	2	24
GO:1901564	organonitrogen compound metabolic process	3	24
GO:0000226	microtubule cytoskeleton organization	3	3
GO:0006996	organelle organization	4	3
GO:0007010	cytoskeleton organization	5	3
GO:0007017	microtubule-based process	2	3
GO:0009987	cellular process	1	3
GO:0016043	cellular component organization	3	3
GO:0018095	protein polyglutamylation	7	6
GO:0018193	peptidyl-amino acid modification	5	6
GO:0018200	peptidyl-glutamic acid modification	6	6
GO:0071840	cellular component organization or biogenesis	2	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	21
GO:0016874	ligase activity	2	21
GO:0000166	nucleotide binding	3	9
GO:0005488	binding	1	10
GO:0005524	ATP binding	5	9
GO:0017076	purine nucleotide binding	4	9
GO:0030554	adenyl nucleotide binding	5	9
GO:0032553	ribonucleotide binding	3	9
GO:0032555	purine ribonucleotide binding	4	9
GO:0032559	adenyl ribonucleotide binding	5	9
GO:0035639	purine ribonucleoside triphosphate binding	4	9
GO:0036094	small molecule binding	2	9
GO:0043167	ion binding	2	9
GO:0043168	anion binding	3	9
GO:0043169	cation binding	3	5
GO:0046872	metal ion binding	4	5
GO:0097159	organic cyclic compound binding	2	9
GO:0097367	carbohydrate derivative binding	2	9
GO:1901265	nucleoside phosphate binding	3	9
GO:1901363	heterocyclic compound binding	2	9
GO:0004835	tubulin-tyrosine ligase activity	3	6
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	7
GO:0016881	acid-amino acid ligase activity	4	7
GO:0140096	catalytic activity, acting on a protein	2	7
GO:0005515	protein binding	2	3
GO:0008092	cytoskeletal protein binding	3	3
GO:0015631	tubulin binding	4	3
GO:0070739	protein-glutamic acid ligase activity	3	3
GO:0070740	tubulin-glutamic acid ligase activity	4	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	354	358	PF00656	0.371
CLV_C14_Caspase3-7	695	699	PF00656	0.514
CLV_C14_Caspase3-7	706	710	PF00656	0.309
CLV_NRD_NRD_1	109	111	PF00675	0.633
CLV_NRD_NRD_1	184	186	PF00675	0.340
CLV_NRD_NRD_1	197	199	PF00675	0.358
CLV_NRD_NRD_1	207	209	PF00675	0.361
CLV_NRD_NRD_1	338	340	PF00675	0.315
CLV_NRD_NRD_1	36	38	PF00675	0.442
CLV_NRD_NRD_1	382	384	PF00675	0.317
CLV_NRD_NRD_1	670	672	PF00675	0.386
CLV_PCSK_KEX2_1	109	111	PF00082	0.636
CLV_PCSK_KEX2_1	166	168	PF00082	0.403
CLV_PCSK_KEX2_1	207	209	PF00082	0.328
CLV_PCSK_KEX2_1	337	339	PF00082	0.190
CLV_PCSK_KEX2_1	35	37	PF00082	0.470
CLV_PCSK_KEX2_1	670	672	PF00082	0.393
CLV_PCSK_PC1ET2_1	166	168	PF00082	0.317
CLV_PCSK_PC1ET2_1	35	37	PF00082	0.614
CLV_PCSK_SKI1_1	152	156	PF00082	0.603
CLV_PCSK_SKI1_1	166	170	PF00082	0.461
CLV_PCSK_SKI1_1	339	343	PF00082	0.208
CLV_PCSK_SKI1_1	498	502	PF00082	0.243
CLV_PCSK_SKI1_1	509	513	PF00082	0.232
CLV_PCSK_SKI1_1	583	587	PF00082	0.339
CLV_Separin_Metazoa	495	499	PF03568	0.539
DEG_APCC_DBOX_1	404	412	PF00400	0.550
DEG_APCC_DBOX_1	582	590	PF00400	0.526
DEG_SPOP_SBC_1	9	13	PF00917	0.576
DOC_CYCLIN_yCln2_LP_2	620	623	PF00134	0.421
DOC_MAPK_gen_1	198	206	PF00069	0.462
DOC_MAPK_gen_1	35	43	PF00069	0.410
DOC_MAPK_gen_1	383	391	PF00069	0.535
DOC_MAPK_gen_1	687	694	PF00069	0.600
DOC_MAPK_JIP1_4	583	589	PF00069	0.526
DOC_MAPK_MEF2A_6	35	43	PF00069	0.446
DOC_MAPK_MEF2A_6	383	391	PF00069	0.466
DOC_MAPK_MEF2A_6	492	501	PF00069	0.451
DOC_MAPK_MEF2A_6	583	591	PF00069	0.467
DOC_MAPK_MEF2A_6	707	716	PF00069	0.255
DOC_MAPK_NFAT4_5	384	392	PF00069	0.480
DOC_PP1_RVXF_1	496	502	PF00149	0.456
DOC_PP2B_LxvP_1	620	623	PF13499	0.421
DOC_PP4_MxPP_1	621	624	PF00568	0.499
DOC_USP7_MATH_1	238	242	PF00917	0.526
DOC_USP7_MATH_1	263	267	PF00917	0.537
DOC_USP7_MATH_1	421	425	PF00917	0.591
DOC_USP7_MATH_1	431	435	PF00917	0.431
DOC_USP7_MATH_1	461	465	PF00917	0.535
DOC_USP7_MATH_1	527	531	PF00917	0.530
DOC_USP7_MATH_1	532	536	PF00917	0.532
DOC_USP7_MATH_1	573	577	PF00917	0.551
DOC_USP7_MATH_1	703	707	PF00917	0.490
DOC_USP7_MATH_1	97	101	PF00917	0.701
DOC_WW_Pin1_4	129	134	PF00397	0.357
DOC_WW_Pin1_4	240	245	PF00397	0.483
DOC_WW_Pin1_4	29	34	PF00397	0.442
DOC_WW_Pin1_4	328	333	PF00397	0.610
DOC_WW_Pin1_4	411	416	PF00397	0.442
DOC_WW_Pin1_4	447	452	PF00397	0.494
DOC_WW_Pin1_4	502	507	PF00397	0.506
DOC_WW_Pin1_4	544	549	PF00397	0.391
DOC_WW_Pin1_4	569	574	PF00397	0.462
DOC_WW_Pin1_4	699	704	PF00397	0.533
LIG_14-3-3_CanoR_1	122	126	PF00244	0.481
LIG_14-3-3_CanoR_1	337	342	PF00244	0.406
LIG_14-3-3_CanoR_1	410	415	PF00244	0.442
LIG_14-3-3_CanoR_1	418	427	PF00244	0.442
LIG_14-3-3_CanoR_1	513	520	PF00244	0.581
LIG_14-3-3_CanoR_1	652	659	PF00244	0.511
LIG_Actin_WH2_2	497	515	PF00022	0.445
LIG_APCC_ABBA_1	401	406	PF00400	0.469
LIG_APCC_ABBAyCdc20_2	400	406	PF00400	0.431
LIG_BRCT_BRCA1_1	546	550	PF00533	0.526
LIG_EH_1	375	379	PF12763	0.526
LIG_EH1_1	552	560	PF00400	0.442
LIG_FHA_1	133	139	PF00498	0.434
LIG_FHA_1	157	163	PF00498	0.479
LIG_FHA_1	191	197	PF00498	0.460
LIG_FHA_1	299	305	PF00498	0.463
LIG_FHA_1	415	421	PF00498	0.541
LIG_FHA_1	503	509	PF00498	0.534
LIG_FHA_1	674	680	PF00498	0.451
LIG_FHA_2	12	18	PF00498	0.639
LIG_FHA_2	312	318	PF00498	0.408
LIG_FHA_2	338	344	PF00498	0.583
LIG_FHA_2	352	358	PF00498	0.444
LIG_FHA_2	392	398	PF00498	0.442
LIG_FHA_2	646	652	PF00498	0.332
LIG_FHA_2	663	669	PF00498	0.391
LIG_LIR_Apic_2	245	250	PF02991	0.467
LIG_LIR_Apic_2	481	487	PF02991	0.385
LIG_LIR_Gen_1	132	141	PF02991	0.327
LIG_LIR_Gen_1	547	558	PF02991	0.549
LIG_LIR_Gen_1	596	606	PF02991	0.442
LIG_LIR_Gen_1	673	680	PF02991	0.438
LIG_LIR_Nem_3	132	137	PF02991	0.420
LIG_LIR_Nem_3	394	399	PF02991	0.472
LIG_LIR_Nem_3	40	46	PF02991	0.502
LIG_LIR_Nem_3	546	552	PF02991	0.489
LIG_LIR_Nem_3	596	602	PF02991	0.451
LIG_LIR_Nem_3	673	678	PF02991	0.403
LIG_LIR_Nem_3	686	691	PF02991	0.389
LIG_MLH1_MIPbox_1	546	550	PF16413	0.421
LIG_NRBOX	201	207	PF00104	0.428
LIG_PCNA_PIPBox_1	592	601	PF02747	0.459
LIG_PCNA_yPIPBox_3	365	374	PF02747	0.401
LIG_Pex14_2	549	553	PF04695	0.494
LIG_SH2_CRK	120	124	PF00017	0.393
LIG_SH2_CRK	184	188	PF00017	0.529
LIG_SH2_CRK	396	400	PF00017	0.520
LIG_SH2_CRK	46	50	PF00017	0.471
LIG_SH2_CRK	688	692	PF00017	0.429
LIG_SH2_NCK_1	46	50	PF00017	0.471
LIG_SH2_SRC	396	399	PF00017	0.421
LIG_SH2_STAP1	416	420	PF00017	0.410
LIG_SH2_STAT3	368	371	PF00017	0.501
LIG_SH2_STAT5	125	128	PF00017	0.470
LIG_SH2_STAT5	28	31	PF00017	0.503
LIG_SH2_STAT5	368	371	PF00017	0.491
LIG_SH2_STAT5	373	376	PF00017	0.475
LIG_SH2_STAT5	390	393	PF00017	0.458
LIG_SH2_STAT5	402	405	PF00017	0.466
LIG_SH2_STAT5	416	419	PF00017	0.448
LIG_SH2_STAT5	478	481	PF00017	0.525
LIG_SH3_3	113	119	PF00018	0.427
LIG_SH3_3	27	33	PF00018	0.481
LIG_SH3_3	3	9	PF00018	0.717
LIG_SH3_4	276	283	PF00018	0.408
LIG_SUMO_SIM_anti_2	556	566	PF11976	0.526
LIG_SUMO_SIM_anti_2	613	619	PF11976	0.431
LIG_SUMO_SIM_par_1	556	568	PF11976	0.526
LIG_SUMO_SIM_par_1	583	588	PF11976	0.472
LIG_TRAF2_1	315	318	PF00917	0.408
LIG_TRAF2_1	58	61	PF00917	0.745
LIG_TRAF2_1	633	636	PF00917	0.459
LIG_TRAF2_1	664	667	PF00917	0.342
LIG_TYR_ITSM	392	399	PF00017	0.421
MOD_CDC14_SPxK_1	32	35	PF00782	0.464
MOD_CDK_SPxK_1	29	35	PF00069	0.473
MOD_CDK_SPxK_1	701	707	PF00069	0.578
MOD_CDK_SPxxK_3	29	36	PF00069	0.504
MOD_CDK_SPxxK_3	411	418	PF00069	0.408
MOD_CDK_SPxxK_3	502	509	PF00069	0.526
MOD_CK1_1	10	16	PF00069	0.706
MOD_CK1_1	121	127	PF00069	0.559
MOD_CK1_1	328	334	PF00069	0.503
MOD_CK1_1	414	420	PF00069	0.570
MOD_CK1_1	471	477	PF00069	0.457
MOD_CK1_1	522	528	PF00069	0.461
MOD_CK1_1	530	536	PF00069	0.497
MOD_CK1_1	539	545	PF00069	0.593
MOD_CK1_1	564	570	PF00069	0.412
MOD_CK1_1	578	584	PF00069	0.492
MOD_CK1_1	594	600	PF00069	0.454
MOD_CK1_1	701	707	PF00069	0.543
MOD_CK1_1	92	98	PF00069	0.732
MOD_CK2_1	12	18	PF00069	0.673
MOD_CK2_1	311	317	PF00069	0.471
MOD_CK2_1	337	343	PF00069	0.408
MOD_CK2_1	470	476	PF00069	0.500
MOD_CK2_1	55	61	PF00069	0.720
MOD_CK2_1	651	657	PF00069	0.477
MOD_CK2_1	662	668	PF00069	0.368
MOD_Cter_Amidation	196	199	PF01082	0.231
MOD_Cter_Amidation	381	384	PF01082	0.335
MOD_GlcNHglycan	14	17	PF01048	0.665
MOD_GlcNHglycan	149	152	PF01048	0.651
MOD_GlcNHglycan	215	218	PF01048	0.338
MOD_GlcNHglycan	220	223	PF01048	0.324
MOD_GlcNHglycan	265	268	PF01048	0.390
MOD_GlcNHglycan	322	325	PF01048	0.255
MOD_GlcNHglycan	333	336	PF01048	0.195
MOD_GlcNHglycan	423	426	PF01048	0.330
MOD_GlcNHglycan	463	466	PF01048	0.261
MOD_GlcNHglycan	521	524	PF01048	0.311
MOD_GlcNHglycan	534	537	PF01048	0.369
MOD_GlcNHglycan	57	60	PF01048	0.581
MOD_GlcNHglycan	575	578	PF01048	0.383
MOD_GlcNHglycan	629	632	PF01048	0.618
MOD_GlcNHglycan	683	686	PF01048	0.525
MOD_GlcNHglycan	91	94	PF01048	0.771
MOD_GSK3_1	213	220	PF00069	0.515
MOD_GSK3_1	296	303	PF00069	0.533
MOD_GSK3_1	327	334	PF00069	0.572
MOD_GSK3_1	337	344	PF00069	0.530
MOD_GSK3_1	410	417	PF00069	0.488
MOD_GSK3_1	421	428	PF00069	0.480
MOD_GSK3_1	461	468	PF00069	0.507
MOD_GSK3_1	527	534	PF00069	0.545
MOD_GSK3_1	535	542	PF00069	0.559
MOD_GSK3_1	561	568	PF00069	0.516
MOD_GSK3_1	569	576	PF00069	0.508
MOD_GSK3_1	593	600	PF00069	0.473
MOD_GSK3_1	622	629	PF00069	0.500
MOD_GSK3_1	697	704	PF00069	0.625
MOD_GSK3_1	7	14	PF00069	0.649
MOD_N-GLC_1	312	317	PF02516	0.267
MOD_N-GLC_1	441	446	PF02516	0.257
MOD_N-GLC_1	447	452	PF02516	0.272
MOD_N-GLC_1	543	548	PF02516	0.201
MOD_N-GLC_1	55	60	PF02516	0.510
MOD_N-GLC_1	645	650	PF02516	0.335
MOD_N-GLC_1	673	678	PF02516	0.349
MOD_N-GLC_2	660	662	PF02516	0.320
MOD_NEK2_1	127	132	PF00069	0.416
MOD_NEK2_1	137	142	PF00069	0.428
MOD_NEK2_1	156	161	PF00069	0.407
MOD_NEK2_1	22	27	PF00069	0.457
MOD_NEK2_1	300	305	PF00069	0.477
MOD_NEK2_1	327	332	PF00069	0.447
MOD_NEK2_1	341	346	PF00069	0.539
MOD_NEK2_1	512	517	PF00069	0.535
MOD_NEK2_1	89	94	PF00069	0.523
MOD_PIKK_1	104	110	PF00454	0.535
MOD_PIKK_1	363	369	PF00454	0.398
MOD_PKA_1	337	343	PF00069	0.494
MOD_PKA_2	121	127	PF00069	0.484
MOD_PKA_2	147	153	PF00069	0.548
MOD_PKA_2	337	343	PF00069	0.410
MOD_PKA_2	512	518	PF00069	0.586
MOD_PKA_2	651	657	PF00069	0.522
MOD_PKA_2	681	687	PF00069	0.548
MOD_PKB_1	337	345	PF00069	0.391
MOD_PKB_1	517	525	PF00069	0.371
MOD_Plk_1	441	447	PF00069	0.446
MOD_Plk_1	564	570	PF00069	0.521
MOD_Plk_1	645	651	PF00069	0.336
MOD_Plk_1	673	679	PF00069	0.488
MOD_Plk_2-3	565	571	PF00069	0.371
MOD_Plk_2-3	651	657	PF00069	0.338
MOD_Plk_4	1	7	PF00069	0.667
MOD_Plk_4	121	127	PF00069	0.499
MOD_Plk_4	158	164	PF00069	0.443
MOD_Plk_4	168	174	PF00069	0.493
MOD_Plk_4	243	249	PF00069	0.499
MOD_Plk_4	351	357	PF00069	0.561
MOD_Plk_4	597	603	PF00069	0.460
MOD_Plk_4	613	619	PF00069	0.425
MOD_Plk_4	64	70	PF00069	0.536
MOD_Plk_4	645	651	PF00069	0.331
MOD_Plk_4	710	716	PF00069	0.418
MOD_Plk_4	97	103	PF00069	0.532
MOD_ProDKin_1	129	135	PF00069	0.352
MOD_ProDKin_1	240	246	PF00069	0.483
MOD_ProDKin_1	29	35	PF00069	0.445
MOD_ProDKin_1	328	334	PF00069	0.610
MOD_ProDKin_1	411	417	PF00069	0.442
MOD_ProDKin_1	447	453	PF00069	0.494
MOD_ProDKin_1	502	508	PF00069	0.506
MOD_ProDKin_1	544	550	PF00069	0.391
MOD_ProDKin_1	569	575	PF00069	0.462
MOD_ProDKin_1	699	705	PF00069	0.518
MOD_SUMO_rev_2	246	255	PF00179	0.371
MOD_SUMO_rev_2	432	438	PF00179	0.598
MOD_SUMO_rev_2	464	471	PF00179	0.426
TRG_DiLeu_BaEn_1	495	500	PF01217	0.458
TRG_DiLeu_BaLyEn_6	164	169	PF01217	0.278
TRG_DiLeu_BaLyEn_6	674	679	PF01217	0.468
TRG_ENDOCYTIC_2	120	123	PF00928	0.421
TRG_ENDOCYTIC_2	184	187	PF00928	0.499
TRG_ENDOCYTIC_2	396	399	PF00928	0.486
TRG_ENDOCYTIC_2	599	602	PF00928	0.447
TRG_ENDOCYTIC_2	688	691	PF00928	0.344
TRG_ER_diArg_1	109	111	PF00400	0.612
TRG_ER_diArg_1	206	208	PF00400	0.547
TRG_ER_diArg_1	336	339	PF00400	0.391
TRG_ER_diArg_1	517	520	PF00400	0.422
TRG_NES_CRM1_1	495	507	PF08389	0.455
TRG_NES_CRM1_1	551	563	PF08389	0.421
TRG_NLS_MonoExtC_3	34	39	PF00514	0.353
TRG_NLS_MonoExtN_4	33	39	PF00514	0.358
TRG_Pf-PMV_PEXEL_1	258	262	PF00026	0.257
TRG_Pf-PMV_PEXEL_1	339	343	PF00026	0.208
TRG_Pf-PMV_PEXEL_1	689	693	PF00026	0.579

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7G7	Leptomonas seymouri	43%	88%
A0A0N1I1Q6	Leptomonas seymouri	27%	100%
A0A0S4ISF0	Bodo saltans	26%	100%
A0A0S4KQK8	Bodo saltans	29%	100%
A0A1X0NXX8	Trypanosomatidae	31%	99%
A0A3Q8IDL7	Leishmania donovani	89%	99%
A0A3Q8IEC1	Leishmania donovani	28%	100%
A0A422NPL9	Trypanosoma rangeli	33%	100%
A4H8G3	Leishmania braziliensis	24%	98%
A4HG29	Leishmania braziliensis	24%	100%
A4HWT7	Leishmania infantum	25%	88%
A4HYN9	Leishmania infantum	89%	99%
A4I5Q6	Leishmania infantum	28%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
C9ZR25	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
E9AID0	Leishmania braziliensis	76%	100%
E9AM52	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AQJ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	88%
E9B101	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
Q4Q765	Leishmania major	26%	100%
Q4QCW7	Leishmania major	90%	100%
Q4QEY2	Leishmania major	24%	89%