ZZ-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase KCMF1, putative

Species:

Leishmania mexicana

UniProt:

E9AUH0_LEIMU

TriTrypDb:

LmxM.20.0290

Length:

799

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005622	intracellular anatomical structure	2	1
GO:0005737	cytoplasm	2	1
GO:0005773	vacuole	5	1
GO:0005776	autophagosome	6	1
GO:0016234	inclusion body	3	1
GO:0016235	aggresome	4	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043233	organelle lumen	3	1
GO:0044753	amphisome	7	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AUH0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUH0

Function

Biological processes
Term	Name	Level	Count
GO:0000422	autophagy of mitochondrion	4	1
GO:0000423	mitophagy	5	1
GO:0006914	autophagy	3	1
GO:0006996	organelle organization	4	1
GO:0007005	mitochondrion organization	5	1
GO:0007032	endosome organization	6	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0016050	vesicle organization	5	1
GO:0016236	macroautophagy	4	1
GO:0022411	cellular component disassembly	4	1
GO:0035973	aggrephagy	6	1
GO:0044237	cellular metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0061726	mitochondrion disassembly	6	1
GO:0061912	selective autophagy	5	1
GO:0061919	process utilizing autophagic mechanism	2	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1903008	organelle disassembly	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0008270	zinc ion binding	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0005080	protein kinase C binding	6	1
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0019900	kinase binding	4	1
GO:0019901	protein kinase binding	5	1
GO:0031593	polyubiquitin modification-dependent protein binding	4	1
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	5	1
GO:0140030	modification-dependent protein binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	229	233	PF00656	0.524
CLV_C14_Caspase3-7	472	476	PF00656	0.514
CLV_NRD_NRD_1	460	462	PF00675	0.652
CLV_NRD_NRD_1	556	558	PF00675	0.555
CLV_NRD_NRD_1	71	73	PF00675	0.462
CLV_PCSK_FUR_1	458	462	PF00082	0.656
CLV_PCSK_KEX2_1	341	343	PF00082	0.362
CLV_PCSK_KEX2_1	37	39	PF00082	0.431
CLV_PCSK_KEX2_1	458	460	PF00082	0.770
CLV_PCSK_KEX2_1	556	558	PF00082	0.462
CLV_PCSK_KEX2_1	71	73	PF00082	0.423
CLV_PCSK_KEX2_1	721	723	PF00082	0.494
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.362
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.431
CLV_PCSK_PC1ET2_1	721	723	PF00082	0.551
CLV_PCSK_SKI1_1	281	285	PF00082	0.439
CLV_PCSK_SKI1_1	290	294	PF00082	0.499
CLV_PCSK_SKI1_1	495	499	PF00082	0.280
CLV_PCSK_SKI1_1	71	75	PF00082	0.442
CLV_PCSK_SKI1_1	84	88	PF00082	0.294
DEG_APCC_DBOX_1	250	258	PF00400	0.472
DEG_SCF_FBW7_1	573	579	PF00400	0.389
DEG_SPOP_SBC_1	531	535	PF00917	0.262
DOC_CDC14_PxL_1	769	777	PF14671	0.264
DOC_CKS1_1	123	128	PF01111	0.502
DOC_CKS1_1	573	578	PF01111	0.517
DOC_CKS1_1	583	588	PF01111	0.463
DOC_CYCLIN_RxL_1	278	287	PF00134	0.490
DOC_CYCLIN_yClb1_LxF_4	204	210	PF00134	0.466
DOC_CYCLIN_yCln2_LP_2	120	126	PF00134	0.433
DOC_CYCLIN_yCln2_LP_2	646	652	PF00134	0.348
DOC_MAPK_gen_1	341	348	PF00069	0.344
DOC_MAPK_gen_1	459	471	PF00069	0.600
DOC_MAPK_MEF2A_6	341	350	PF00069	0.317
DOC_MAPK_MEF2A_6	464	471	PF00069	0.594
DOC_PP2B_LxvP_1	646	649	PF13499	0.467
DOC_PP2B_LxvP_1	770	773	PF13499	0.469
DOC_PP4_FxxP_1	783	786	PF00568	0.512
DOC_USP7_MATH_1	310	314	PF00917	0.244
DOC_USP7_MATH_1	519	523	PF00917	0.608
DOC_USP7_MATH_1	532	536	PF00917	0.225
DOC_USP7_MATH_1	576	580	PF00917	0.537
DOC_USP7_MATH_1	678	682	PF00917	0.481
DOC_USP7_MATH_1	759	763	PF00917	0.591
DOC_USP7_UBL2_3	337	341	PF12436	0.552
DOC_WW_Pin1_4	122	127	PF00397	0.436
DOC_WW_Pin1_4	370	375	PF00397	0.397
DOC_WW_Pin1_4	451	456	PF00397	0.624
DOC_WW_Pin1_4	5	10	PF00397	0.584
DOC_WW_Pin1_4	572	577	PF00397	0.456
DOC_WW_Pin1_4	582	587	PF00397	0.441
DOC_WW_Pin1_4	744	749	PF00397	0.629
DOC_WW_Pin1_4	751	756	PF00397	0.614
LIG_14-3-3_CanoR_1	251	255	PF00244	0.360
LIG_14-3-3_CanoR_1	436	444	PF00244	0.521
LIG_14-3-3_CanoR_1	517	525	PF00244	0.429
LIG_14-3-3_CanoR_1	785	795	PF00244	0.290
LIG_14-3-3_CanoR_1	95	100	PF00244	0.363
LIG_Actin_WH2_2	276	292	PF00022	0.332
LIG_Actin_WH2_2	29	47	PF00022	0.432
LIG_AP2alpha_2	309	311	PF02296	0.358
LIG_AP2alpha_2	523	525	PF02296	0.496
LIG_BIR_II_1	1	5	PF00653	0.603
LIG_BIR_III_2	406	410	PF00653	0.327
LIG_Clathr_ClatBox_1	347	351	PF01394	0.311
LIG_Clathr_ClatBox_1	395	399	PF01394	0.424
LIG_deltaCOP1_diTrp_1	277	284	PF00928	0.481
LIG_deltaCOP1_diTrp_1	309	314	PF00928	0.365
LIG_FHA_1	105	111	PF00498	0.488
LIG_FHA_1	123	129	PF00498	0.264
LIG_FHA_1	328	334	PF00498	0.562
LIG_FHA_1	421	427	PF00498	0.660
LIG_FHA_1	439	445	PF00498	0.606
LIG_FHA_1	466	472	PF00498	0.689
LIG_FHA_1	505	511	PF00498	0.376
LIG_FHA_1	573	579	PF00498	0.502
LIG_FHA_1	592	598	PF00498	0.357
LIG_FHA_1	622	628	PF00498	0.284
LIG_FHA_1	68	74	PF00498	0.434
LIG_FHA_1	752	758	PF00498	0.685
LIG_FHA_1	787	793	PF00498	0.416
LIG_FHA_2	184	190	PF00498	0.771
LIG_FHA_2	244	250	PF00498	0.487
LIG_FHA_2	542	548	PF00498	0.443
LIG_IBAR_NPY_1	518	520	PF08397	0.495
LIG_LIR_Apic_2	782	786	PF02991	0.526
LIG_LIR_Gen_1	213	222	PF02991	0.602
LIG_LIR_Gen_1	299	310	PF02991	0.507
LIG_LIR_Gen_1	30	39	PF02991	0.389
LIG_LIR_Gen_1	475	483	PF02991	0.488
LIG_LIR_Gen_1	793	799	PF02991	0.266
LIG_LIR_Nem_3	19	23	PF02991	0.442
LIG_LIR_Nem_3	213	217	PF02991	0.451
LIG_LIR_Nem_3	299	305	PF02991	0.521
LIG_LIR_Nem_3	30	36	PF02991	0.349
LIG_LIR_Nem_3	309	314	PF02991	0.419
LIG_LIR_Nem_3	475	481	PF02991	0.457
LIG_LIR_Nem_3	522	528	PF02991	0.508
LIG_LIR_Nem_3	793	798	PF02991	0.260
LIG_PCNA_PIPBox_1	389	398	PF02747	0.483
LIG_PCNA_yPIPBox_3	389	397	PF02747	0.353
LIG_Pex14_1	136	140	PF04695	0.372
LIG_Pex14_1	268	272	PF04695	0.432
LIG_SH2_CRK	302	306	PF00017	0.490
LIG_SH2_CRK	33	37	PF00017	0.243
LIG_SH2_STAP1	210	214	PF00017	0.507
LIG_SH2_STAP1	302	306	PF00017	0.490
LIG_SH2_STAP1	719	723	PF00017	0.585
LIG_SH2_STAT5	140	143	PF00017	0.528
LIG_SH2_STAT5	514	517	PF00017	0.581
LIG_SH2_STAT5	520	523	PF00017	0.544
LIG_SH2_STAT5	542	545	PF00017	0.393
LIG_SH2_STAT5	688	691	PF00017	0.294
LIG_SH2_STAT5	81	84	PF00017	0.444
LIG_SH3_2	323	328	PF14604	0.533
LIG_SH3_3	120	126	PF00018	0.452
LIG_SH3_3	320	326	PF00018	0.481
LIG_SH3_3	570	576	PF00018	0.432
LIG_SH3_3	649	655	PF00018	0.489
LIG_SH3_3	738	744	PF00018	0.688
LIG_SH3_3	747	753	PF00018	0.583
LIG_SH3_3	781	787	PF00018	0.421
LIG_SH3_4	337	344	PF00018	0.323
LIG_SUMO_SIM_anti_2	375	380	PF11976	0.403
LIG_SUMO_SIM_anti_2	566	572	PF11976	0.420
LIG_SUMO_SIM_par_1	431	438	PF11976	0.420
LIG_SUMO_SIM_par_1	467	473	PF11976	0.622
LIG_TRAF2_1	186	189	PF00917	0.571
LIG_TRAF2_1	8	11	PF00917	0.565
LIG_TYR_ITIM	74	79	PF00017	0.408
LIG_UBA3_1	335	341	PF00899	0.544
LIG_Vh1_VBS_1	617	635	PF01044	0.271
MOD_CDC14_SPxK_1	749	752	PF00782	0.571
MOD_CDK_SPK_2	451	456	PF00069	0.643
MOD_CDK_SPxK_1	746	752	PF00069	0.571
MOD_CDK_SPxxK_3	451	458	PF00069	0.717
MOD_CDK_SPxxK_3	582	589	PF00069	0.558
MOD_CK1_1	127	133	PF00069	0.493
MOD_CK1_1	169	175	PF00069	0.557
MOD_CK1_1	2	8	PF00069	0.630
MOD_CK1_1	413	419	PF00069	0.735
MOD_CK1_1	420	426	PF00069	0.610
MOD_CK1_1	438	444	PF00069	0.487
MOD_CK1_1	566	572	PF00069	0.389
MOD_CK1_1	621	627	PF00069	0.596
MOD_CK1_1	717	723	PF00069	0.457
MOD_CK1_1	725	731	PF00069	0.583
MOD_CK1_1	746	752	PF00069	0.686
MOD_CK1_1	779	785	PF00069	0.678
MOD_CK2_1	171	177	PF00069	0.676
MOD_CK2_1	183	189	PF00069	0.759
MOD_CK2_1	243	249	PF00069	0.433
MOD_CK2_1	5	11	PF00069	0.575
MOD_CK2_1	566	572	PF00069	0.551
MOD_GlcNHglycan	168	171	PF01048	0.567
MOD_GlcNHglycan	200	203	PF01048	0.651
MOD_GlcNHglycan	227	231	PF01048	0.576
MOD_GlcNHglycan	298	301	PF01048	0.529
MOD_GlcNHglycan	39	42	PF01048	0.443
MOD_GlcNHglycan	623	627	PF01048	0.362
MOD_GlcNHglycan	729	732	PF01048	0.431
MOD_GlcNHglycan	778	781	PF01048	0.580
MOD_GSK3_1	1	8	PF00069	0.699
MOD_GSK3_1	169	176	PF00069	0.587
MOD_GSK3_1	183	190	PF00069	0.757
MOD_GSK3_1	243	250	PF00069	0.442
MOD_GSK3_1	387	394	PF00069	0.428
MOD_GSK3_1	412	419	PF00069	0.672
MOD_GSK3_1	434	441	PF00069	0.550
MOD_GSK3_1	465	472	PF00069	0.684
MOD_GSK3_1	491	498	PF00069	0.298
MOD_GSK3_1	572	579	PF00069	0.537
MOD_GSK3_1	591	598	PF00069	0.422
MOD_GSK3_1	618	625	PF00069	0.441
MOD_GSK3_1	723	730	PF00069	0.463
MOD_GSK3_1	742	749	PF00069	0.665
MOD_GSK3_1	759	766	PF00069	0.361
MOD_GSK3_1	786	793	PF00069	0.373
MOD_N-GLC_1	284	289	PF02516	0.401
MOD_N-GLC_1	426	431	PF02516	0.689
MOD_N-GLC_1	566	571	PF02516	0.437
MOD_N-GLC_1	790	795	PF02516	0.250
MOD_N-GLC_2	690	692	PF02516	0.294
MOD_NEK2_1	198	203	PF00069	0.676
MOD_NEK2_1	243	248	PF00069	0.460
MOD_NEK2_1	284	289	PF00069	0.424
MOD_NEK2_1	293	298	PF00069	0.481
MOD_NEK2_1	504	509	PF00069	0.447
MOD_NEK2_1	577	582	PF00069	0.432
MOD_NEK2_1	67	72	PF00069	0.334
MOD_NEK2_1	711	716	PF00069	0.362
MOD_NEK2_2	327	332	PF00069	0.467
MOD_NEK2_2	532	537	PF00069	0.251
MOD_PIKK_1	495	501	PF00454	0.537
MOD_PK_1	95	101	PF00069	0.381
MOD_PKA_1	37	43	PF00069	0.447
MOD_PKA_2	166	172	PF00069	0.608
MOD_PKA_2	250	256	PF00069	0.493
MOD_PKA_2	37	43	PF00069	0.447
MOD_PKA_2	413	419	PF00069	0.586
MOD_PKA_2	420	426	PF00069	0.604
MOD_PKA_2	435	441	PF00069	0.601
MOD_PKA_2	491	497	PF00069	0.464
MOD_PKA_2	696	702	PF00069	0.471
MOD_Plk_1	284	290	PF00069	0.512
MOD_Plk_1	566	572	PF00069	0.500
MOD_Plk_1	622	628	PF00069	0.410
MOD_Plk_2-3	177	183	PF00069	0.443
MOD_Plk_4	260	266	PF00069	0.324
MOD_Plk_4	377	383	PF00069	0.392
MOD_Plk_4	391	397	PF00069	0.409
MOD_Plk_4	566	572	PF00069	0.498
MOD_Plk_4	714	720	PF00069	0.233
MOD_Plk_4	779	785	PF00069	0.583
MOD_ProDKin_1	122	128	PF00069	0.444
MOD_ProDKin_1	370	376	PF00069	0.388
MOD_ProDKin_1	451	457	PF00069	0.631
MOD_ProDKin_1	5	11	PF00069	0.581
MOD_ProDKin_1	572	578	PF00069	0.460
MOD_ProDKin_1	582	588	PF00069	0.441
MOD_ProDKin_1	744	750	PF00069	0.626
MOD_ProDKin_1	751	757	PF00069	0.613
TRG_DiLeu_BaEn_1	213	218	PF01217	0.551
TRG_DiLeu_BaEn_1	53	58	PF01217	0.367
TRG_DiLeu_BaEn_2	398	404	PF01217	0.428
TRG_DiLeu_BaLyEn_6	655	660	PF01217	0.487
TRG_DiLeu_BaLyEn_6	69	74	PF01217	0.423
TRG_ENDOCYTIC_2	140	143	PF00928	0.393
TRG_ENDOCYTIC_2	302	305	PF00928	0.546
TRG_ENDOCYTIC_2	33	36	PF00928	0.420
TRG_ENDOCYTIC_2	361	364	PF00928	0.393
TRG_ENDOCYTIC_2	76	79	PF00928	0.368
TRG_ER_diArg_1	457	460	PF00400	0.654
TRG_ER_diArg_1	555	557	PF00400	0.556
TRG_ER_diArg_1	71	73	PF00400	0.514
TRG_NES_CRM1_1	386	399	PF08389	0.488
TRG_NES_CRM1_1	560	572	PF08389	0.454
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.505
TRG_Pf-PMV_PEXEL_1	223	227	PF00026	0.502
TRG_Pf-PMV_PEXEL_1	281	286	PF00026	0.448

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJU9	Leptomonas seymouri	47%	100%
A0A0S4JDR0	Bodo saltans	27%	86%
A0A1X0NY20	Trypanosomatidae	30%	100%
A0A3S7WVR9	Leishmania donovani	89%	100%
A0A422NEI4	Trypanosoma rangeli	30%	100%
A4HYM1	Leishmania infantum	89%	100%
C9ZHT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AI85	Leishmania braziliensis	73%	98%
Q4QD14	Leishmania major	89%	100%