Protein disulfide-isomerase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase 2

Species:

Leishmania mexicana

UniProt:

E9AUD1_LEIMU

TriTrypDb:

LmxM.36.6940

Length:

473

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 1
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 10, no: 1
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005788	endoplasmic reticulum lumen	5	10
GO:0031974	membrane-enclosed lumen	2	10
GO:0043233	organelle lumen	3	10
GO:0070013	intracellular organelle lumen	4	10
GO:0110165	cellular anatomical entity	1	11
GO:0005783	endoplasmic reticulum	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AUD1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUD1

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	1
GO:0006950	response to stress	2	1
GO:0009987	cellular process	1	1
GO:0033554	cellular response to stress	3	1
GO:0034976	response to endoplasmic reticulum stress	4	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1

Molecular functions
Term	Name	Level	Count
GO:0003756	protein disulfide isomerase activity	3	12
GO:0003824	catalytic activity	1	12
GO:0016853	isomerase activity	2	12
GO:0016860	intramolecular oxidoreductase activity	3	12
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	278	282	PF00656	0.505
CLV_NRD_NRD_1	309	311	PF00675	0.469
CLV_PCSK_KEX2_1	309	311	PF00082	0.479
CLV_PCSK_SKI1_1	141	145	PF00082	0.538
CLV_PCSK_SKI1_1	164	168	PF00082	0.347
CLV_PCSK_SKI1_1	3	7	PF00082	0.608
CLV_PCSK_SKI1_1	331	335	PF00082	0.522
CLV_PCSK_SKI1_1	394	398	PF00082	0.337
DEG_APCC_DBOX_1	2	10	PF00400	0.643
DEG_Nend_UBRbox_3	1	3	PF02207	0.655
DOC_MAPK_gen_1	140	148	PF00069	0.506
DOC_MAPK_gen_1	243	252	PF00069	0.505
DOC_MAPK_gen_1	270	277	PF00069	0.380
DOC_MAPK_MEF2A_6	89	96	PF00069	0.393
DOC_PP1_RVXF_1	243	250	PF00149	0.421
DOC_PP2B_LxvP_1	292	295	PF13499	0.443
DOC_PP4_FxxP_1	377	380	PF00568	0.358
DOC_PP4_FxxP_1	43	46	PF00568	0.393
DOC_USP7_MATH_1	319	323	PF00917	0.311
DOC_USP7_MATH_1	462	466	PF00917	0.748
DOC_USP7_UBL2_3	128	132	PF12436	0.527
DOC_USP7_UBL2_3	85	89	PF12436	0.402
DOC_WW_Pin1_4	181	186	PF00397	0.505
DOC_WW_Pin1_4	205	210	PF00397	0.389
DOC_WW_Pin1_4	315	320	PF00397	0.428
LIG_BIR_III_2	344	348	PF00653	0.577
LIG_eIF4E_1	271	277	PF01652	0.479
LIG_FHA_1	219	225	PF00498	0.478
LIG_FHA_1	354	360	PF00498	0.407
LIG_FHA_1	368	374	PF00498	0.358
LIG_FHA_1	91	97	PF00498	0.345
LIG_FHA_2	276	282	PF00498	0.481
LIG_FHA_2	410	416	PF00498	0.436
LIG_FHA_2	444	450	PF00498	0.426
LIG_FHA_2	68	74	PF00498	0.505
LIG_FHA_2	75	81	PF00498	0.390
LIG_GBD_Chelix_1	165	173	PF00786	0.430
LIG_GBD_Chelix_1	6	14	PF00786	0.646
LIG_LIR_Apic_2	105	109	PF02991	0.443
LIG_LIR_Apic_2	428	433	PF02991	0.358
LIG_LIR_Gen_1	28	38	PF02991	0.418
LIG_LIR_Gen_1	4	13	PF02991	0.638
LIG_LIR_Nem_3	225	230	PF02991	0.407
LIG_LIR_Nem_3	234	238	PF02991	0.405
LIG_LIR_Nem_3	28	33	PF02991	0.418
LIG_LIR_Nem_3	283	288	PF02991	0.437
LIG_LIR_Nem_3	297	303	PF02991	0.285
LIG_LIR_Nem_3	362	366	PF02991	0.469
LIG_LIR_Nem_3	4	8	PF02991	0.640
LIG_LIR_Nem_3	424	429	PF02991	0.374
LIG_NRBOX	9	15	PF00104	0.598
LIG_PCNA_PIPBox_1	212	221	PF02747	0.214
LIG_PDZ_Class_3	468	473	PF00595	0.729
LIG_Pex14_2	377	381	PF04695	0.355
LIG_Pex14_2	43	47	PF04695	0.378
LIG_SH2_CRK	106	110	PF00017	0.479
LIG_SH2_CRK	285	289	PF00017	0.505
LIG_SH2_NCK_1	392	396	PF00017	0.426
LIG_SH2_SRC	279	282	PF00017	0.389
LIG_SH2_STAP1	392	396	PF00017	0.415
LIG_SH2_STAT3	271	274	PF00017	0.479
LIG_SH2_STAT5	204	207	PF00017	0.467
LIG_SH2_STAT5	279	282	PF00017	0.389
LIG_SH2_STAT5	332	335	PF00017	0.558
LIG_SH2_STAT5	366	369	PF00017	0.415
LIG_SH2_STAT5	430	433	PF00017	0.370
LIG_SH2_STAT5	95	98	PF00017	0.505
LIG_SUMO_SIM_anti_2	37	42	PF11976	0.417
LIG_SUMO_SIM_par_1	263	269	PF11976	0.428
LIG_TRAF2_1	468	471	PF00917	0.729
LIG_TRAF2_1	77	80	PF00917	0.428
LIG_WRC_WIRS_1	215	220	PF05994	0.377
MOD_CK1_1	126	132	PF00069	0.320
MOD_CK1_1	153	159	PF00069	0.558
MOD_CK1_1	168	174	PF00069	0.388
MOD_CK1_1	214	220	PF00069	0.365
MOD_CK1_1	364	370	PF00069	0.292
MOD_CK1_1	398	404	PF00069	0.428
MOD_CK1_1	456	462	PF00069	0.594
MOD_CK1_1	465	471	PF00069	0.696
MOD_CK2_1	465	471	PF00069	0.735
MOD_CK2_1	67	73	PF00069	0.496
MOD_CK2_1	74	80	PF00069	0.461
MOD_GlcNHglycan	152	155	PF01048	0.468
MOD_GlcNHglycan	18	21	PF01048	0.613
MOD_GlcNHglycan	34	37	PF01048	0.479
MOD_GlcNHglycan	422	426	PF01048	0.384
MOD_GlcNHglycan	460	463	PF01048	0.660
MOD_GSK3_1	160	167	PF00069	0.274
MOD_GSK3_1	214	221	PF00069	0.449
MOD_GSK3_1	315	322	PF00069	0.377
MOD_GSK3_1	32	39	PF00069	0.465
MOD_GSK3_1	421	428	PF00069	0.372
MOD_GSK3_1	458	465	PF00069	0.679
MOD_NEK2_1	1	6	PF00069	0.671
MOD_NEK2_1	160	165	PF00069	0.369
MOD_NEK2_1	218	223	PF00069	0.380
MOD_NEK2_1	260	265	PF00069	0.426
MOD_NEK2_1	409	414	PF00069	0.409
MOD_NEK2_1	421	426	PF00069	0.333
MOD_NEK2_1	453	458	PF00069	0.594
MOD_PIKK_1	168	174	PF00454	0.439
MOD_PIKK_1	270	276	PF00454	0.462
MOD_PIKK_1	334	340	PF00454	0.345
MOD_PIKK_1	367	373	PF00454	0.428
MOD_Plk_1	179	185	PF00069	0.285
MOD_Plk_1	36	42	PF00069	0.455
MOD_Plk_4	114	120	PF00069	0.457
MOD_Plk_4	190	196	PF00069	0.373
MOD_Plk_4	214	220	PF00069	0.377
MOD_Plk_4	260	266	PF00069	0.490
MOD_Plk_4	275	281	PF00069	0.384
MOD_Plk_4	337	343	PF00069	0.565
MOD_Plk_4	354	360	PF00069	0.417
MOD_Plk_4	36	42	PF00069	0.411
MOD_Plk_4	425	431	PF00069	0.372
MOD_Plk_4	90	96	PF00069	0.359
MOD_ProDKin_1	181	187	PF00069	0.505
MOD_ProDKin_1	205	211	PF00069	0.389
MOD_ProDKin_1	315	321	PF00069	0.428
MOD_SUMO_for_1	139	142	PF00179	0.493
MOD_SUMO_for_1	333	336	PF00179	0.586
MOD_SUMO_rev_2	296	301	PF00179	0.381
MOD_SUMO_rev_2	84	91	PF00179	0.489
TRG_ENDOCYTIC_2	285	288	PF00928	0.443
TRG_ENDOCYTIC_2	392	395	PF00928	0.415

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P805	Leptomonas seymouri	65%	99%
A0A0S4KL52	Bodo saltans	39%	100%
A0A1X0NMX9	Trypanosomatidae	46%	98%
A0A3Q8IS34	Leishmania donovani	88%	99%
A0A3R7L858	Trypanosoma rangeli	45%	98%
A3KPF5	Arabidopsis thaliana	25%	88%
A4HQL6	Leishmania braziliensis	77%	100%
A4ICD5	Leishmania infantum	87%	99%
D0A3S0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	95%
D3Z6P0	Mus musculus	30%	90%
D4B2L8	Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371)	34%	90%
P04785	Rattus norvegicus	30%	93%
P05307	Bos taurus	29%	93%
P07237	Homo sapiens	29%	93%
P08003	Mus musculus	31%	74%
P09102	Gallus gallus	29%	92%
P09103	Mus musculus	30%	93%
P11598	Rattus norvegicus	32%	94%
P12865	Trypanosoma brucei brucei	36%	95%
P13667	Homo sapiens	33%	73%
P17967	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	91%
P21195	Oryctolagus cuniculus	29%	93%
P27773	Mus musculus	32%	94%
P29828	Medicago sativa	32%	92%
P30101	Homo sapiens	31%	94%
P32474	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	24%	91%
P34329	Caenorhabditis elegans	31%	77%
P38657	Bos taurus	32%	94%
P38658	Schistosoma mansoni	30%	98%
P38659	Rattus norvegicus	31%	74%
P52588	Zea mays	32%	92%
P52589	Triticum aestivum	33%	92%
P54399	Drosophila melanogaster	31%	95%
P55059	Humicola insolens	35%	94%
P80284	Hordeum vulgare	33%	92%
Q00002	Alternaria alternata	26%	100%
Q00248	Aspergillus oryzae (strain ATCC 42149 / RIB 40)	35%	92%
Q10057	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	96%
Q12730	Aspergillus niger	32%	92%
Q13087	Homo sapiens	32%	90%
Q17770	Caenorhabditis elegans	30%	96%
Q17967	Caenorhabditis elegans	30%	98%
Q29RV1	Bos taurus	32%	74%
Q2HWU2	Macaca fuscata fuscata	29%	93%
Q43116	Ricinus communis	32%	95%
Q4Q059	Leishmania major	87%	100%
Q4VIT4	Chlorocebus aethiops	31%	94%
Q53LQ0	Oryza sativa subsp. japonica	32%	92%
Q54EN4	Dictyostelium discoideum	30%	92%
Q5R5B6	Pongo abelii	29%	93%
Q5RCH2	Pongo abelii	33%	90%
Q5RDG4	Pongo abelii	31%	94%
Q5WA72	Oryza sativa subsp. japonica	26%	89%
Q5XI02	Rattus norvegicus	20%	80%
Q66GQ3	Arabidopsis thaliana	27%	89%
Q67IX6	Oryza sativa subsp. japonica	29%	84%
Q69ST6	Oryza sativa subsp. japonica	29%	87%
Q7XRB5	Oryza sativa subsp. japonica	29%	91%
Q8JG64	Gallus gallus	32%	94%
Q8N807	Homo sapiens	20%	81%
Q8R4U2	Cricetulus griseus	29%	93%
Q8VX13	Arabidopsis thaliana	30%	82%
Q9DAN1	Mus musculus	21%	80%
Q9FF55	Arabidopsis thaliana	28%	79%
Q9SRG3	Arabidopsis thaliana	33%	93%
Q9XF61	Datisca glomerata	30%	93%
Q9XI01	Arabidopsis thaliana	33%	94%
V5BHP7	Trypanosoma cruzi	43%	100%