Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9AUA2_LEIMU

TriTrypDb:

LmxM.36.6660

Length:

400

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

E9AUA2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AUA2

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006370	7-methylguanosine mRNA capping	8	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009452	7-methylguanosine RNA capping	8	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0016556	mRNA modification	6	12
GO:0032259	methylation	2	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0036260	RNA capping	7	12
GO:0036451	cap mRNA methylation	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0080009	mRNA methylation	5	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0097309	cap1 mRNA methylation	7	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003824	catalytic activity	1	12
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	12
GO:0005488	binding	1	10
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008174	mRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	10
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	144	146	PF00675	0.272
CLV_NRD_NRD_1	15	17	PF00675	0.478
CLV_NRD_NRD_1	244	246	PF00675	0.313
CLV_NRD_NRD_1	274	276	PF00675	0.246
CLV_PCSK_KEX2_1	118	120	PF00082	0.310
CLV_PCSK_KEX2_1	144	146	PF00082	0.266
CLV_PCSK_KEX2_1	15	17	PF00082	0.479
CLV_PCSK_KEX2_1	274	276	PF00082	0.322
CLV_PCSK_KEX2_1	379	381	PF00082	0.497
CLV_PCSK_PC1ET2_1	118	120	PF00082	0.310
CLV_PCSK_PC1ET2_1	379	381	PF00082	0.497
CLV_PCSK_SKI1_1	115	119	PF00082	0.161
CLV_PCSK_SKI1_1	263	267	PF00082	0.217
CLV_PCSK_SKI1_1	31	35	PF00082	0.407
DEG_APCC_DBOX_1	49	57	PF00400	0.475
DEG_APCC_DBOX_1	71	79	PF00400	0.395
DEG_Nend_UBRbox_1	1	4	PF02207	0.506
DOC_MAPK_DCC_7	72	80	PF00069	0.400
DOC_MAPK_gen_1	72	80	PF00069	0.493
DOC_MAPK_MEF2A_6	202	210	PF00069	0.458
DOC_MAPK_MEF2A_6	291	298	PF00069	0.442
DOC_PP1_RVXF_1	6	13	PF00149	0.380
DOC_USP7_MATH_1	159	163	PF00917	0.573
DOC_USP7_MATH_1	24	28	PF00917	0.460
DOC_USP7_MATH_1	281	285	PF00917	0.431
DOC_USP7_MATH_2	302	308	PF00917	0.448
DOC_USP7_UBL2_3	242	246	PF12436	0.513
DOC_USP7_UBL2_3	375	379	PF12436	0.627
DOC_WW_Pin1_4	170	175	PF00397	0.535
DOC_WW_Pin1_4	256	261	PF00397	0.431
DOC_WW_Pin1_4	387	392	PF00397	0.762
LIG_14-3-3_CanoR_1	113	118	PF00244	0.445
LIG_14-3-3_CanoR_1	121	126	PF00244	0.464
LIG_14-3-3_CanoR_1	285	294	PF00244	0.457
LIG_BIR_III_2	363	367	PF00653	0.475
LIG_BRCT_BRCA1_1	172	176	PF00533	0.458
LIG_BRCT_BRCA1_1	8	12	PF00533	0.505
LIG_Clathr_ClatBox_1	293	297	PF01394	0.535
LIG_deltaCOP1_diTrp_1	304	313	PF00928	0.401
LIG_deltaCOP1_diTrp_1	331	339	PF00928	0.463
LIG_FHA_1	177	183	PF00498	0.438
LIG_FHA_1	350	356	PF00498	0.426
LIG_FHA_1	367	373	PF00498	0.376
LIG_FHA_1	388	394	PF00498	0.488
LIG_FHA_2	2	8	PF00498	0.515
LIG_FHA_2	56	62	PF00498	0.407
LIG_LIR_Gen_1	102	112	PF02991	0.456
LIG_LIR_Gen_1	124	132	PF02991	0.474
LIG_LIR_Gen_1	165	174	PF02991	0.442
LIG_LIR_Nem_3	102	108	PF02991	0.472
LIG_LIR_Nem_3	124	128	PF02991	0.474
LIG_LIR_Nem_3	165	170	PF02991	0.442
LIG_LIR_Nem_3	256	261	PF02991	0.449
LIG_LIR_Nem_3	297	301	PF02991	0.548
LIG_LIR_Nem_3	303	309	PF02991	0.456
LIG_LIR_Nem_3	311	316	PF02991	0.426
LIG_MYND_1	325	329	PF01753	0.364
LIG_NRBOX	107	113	PF00104	0.579
LIG_Pex14_1	38	42	PF04695	0.332
LIG_Pex14_2	215	219	PF04695	0.442
LIG_Rb_pABgroove_1	249	257	PF01858	0.431
LIG_SH2_CRK	273	277	PF00017	0.442
LIG_SH2_STAP1	167	171	PF00017	0.482
LIG_SH2_STAT5	267	270	PF00017	0.445
LIG_SH2_STAT5	292	295	PF00017	0.466
LIG_SH2_STAT5	309	312	PF00017	0.376
LIG_SH2_STAT5	68	71	PF00017	0.395
LIG_SH3_3	275	281	PF00018	0.460
LIG_SUMO_SIM_anti_2	58	64	PF11976	0.387
LIG_SUMO_SIM_par_1	206	212	PF11976	0.421
LIG_SUMO_SIM_par_1	76	81	PF11976	0.422
LIG_WRC_WIRS_1	255	260	PF05994	0.442
MOD_CDK_SPxxK_3	256	263	PF00069	0.431
MOD_CK1_1	259	265	PF00069	0.474
MOD_CK1_1	98	104	PF00069	0.535
MOD_Cter_Amidation	142	145	PF01082	0.278
MOD_GlcNHglycan	161	164	PF01048	0.340
MOD_GlcNHglycan	80	83	PF01048	0.469
MOD_GSK3_1	170	177	PF00069	0.466
MOD_GSK3_1	300	307	PF00069	0.333
MOD_GSK3_1	345	352	PF00069	0.435
MOD_GSK3_1	64	71	PF00069	0.479
MOD_GSK3_1	95	102	PF00069	0.494
MOD_N-GLC_1	121	126	PF02516	0.160
MOD_N-GLC_1	85	90	PF02516	0.438
MOD_NEK2_1	106	111	PF00069	0.463
MOD_NEK2_1	176	181	PF00069	0.498
MOD_NEK2_1	254	259	PF00069	0.438
MOD_NEK2_1	266	271	PF00069	0.419
MOD_NEK2_1	99	104	PF00069	0.464
MOD_NEK2_2	26	31	PF00069	0.529
MOD_PIKK_1	133	139	PF00454	0.431
MOD_PIKK_1	55	61	PF00454	0.412
MOD_PKA_2	281	287	PF00069	0.442
MOD_PKB_1	119	127	PF00069	0.360
MOD_Plk_1	121	127	PF00069	0.360
MOD_Plk_1	217	223	PF00069	0.478
MOD_Plk_1	6	12	PF00069	0.365
MOD_Plk_2-3	6	12	PF00069	0.504
MOD_ProDKin_1	170	176	PF00069	0.535
MOD_ProDKin_1	256	262	PF00069	0.431
MOD_ProDKin_1	387	393	PF00069	0.766
MOD_SUMO_rev_2	370	374	PF00179	0.539
TRG_DiLeu_BaEn_1	107	112	PF01217	0.478
TRG_ENDOCYTIC_2	167	170	PF00928	0.431
TRG_ENDOCYTIC_2	273	276	PF00928	0.451
TRG_ER_diArg_1	14	16	PF00400	0.474
TRG_ER_diArg_1	273	275	PF00400	0.538
TRG_NES_CRM1_1	51	66	PF08389	0.378
TRG_NLS_MonoCore_2	117	122	PF00514	0.360
TRG_NLS_MonoExtN_4	115	122	PF00514	0.518

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P800	Leptomonas seymouri	77%	99%
A0A0S4IIT9	Bodo saltans	49%	98%
A0A1X0NL91	Trypanosomatidae	55%	100%
A0A3S5IS12	Trypanosoma rangeli	54%	100%
A0A3S7XCC2	Leishmania donovani	94%	100%
A4HQI9	Leishmania braziliensis	83%	100%
D0A3N7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
E9AHZ8	Leishmania infantum	94%	100%
Q38AH0	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	50%	100%
Q4E123	Trypanosoma cruzi (strain CL Brener)	55%	100%
Q4Q089	Leishmania major	94%	100%
V5BD10	Trypanosoma cruzi	54%	100%