Methylenetetrahydrofolate reductase (NAD(P)H)

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methylenetetrahydrofolate reductase, putative

Species:

Leishmania mexicana

UniProt:

E9AU75_LEIMU

TriTrypDb:

LmxM.36.6390

Length:

309

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AU75

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AU75

Function

Biological processes
Term	Name	Level	Count
GO:0000096	sulfur amino acid metabolic process	4	9
GO:0006082	organic acid metabolic process	3	9
GO:0006520	amino acid metabolic process	3	9
GO:0006555	methionine metabolic process	5	9
GO:0006575	cellular modified amino acid metabolic process	3	9
GO:0006725	cellular aromatic compound metabolic process	3	9
GO:0006730	one-carbon metabolic process	3	9
GO:0006760	folic acid-containing compound metabolic process	4	9
GO:0006790	sulfur compound metabolic process	3	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009066	aspartate family amino acid metabolic process	5	9
GO:0009987	cellular process	1	9
GO:0019752	carboxylic acid metabolic process	5	9
GO:0035999	tetrahydrofolate interconversion	4	9
GO:0042558	pteridine-containing compound metabolic process	4	9
GO:0043436	oxoacid metabolic process	4	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0044281	small molecule metabolic process	2	9
GO:0046483	heterocycle metabolic process	3	9
GO:0046653	tetrahydrofolate metabolic process	5	9
GO:0071704	organic substance metabolic process	2	9
GO:1901360	organic cyclic compound metabolic process	3	9
GO:1901564	organonitrogen compound metabolic process	3	9
GO:1901605	alpha-amino acid metabolic process	4	9
GO:0000097	sulfur amino acid biosynthetic process	5	1
GO:0008652	amino acid biosynthetic process	4	1
GO:0009058	biosynthetic process	2	1
GO:0009067	aspartate family amino acid biosynthetic process	6	1
GO:0009086	methionine biosynthetic process	6	1
GO:0016053	organic acid biosynthetic process	4	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044272	sulfur compound biosynthetic process	4	1
GO:0044283	small molecule biosynthetic process	3	1
GO:0046394	carboxylic acid biosynthetic process	5	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1
GO:1901607	alpha-amino acid biosynthetic process	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004489	methylenetetrahydrofolate reductase (NAD(P)H) activity	5	9
GO:0016491	oxidoreductase activity	2	9
GO:0016645	oxidoreductase activity, acting on the CH-NH group of donors	3	9
GO:0016646	oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor	4	9
GO:0000166	nucleotide binding	3	1
GO:0005488	binding	1	1
GO:0036094	small molecule binding	2	1
GO:0043167	ion binding	2	1
GO:0043168	anion binding	3	1
GO:0050660	flavin adenine dinucleotide binding	4	1
GO:0071949	FAD binding	5	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901265	nucleoside phosphate binding	3	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	183	187	PF00656	0.318
CLV_C14_Caspase3-7	256	260	PF00656	0.274
CLV_PCSK_KEX2_1	208	210	PF00082	0.274
CLV_PCSK_PC1ET2_1	208	210	PF00082	0.274
CLV_PCSK_SKI1_1	108	112	PF00082	0.315
CLV_PCSK_SKI1_1	205	209	PF00082	0.310
CLV_PCSK_SKI1_1	299	303	PF00082	0.329
CLV_PCSK_SKI1_1	96	100	PF00082	0.290
DEG_APCC_KENBOX_2	254	258	PF00400	0.318
DOC_CKS1_1	77	82	PF01111	0.318
DOC_MAPK_gen_1	205	215	PF00069	0.264
DOC_MAPK_MEF2A_6	208	217	PF00069	0.298
DOC_MAPK_MEF2A_6	45	53	PF00069	0.227
DOC_PP4_FxxP_1	23	26	PF00568	0.187
DOC_USP7_MATH_1	187	191	PF00917	0.318
DOC_USP7_UBL2_3	92	96	PF12436	0.209
DOC_WW_Pin1_4	44	49	PF00397	0.337
DOC_WW_Pin1_4	76	81	PF00397	0.318
DOC_WW_Pin1_4	87	92	PF00397	0.216
LIG_14-3-3_CanoR_1	234	239	PF00244	0.347
LIG_14-3-3_CanoR_1	60	68	PF00244	0.258
LIG_BIR_II_1	1	5	PF00653	0.398
LIG_BRCT_BRCA1_1	19	23	PF00533	0.187
LIG_BRCT_BRCA1_1	46	50	PF00533	0.287
LIG_EH1_1	104	112	PF00400	0.318
LIG_FHA_1	102	108	PF00498	0.404
LIG_FHA_1	214	220	PF00498	0.347
LIG_FHA_1	295	301	PF00498	0.224
LIG_FHA_1	63	69	PF00498	0.292
LIG_FHA_2	141	147	PF00498	0.347
LIG_FHA_2	166	172	PF00498	0.212
LIG_FHA_2	17	23	PF00498	0.347
LIG_Integrin_RGD_1	114	116	PF01839	0.253
LIG_LIR_Apic_2	20	26	PF02991	0.187
LIG_LIR_Gen_1	201	207	PF02991	0.287
LIG_LIR_Gen_1	282	291	PF02991	0.318
LIG_LIR_Gen_1	47	57	PF02991	0.271
LIG_LIR_Nem_3	142	147	PF02991	0.298
LIG_LIR_Nem_3	19	24	PF02991	0.310
LIG_LIR_Nem_3	201	206	PF02991	0.291
LIG_LIR_Nem_3	227	232	PF02991	0.331
LIG_LIR_Nem_3	282	286	PF02991	0.318
LIG_LIR_Nem_3	297	301	PF02991	0.178
LIG_LIR_Nem_3	47	53	PF02991	0.314
LIG_Pex14_1	17	21	PF04695	0.347
LIG_SH2_STAP1	198	202	PF00017	0.268
LIG_SH2_STAT5	139	142	PF00017	0.265
LIG_SH2_STAT5	147	150	PF00017	0.238
LIG_SH2_STAT5	18	21	PF00017	0.258
LIG_SH2_STAT5	180	183	PF00017	0.253
LIG_SH2_STAT5	203	206	PF00017	0.359
LIG_SH2_STAT5	289	292	PF00017	0.290
LIG_SH3_3	35	41	PF00018	0.347
LIG_SH3_3	74	80	PF00018	0.371
LIG_TYR_ITIM	281	286	PF00017	0.318
LIG_WRC_WIRS_1	40	45	PF05994	0.161
LIG_WW_3	24	28	PF00397	0.253
MOD_CDK_SPK_2	87	92	PF00069	0.274
MOD_CK1_1	242	248	PF00069	0.413
MOD_CK1_1	294	300	PF00069	0.264
MOD_CK1_1	62	68	PF00069	0.301
MOD_CK2_1	16	22	PF00069	0.287
MOD_GlcNHglycan	278	281	PF01048	0.318
MOD_GlcNHglycan	293	296	PF01048	0.178
MOD_GSK3_1	187	194	PF00069	0.375
MOD_GSK3_1	234	241	PF00069	0.283
MOD_GSK3_1	287	294	PF00069	0.347
MOD_GSK3_1	58	65	PF00069	0.285
MOD_GSK3_1	83	90	PF00069	0.334
MOD_N-GLC_1	291	296	PF02516	0.347
MOD_NEK2_1	148	153	PF00069	0.327
MOD_NEK2_1	191	196	PF00069	0.274
MOD_NEK2_1	213	218	PF00069	0.198
MOD_NEK2_1	238	243	PF00069	0.253
MOD_NEK2_1	291	296	PF00069	0.375
MOD_NEK2_1	51	56	PF00069	0.309
MOD_NEK2_1	83	88	PF00069	0.253
MOD_NEK2_1	99	104	PF00069	0.253
MOD_NEK2_2	198	203	PF00069	0.347
MOD_PIKK_1	191	197	PF00454	0.253
MOD_PKA_2	276	282	PF00069	0.321
MOD_PKA_2	59	65	PF00069	0.304
MOD_Plk_1	198	204	PF00069	0.347
MOD_Plk_1	51	57	PF00069	0.287
MOD_Plk_1	62	68	PF00069	0.253
MOD_Plk_4	187	193	PF00069	0.420
MOD_Plk_4	198	204	PF00069	0.360
MOD_Plk_4	39	45	PF00069	0.216
MOD_ProDKin_1	44	50	PF00069	0.337
MOD_ProDKin_1	76	82	PF00069	0.318
MOD_ProDKin_1	87	93	PF00069	0.216
TRG_ENDOCYTIC_2	147	150	PF00928	0.327
TRG_ENDOCYTIC_2	203	206	PF00928	0.373
TRG_ENDOCYTIC_2	229	232	PF00928	0.287
TRG_ENDOCYTIC_2	283	286	PF00928	0.318
TRG_Pf-PMV_PEXEL_1	138	142	PF00026	0.355

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IKP8	Leptomonas seymouri	67%	100%
A0A0S4IQL6	Bodo saltans	53%	99%
A0A1X0NM13	Trypanosomatidae	55%	100%
A0A3Q8IJZ9	Leishmania donovani	91%	100%
A4HQF9	Leishmania braziliensis	86%	100%
A4IC95	Leishmania infantum	91%	100%
O54235	Streptomyces lividans	33%	100%
O67422	Aquifex aeolicus (strain VF5)	36%	100%
P0AEZ1	Escherichia coli (strain K12)	33%	100%
P0AEZ2	Shigella flexneri	33%	100%
P11003	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	31%	100%
P45208	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	33%	100%
P57154	Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS)	31%	100%
P71319	Pectobacterium carotovorum subsp. carotovorum	32%	100%
Q4Q0B9	Leishmania major	90%	100%
Q89B13	Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp)	30%	100%
Q8KA62	Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)	30%	100%
Q9JZQ3	Neisseria meningitidis serogroup B (strain MC58)	33%	100%