Ubiquitin thioesterase OTU1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

OTU-like cysteine protease, putative

Species:

Leishmania mexicana

UniProt:

E9AU37_LEIMU

TriTrypDb:

LmxM.36.6020

Length:

268

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9AU37

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AU37

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	12
GO:0006511	ubiquitin-dependent protein catabolic process	7	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0008152	metabolic process	1	12
GO:0009056	catabolic process	2	12
GO:0009057	macromolecule catabolic process	4	12
GO:0009987	cellular process	1	12
GO:0010033	response to organic substance	3	12
GO:0010243	response to organonitrogen compound	4	12
GO:0010498	proteasomal protein catabolic process	5	12
GO:0016579	protein deubiquitination	6	12
GO:0019538	protein metabolic process	3	12
GO:0019941	modification-dependent protein catabolic process	6	12
GO:0030163	protein catabolic process	4	12
GO:0030433	ubiquitin-dependent ERAD pathway	6	12
GO:0033554	cellular response to stress	3	12
GO:0034976	response to endoplasmic reticulum stress	4	12
GO:0036211	protein modification process	4	12
GO:0036503	ERAD pathway	5	12
GO:0042221	response to chemical	2	12
GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process	6	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043632	modification-dependent macromolecule catabolic process	5	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044248	cellular catabolic process	3	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0044265	obsolete cellular macromolecule catabolic process	4	12
GO:0050896	response to stimulus	1	12
GO:0051603	proteolysis involved in protein catabolic process	5	12
GO:0051716	cellular response to stimulus	2	12
GO:0070646	protein modification by small protein removal	5	12
GO:0070647	protein modification by small protein conjugation or removal	5	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901565	organonitrogen compound catabolic process	4	12
GO:1901575	organic substance catabolic process	3	12
GO:1901698	response to nitrogen compound	3	12
GO:0007165	signal transduction	2	1
GO:0030968	endoplasmic reticulum unfolded protein response	3	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0065007	biological regulation	1	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004843	cysteine-type deubiquitinase activity	5	12
GO:0008233	peptidase activity	3	12
GO:0008234	cysteine-type peptidase activity	4	12
GO:0016787	hydrolase activity	2	12
GO:0019783	ubiquitin-like protein peptidase activity	4	12
GO:0101005	deubiquitinase activity	5	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	105	107	PF00675	0.323
CLV_NRD_NRD_1	34	36	PF00675	0.383
CLV_PCSK_FUR_1	250	254	PF00082	0.414
CLV_PCSK_KEX2_1	104	106	PF00082	0.332
CLV_PCSK_KEX2_1	252	254	PF00082	0.449
CLV_PCSK_PC1ET2_1	252	254	PF00082	0.480
CLV_PCSK_SKI1_1	139	143	PF00082	0.421
CLV_PCSK_SKI1_1	209	213	PF00082	0.397
CLV_PCSK_SKI1_1	64	68	PF00082	0.311
DEG_SCF_FBW7_1	7	13	PF00400	0.457
DEG_SPOP_SBC_1	10	14	PF00917	0.488
DOC_CKS1_1	7	12	PF01111	0.595
DOC_CYCLIN_RxL_1	61	72	PF00134	0.262
DOC_MAPK_gen_1	35	41	PF00069	0.371
DOC_MAPK_gen_1	67	76	PF00069	0.454
DOC_USP7_MATH_1	10	14	PF00917	0.485
DOC_USP7_MATH_1	109	113	PF00917	0.453
DOC_USP7_MATH_1	159	163	PF00917	0.412
DOC_USP7_MATH_1	227	231	PF00917	0.321
DOC_WW_Pin1_4	58	63	PF00397	0.519
DOC_WW_Pin1_4	6	11	PF00397	0.541
LIG_14-3-3_CanoR_1	35	41	PF00244	0.444
LIG_14-3-3_CanoR_1	8	18	PF00244	0.433
LIG_BIR_II_1	1	5	PF00653	0.401
LIG_BRCT_BRCA1_1	111	115	PF00533	0.270
LIG_FHA_2	23	29	PF00498	0.488
LIG_LIR_Apic_2	90	95	PF02991	0.447
LIG_PCNA_yPIPBox_3	134	142	PF02747	0.379
LIG_PTB_Apo_2	222	229	PF02174	0.362
LIG_PTB_Phospho_1	222	228	PF10480	0.378
LIG_SH2_CRK	219	223	PF00017	0.265
LIG_SH2_NCK_1	219	223	PF00017	0.265
LIG_SH2_PTP2	92	95	PF00017	0.395
LIG_SH2_SRC	92	95	PF00017	0.422
LIG_SH2_STAP1	160	164	PF00017	0.391
LIG_SH2_STAP1	206	210	PF00017	0.288
LIG_SH2_STAP1	219	223	PF00017	0.280
LIG_SH2_STAT5	120	123	PF00017	0.274
LIG_SH2_STAT5	206	209	PF00017	0.342
LIG_SH2_STAT5	214	217	PF00017	0.330
LIG_SH2_STAT5	92	95	PF00017	0.422
LIG_SH3_1	91	97	PF00018	0.528
LIG_SH3_2	94	99	PF14604	0.476
LIG_SH3_3	91	97	PF00018	0.528
LIG_SUMO_SIM_anti_2	175	180	PF11976	0.275
LIG_SUMO_SIM_par_1	72	78	PF11976	0.373
LIG_UBA3_1	178	184	PF00899	0.405
LIG_WRC_WIRS_1	178	183	PF05994	0.284
MOD_CDC14_SPxK_1	61	64	PF00782	0.257
MOD_CDK_SPK_2	6	11	PF00069	0.444
MOD_CDK_SPxK_1	58	64	PF00069	0.280
MOD_CK1_1	162	168	PF00069	0.373
MOD_CK1_1	22	28	PF00069	0.534
MOD_CK1_1	34	40	PF00069	0.275
MOD_CK2_1	10	16	PF00069	0.613
MOD_CK2_1	123	129	PF00069	0.391
MOD_CK2_1	22	28	PF00069	0.501
MOD_CK2_1	227	233	PF00069	0.394
MOD_CK2_1	239	245	PF00069	0.406
MOD_GlcNHglycan	22	25	PF01048	0.568
MOD_GSK3_1	162	169	PF00069	0.482
MOD_GSK3_1	235	242	PF00069	0.454
MOD_GSK3_1	6	13	PF00069	0.475
MOD_N-GLC_1	20	25	PF02516	0.467
MOD_N-GLC_1	228	233	PF02516	0.346
MOD_N-GLC_2	111	113	PF02516	0.282
MOD_NEK2_1	1	6	PF00069	0.465
MOD_NEK2_1	20	25	PF00069	0.444
MOD_NEK2_1	204	209	PF00069	0.361
MOD_NEK2_1	212	217	PF00069	0.340
MOD_OFUCOSY	107	113	PF10250	0.325
MOD_PKA_2	34	40	PF00069	0.434
MOD_Plk_1	109	115	PF00069	0.292
MOD_Plk_1	149	155	PF00069	0.364
MOD_Plk_1	235	241	PF00069	0.451
MOD_Plk_4	159	165	PF00069	0.502
MOD_Plk_4	177	183	PF00069	0.290
MOD_Plk_4	235	241	PF00069	0.445
MOD_ProDKin_1	58	64	PF00069	0.522
MOD_ProDKin_1	6	12	PF00069	0.544
MOD_SUMO_rev_2	258	268	PF00179	0.426
MOD_SUMO_rev_2	78	83	PF00179	0.588
TRG_DiLeu_BaEn_1	28	33	PF01217	0.401
TRG_DiLeu_BaEn_1	70	75	PF01217	0.257
TRG_DiLeu_BaEn_2	176	182	PF01217	0.416
TRG_ENDOCYTIC_2	219	222	PF00928	0.268
TRG_ER_diArg_1	104	106	PF00400	0.363
TRG_ER_diArg_1	43	46	PF00400	0.375
TRG_ER_diArg_1	5	8	PF00400	0.407

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P901	Leptomonas seymouri	78%	100%
A0A0S4IPY5	Bodo saltans	47%	100%
A0A1X0NNB5	Trypanosomatidae	51%	100%
A0A3R7LXZ2	Trypanosoma rangeli	56%	86%
A0A3S7XC16	Leishmania donovani	96%	70%
A4HQC1	Leishmania braziliensis	87%	100%
A4IE15	Leishmania infantum	96%	70%
D0A3Y1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	100%
O13974	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	81%
P43558	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	89%
Q05B57	Bos taurus	35%	77%
Q0IH43	Xenopus laevis	32%	88%
Q32Q05	Rattus norvegicus	35%	78%
Q4Q0F8	Leishmania major	95%	100%
Q55BI3	Dictyostelium discoideum	33%	82%
Q567B1	Danio rerio	33%	89%
Q5F3A6	Gallus gallus	37%	89%
Q5VVQ6	Homo sapiens	35%	77%
Q8CB27	Mus musculus	35%	78%
Q9VRJ9	Drosophila melanogaster	28%	77%
V5B3J2	Trypanosoma cruzi	54%	100%