EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AT90_LEIMU

TriTrypDb:

LmxM.36.3120

Length:

563

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	1
GO:0031514	motile cilium	5	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0120025	plasma membrane bounded cell projection	3	1
GO:0016020	membrane	2	1

Expansion

Sequence features

E9AT90

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AT90

Function

Biological processes
Term	Name	Level	Count
GO:0006996	organelle organization	4	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044782	cilium organization	5	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	5
GO:0005509	calcium ion binding	5	5
GO:0043167	ion binding	2	5
GO:0043169	cation binding	3	5
GO:0046872	metal ion binding	4	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	273	277	PF00656	0.421
CLV_C14_Caspase3-7	437	441	PF00656	0.454
CLV_NRD_NRD_1	344	346	PF00675	0.373
CLV_NRD_NRD_1	352	354	PF00675	0.353
CLV_NRD_NRD_1	538	540	PF00675	0.576
CLV_PCSK_FUR_1	342	346	PF00082	0.460
CLV_PCSK_KEX2_1	344	346	PF00082	0.339
CLV_PCSK_KEX2_1	373	375	PF00082	0.184
CLV_PCSK_KEX2_1	543	545	PF00082	0.494
CLV_PCSK_PC1ET2_1	373	375	PF00082	0.240
CLV_PCSK_PC1ET2_1	543	545	PF00082	0.578
CLV_PCSK_PC7_1	539	545	PF00082	0.462
CLV_PCSK_SKI1_1	161	165	PF00082	0.227
CLV_PCSK_SKI1_1	235	239	PF00082	0.395
CLV_PCSK_SKI1_1	344	348	PF00082	0.369
CLV_PCSK_SKI1_1	45	49	PF00082	0.570
CLV_PCSK_SKI1_1	512	516	PF00082	0.412
CLV_PCSK_SKI1_1	53	57	PF00082	0.400
CLV_PCSK_SKI1_1	540	544	PF00082	0.456
CLV_PCSK_SKI1_1	552	556	PF00082	0.424
DEG_SPOP_SBC_1	285	289	PF00917	0.727
DOC_MAPK_FxFP_2	208	211	PF00069	0.419
DOC_MAPK_gen_1	257	264	PF00069	0.598
DOC_MAPK_gen_1	313	321	PF00069	0.363
DOC_MAPK_gen_1	353	361	PF00069	0.425
DOC_MAPK_gen_1	539	547	PF00069	0.412
DOC_MAPK_HePTP_8	83	95	PF00069	0.398
DOC_MAPK_MEF2A_6	294	301	PF00069	0.478
DOC_MAPK_MEF2A_6	328	336	PF00069	0.467
DOC_MAPK_MEF2A_6	354	363	PF00069	0.411
DOC_MAPK_MEF2A_6	75	83	PF00069	0.383
DOC_MAPK_MEF2A_6	86	95	PF00069	0.537
DOC_MAPK_RevD_3	359	374	PF00069	0.240
DOC_PP1_MyPhoNE_1	25	32	PF00149	0.561
DOC_PP1_RVXF_1	322	328	PF00149	0.354
DOC_PP4_FxxP_1	208	211	PF00568	0.463
DOC_USP7_MATH_1	236	240	PF00917	0.265
DOC_USP7_MATH_1	285	289	PF00917	0.574
DOC_USP7_MATH_1	482	486	PF00917	0.457
DOC_USP7_MATH_1	500	504	PF00917	0.507
DOC_WW_Pin1_4	361	366	PF00397	0.331
DOC_WW_Pin1_4	477	482	PF00397	0.341
DOC_WW_Pin1_4	7	12	PF00397	0.428
LIG_14-3-3_CanoR_1	121	127	PF00244	0.368
LIG_14-3-3_CanoR_1	139	147	PF00244	0.432
LIG_14-3-3_CanoR_1	235	241	PF00244	0.410
LIG_14-3-3_CanoR_1	294	298	PF00244	0.668
LIG_14-3-3_CanoR_1	400	405	PF00244	0.298
LIG_14-3-3_CanoR_1	512	517	PF00244	0.431
LIG_Actin_WH2_2	538	554	PF00022	0.464
LIG_APCC_ABBA_1	29	34	PF00400	0.334
LIG_BIR_II_1	1	5	PF00653	0.528
LIG_BRCT_BRCA1_1	417	421	PF00533	0.447
LIG_eIF4E_1	9	15	PF01652	0.409
LIG_FHA_1	121	127	PF00498	0.379
LIG_FHA_1	276	282	PF00498	0.642
LIG_FHA_1	306	312	PF00498	0.399
LIG_FHA_1	366	372	PF00498	0.447
LIG_FHA_1	393	399	PF00498	0.449
LIG_FHA_1	46	52	PF00498	0.550
LIG_FHA_1	464	470	PF00498	0.410
LIG_FHA_1	521	527	PF00498	0.454
LIG_FHA_1	62	68	PF00498	0.524
LIG_FHA_1	85	91	PF00498	0.333
LIG_FHA_2	199	205	PF00498	0.603
LIG_FHA_2	432	438	PF00498	0.520
LIG_FHA_2	466	472	PF00498	0.388
LIG_FHA_2	515	521	PF00498	0.626
LIG_LIR_Apic_2	205	211	PF02991	0.553
LIG_LIR_Apic_2	496	500	PF02991	0.398
LIG_LIR_Gen_1	122	131	PF02991	0.480
LIG_LIR_Gen_1	474	482	PF02991	0.402
LIG_LIR_Gen_1	486	493	PF02991	0.362
LIG_LIR_Nem_3	122	127	PF02991	0.530
LIG_LIR_Nem_3	470	475	PF02991	0.337
LIG_LIR_Nem_3	486	492	PF02991	0.447
LIG_NRBOX	358	364	PF00104	0.287
LIG_PCNA_yPIPBox_3	45	56	PF02747	0.374
LIG_Pex14_2	452	456	PF04695	0.324
LIG_SH2_CRK	3	7	PF00017	0.418
LIG_SH2_CRK	408	412	PF00017	0.476
LIG_SH2_GRB2like	3	6	PF00017	0.495
LIG_SH2_NCK_1	181	185	PF00017	0.531
LIG_SH2_NCK_1	3	7	PF00017	0.484
LIG_SH2_NCK_1	476	480	PF00017	0.316
LIG_SH2_SRC	476	479	PF00017	0.383
LIG_SH2_STAP1	181	185	PF00017	0.325
LIG_SH2_STAP1	250	254	PF00017	0.558
LIG_SH2_STAT3	32	35	PF00017	0.513
LIG_SH2_STAT3	406	409	PF00017	0.483
LIG_SH2_STAT5	108	111	PF00017	0.491
LIG_SH2_STAT5	165	168	PF00017	0.324
LIG_SH2_STAT5	250	253	PF00017	0.552
LIG_SH2_STAT5	254	257	PF00017	0.612
LIG_SH2_STAT5	271	274	PF00017	0.397
LIG_SH2_STAT5	505	508	PF00017	0.553
LIG_SH2_STAT5	80	83	PF00017	0.424
LIG_SH3_3	260	266	PF00018	0.526
LIG_SH3_3	523	529	PF00018	0.595
LIG_SUMO_SIM_anti_2	216	223	PF11976	0.412
LIG_SUMO_SIM_par_1	216	223	PF11976	0.424
LIG_SUMO_SIM_par_1	465	471	PF11976	0.407
LIG_SUMO_SIM_par_1	512	517	PF11976	0.517
LIG_SUMO_SIM_par_1	89	94	PF11976	0.357
LIG_TRAF2_1	38	41	PF00917	0.374
LIG_TRAF2_1	481	484	PF00917	0.460
MOD_CK1_1	102	108	PF00069	0.604
MOD_CK1_1	122	128	PF00069	0.220
MOD_CK1_1	168	174	PF00069	0.517
MOD_CK1_1	293	299	PF00069	0.680
MOD_CK2_1	361	367	PF00069	0.474
MOD_CK2_1	465	471	PF00069	0.459
MOD_CK2_1	477	483	PF00069	0.361
MOD_CK2_1	55	61	PF00069	0.537
MOD_GlcNHglycan	110	113	PF01048	0.543
MOD_GlcNHglycan	158	161	PF01048	0.429
MOD_GlcNHglycan	171	174	PF01048	0.426
MOD_GlcNHglycan	272	275	PF01048	0.455
MOD_GlcNHglycan	502	505	PF01048	0.530
MOD_GlcNHglycan	506	509	PF01048	0.539
MOD_GSK3_1	1	8	PF00069	0.485
MOD_GSK3_1	102	109	PF00069	0.519
MOD_GSK3_1	165	172	PF00069	0.438
MOD_GSK3_1	194	201	PF00069	0.536
MOD_GSK3_1	244	251	PF00069	0.375
MOD_GSK3_1	286	293	PF00069	0.631
MOD_GSK3_1	361	368	PF00069	0.265
MOD_GSK3_1	500	507	PF00069	0.465
MOD_GSK3_1	51	58	PF00069	0.498
MOD_N-GLC_1	133	138	PF02516	0.557
MOD_N-GLC_1	139	144	PF02516	0.568
MOD_N-GLC_1	156	161	PF02516	0.343
MOD_N-GLC_1	365	370	PF02516	0.167
MOD_N-GLC_2	187	189	PF02516	0.414
MOD_NEK2_1	1	6	PF00069	0.558
MOD_NEK2_1	110	115	PF00069	0.437
MOD_NEK2_1	194	199	PF00069	0.397
MOD_NEK2_1	220	225	PF00069	0.424
MOD_NEK2_1	270	275	PF00069	0.484
MOD_NEK2_1	299	304	PF00069	0.529
MOD_NEK2_1	375	380	PF00069	0.353
MOD_NEK2_1	392	397	PF00069	0.413
MOD_NEK2_1	493	498	PF00069	0.454
MOD_NEK2_1	55	60	PF00069	0.545
MOD_NEK2_1	557	562	PF00069	0.460
MOD_NEK2_2	522	527	PF00069	0.646
MOD_PIKK_1	100	106	PF00454	0.602
MOD_PIKK_1	533	539	PF00454	0.547
MOD_PIKK_1	55	61	PF00454	0.584
MOD_PIKK_1	84	90	PF00454	0.401
MOD_PK_1	328	334	PF00069	0.463
MOD_PKA_2	120	126	PF00069	0.367
MOD_PKA_2	138	144	PF00069	0.459
MOD_PKA_2	285	291	PF00069	0.740
MOD_PKA_2	293	299	PF00069	0.590
MOD_PKA_2	392	398	PF00069	0.494
MOD_PKA_2	415	421	PF00069	0.458
MOD_PKB_1	284	292	PF00069	0.583
MOD_Plk_1	133	139	PF00069	0.566
MOD_Plk_1	555	561	PF00069	0.437
MOD_Plk_4	293	299	PF00069	0.657
MOD_Plk_4	400	406	PF00069	0.252
MOD_Plk_4	423	429	PF00069	0.421
MOD_Plk_4	528	534	PF00069	0.534
MOD_Plk_4	62	68	PF00069	0.526
MOD_ProDKin_1	361	367	PF00069	0.331
MOD_ProDKin_1	477	483	PF00069	0.342
MOD_ProDKin_1	7	13	PF00069	0.423
MOD_SUMO_for_1	264	267	PF00179	0.440
MOD_SUMO_rev_2	414	424	PF00179	0.442
MOD_SUMO_rev_2	535	542	PF00179	0.433
TRG_DiLeu_BaEn_1	367	372	PF01217	0.428
TRG_DiLeu_BaEn_1	509	514	PF01217	0.447
TRG_DiLeu_BaLyEn_6	541	546	PF01217	0.523
TRG_DiLeu_LyEn_5	509	514	PF01217	0.447
TRG_ENDOCYTIC_2	3	6	PF00928	0.570
TRG_ENDOCYTIC_2	408	411	PF00928	0.423
TRG_ENDOCYTIC_2	476	479	PF00928	0.318
TRG_ENDOCYTIC_2	80	83	PF00928	0.511
TRG_ER_diArg_1	150	153	PF00400	0.423
TRG_ER_diArg_1	283	286	PF00400	0.761
TRG_ER_diArg_1	342	345	PF00400	0.560
TRG_NES_CRM1_1	387	402	PF08389	0.409
TRG_Pf-PMV_PEXEL_1	544	548	PF00026	0.473

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HW03	Leptomonas seymouri	62%	98%
A0A0S4JII0	Bodo saltans	29%	97%
A0A1X0P3R1	Trypanosomatidae	44%	100%
A0A3Q8IJI1	Leishmania donovani	95%	99%
A0A422NKK1	Trypanosoma rangeli	44%	100%
A4HPH8	Leishmania braziliensis	85%	100%
A4IDA0	Leishmania infantum	95%	99%
D0A8E0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	85%
Q4Q1A7	Leishmania major	94%	100%
V5AU55	Trypanosoma cruzi	42%	100%