LeishMANIAdb
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Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:
Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex
Gene product:
dihydrolipoamide acetyltransferase precursor, putative
Species:
Leishmania mexicana
UniProt:
E9AT43_LEIMU
TriTrypDb:
LmxM.36.2660
Length:
466

Localization

Secreted promastigote
Source Evidence on protein Close homologs
Cuervo et al. no yes: 0
Hassani et al. no yes: 0
Forrest at al. (metacyclic) no yes: 0
Forrest at al. (procyclic) no yes: 0
Silverman et al. no yes: 0
Pissara et al. yes yes: 15
Secreted amastigote
Source Evidence on protein Close homologs
Pires et al. no yes: 0
Exosome
Source Evidence on protein Close homologs
Silverman et al. no yes: 7
Glycosome
Source Evidence on protein Close homologs
Jamdhade et al. no yes: 7
Predictions
Source Evidence on protein Close homologs
DeepLoc
SignalP6 no yes: 2, no: 8
NetGPI no yes: 0, no: 10
Cellular components
Term Name Level Count
GO:0032991 protein-containing complex 1 11
GO:0045254 pyruvate dehydrogenase complex 4 11
GO:1902494 catalytic complex 2 11
GO:1990204 oxidoreductase complex 3 11
GO:0005739 mitochondrion 5 1
GO:0005967 mitochondrial pyruvate dehydrogenase complex 3 1
GO:0020023 kinetoplast 2 1
GO:0043226 organelle 2 2
GO:0043227 membrane-bounded organelle 3 2
GO:0043229 intracellular organelle 3 2
GO:0043231 intracellular membrane-bounded organelle 4 2
GO:0098798 mitochondrial protein-containing complex 2 1
GO:0110165 cellular anatomical entity 1 2

Expansion

Sequence features

E9AT43
Sequence
MSA
Disorder
Secondary
Topology
Domains
SignalP
GPI
Phosphorylations
ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AT43

Function

Biological processes
Term Name Level Count
GO:0006082 organic acid metabolic process 3 11
GO:0006084 acetyl-CoA metabolic process 5 11
GO:0006085 acetyl-CoA biosynthetic process 6 11
GO:0006086 acetyl-CoA biosynthetic process from pyruvate 7 11
GO:0006090 pyruvate metabolic process 7 11
GO:0006139 nucleobase-containing compound metabolic process 3 11
GO:0006163 purine nucleotide metabolic process 5 11
GO:0006164 purine nucleotide biosynthetic process 6 11
GO:0006637 acyl-CoA metabolic process 4 11
GO:0006725 cellular aromatic compound metabolic process 3 11
GO:0006753 nucleoside phosphate metabolic process 4 11
GO:0006790 sulfur compound metabolic process 3 11
GO:0006793 phosphorus metabolic process 3 11
GO:0006796 phosphate-containing compound metabolic process 4 11
GO:0006807 nitrogen compound metabolic process 2 11
GO:0008152 metabolic process 1 11
GO:0009058 biosynthetic process 2 11
GO:0009117 nucleotide metabolic process 5 11
GO:0009150 purine ribonucleotide metabolic process 6 11
GO:0009152 purine ribonucleotide biosynthetic process 7 11
GO:0009165 nucleotide biosynthetic process 6 11
GO:0009259 ribonucleotide metabolic process 5 11
GO:0009260 ribonucleotide biosynthetic process 6 11
GO:0009987 cellular process 1 11
GO:0018130 heterocycle biosynthetic process 4 11
GO:0019438 aromatic compound biosynthetic process 4 11
GO:0019637 organophosphate metabolic process 3 11
GO:0019693 ribose phosphate metabolic process 4 11
GO:0019752 carboxylic acid metabolic process 5 11
GO:0032787 monocarboxylic acid metabolic process 6 11
GO:0033865 nucleoside bisphosphate metabolic process 5 11
GO:0033866 nucleoside bisphosphate biosynthetic process 6 11
GO:0033875 ribonucleoside bisphosphate metabolic process 6 11
GO:0034030 ribonucleoside bisphosphate biosynthetic process 7 11
GO:0034032 purine nucleoside bisphosphate metabolic process 5 11
GO:0034033 purine nucleoside bisphosphate biosynthetic process 6 11
GO:0034641 cellular nitrogen compound metabolic process 3 11
GO:0034654 nucleobase-containing compound biosynthetic process 4 11
GO:0035383 thioester metabolic process 3 11
GO:0035384 thioester biosynthetic process 4 11
GO:0043436 oxoacid metabolic process 4 11
GO:0043603 amide metabolic process 3 11
GO:0043604 amide biosynthetic process 4 11
GO:0044237 cellular metabolic process 2 11
GO:0044238 primary metabolic process 2 11
GO:0044249 cellular biosynthetic process 3 11
GO:0044271 cellular nitrogen compound biosynthetic process 4 11
GO:0044272 sulfur compound biosynthetic process 4 11
GO:0044281 small molecule metabolic process 2 11
GO:0046390 ribose phosphate biosynthetic process 5 11
GO:0046483 heterocycle metabolic process 3 11
GO:0055086 nucleobase-containing small molecule metabolic process 3 11
GO:0071616 acyl-CoA biosynthetic process 5 11
GO:0071704 organic substance metabolic process 2 11
GO:0072521 purine-containing compound metabolic process 4 11
GO:0072522 purine-containing compound biosynthetic process 5 11
GO:0090407 organophosphate biosynthetic process 4 11
GO:1901135 carbohydrate derivative metabolic process 3 11
GO:1901137 carbohydrate derivative biosynthetic process 4 11
GO:1901293 nucleoside phosphate biosynthetic process 5 11
GO:1901360 organic cyclic compound metabolic process 3 11
GO:1901362 organic cyclic compound biosynthetic process 4 11
GO:1901564 organonitrogen compound metabolic process 3 11
GO:1901566 organonitrogen compound biosynthetic process 4 11
GO:1901576 organic substance biosynthetic process 3 11
Molecular functions
Term Name Level Count
GO:0003824 catalytic activity 1 11
GO:0016740 transferase activity 2 11
GO:0016746 acyltransferase activity 3 11
GO:0004742 dihydrolipoyllysine-residue acetyltransferase activity 3 2
GO:0016407 acetyltransferase activity 5 2
GO:0016417 S-acyltransferase activity 5 2
GO:0016418 S-acetyltransferase activity 6 2
GO:0016747 acyltransferase activity, transferring groups other than amino-acyl groups 4 2
GO:0140096 catalytic activity, acting on a protein 2 2

Putative motif mimicry

Leishmania From To Domain/Motif Score
CLV_NRD_NRD_1 154 156 PF00675 0.325
CLV_PCSK_KEX2_1 246 248 PF00082 0.364
CLV_PCSK_PC1ET2_1 246 248 PF00082 0.383
CLV_PCSK_SKI1_1 17 21 PF00082 0.512
CLV_PCSK_SKI1_1 78 82 PF00082 0.489
CLV_PCSK_SKI1_1 9 13 PF00082 0.637
DEG_Kelch_Keap1_1 415 420 PF01344 0.462
DEG_Nend_Nbox_1 1 3 PF02207 0.607
DOC_CKS1_1 328 333 PF01111 0.364
DOC_CKS1_1 334 339 PF01111 0.364
DOC_CYCLIN_yClb3_PxF_3 226 232 PF00134 0.708
DOC_CYCLIN_yCln2_LP_2 213 219 PF00134 0.693
DOC_MAPK_DCC_7 17 26 PF00069 0.493
DOC_MAPK_MEF2A_6 317 326 PF00069 0.348
DOC_PP2B_LxvP_1 213 216 PF13499 0.692
DOC_USP7_MATH_1 186 190 PF00917 0.573
DOC_USP7_MATH_1 191 195 PF00917 0.651
DOC_USP7_MATH_1 402 406 PF00917 0.407
DOC_USP7_MATH_1 63 67 PF00917 0.400
DOC_USP7_UBL2_3 355 359 PF12436 0.364
DOC_WW_Pin1_4 148 153 PF00397 0.500
DOC_WW_Pin1_4 19 24 PF00397 0.453
DOC_WW_Pin1_4 222 227 PF00397 0.639
DOC_WW_Pin1_4 27 32 PF00397 0.326
DOC_WW_Pin1_4 327 332 PF00397 0.364
DOC_WW_Pin1_4 333 338 PF00397 0.364
LIG_14-3-3_CanoR_1 177 183 PF00244 0.591
LIG_14-3-3_CanoR_1 299 304 PF00244 0.409
LIG_14-3-3_CanoR_1 317 323 PF00244 0.329
LIG_14-3-3_CanoR_1 78 88 PF00244 0.392
LIG_APCC_ABBAyCdc20_2 100 106 PF00400 0.614
LIG_FHA_1 299 305 PF00498 0.217
LIG_FHA_1 319 325 PF00498 0.363
LIG_FHA_1 330 336 PF00498 0.345
LIG_FHA_1 352 358 PF00498 0.423
LIG_FHA_1 373 379 PF00498 0.364
LIG_FHA_1 388 394 PF00498 0.364
LIG_FHA_1 422 428 PF00498 0.476
LIG_FHA_1 90 96 PF00498 0.407
LIG_FHA_2 177 183 PF00498 0.563
LIG_FHA_2 286 292 PF00498 0.373
LIG_LIR_Gen_1 15 26 PF02991 0.465
LIG_LIR_Gen_1 53 63 PF02991 0.365
LIG_LIR_Gen_1 73 83 PF02991 0.178
LIG_LIR_Nem_3 15 21 PF02991 0.448
LIG_LIR_Nem_3 53 58 PF02991 0.365
LIG_LIR_Nem_3 73 79 PF02991 0.178
LIG_MYND_1 27 31 PF01753 0.364
LIG_PDZ_Class_2 461 466 PF00595 0.364
LIG_Pex14_1 311 315 PF04695 0.407
LIG_Pex14_1 450 454 PF04695 0.364
LIG_Pex14_2 433 437 PF04695 0.364
LIG_REV1ctd_RIR_1 73 82 PF16727 0.476
LIG_SH2_GRB2like 67 70 PF00017 0.383
LIG_SH2_NCK_1 319 323 PF00017 0.407
LIG_SH2_NCK_1 67 71 PF00017 0.383
LIG_SH2_SRC 67 70 PF00017 0.383
LIG_SH2_STAP1 153 157 PF00017 0.591
LIG_SH2_STAT5 260 263 PF00017 0.364
LIG_SH2_STAT5 292 295 PF00017 0.353
LIG_SH3_3 124 130 PF00018 0.741
LIG_SH3_3 195 201 PF00018 0.742
LIG_SH3_3 213 219 PF00018 0.665
LIG_SH3_3 224 230 PF00018 0.595
LIG_SH3_3 325 331 PF00018 0.364
LIG_SUMO_SIM_par_1 320 325 PF11976 0.383
LIG_SUMO_SIM_par_1 346 351 PF11976 0.437
LIG_SUMO_SIM_par_1 399 405 PF11976 0.352
LIG_SxIP_EBH_1 17 30 PF03271 0.383
LIG_SxIP_EBH_1 228 241 PF03271 0.453
LIG_TRAF2_1 122 125 PF00917 0.780
LIG_TRAF2_1 70 73 PF00917 0.425
LIG_TRAF2_1 83 86 PF00917 0.366
LIG_UBA3_1 270 275 PF00899 0.407
MOD_CDK_SPxxK_3 148 155 PF00069 0.511
MOD_CDK_SPxxK_3 333 340 PF00069 0.364
MOD_CK1_1 351 357 PF00069 0.462
MOD_CK2_1 79 85 PF00069 0.478
MOD_GlcNHglycan 143 146 PF01048 0.666
MOD_GlcNHglycan 165 168 PF01048 0.345
MOD_GlcNHglycan 188 191 PF01048 0.637
MOD_GlcNHglycan 193 196 PF01048 0.710
MOD_GlcNHglycan 404 407 PF01048 0.407
MOD_GlcNHglycan 81 84 PF01048 0.515
MOD_GSK3_1 186 193 PF00069 0.633
MOD_GSK3_1 235 242 PF00069 0.414
MOD_GSK3_1 318 325 PF00069 0.385
MOD_GSK3_1 329 336 PF00069 0.343
MOD_GSK3_1 362 369 PF00069 0.407
MOD_GSK3_1 372 379 PF00069 0.322
MOD_GSK3_1 5 12 PF00069 0.644
MOD_N-GLC_1 230 235 PF02516 0.371
MOD_N-GLC_1 307 312 PF02516 0.364
MOD_N-GLC_1 68 73 PF02516 0.383
MOD_NEK2_1 248 253 PF00069 0.375
MOD_NEK2_1 381 386 PF00069 0.375
MOD_NEK2_1 421 426 PF00069 0.455
MOD_NEK2_2 236 241 PF00069 0.437
MOD_PKA_2 176 182 PF00069 0.558
MOD_PKA_2 298 304 PF00069 0.318
MOD_PKB_1 3 11 PF00069 0.671
MOD_Plk_1 307 313 PF00069 0.380
MOD_Plk_1 322 328 PF00069 0.364
MOD_Plk_1 84 90 PF00069 0.409
MOD_Plk_2-3 68 74 PF00069 0.383
MOD_Plk_4 299 305 PF00069 0.434
MOD_Plk_4 460 466 PF00069 0.364
MOD_Plk_4 84 90 PF00069 0.453
MOD_ProDKin_1 148 154 PF00069 0.500
MOD_ProDKin_1 19 25 PF00069 0.455
MOD_ProDKin_1 222 228 PF00069 0.637
MOD_ProDKin_1 27 33 PF00069 0.326
MOD_ProDKin_1 327 333 PF00069 0.364
MOD_SUMO_rev_2 349 357 PF00179 0.364
TRG_ENDOCYTIC_2 259 262 PF00928 0.348
TRG_ENDOCYTIC_2 319 322 PF00928 0.364
TRG_ER_diArg_1 2 5 PF00400 0.603
TRG_Pf-PMV_PEXEL_1 345 349 PF00026 0.364

Homologs

Protein Taxonomy Sequence identity Coverage
A0A0N1IHV8 Leptomonas seymouri 81% 100%
A0A0N1PBB0 Leptomonas seymouri 28% 82%
A0A0S4JC61 Bodo saltans 59% 99%
A0A0S4JS90 Bodo saltans 27% 100%
A0A1X0NKN9 Trypanosomatidae 64% 99%
A0A1X0P2L7 Trypanosomatidae 23% 93%
A0A3Q8IJE7 Leishmania donovani 93% 100%
A0A3S5H5G3 Leishmania donovani 26% 98%
A0A3S5ISA9 Trypanosoma rangeli 23% 100%
A4H464 Leishmania braziliensis 26% 99%
A4HPD2 Leishmania braziliensis 84% 99%
A4ICH2 Leishmania infantum 93% 100%
C9ZPW7 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 23% 100%
D0A3J2 Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972) 61% 100%
E9AG63 Leishmania infantum 25% 98%
E9AKC2 Leishmania mexicana (strain MHOM/GT/2001/U1103) 27% 98%
G0S4X6 Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) 42% 100%
O00330 Homo sapiens 36% 93%
O31550 Bacillus subtilis (strain 168) 30% 100%
O59816 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 41% 96%
O66119 Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) 35% 100%
O94681 Schizosaccharomyces pombe (strain 972 / ATCC 24843) 26% 100%
P06959 Escherichia coli (strain K12) 33% 74%
P08461 Rattus norvegicus 44% 74%
P09062 Pseudomonas putida 28% 100%
P0AFG6 Escherichia coli (strain K12) 30% 100%
P0AFG7 Escherichia coli O157:H7 30% 100%
P10515 Homo sapiens 42% 72%
P10802 Azotobacter vinelandii 29% 73%
P11181 Bos taurus 25% 97%
P11182 Homo sapiens 25% 97%
P11961 Geobacillus stearothermophilus 31% 100%
P12695 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 38% 97%
P16263 Bacillus subtilis (strain 168) 31% 100%
P19262 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) 29% 100%
P20285 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) 39% 100%
P20708 Azotobacter vinelandii 29% 100%
P21883 Bacillus subtilis (strain 168) 32% 100%
P22439 Bos taurus 38% 93%
P35489 Acholeplasma laidlawii 29% 86%
P36413 Dictyostelium discoideum 39% 73%
P37942 Bacillus subtilis (strain 168) 33% 100%
P45118 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) 32% 82%
P45302 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) 28% 100%
P47514 Mycoplasma genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 / G37) 24% 100%
P52993 Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) 32% 100%
P53395 Mus musculus 26% 97%
P57302 Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) 23% 100%
P57389 Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) 23% 100%
P65635 Staphylococcus aureus (strain Mu50 / ATCC 700699) 32% 100%
P65636 Staphylococcus aureus (strain N315) 32% 100%
Q0WQF7 Arabidopsis thaliana 37% 73%
Q19749 Caenorhabditis elegans 42% 92%
Q1RHI5 Rickettsia bellii (strain RML369-C) 29% 100%
Q1RJT3 Rickettsia bellii (strain RML369-C) 38% 100%
Q23571 Caenorhabditis elegans 23% 100%
Q2FH26 Staphylococcus aureus (strain USA300) 29% 100%
Q2FYM2 Staphylococcus aureus (strain NCTC 8325 / PS 47) 29% 100%
Q2YY06 Staphylococcus aureus (strain bovine RF122 / ET3-1) 29% 100%
Q49110 Mycoplasma capricolum subsp. capricolum (strain California kid / ATCC 27343 / NCTC 10154) 29% 100%
Q49XM4 Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) 29% 100%
Q4L6C3 Staphylococcus haemolyticus (strain JCSC1435) 27% 100%
Q4Q1F5 Leishmania major 93% 99%
Q4QJI5 Leishmania major 25% 98%
Q4UKI7 Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) 29% 100%
Q4ULG1 Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) 34% 100%
Q59098 Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337) 31% 84%
Q59638 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 30% 85%
Q59821 Staphylococcus aureus 32% 100%
Q5HG07 Staphylococcus aureus (strain COL) 29% 100%
Q5HGY9 Staphylococcus aureus (strain COL) 32% 100%
Q5HPC7 Staphylococcus epidermidis (strain ATCC 35984 / RP62A) 27% 100%
Q5HQ74 Staphylococcus epidermidis (strain ATCC 35984 / RP62A) 30% 100%
Q5M729 Arabidopsis thaliana 41% 86%
Q68WK6 Rickettsia typhi (strain ATCC VR-144 / Wilmington) 33% 100%
Q68XI8 Rickettsia typhi (strain ATCC VR-144 / Wilmington) 29% 100%
Q6ABX9 Leifsonia xyli subsp. xyli (strain CTCB07) 25% 100%
Q6FYD4 Bartonella quintana (strain Toulouse) 29% 100%
Q6G9E9 Staphylococcus aureus (strain MSSA476) 29% 100%
Q6GAB9 Staphylococcus aureus (strain MSSA476) 32% 100%
Q6GGZ6 Staphylococcus aureus (strain MRSA252) 29% 100%
Q6GHZ0 Staphylococcus aureus (strain MRSA252) 32% 100%
Q7A5N4 Staphylococcus aureus (strain N315) 29% 100%
Q89AJ6 Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) 23% 100%
Q89AQ9 Buchnera aphidicola subsp. Baizongia pistaciae (strain Bp) 21% 100%
Q8BKZ9 Mus musculus 36% 93%
Q8BMF4 Mus musculus 43% 73%
Q8CSL9 Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) 27% 100%
Q8CT13 Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) 29% 100%
Q8GCY1 Bartonella vinsonii subsp. berkhoffii 27% 100%
Q8K9T8 Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) 25% 100%
Q8NNJ2 Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) 30% 69%
Q8NWR7 Staphylococcus aureus (strain MW2) 29% 100%
Q8NX76 Staphylococcus aureus (strain MW2) 32% 100%
Q8RWN9 Arabidopsis thaliana 40% 86%
Q92HK7 Rickettsia conorii (strain ATCC VR-613 / Malish 7) 34% 100%
Q92J43 Rickettsia conorii (strain ATCC VR-613 / Malish 7) 29% 100%
Q99U75 Staphylococcus aureus (strain Mu50 / ATCC 700699) 29% 100%
Q9C8P0 Arabidopsis thaliana 31% 100%
Q9I1M0 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 26% 100%
Q9I3D2 Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) 31% 100%
Q9M7Z1 Arabidopsis thaliana 23% 96%
Q9R9N3 Rhizobium meliloti (strain 1021) 40% 100%
Q9SQI8 Arabidopsis thaliana 32% 97%
Q9ZD20 Rickettsia prowazekii (strain Madrid E) 33% 100%
Q9ZDY4 Rickettsia prowazekii (strain Madrid E) 28% 100%
V5BFP9 Trypanosoma cruzi 63% 99%
V5BJF4 Trypanosoma cruzi 25% 100%

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LeishMANIAdb - Server version: v0.0.2. - Database version: v0.0.1. - ChangeLog - © 2022-2025 Protein Bioinformatics Research Group, Institute of Enzymology, RCNS