Folylpolyglutamate synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

folylpolyglutamate synthetase

Species:

Leishmania mexicana

UniProt:

E9AT38_LEIMU

TriTrypDb:

LmxM.36.2610

Length:

537

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AT38

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AT38

Function

Biological processes
Term	Name	Level	Count
GO:0006575	cellular modified amino acid metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006760	folic acid-containing compound metabolic process	4	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009396	folic acid-containing compound biosynthetic process	5	1
GO:0009987	cellular process	1	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0042398	cellular modified amino acid biosynthetic process	4	1
GO:0042558	pteridine-containing compound metabolic process	4	1
GO:0042559	pteridine-containing compound biosynthetic process	5	1
GO:0044237	cellular metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046900	tetrahydrofolylpolyglutamate metabolic process	5	1
GO:0046901	tetrahydrofolylpolyglutamate biosynthetic process	6	1
GO:0071704	organic substance metabolic process	2	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1901566	organonitrogen compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004326	tetrahydrofolylpolyglutamate synthase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	148	152	PF00656	0.391
CLV_C14_Caspase3-7	448	452	PF00656	0.361
CLV_C14_Caspase3-7	62	66	PF00656	0.492
CLV_NRD_NRD_1	287	289	PF00675	0.446
CLV_NRD_NRD_1	329	331	PF00675	0.343
CLV_NRD_NRD_1	402	404	PF00675	0.314
CLV_NRD_NRD_1	534	536	PF00675	0.532
CLV_NRD_NRD_1	58	60	PF00675	0.598
CLV_NRD_NRD_1	76	78	PF00675	0.657
CLV_PCSK_KEX2_1	329	331	PF00082	0.343
CLV_PCSK_KEX2_1	402	404	PF00082	0.322
CLV_PCSK_KEX2_1	414	416	PF00082	0.342
CLV_PCSK_KEX2_1	534	536	PF00082	0.534
CLV_PCSK_KEX2_1	57	59	PF00082	0.582
CLV_PCSK_KEX2_1	76	78	PF00082	0.612
CLV_PCSK_KEX2_1	89	91	PF00082	0.512
CLV_PCSK_PC1ET2_1	414	416	PF00082	0.452
CLV_PCSK_PC1ET2_1	57	59	PF00082	0.634
CLV_PCSK_PC1ET2_1	89	91	PF00082	0.411
CLV_PCSK_PC7_1	72	78	PF00082	0.464
CLV_PCSK_SKI1_1	195	199	PF00082	0.322
CLV_PCSK_SKI1_1	280	284	PF00082	0.371
CLV_PCSK_SKI1_1	299	303	PF00082	0.320
CLV_PCSK_SKI1_1	322	326	PF00082	0.278
CLV_PCSK_SKI1_1	345	349	PF00082	0.325
CLV_PCSK_SKI1_1	402	406	PF00082	0.362
CLV_PCSK_SKI1_1	414	418	PF00082	0.324
CLV_PCSK_SKI1_1	473	477	PF00082	0.317
CLV_PCSK_SKI1_1	524	528	PF00082	0.344
CLV_PCSK_SKI1_1	61	65	PF00082	0.540
DEG_SCF_TRCP1_1	14	20	PF00400	0.479
DOC_ANK_TNKS_1	146	153	PF00023	0.433
DOC_CDC14_PxL_1	95	103	PF14671	0.386
DOC_CKS1_1	290	295	PF01111	0.384
DOC_CYCLIN_RxL_1	397	410	PF00134	0.365
DOC_MAPK_DCC_7	330	340	PF00069	0.385
DOC_MAPK_gen_1	288	296	PF00069	0.448
DOC_MAPK_gen_1	61	71	PF00069	0.567
DOC_MAPK_gen_1	89	97	PF00069	0.471
DOC_MAPK_MEF2A_6	195	204	PF00069	0.352
DOC_MAPK_MEF2A_6	288	297	PF00069	0.355
DOC_MAPK_MEF2A_6	64	73	PF00069	0.371
DOC_PP1_RVXF_1	101	107	PF00149	0.278
DOC_PP1_RVXF_1	401	408	PF00149	0.313
DOC_PP1_RVXF_1	92	98	PF00149	0.376
DOC_PP2B_LxvP_1	493	496	PF13499	0.475
DOC_PP4_FxxP_1	136	139	PF00568	0.294
DOC_PP4_FxxP_1	269	272	PF00568	0.400
DOC_USP7_MATH_1	12	16	PF00917	0.619
DOC_USP7_MATH_1	186	190	PF00917	0.464
DOC_USP7_MATH_1	303	307	PF00917	0.475
DOC_USP7_MATH_1	354	358	PF00917	0.373
DOC_USP7_MATH_1	433	437	PF00917	0.511
DOC_USP7_MATH_1	445	449	PF00917	0.573
DOC_USP7_MATH_1	496	500	PF00917	0.510
DOC_USP7_MATH_1	53	57	PF00917	0.523
DOC_USP7_UBL2_3	285	289	PF12436	0.369
DOC_USP7_UBL2_3	414	418	PF12436	0.466
DOC_WW_Pin1_4	135	140	PF00397	0.296
DOC_WW_Pin1_4	17	22	PF00397	0.737
DOC_WW_Pin1_4	184	189	PF00397	0.523
DOC_WW_Pin1_4	289	294	PF00397	0.335
LIG_14-3-3_CanoR_1	439	447	PF00244	0.580
LIG_14-3-3_CanoR_1	453	461	PF00244	0.451
LIG_14-3-3_CanoR_1	482	491	PF00244	0.504
LIG_14-3-3_CanoR_1	58	64	PF00244	0.478
LIG_BIR_III_4	449	453	PF00653	0.347
LIG_Clathr_ClatBox_1	294	298	PF01394	0.399
LIG_DLG_GKlike_1	59	67	PF00625	0.396
LIG_FHA_1	120	126	PF00498	0.255
LIG_FHA_1	224	230	PF00498	0.290
LIG_FHA_1	277	283	PF00498	0.367
LIG_FHA_1	290	296	PF00498	0.414
LIG_FHA_1	367	373	PF00498	0.351
LIG_FHA_1	50	56	PF00498	0.447
LIG_FHA_2	146	152	PF00498	0.399
LIG_FHA_2	248	254	PF00498	0.283
LIG_FHA_2	407	413	PF00498	0.408
LIG_HP1_1	293	297	PF01393	0.378
LIG_LIR_Gen_1	169	176	PF02991	0.365
LIG_LIR_Gen_1	238	247	PF02991	0.321
LIG_LIR_Gen_1	385	394	PF02991	0.404
LIG_LIR_Gen_1	483	491	PF02991	0.234
LIG_LIR_Nem_3	157	163	PF02991	0.311
LIG_LIR_Nem_3	169	174	PF02991	0.396
LIG_LIR_Nem_3	306	310	PF02991	0.388
LIG_LIR_Nem_3	385	391	PF02991	0.393
LIG_LIR_Nem_3	483	487	PF02991	0.327
LIG_LIR_Nem_3	515	519	PF02991	0.329
LIG_Pex14_1	468	472	PF04695	0.340
LIG_Pex14_2	194	198	PF04695	0.270
LIG_REV1ctd_RIR_1	191	199	PF16727	0.346
LIG_SH2_SRC	371	374	PF00017	0.343
LIG_SH2_SRC	423	426	PF00017	0.254
LIG_SH2_STAP1	121	125	PF00017	0.257
LIG_SH2_STAP1	160	164	PF00017	0.418
LIG_SH2_STAT5	121	124	PF00017	0.283
LIG_SH2_STAT5	163	166	PF00017	0.285
LIG_SH2_STAT5	203	206	PF00017	0.354
LIG_SH2_STAT5	371	374	PF00017	0.299
LIG_SH2_STAT5	408	411	PF00017	0.385
LIG_SH2_STAT5	423	426	PF00017	0.365
LIG_SH3_3	19	25	PF00018	0.593
LIG_SH3_3	271	277	PF00018	0.504
LIG_SH3_3	333	339	PF00018	0.384
LIG_SH3_3	419	425	PF00018	0.254
LIG_SH3_3	432	438	PF00018	0.332
LIG_SUMO_SIM_anti_2	211	216	PF11976	0.243
LIG_SUMO_SIM_anti_2	231	238	PF11976	0.287
LIG_SUMO_SIM_anti_2	292	298	PF11976	0.463
LIG_SUMO_SIM_anti_2	319	327	PF11976	0.299
LIG_SUMO_SIM_anti_2	488	495	PF11976	0.399
LIG_SUMO_SIM_par_1	292	298	PF11976	0.391
LIG_TRAF2_1	250	253	PF00917	0.249
LIG_UBA3_1	301	309	PF00899	0.355
LIG_UBA3_1	337	345	PF00899	0.482
MOD_CDC14_SPxK_1	187	190	PF00782	0.526
MOD_CDK_SPxK_1	184	190	PF00069	0.510
MOD_CK1_1	119	125	PF00069	0.350
MOD_CK1_1	225	231	PF00069	0.362
MOD_CK1_1	234	240	PF00069	0.313
MOD_CK1_1	380	386	PF00069	0.459
MOD_CK2_1	247	253	PF00069	0.249
MOD_CK2_1	482	488	PF00069	0.342
MOD_CK2_1	496	502	PF00069	0.352
MOD_Cter_Amidation	532	535	PF01082	0.514
MOD_Cter_Amidation	74	77	PF01082	0.526
MOD_GlcNHglycan	118	121	PF01048	0.368
MOD_GlcNHglycan	14	17	PF01048	0.743
MOD_GlcNHglycan	382	385	PF01048	0.423
MOD_GlcNHglycan	390	394	PF01048	0.436
MOD_GlcNHglycan	443	446	PF01048	0.677
MOD_GlcNHglycan	484	487	PF01048	0.490
MOD_GlcNHglycan	49	52	PF01048	0.688
MOD_GSK3_1	115	122	PF00069	0.270
MOD_GSK3_1	135	142	PF00069	0.471
MOD_GSK3_1	231	238	PF00069	0.335
MOD_GSK3_1	343	350	PF00069	0.429
MOD_GSK3_1	389	396	PF00069	0.388
MOD_GSK3_1	441	448	PF00069	0.544
MOD_GSK3_1	45	52	PF00069	0.487
MOD_N-GLC_1	366	371	PF02516	0.390
MOD_N-GLC_2	79	81	PF02516	0.632
MOD_NEK2_1	198	203	PF00069	0.285
MOD_NEK2_1	247	252	PF00069	0.392
MOD_NEK2_1	389	394	PF00069	0.401
MOD_NEK2_1	85	90	PF00069	0.359
MOD_NEK2_2	2	7	PF00069	0.756
MOD_NEK2_2	366	371	PF00069	0.366
MOD_PIKK_1	176	182	PF00454	0.407
MOD_PIKK_1	354	360	PF00454	0.338
MOD_PIKK_1	382	388	PF00454	0.445
MOD_PKA_2	438	444	PF00069	0.559
MOD_PKA_2	452	458	PF00069	0.507
MOD_PKA_2	71	77	PF00069	0.534
MOD_Plk_1	145	151	PF00069	0.490
MOD_Plk_1	156	162	PF00069	0.404
MOD_Plk_1	231	237	PF00069	0.283
MOD_Plk_1	343	349	PF00069	0.393
MOD_Plk_1	366	372	PF00069	0.444
MOD_Plk_1	473	479	PF00069	0.396
MOD_Plk_4	198	204	PF00069	0.308
MOD_Plk_4	231	237	PF00069	0.283
MOD_Plk_4	343	349	PF00069	0.439
MOD_Plk_4	512	518	PF00069	0.318
MOD_ProDKin_1	135	141	PF00069	0.297
MOD_ProDKin_1	17	23	PF00069	0.738
MOD_ProDKin_1	184	190	PF00069	0.529
MOD_ProDKin_1	289	295	PF00069	0.332
MOD_SUMO_rev_2	319	324	PF00179	0.326
MOD_SUMO_rev_2	410	416	PF00179	0.475
TRG_DiLeu_BaEn_2	101	107	PF01217	0.397
TRG_DiLeu_BaLyEn_6	150	155	PF01217	0.403
TRG_DiLeu_BaLyEn_6	521	526	PF01217	0.184
TRG_ENDOCYTIC_2	171	174	PF00928	0.428
TRG_ENDOCYTIC_2	241	244	PF00928	0.392
TRG_ENDOCYTIC_2	472	475	PF00928	0.366
TRG_ER_diArg_1	103	106	PF00400	0.369
TRG_ER_diArg_1	30	33	PF00400	0.559
TRG_ER_diArg_1	329	331	PF00400	0.343
TRG_ER_diArg_1	401	403	PF00400	0.346
TRG_ER_diArg_1	534	536	PF00400	0.459
TRG_ER_diArg_1	58	61	PF00400	0.568
TRG_ER_diArg_1	76	78	PF00400	0.558
TRG_NLS_MonoExtC_3	56	61	PF00514	0.492
TRG_Pf-PMV_PEXEL_1	153	157	PF00026	0.435

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6C4	Leptomonas seymouri	74%	82%
A0A0S4KJ93	Bodo saltans	47%	100%
A0A1X0NM42	Trypanosomatidae	56%	100%
A0A3Q8IJ59	Leishmania donovani	91%	99%
A0A3R7RIQ8	Trypanosoma rangeli	54%	100%
A4HPC7	Leishmania braziliensis	84%	99%
A4ICG7	Leishmania infantum	91%	99%
A6H751	Bos taurus	31%	92%
A6ZP80	Saccharomyces cerevisiae (strain YJM789)	30%	98%
B3LJR0	Saccharomyces cerevisiae (strain RM11-1a)	30%	98%
B5VSC3	Saccharomyces cerevisiae (strain AWRI1631)	30%	98%
C8ZGZ3	Saccharomyces cerevisiae (strain Lalvin EC1118 / Prise de mousse)	30%	98%
D0A3I6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
E7KIA3	Saccharomyces cerevisiae (strain AWRI796)	30%	98%
E7KUJ4	Saccharomyces cerevisiae (strain Lalvin QA23)	30%	98%
E7NMM0	Saccharomyces cerevisiae (strain FostersO)	30%	98%
E7Q9C7	Saccharomyces cerevisiae (strain FostersB)	30%	98%
E7QKX4	Saccharomyces cerevisiae (strain Zymaflore VL3)	30%	98%
F4J2K2	Arabidopsis thaliana	30%	86%
F4JYE9	Arabidopsis thaliana	26%	100%
F4K2A1	Arabidopsis thaliana	29%	94%
O13492	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	29%	100%
O74742	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P48760	Mus musculus	31%	91%
Q05932	Homo sapiens	32%	91%
Q08645	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	98%
Q09509	Caenorhabditis elegans	29%	100%
Q12676	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	23%	100%
Q4Q1G0	Leishmania major	91%	100%
Q54CY5	Dictyostelium discoideum	29%	86%
Q8W035	Arabidopsis thaliana	31%	100%
Q924L9	Cricetulus griseus	30%	91%
Q9UTD0	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
Q9Y893	Candida albicans	30%	100%
V5BKB0	Trypanosoma cruzi	54%	100%