Putative DEAD/DEAH box helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ATP-dependent DEAD/H RNA helicase, putative

Species:

Leishmania mexicana

UniProt:

E9AT30_LEIMU

TriTrypDb:

LmxM.36.2530

Length:

704

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 25
NetGPI	no	yes: 0, no: 25

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005737	cytoplasm	2	3
GO:0010494	cytoplasmic stress granule	5	1
GO:0034399	nuclear periphery	2	1
GO:0035770	ribonucleoprotein granule	3	1
GO:0036464	cytoplasmic ribonucleoprotein granule	4	1
GO:0043226	organelle	2	3
GO:0043227	membrane-bounded organelle	3	3
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	3
GO:0043231	intracellular membrane-bounded organelle	4	3
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0097165	nuclear stress granule	4	1
GO:0099080	supramolecular complex	2	1
GO:0110165	cellular anatomical entity	1	3
GO:0005739	mitochondrion	5	2
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1

Expansion

Sequence features

E9AT30

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AT30

Function

Biological processes
Term	Name	Level	Count
GO:0000027	ribosomal large subunit assembly	7	1
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:1902775	mitochondrial large ribosomal subunit assembly	8	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0016070	RNA metabolic process	5	1
GO:0016071	mRNA metabolic process	6	1
GO:0016556	mRNA modification	6	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	26
GO:0003676	nucleic acid binding	3	26
GO:0003824	catalytic activity	1	26
GO:0004386	helicase activity	2	26
GO:0005488	binding	1	26
GO:0005524	ATP binding	5	26
GO:0017076	purine nucleotide binding	4	26
GO:0030554	adenyl nucleotide binding	5	26
GO:0032553	ribonucleotide binding	3	26
GO:0032555	purine ribonucleotide binding	4	26
GO:0032559	adenyl ribonucleotide binding	5	26
GO:0035639	purine ribonucleoside triphosphate binding	4	26
GO:0036094	small molecule binding	2	26
GO:0043167	ion binding	2	26
GO:0043168	anion binding	3	26
GO:0097159	organic cyclic compound binding	2	26
GO:0097367	carbohydrate derivative binding	2	26
GO:0140640	catalytic activity, acting on a nucleic acid	2	26
GO:0140657	ATP-dependent activity	1	26
GO:1901265	nucleoside phosphate binding	3	26
GO:1901363	heterocyclic compound binding	2	26
GO:0003723	RNA binding	4	3
GO:0016787	hydrolase activity	2	11
GO:0003724	RNA helicase activity	3	6
GO:0003743	translation initiation factor activity	4	5
GO:0008135	translation factor activity, RNA binding	3	5
GO:0008186	ATP-dependent activity, acting on RNA	2	6
GO:0045182	translation regulator activity	1	5
GO:0090079	translation regulator activity, nucleic acid binding	2	5
GO:0140098	catalytic activity, acting on RNA	3	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	73	77	PF00656	0.729
CLV_NRD_NRD_1	143	145	PF00675	0.441
CLV_NRD_NRD_1	217	219	PF00675	0.333
CLV_NRD_NRD_1	271	273	PF00675	0.281
CLV_NRD_NRD_1	326	328	PF00675	0.303
CLV_NRD_NRD_1	359	361	PF00675	0.369
CLV_NRD_NRD_1	61	63	PF00675	0.634
CLV_NRD_NRD_1	670	672	PF00675	0.596
CLV_NRD_NRD_1	687	689	PF00675	0.677
CLV_PCSK_FUR_1	668	672	PF00082	0.566
CLV_PCSK_KEX2_1	145	147	PF00082	0.419
CLV_PCSK_KEX2_1	217	219	PF00082	0.292
CLV_PCSK_KEX2_1	271	273	PF00082	0.281
CLV_PCSK_KEX2_1	359	361	PF00082	0.228
CLV_PCSK_KEX2_1	61	63	PF00082	0.634
CLV_PCSK_KEX2_1	670	672	PF00082	0.602
CLV_PCSK_KEX2_1	687	689	PF00082	0.678
CLV_PCSK_PC1ET2_1	145	147	PF00082	0.387
CLV_PCSK_PC7_1	267	273	PF00082	0.323
CLV_PCSK_SKI1_1	11	15	PF00082	0.529
CLV_PCSK_SKI1_1	148	152	PF00082	0.526
CLV_PCSK_SKI1_1	165	169	PF00082	0.209
CLV_PCSK_SKI1_1	382	386	PF00082	0.264
CLV_PCSK_SKI1_1	397	401	PF00082	0.238
CLV_PCSK_SKI1_1	415	419	PF00082	0.586
CLV_PCSK_SKI1_1	422	426	PF00082	0.407
CLV_PCSK_SKI1_1	62	66	PF00082	0.486
CLV_PCSK_SKI1_1	626	630	PF00082	0.355
DEG_APCC_DBOX_1	134	142	PF00400	0.365
DEG_APCC_DBOX_1	326	334	PF00400	0.480
DEG_APCC_DBOX_1	421	429	PF00400	0.408
DEG_APCC_DBOX_1	479	487	PF00400	0.552
DEG_APCC_DBOX_1	61	69	PF00400	0.435
DEG_Nend_Nbox_1	1	3	PF02207	0.495
DEG_SPOP_SBC_1	446	450	PF00917	0.492
DOC_CKS1_1	428	433	PF01111	0.464
DOC_CYCLIN_RxL_1	394	403	PF00134	0.435
DOC_CYCLIN_RxL_1	412	420	PF00134	0.572
DOC_CYCLIN_yCln2_LP_2	109	115	PF00134	0.532
DOC_CYCLIN_yCln2_LP_2	2	8	PF00134	0.496
DOC_CYCLIN_yCln2_LP_2	428	434	PF00134	0.590
DOC_CYCLIN_yCln2_LP_2	641	644	PF00134	0.409
DOC_MAPK_gen_1	267	276	PF00069	0.468
DOC_MAPK_JIP1_4	394	400	PF00069	0.441
DOC_PP1_RVXF_1	482	489	PF00149	0.422
DOC_PP1_RVXF_1	496	502	PF00149	0.287
DOC_PP1_RVXF_1	511	518	PF00149	0.511
DOC_PP1_RVXF_1	573	580	PF00149	0.430
DOC_PP1_SILK_1	131	136	PF00149	0.374
DOC_PP2B_LxvP_1	109	112	PF13499	0.549
DOC_PP2B_LxvP_1	641	644	PF13499	0.409
DOC_PP4_FxxP_1	252	255	PF00568	0.498
DOC_PP4_FxxP_1	619	622	PF00568	0.357
DOC_PP4_FxxP_1	663	666	PF00568	0.526
DOC_USP7_MATH_1	253	257	PF00917	0.544
DOC_USP7_MATH_1	265	269	PF00917	0.530
DOC_USP7_MATH_1	437	441	PF00917	0.620
DOC_USP7_MATH_1	446	450	PF00917	0.688
DOC_USP7_MATH_1	547	551	PF00917	0.444
DOC_USP7_MATH_1	650	654	PF00917	0.434
DOC_USP7_MATH_1	675	679	PF00917	0.670
DOC_WW_Pin1_4	127	132	PF00397	0.517
DOC_WW_Pin1_4	148	153	PF00397	0.439
DOC_WW_Pin1_4	340	345	PF00397	0.488
DOC_WW_Pin1_4	427	432	PF00397	0.483
DOC_WW_Pin1_4	442	447	PF00397	0.689
DOC_WW_Pin1_4	448	453	PF00397	0.716
DOC_WW_Pin1_4	676	681	PF00397	0.673
DOC_WW_Pin1_4	90	95	PF00397	0.592
LIG_14-3-3_CanoR_1	28	34	PF00244	0.551
LIG_14-3-3_CanoR_1	359	365	PF00244	0.492
LIG_14-3-3_CanoR_1	43	50	PF00244	0.481
LIG_14-3-3_CanoR_1	436	446	PF00244	0.760
LIG_14-3-3_CanoR_1	560	568	PF00244	0.517
LIG_Actin_WH2_2	179	194	PF00022	0.453
LIG_Actin_WH2_2	53	71	PF00022	0.478
LIG_AP2alpha_1	654	658	PF02296	0.529
LIG_APCC_ABBA_1	233	238	PF00400	0.523
LIG_BIR_III_4	107	111	PF00653	0.546
LIG_BIR_III_4	37	41	PF00653	0.569
LIG_BRCT_BRCA1_1	301	305	PF00533	0.421
LIG_BRCT_BRCA1_1	419	423	PF00533	0.383
LIG_CtBP_PxDLS_1	94	98	PF00389	0.426
LIG_EH1_1	311	319	PF00400	0.426
LIG_eIF4E_1	181	187	PF01652	0.523
LIG_FHA_1	412	418	PF00498	0.504
LIG_FHA_1	503	509	PF00498	0.457
LIG_FHA_1	563	569	PF00498	0.483
LIG_FHA_1	677	683	PF00498	0.684
LIG_FHA_2	203	209	PF00498	0.485
LIG_FHA_2	428	434	PF00498	0.559
LIG_FHA_2	590	596	PF00498	0.445
LIG_IRF3_LxIS_1	235	242	PF10401	0.391
LIG_LIR_Apic_2	250	255	PF02991	0.498
LIG_LIR_Apic_2	618	622	PF02991	0.349
LIG_LIR_Apic_2	661	666	PF02991	0.521
LIG_LIR_Gen_1	122	131	PF02991	0.561
LIG_LIR_Gen_1	653	662	PF02991	0.592
LIG_LIR_Nem_3	122	126	PF02991	0.509
LIG_LIR_Nem_3	163	167	PF02991	0.520
LIG_LIR_Nem_3	22	27	PF02991	0.720
LIG_LIR_Nem_3	302	308	PF02991	0.556
LIG_LIR_Nem_3	578	582	PF02991	0.430
LIG_LIR_Nem_3	653	657	PF02991	0.572
LIG_LIR_Nem_3	686	692	PF02991	0.554
LIG_LIR_Nem_3	98	104	PF02991	0.448
LIG_NRBOX	185	191	PF00104	0.480
LIG_OCRL_FandH_1	304	316	PF00620	0.426
LIG_PCNA_PIPBox_1	694	703	PF02747	0.542
LIG_Pex14_2	654	658	PF04695	0.529
LIG_PTB_Apo_2	657	664	PF02174	0.556
LIG_SH2_CRK	101	105	PF00017	0.538
LIG_SH2_CRK	147	151	PF00017	0.567
LIG_SH2_CRK	183	187	PF00017	0.509
LIG_SH2_CRK	588	592	PF00017	0.497
LIG_SH2_NCK_1	588	592	PF00017	0.497
LIG_SH2_STAP1	183	187	PF00017	0.523
LIG_SH2_STAT3	634	637	PF00017	0.450
LIG_SH2_STAT3	649	652	PF00017	0.350
LIG_SH2_STAT5	181	184	PF00017	0.466
LIG_SH2_STAT5	236	239	PF00017	0.441
LIG_SH2_STAT5	329	332	PF00017	0.543
LIG_SH2_STAT5	526	529	PF00017	0.481
LIG_SH2_STAT5	584	587	PF00017	0.469
LIG_SH2_STAT5	615	618	PF00017	0.314
LIG_SH2_STAT5	634	637	PF00017	0.551
LIG_SH3_2	666	671	PF14604	0.582
LIG_SH3_3	2	8	PF00018	0.517
LIG_SH3_3	280	286	PF00018	0.495
LIG_SH3_3	287	293	PF00018	0.514
LIG_SH3_3	338	344	PF00018	0.556
LIG_SH3_3	389	395	PF00018	0.571
LIG_SH3_3	449	455	PF00018	0.771
LIG_SH3_3	61	67	PF00018	0.687
LIG_SH3_3	663	669	PF00018	0.540
LIG_SH3_3	86	92	PF00018	0.568
LIG_SUMO_SIM_par_1	102	107	PF11976	0.381
LIG_SUMO_SIM_par_1	158	163	PF11976	0.442
LIG_TRAF2_1	322	325	PF00917	0.441
LIG_TRAF2_1	80	83	PF00917	0.564
LIG_TYR_ITIM	162	167	PF00017	0.523
LIG_TYR_ITIM	407	412	PF00017	0.415
LIG_UBA3_1	186	192	PF00899	0.533
LIG_UBA3_1	347	355	PF00899	0.441
LIG_UBA3_1	384	390	PF00899	0.422
LIG_UBA3_1	424	429	PF00899	0.342
LIG_WRC_WIRS_1	220	225	PF05994	0.426
LIG_WRC_WIRS_1	616	621	PF05994	0.405
MOD_CDC14_SPxK_1	451	454	PF00782	0.549
MOD_CDK_SPxK_1	448	454	PF00069	0.541
MOD_CK1_1	299	305	PF00069	0.446
MOD_CK1_1	32	38	PF00069	0.736
MOD_CK1_1	343	349	PF00069	0.428
MOD_CK1_1	445	451	PF00069	0.593
MOD_CK1_1	45	51	PF00069	0.708
MOD_CK1_1	545	551	PF00069	0.449
MOD_CK2_1	237	243	PF00069	0.496
MOD_CK2_1	253	259	PF00069	0.448
MOD_CK2_1	279	285	PF00069	0.442
MOD_CK2_1	318	324	PF00069	0.443
MOD_CK2_1	427	433	PF00069	0.600
MOD_CK2_1	547	553	PF00069	0.426
MOD_CK2_1	589	595	PF00069	0.430
MOD_CK2_1	77	83	PF00069	0.681
MOD_DYRK1A_RPxSP_1	148	152	PF00069	0.360
MOD_GlcNHglycan	21	24	PF01048	0.740
MOD_GlcNHglycan	255	258	PF01048	0.250
MOD_GlcNHglycan	301	304	PF01048	0.410
MOD_GlcNHglycan	31	34	PF01048	0.640
MOD_GlcNHglycan	321	324	PF01048	0.262
MOD_GlcNHglycan	439	442	PF01048	0.587
MOD_GlcNHglycan	549	552	PF01048	0.267
MOD_GlcNHglycan	96	100	PF01048	0.535
MOD_GSK3_1	129	136	PF00069	0.495
MOD_GSK3_1	255	262	PF00069	0.492
MOD_GSK3_1	28	35	PF00069	0.660
MOD_GSK3_1	442	449	PF00069	0.638
MOD_GSK3_1	466	473	PF00069	0.631
MOD_GSK3_1	538	545	PF00069	0.449
MOD_GSK3_1	558	565	PF00069	0.494
MOD_GSK3_1	567	574	PF00069	0.502
MOD_GSK3_1	589	596	PF00069	0.464
MOD_N-GLC_1	538	543	PF02516	0.323
MOD_N-GLC_1	658	663	PF02516	0.531
MOD_N-GLC_1	676	681	PF02516	0.541
MOD_NEK2_1	279	284	PF00069	0.488
MOD_NEK2_1	400	405	PF00069	0.470
MOD_NEK2_1	411	416	PF00069	0.436
MOD_NEK2_1	468	473	PF00069	0.521
MOD_NEK2_1	502	507	PF00069	0.467
MOD_NEK2_1	517	522	PF00069	0.454
MOD_NEK2_1	558	563	PF00069	0.532
MOD_NEK2_1	658	663	PF00069	0.511
MOD_NEK2_2	615	620	PF00069	0.303
MOD_PIKK_1	133	139	PF00454	0.625
MOD_PIKK_1	296	302	PF00454	0.429
MOD_PIKK_1	50	56	PF00454	0.456
MOD_PIKK_1	533	539	PF00454	0.532
MOD_PIKK_1	70	76	PF00454	0.518
MOD_PKA_2	318	324	PF00069	0.464
MOD_PKA_2	42	48	PF00069	0.498
MOD_PKA_2	683	689	PF00069	0.650
MOD_Plk_1	237	243	PF00069	0.501
MOD_Plk_1	45	51	PF00069	0.456
MOD_Plk_1	538	544	PF00069	0.484
MOD_Plk_1	658	664	PF00069	0.548
MOD_Plk_2-3	589	595	PF00069	0.452
MOD_Plk_4	129	135	PF00069	0.477
MOD_Plk_4	182	188	PF00069	0.446
MOD_Plk_4	219	225	PF00069	0.426
MOD_Plk_4	343	349	PF00069	0.439
MOD_Plk_4	360	366	PF00069	0.505
MOD_Plk_4	563	569	PF00069	0.538
MOD_Plk_4	615	621	PF00069	0.370
MOD_Plk_4	650	656	PF00069	0.477
MOD_Plk_4	658	664	PF00069	0.503
MOD_ProDKin_1	127	133	PF00069	0.520
MOD_ProDKin_1	148	154	PF00069	0.320
MOD_ProDKin_1	340	346	PF00069	0.488
MOD_ProDKin_1	427	433	PF00069	0.484
MOD_ProDKin_1	442	448	PF00069	0.692
MOD_ProDKin_1	676	682	PF00069	0.677
MOD_ProDKin_1	90	96	PF00069	0.587
TRG_DiLeu_BaEn_1	285	290	PF01217	0.430
TRG_DiLeu_BaLyEn_6	394	399	PF01217	0.433
TRG_ENDOCYTIC_2	101	104	PF00928	0.494
TRG_ENDOCYTIC_2	147	150	PF00928	0.525
TRG_ENDOCYTIC_2	164	167	PF00928	0.583
TRG_ENDOCYTIC_2	183	186	PF00928	0.547
TRG_ENDOCYTIC_2	236	239	PF00928	0.441
TRG_ENDOCYTIC_2	409	412	PF00928	0.385
TRG_ENDOCYTIC_2	617	620	PF00928	0.355
TRG_ER_diArg_1	144	147	PF00400	0.484
TRG_ER_diArg_1	358	360	PF00400	0.457
TRG_ER_diArg_1	60	62	PF00400	0.421
TRG_ER_diArg_1	668	671	PF00400	0.730
TRG_ER_diArg_1	687	689	PF00400	0.646
TRG_NLS_MonoExtN_4	144	149	PF00514	0.394
TRG_Pf-PMV_PEXEL_1	306	311	PF00026	0.337
TRG_Pf-PMV_PEXEL_1	422	427	PF00026	0.585

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I458	Leptomonas seymouri	76%	100%
A0A0N1I5R1	Leptomonas seymouri	22%	89%
A0A0S4IX79	Bodo saltans	24%	97%
A0A0S4JPF4	Bodo saltans	39%	100%
A0A1X0P7J3	Trypanosomatidae	57%	100%
A0A3Q8ID91	Leishmania donovani	26%	100%
A0A3Q8IF94	Leishmania donovani	25%	100%
A0A3Q8IG57	Leishmania donovani	92%	90%
A0A3S7WR01	Leishmania donovani	23%	90%
A4HGR1	Leishmania braziliensis	27%	100%
A4HK20	Leishmania braziliensis	25%	100%
A4HPB9	Leishmania braziliensis	88%	100%
A4HUC1	Leishmania infantum	23%	90%
A4I3T6	Leishmania infantum	26%	100%
A4I7K4	Leishmania infantum	25%	100%
A4ICF9	Leishmania infantum	93%	100%
D0A3C9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	100%
E9AN20	Leishmania mexicana (strain MHOM/GT/2001/U1103)	21%	100%
E9B028	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9B2G1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q1G8	Leishmania major	93%	100%
Q4Q5P5	Leishmania major	25%	100%
Q4Q858	Leishmania major	26%	100%
V5D8J9	Trypanosoma cruzi	57%	100%