RING-type E3 ubiquitin transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Ring finger domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9ASW7_LEIMU

TriTrypDb:

LmxM.36.1930

Length:

299

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	9
GO:0016020	membrane	2	8
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	9
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	9
GO:0110165	cellular anatomical entity	1	9

Expansion

Sequence features

E9ASW7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASW7

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	9
GO:0006511	ubiquitin-dependent protein catabolic process	7	9
GO:0006807	nitrogen compound metabolic process	2	9
GO:0008152	metabolic process	1	9
GO:0009056	catabolic process	2	9
GO:0009057	macromolecule catabolic process	4	9
GO:0009987	cellular process	1	9
GO:0019538	protein metabolic process	3	9
GO:0019941	modification-dependent protein catabolic process	6	9
GO:0043170	macromolecule metabolic process	3	9
GO:0043632	modification-dependent macromolecule catabolic process	5	9
GO:0044237	cellular metabolic process	2	9
GO:0044238	primary metabolic process	2	9
GO:0044248	cellular catabolic process	3	9
GO:0044260	obsolete cellular macromolecule metabolic process	3	9
GO:0044265	obsolete cellular macromolecule catabolic process	4	9
GO:0051603	proteolysis involved in protein catabolic process	5	9
GO:0071704	organic substance metabolic process	2	9
GO:1901564	organonitrogen compound metabolic process	3	9
GO:1901575	organic substance catabolic process	3	9
GO:0006515	protein quality control for misfolded or incompletely synthesized proteins	6	1
GO:0006950	response to stress	2	1
GO:0010033	response to organic substance	3	1
GO:0010243	response to organonitrogen compound	4	1
GO:0010498	proteasomal protein catabolic process	5	1
GO:0030163	protein catabolic process	4	1
GO:0033554	cellular response to stress	3	1
GO:0034976	response to endoplasmic reticulum stress	4	1
GO:0036503	ERAD pathway	5	1
GO:0042221	response to chemical	2	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0071712	ER-associated misfolded protein catabolic process	6	1
GO:1901565	organonitrogen compound catabolic process	4	1
GO:1901698	response to nitrogen compound	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004842	ubiquitin-protein transferase activity	4	9
GO:0005488	binding	1	9
GO:0016740	transferase activity	2	9
GO:0019787	ubiquitin-like protein transferase activity	3	9
GO:0043167	ion binding	2	9
GO:0043169	cation binding	3	9
GO:0046872	metal ion binding	4	9
GO:0061630	ubiquitin protein ligase activity	5	9
GO:0061659	ubiquitin-like protein ligase activity	4	9
GO:0140096	catalytic activity, acting on a protein	2	9
GO:0005515	protein binding	2	1
GO:0019899	enzyme binding	3	1
GO:0044390	ubiquitin-like protein conjugating enzyme binding	4	1
GO:0016746	acyltransferase activity	3	1
GO:0061631	ubiquitin conjugating enzyme activity	5	1
GO:0061650	ubiquitin-like protein conjugating enzyme activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	130	132	PF00675	0.524
CLV_PCSK_KEX2_1	130	132	PF00082	0.522
DOC_CYCLIN_RxL_1	277	285	PF00134	0.208
DOC_MAPK_MEF2A_6	174	181	PF00069	0.771
DOC_MAPK_MEF2A_6	280	288	PF00069	0.391
DOC_MAPK_NFAT4_5	174	182	PF00069	0.770
DOC_PP4_FxxP_1	115	118	PF00568	0.737
DOC_USP7_MATH_1	104	108	PF00917	0.804
DOC_USP7_MATH_1	134	138	PF00917	0.787
DOC_USP7_MATH_1	233	237	PF00917	0.575
DOC_USP7_MATH_1	262	266	PF00917	0.610
DOC_USP7_MATH_1	99	103	PF00917	0.809
DOC_WW_Pin1_4	249	254	PF00397	0.569
DOC_WW_Pin1_4	271	276	PF00397	0.552
LIG_BRCT_BRCA1_1	182	186	PF00533	0.506
LIG_deltaCOP1_diTrp_1	214	222	PF00928	0.445
LIG_FHA_1	19	25	PF00498	0.560
LIG_FHA_1	190	196	PF00498	0.412
LIG_FHA_2	103	109	PF00498	0.715
LIG_FHA_2	17	23	PF00498	0.548
LIG_FHA_2	233	239	PF00498	0.437
LIG_FHA_2	96	102	PF00498	0.744
LIG_GBD_Chelix_1	278	286	PF00786	0.492
LIG_GBD_Chelix_1	288	296	PF00786	0.425
LIG_LIR_Apic_2	112	118	PF02991	0.724
LIG_LIR_Gen_1	214	223	PF02991	0.407
LIG_LIR_Gen_1	285	295	PF02991	0.457
LIG_LIR_LC3C_4	197	201	PF02991	0.298
LIG_LIR_Nem_3	214	218	PF02991	0.410
LIG_LIR_Nem_3	285	290	PF02991	0.457
LIG_NRBOX	291	297	PF00104	0.422
LIG_Rb_LxCxE_1	26	49	PF01857	0.598
LIG_REV1ctd_RIR_1	112	117	PF16727	0.735
LIG_SH2_CRK	223	227	PF00017	0.409
LIG_SH3_3	103	109	PF00018	0.791
LIG_SH3_3	153	159	PF00018	0.735
LIG_SH3_3	270	276	PF00018	0.444
LIG_SH3_3	45	51	PF00018	0.525
LIG_SUMO_SIM_anti_2	197	202	PF11976	0.363
LIG_SUMO_SIM_par_1	199	205	PF11976	0.492
LIG_TRAF2_1	109	112	PF00917	0.726
LIG_TRAF2_1	136	139	PF00917	0.740
LIG_TRAF2_1	235	238	PF00917	0.415
LIG_UBA3_1	65	71	PF00899	0.634
LIG_WW_3	155	159	PF00397	0.739
MOD_CK1_1	102	108	PF00069	0.789
MOD_CK1_1	116	122	PF00069	0.672
MOD_CK1_1	261	267	PF00069	0.600
MOD_CK1_1	274	280	PF00069	0.225
MOD_CK1_1	81	87	PF00069	0.649
MOD_CK1_1	89	95	PF00069	0.723
MOD_CK2_1	133	139	PF00069	0.620
MOD_CK2_1	16	22	PF00069	0.548
MOD_CK2_1	232	238	PF00069	0.528
MOD_CK2_1	261	267	PF00069	0.558
MOD_CK2_1	95	101	PF00069	0.636
MOD_CMANNOS	212	215	PF00535	0.657
MOD_DYRK1A_RPxSP_1	249	253	PF00069	0.467
MOD_GlcNHglycan	101	104	PF01048	0.592
MOD_GlcNHglycan	118	121	PF01048	0.427
MOD_GlcNHglycan	182	185	PF01048	0.555
MOD_GlcNHglycan	260	263	PF01048	0.767
MOD_GlcNHglycan	4	7	PF01048	0.399
MOD_GlcNHglycan	42	45	PF01048	0.364
MOD_GlcNHglycan	79	83	PF01048	0.575
MOD_GlcNHglycan	86	89	PF01048	0.579
MOD_GSK3_1	241	248	PF00069	0.551
MOD_GSK3_1	258	265	PF00069	0.560
MOD_GSK3_1	74	81	PF00069	0.715
MOD_GSK3_1	82	89	PF00069	0.707
MOD_GSK3_1	90	97	PF00069	0.681
MOD_N-GLC_1	233	238	PF02516	0.747
MOD_NEK2_1	1	6	PF00069	0.720
MOD_NEK2_1	282	287	PF00069	0.438
MOD_NEK2_2	202	207	PF00069	0.492
MOD_PIKK_1	134	140	PF00454	0.819
MOD_PIKK_1	233	239	PF00454	0.549
MOD_PKA_2	116	122	PF00069	0.736
MOD_PKA_2	126	132	PF00069	0.763
MOD_PKA_2	245	251	PF00069	0.587
MOD_Plk_4	274	280	PF00069	0.304
MOD_Plk_4	282	288	PF00069	0.333
MOD_ProDKin_1	249	255	PF00069	0.570
MOD_ProDKin_1	271	277	PF00069	0.546
TRG_ENDOCYTIC_2	223	226	PF00928	0.406
TRG_ER_diArg_1	157	160	PF00400	0.729

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6L3	Leptomonas seymouri	56%	99%
A0A3Q8IKK1	Leishmania donovani	90%	100%
A0A3R7KB29	Trypanosoma rangeli	39%	100%
A4HP59	Leishmania braziliensis	76%	100%
A4IDG5	Leishmania infantum	88%	98%
Q4Q1N0	Leishmania major	90%	100%
V5BPK7	Trypanosoma cruzi	36%	100%