Putative tRNA pseudouridine synthase A

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase A, putative

Species:

Leishmania mexicana

UniProt:

E9AST8_LEIMU

TriTrypDb:

LmxM.36.1660

Length:

694

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9AST8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AST8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006364	rRNA processing	8	4
GO:0006396	RNA processing	6	4
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008152	metabolic process	1	8
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0016072	rRNA metabolic process	7	4
GO:0034470	ncRNA processing	7	4
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0034660	ncRNA metabolic process	6	4
GO:0043170	macromolecule metabolic process	3	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0001522	pseudouridine synthesis	6	4
GO:0009451	RNA modification	5	4
GO:0043412	macromolecule modification	4	4

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004730	pseudouridylate synthase activity	5	8
GO:0005488	binding	1	12
GO:0009982	pseudouridine synthase activity	4	12
GO:0016829	lyase activity	2	8
GO:0016835	carbon-oxygen lyase activity	3	8
GO:0016836	hydro-lyase activity	4	8
GO:0016853	isomerase activity	2	12
GO:0016866	intramolecular transferase activity	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	131	135	PF00656	0.700
CLV_C14_Caspase3-7	406	410	PF00656	0.456
CLV_C14_Caspase3-7	604	608	PF00656	0.500
CLV_C14_Caspase3-7	613	617	PF00656	0.466
CLV_NRD_NRD_1	108	110	PF00675	0.727
CLV_NRD_NRD_1	214	216	PF00675	0.810
CLV_NRD_NRD_1	255	257	PF00675	0.763
CLV_NRD_NRD_1	297	299	PF00675	0.693
CLV_NRD_NRD_1	308	310	PF00675	0.713
CLV_NRD_NRD_1	346	348	PF00675	0.276
CLV_NRD_NRD_1	48	50	PF00675	0.683
CLV_NRD_NRD_1	529	531	PF00675	0.296
CLV_NRD_NRD_1	56	58	PF00675	0.671
CLV_NRD_NRD_1	582	584	PF00675	0.483
CLV_NRD_NRD_1	590	592	PF00675	0.470
CLV_NRD_NRD_1	637	639	PF00675	0.586
CLV_NRD_NRD_1	659	661	PF00675	0.684
CLV_PCSK_FUR_1	54	58	PF00082	0.721
CLV_PCSK_FUR_1	588	592	PF00082	0.476
CLV_PCSK_KEX2_1	108	110	PF00082	0.727
CLV_PCSK_KEX2_1	255	257	PF00082	0.763
CLV_PCSK_KEX2_1	297	299	PF00082	0.698
CLV_PCSK_KEX2_1	308	310	PF00082	0.713
CLV_PCSK_KEX2_1	346	348	PF00082	0.276
CLV_PCSK_KEX2_1	50	52	PF00082	0.731
CLV_PCSK_KEX2_1	56	58	PF00082	0.627
CLV_PCSK_KEX2_1	582	584	PF00082	0.483
CLV_PCSK_KEX2_1	590	592	PF00082	0.470
CLV_PCSK_KEX2_1	631	633	PF00082	0.545
CLV_PCSK_KEX2_1	637	639	PF00082	0.582
CLV_PCSK_KEX2_1	659	661	PF00082	0.684
CLV_PCSK_PC1ET2_1	50	52	PF00082	0.731
CLV_PCSK_PC1ET2_1	631	633	PF00082	0.336
CLV_PCSK_PC7_1	251	257	PF00082	0.643
CLV_PCSK_SKI1_1	215	219	PF00082	0.639
CLV_PCSK_SKI1_1	256	260	PF00082	0.630
CLV_PCSK_SKI1_1	261	265	PF00082	0.628
CLV_PCSK_SKI1_1	470	474	PF00082	0.381
CLV_PCSK_SKI1_1	554	558	PF00082	0.425
CLV_PCSK_SKI1_1	596	600	PF00082	0.487
CLV_PCSK_SKI1_1	615	619	PF00082	0.364
DEG_APCC_DBOX_1	152	160	PF00400	0.635
DEG_APCC_DBOX_1	462	470	PF00400	0.524
DOC_CKS1_1	45	50	PF01111	0.755
DOC_MAPK_DCC_7	386	396	PF00069	0.448
DOC_MAPK_gen_1	108	115	PF00069	0.748
DOC_MAPK_gen_1	505	514	PF00069	0.471
DOC_MAPK_gen_1	530	540	PF00069	0.260
DOC_MAPK_gen_1	659	667	PF00069	0.563
DOC_MAPK_MEF2A_6	388	396	PF00069	0.445
DOC_MAPK_MEF2A_6	505	514	PF00069	0.435
DOC_MIT_MIM_1	150	160	PF04212	0.516
DOC_PP1_RVXF_1	384	390	PF00149	0.480
DOC_PP1_RVXF_1	535	541	PF00149	0.401
DOC_PP4_FxxP_1	389	392	PF00568	0.456
DOC_PP4_FxxP_1	499	502	PF00568	0.479
DOC_PP4_FxxP_1	647	650	PF00568	0.432
DOC_USP7_MATH_1	116	120	PF00917	0.632
DOC_USP7_MATH_1	25	29	PF00917	0.564
DOC_USP7_MATH_1	319	323	PF00917	0.684
DOC_USP7_MATH_1	430	434	PF00917	0.476
DOC_USP7_MATH_1	503	507	PF00917	0.505
DOC_USP7_MATH_1	549	553	PF00917	0.437
DOC_USP7_MATH_1	654	658	PF00917	0.748
DOC_USP7_MATH_1	84	88	PF00917	0.762
DOC_WW_Pin1_4	141	146	PF00397	0.578
DOC_WW_Pin1_4	203	208	PF00397	0.746
DOC_WW_Pin1_4	266	271	PF00397	0.668
DOC_WW_Pin1_4	288	293	PF00397	0.699
DOC_WW_Pin1_4	44	49	PF00397	0.756
DOC_WW_Pin1_4	596	601	PF00397	0.498
DOC_WW_Pin1_4	93	98	PF00397	0.644
LIG_14-3-3_CanoR_1	215	224	PF00244	0.598
LIG_14-3-3_CanoR_1	308	313	PF00244	0.680
LIG_14-3-3_CanoR_1	507	511	PF00244	0.434
LIG_14-3-3_CanoR_1	537	541	PF00244	0.387
LIG_14-3-3_CanoR_1	637	646	PF00244	0.529
LIG_14-3-3_CanoR_1	659	663	PF00244	0.684
LIG_Actin_WH2_2	144	162	PF00022	0.596
LIG_Actin_WH2_2	621	639	PF00022	0.609
LIG_BIR_III_4	409	413	PF00653	0.456
LIG_BRCT_BRCA1_1	168	172	PF00533	0.749
LIG_deltaCOP1_diTrp_1	575	581	PF00928	0.522
LIG_EH_1	366	370	PF12763	0.476
LIG_FHA_1	147	153	PF00498	0.530
LIG_FHA_1	240	246	PF00498	0.528
LIG_FHA_1	35	41	PF00498	0.673
LIG_FHA_1	462	468	PF00498	0.486
LIG_FHA_1	481	487	PF00498	0.306
LIG_FHA_2	226	232	PF00498	0.688
LIG_FHA_2	34	40	PF00498	0.660
LIG_FHA_2	597	603	PF00498	0.487
LIG_FHA_2	608	614	PF00498	0.467
LIG_Integrin_isoDGR_2	489	491	PF01839	0.581
LIG_Integrin_RGD_1	132	134	PF01839	0.756
LIG_LIR_Apic_2	497	502	PF02991	0.460
LIG_LIR_Apic_2	644	650	PF02991	0.430
LIG_LIR_Gen_1	610	618	PF02991	0.479
LIG_LIR_Nem_3	539	543	PF02991	0.414
LIG_LIR_Nem_3	575	581	PF02991	0.528
LIG_LIR_Nem_3	610	614	PF02991	0.531
LIG_PCNA_PIPBox_1	1	10	PF02747	0.550
LIG_PTB_Apo_2	492	499	PF02174	0.467
LIG_PTB_Apo_2	605	612	PF02174	0.526
LIG_PTB_Phospho_1	605	611	PF10480	0.524
LIG_RPA_C_Fungi	342	354	PF08784	0.449
LIG_SH2_CRK	397	401	PF00017	0.328
LIG_SH2_SRC	611	614	PF00017	0.543
LIG_SH2_STAP1	508	512	PF00017	0.503
LIG_SH2_STAP1	562	566	PF00017	0.584
LIG_SH2_STAT5	465	468	PF00017	0.321
LIG_SH2_STAT5	580	583	PF00017	0.603
LIG_SH2_STAT5	609	612	PF00017	0.606
LIG_SH2_STAT5	7	10	PF00017	0.673
LIG_SH3_2	292	297	PF14604	0.553
LIG_SH3_3	204	210	PF00018	0.488
LIG_SH3_3	289	295	PF00018	0.680
LIG_SH3_3	553	559	PF00018	0.423
LIG_SH3_3	566	572	PF00018	0.462
LIG_SUMO_SIM_par_1	440	445	PF11976	0.328
LIG_TRAF2_1	234	237	PF00917	0.743
LIG_TRAF2_1	24	27	PF00917	0.602
LIG_TRAF2_1	610	613	PF00917	0.503
LIG_TRAF2_1	677	680	PF00917	0.675
LIG_TRAF2_2	145	150	PF00917	0.558
LIG_UBA3_1	156	163	PF00899	0.510
LIG_UBA3_1	524	531	PF00899	0.286
LIG_WRC_WIRS_1	61	66	PF05994	0.729
LIG_WW_1	559	562	PF00397	0.461
LIG_WW_3	390	394	PF00397	0.447
MOD_CDC14_SPxK_1	269	272	PF00782	0.506
MOD_CDK_SPK_2	44	49	PF00069	0.562
MOD_CDK_SPK_2	596	601	PF00069	0.498
MOD_CDK_SPxK_1	266	272	PF00069	0.510
MOD_CDK_SPxK_1	44	50	PF00069	0.756
MOD_CDK_SPxxK_3	44	51	PF00069	0.754
MOD_CK1_1	506	512	PF00069	0.433
MOD_CK2_1	33	39	PF00069	0.752
MOD_CK2_1	342	348	PF00069	0.328
MOD_CK2_1	549	555	PF00069	0.444
MOD_CK2_1	596	602	PF00069	0.485
MOD_CK2_1	607	613	PF00069	0.473
MOD_Cter_Amidation	306	309	PF01082	0.656
MOD_Cter_Amidation	528	531	PF01082	0.288
MOD_GlcNHglycan	221	224	PF01048	0.672
MOD_GlcNHglycan	321	324	PF01048	0.644
MOD_GlcNHglycan	354	357	PF01048	0.339
MOD_GlcNHglycan	58	61	PF01048	0.673
MOD_GlcNHglycan	81	84	PF01048	0.664
MOD_GlcNHglycan	86	89	PF01048	0.651
MOD_GSK3_1	215	222	PF00069	0.568
MOD_GSK3_1	56	63	PF00069	0.687
MOD_GSK3_1	654	661	PF00069	0.719
MOD_N-GLC_1	361	366	PF02516	0.398
MOD_N-GLC_1	607	612	PF02516	0.530
MOD_NEK2_1	100	105	PF00069	0.629
MOD_NEK2_1	334	339	PF00069	0.479
MOD_NEK2_1	543	548	PF00069	0.439
MOD_NEK2_2	549	554	PF00069	0.442
MOD_NEK2_2	60	65	PF00069	0.643
MOD_NEK2_2	654	659	PF00069	0.750
MOD_PIKK_1	215	221	PF00454	0.501
MOD_PIKK_1	560	566	PF00454	0.595
MOD_PK_1	308	314	PF00069	0.684
MOD_PKA_1	215	221	PF00069	0.660
MOD_PKA_1	308	314	PF00069	0.742
MOD_PKA_1	56	62	PF00069	0.728
MOD_PKA_1	637	643	PF00069	0.510
MOD_PKA_2	280	286	PF00069	0.496
MOD_PKA_2	308	314	PF00069	0.655
MOD_PKA_2	506	512	PF00069	0.432
MOD_PKA_2	536	542	PF00069	0.395
MOD_PKA_2	56	62	PF00069	0.735
MOD_PKA_2	584	590	PF00069	0.466
MOD_PKA_2	637	643	PF00069	0.407
MOD_PKA_2	658	664	PF00069	0.737
MOD_PKB_1	54	62	PF00069	0.725
MOD_Plk_1	124	130	PF00069	0.708
MOD_Plk_1	283	289	PF00069	0.770
MOD_Plk_1	317	323	PF00069	0.530
MOD_Plk_1	448	454	PF00069	0.344
MOD_Plk_1	554	560	PF00069	0.434
MOD_Plk_1	607	613	PF00069	0.524
MOD_Plk_1	688	694	PF00069	0.630
MOD_Plk_2-3	607	613	PF00069	0.490
MOD_Plk_4	168	174	PF00069	0.606
MOD_Plk_4	308	314	PF00069	0.739
MOD_Plk_4	456	462	PF00069	0.348
MOD_Plk_4	60	66	PF00069	0.599
MOD_ProDKin_1	141	147	PF00069	0.572
MOD_ProDKin_1	203	209	PF00069	0.746
MOD_ProDKin_1	266	272	PF00069	0.669
MOD_ProDKin_1	288	294	PF00069	0.690
MOD_ProDKin_1	44	50	PF00069	0.756
MOD_ProDKin_1	596	602	PF00069	0.492
MOD_ProDKin_1	93	99	PF00069	0.644
MOD_SUMO_for_1	234	237	PF00179	0.719
MOD_SUMO_rev_2	154	161	PF00179	0.474
MOD_SUMO_rev_2	607	617	PF00179	0.482
TRG_DiLeu_BaEn_1	241	246	PF01217	0.727
TRG_DiLeu_BaEn_1	624	629	PF01217	0.346
TRG_DiLeu_BaEn_2	326	332	PF01217	0.555
TRG_DiLeu_BaEn_2	448	454	PF01217	0.385
TRG_ENDOCYTIC_2	138	141	PF00928	0.770
TRG_ENDOCYTIC_2	611	614	PF00928	0.606
TRG_ER_diArg_1	177	180	PF00400	0.553
TRG_ER_diArg_1	296	298	PF00400	0.703
TRG_ER_diArg_1	384	387	PF00400	0.496
TRG_ER_diArg_1	467	470	PF00400	0.344
TRG_ER_diArg_1	48	51	PF00400	0.561
TRG_ER_diArg_1	581	583	PF00400	0.494
TRG_ER_diArg_1	588	591	PF00400	0.478
TRG_ER_diArg_1	636	638	PF00400	0.624
TRG_Pf-PMV_PEXEL_1	261	265	PF00026	0.731
TRG_Pf-PMV_PEXEL_1	421	426	PF00026	0.344
TRG_Pf-PMV_PEXEL_1	590	594	PF00026	0.529
TRG_Pf-PMV_PEXEL_1	601	605	PF00026	0.448

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5W9	Leptomonas seymouri	69%	85%
A0A0S4IST3	Bodo saltans	40%	100%
A0A1X0P7U6	Trypanosomatidae	55%	100%
A0A3R7R867	Trypanosoma rangeli	54%	100%
A0A3S7XAP2	Leishmania donovani	92%	79%
A4HP30	Leishmania braziliensis	83%	100%
A4IDD7	Leishmania infantum	92%	79%
D0A313	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	53%	100%
Q4Q1Q8	Leishmania major	92%	100%
V5BPI0	Trypanosoma cruzi	55%	100%