Nfu_N domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NifU-like domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9ASK8_LEIMU

TriTrypDb:

LmxM.36.0880

Length:

301

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 17
NetGPI	no	yes: 0, no: 17

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ASK8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASK8

Function

Biological processes
Term	Name	Level	Count
GO:0006790	sulfur compound metabolic process	3	18
GO:0008152	metabolic process	1	18
GO:0009987	cellular process	1	18
GO:0016043	cellular component organization	3	18
GO:0016226	iron-sulfur cluster assembly	4	18
GO:0022607	cellular component assembly	4	18
GO:0031163	metallo-sulfur cluster assembly	5	18
GO:0044237	cellular metabolic process	2	18
GO:0071840	cellular component organization or biogenesis	2	18
GO:0006807	nitrogen compound metabolic process	2	2
GO:0019538	protein metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044238	primary metabolic process	2	2
GO:0051604	protein maturation	4	2
GO:0071704	organic substance metabolic process	2	2
GO:0097428	protein maturation by iron-sulfur cluster transfer	5	2
GO:1901564	organonitrogen compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	18
GO:0005506	iron ion binding	6	18
GO:0043167	ion binding	2	18
GO:0043169	cation binding	3	18
GO:0046872	metal ion binding	4	18
GO:0046914	transition metal ion binding	5	18
GO:0051536	iron-sulfur cluster binding	3	18
GO:0051540	metal cluster binding	2	18
GO:0051539	4 iron, 4 sulfur cluster binding	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	225	229	PF00656	0.440
CLV_NRD_NRD_1	44	46	PF00675	0.447
CLV_NRD_NRD_1	49	51	PF00675	0.467
CLV_PCSK_KEX2_1	44	46	PF00082	0.490
CLV_PCSK_KEX2_1	49	51	PF00082	0.480
CLV_PCSK_PC7_1	45	51	PF00082	0.578
CLV_PCSK_SKI1_1	17	21	PF00082	0.469
CLV_PCSK_SKI1_1	178	182	PF00082	0.270
CLV_PCSK_SKI1_1	232	236	PF00082	0.256
CLV_PCSK_SKI1_1	264	268	PF00082	0.598
CLV_Separin_Metazoa	179	183	PF03568	0.423
DOC_CKS1_1	57	62	PF01111	0.284
DOC_CYCLIN_RxL_1	175	183	PF00134	0.300
DOC_MAPK_MEF2A_6	204	212	PF00069	0.440
DOC_PP1_RVXF_1	129	135	PF00149	0.320
DOC_PP1_RVXF_1	15	21	PF00149	0.384
DOC_PP1_RVXF_1	195	202	PF00149	0.410
DOC_PP1_RVXF_1	87	94	PF00149	0.284
DOC_PP4_FxxP_1	113	116	PF00568	0.260
DOC_PP4_FxxP_1	67	70	PF00568	0.260
DOC_USP7_MATH_1	21	25	PF00917	0.461
DOC_USP7_MATH_1	70	74	PF00917	0.304
DOC_USP7_UBL2_3	264	268	PF12436	0.465
DOC_WW_Pin1_4	149	154	PF00397	0.356
DOC_WW_Pin1_4	283	288	PF00397	0.559
DOC_WW_Pin1_4	56	61	PF00397	0.271
LIG_14-3-3_CanoR_1	2	7	PF00244	0.527
LIG_14-3-3_CanoR_1	37	43	PF00244	0.464
LIG_14-3-3_CanoR_1	44	48	PF00244	0.469
LIG_deltaCOP1_diTrp_1	128	134	PF00928	0.246
LIG_eIF4E_1	13	19	PF01652	0.385
LIG_eIF4E_1	253	259	PF01652	0.370
LIG_FHA_1	203	209	PF00498	0.440
LIG_FHA_1	240	246	PF00498	0.457
LIG_FHA_1	284	290	PF00498	0.505
LIG_FHA_2	123	129	PF00498	0.419
LIG_FHA_2	156	162	PF00498	0.467
LIG_FHA_2	167	173	PF00498	0.454
LIG_FHA_2	223	229	PF00498	0.423
LIG_LIR_Apic_2	111	116	PF02991	0.239
LIG_LIR_Apic_2	66	70	PF02991	0.322
LIG_LIR_Gen_1	122	132	PF02991	0.276
LIG_LIR_Gen_1	156	164	PF02991	0.433
LIG_LIR_Gen_1	291	298	PF02991	0.501
LIG_LIR_Gen_1	72	79	PF02991	0.280
LIG_LIR_Nem_3	122	127	PF02991	0.273
LIG_LIR_Nem_3	128	132	PF02991	0.262
LIG_LIR_Nem_3	156	160	PF02991	0.406
LIG_LIR_Nem_3	254	259	PF02991	0.379
LIG_LIR_Nem_3	291	295	PF02991	0.497
LIG_LIR_Nem_3	72	77	PF02991	0.286
LIG_Pex14_1	34	38	PF04695	0.355
LIG_SH2_CRK	256	260	PF00017	0.453
LIG_SH2_CRK	292	296	PF00017	0.565
LIG_SH2_GRB2like	124	127	PF00017	0.249
LIG_SH2_NCK_1	124	128	PF00017	0.249
LIG_SH2_NCK_1	157	161	PF00017	0.339
LIG_SH2_STAP1	105	109	PF00017	0.144
LIG_SH2_STAT5	124	127	PF00017	0.362
LIG_SH2_STAT5	143	146	PF00017	0.237
LIG_SH2_STAT5	157	160	PF00017	0.485
LIG_TYR_ITIM	290	295	PF00017	0.590
LIG_WRC_WIRS_1	136	141	PF05994	0.231
LIG_WRC_WIRS_1	74	79	PF05994	0.376
MOD_CDC14_SPxK_1	152	155	PF00782	0.357
MOD_CDK_SPxK_1	149	155	PF00069	0.364
MOD_CK1_1	122	128	PF00069	0.324
MOD_CK1_1	135	141	PF00069	0.173
MOD_CK1_1	222	228	PF00069	0.425
MOD_CK1_1	23	29	PF00069	0.664
MOD_CK1_1	7	13	PF00069	0.635
MOD_CK1_1	73	79	PF00069	0.404
MOD_CK2_1	122	128	PF00069	0.346
MOD_CK2_1	155	161	PF00069	0.425
MOD_CK2_1	166	172	PF00069	0.381
MOD_GSK3_1	19	26	PF00069	0.460
MOD_GSK3_1	2	9	PF00069	0.433
MOD_GSK3_1	202	209	PF00069	0.430
MOD_GSK3_1	215	222	PF00069	0.445
MOD_N-GLC_1	146	151	PF02516	0.363
MOD_N-GLC_1	7	12	PF02516	0.384
MOD_N-GLC_2	148	150	PF02516	0.406
MOD_NEK2_1	146	151	PF00069	0.403
MOD_NEK2_1	20	25	PF00069	0.635
MOD_NEK2_1	38	43	PF00069	0.482
MOD_NEK2_1	6	11	PF00069	0.478
MOD_NEK2_1	63	68	PF00069	0.392
MOD_PIKK_1	103	109	PF00454	0.144
MOD_PIKK_1	219	225	PF00454	0.423
MOD_PIKK_1	24	30	PF00454	0.380
MOD_PIKK_1	95	101	PF00454	0.320
MOD_PK_1	117	123	PF00069	0.260
MOD_PKA_2	43	49	PF00069	0.437
MOD_Plk_1	132	138	PF00069	0.242
MOD_Plk_1	146	152	PF00069	0.386
MOD_Plk_1	155	161	PF00069	0.472
MOD_Plk_4	108	114	PF00069	0.296
MOD_Plk_4	135	141	PF00069	0.236
MOD_Plk_4	155	161	PF00069	0.264
MOD_Plk_4	285	291	PF00069	0.513
MOD_Plk_4	38	44	PF00069	0.542
MOD_Plk_4	73	79	PF00069	0.332
MOD_ProDKin_1	149	155	PF00069	0.364
MOD_ProDKin_1	283	289	PF00069	0.558
MOD_ProDKin_1	56	62	PF00069	0.271
MOD_SUMO_rev_2	161	169	PF00179	0.504
MOD_SUMO_rev_2	192	199	PF00179	0.431
TRG_DiLeu_BaEn_1	254	259	PF01217	0.376
TRG_ENDOCYTIC_2	124	127	PF00928	0.301
TRG_ENDOCYTIC_2	143	146	PF00928	0.271
TRG_ENDOCYTIC_2	157	160	PF00928	0.437
TRG_ENDOCYTIC_2	256	259	PF00928	0.415
TRG_ENDOCYTIC_2	292	295	PF00928	0.536
TRG_ER_diArg_1	43	45	PF00400	0.495
TRG_ER_diArg_1	49	51	PF00400	0.482

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2Q0	Leptomonas seymouri	35%	100%
A0A0N1I735	Leptomonas seymouri	83%	100%
A0A0S4IJH0	Bodo saltans	35%	100%
A0A0S4IVB5	Bodo saltans	59%	100%
A0A1X0NST9	Trypanosomatidae	33%	100%
A0A1X0P8F8	Trypanosomatidae	69%	100%
A0A3S5H7F5	Leishmania donovani	32%	100%
A0A3S7XAI2	Leishmania donovani	95%	100%
A4HEM5	Leishmania braziliensis	35%	100%
A4HNU8	Leishmania braziliensis	94%	100%
A4I1V6	Leishmania infantum	32%	100%
A4ICP6	Leishmania infantum	95%	100%
B3MRT7	Drosophila ananassae	29%	100%
B3NYF7	Drosophila erecta	30%	100%
B4H303	Drosophila persimilis	29%	100%
B4IMF6	Drosophila sechellia	29%	100%
B4JWR9	Drosophila grimshawi	29%	100%
B4M375	Drosophila virilis	29%	100%
B4NE93	Drosophila willistoni	30%	100%
B4PZ52	Drosophila yakuba	30%	100%
B4R3T1	Drosophila simulans	31%	100%
B5DKJ8	Drosophila pseudoobscura pseudoobscura	29%	100%
C9ZS65	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
D0A2R6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	69%	100%
E9AY01	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
P32860	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
Q4Q1Y8	Leishmania major	91%	100%
Q4Q9H2	Leishmania major	33%	100%
Q8SY96	Drosophila melanogaster	29%	100%
Q9C8J2	Arabidopsis thaliana	33%	100%
Q9LIG6	Arabidopsis thaliana	32%	100%
Q9QZ23	Mus musculus	32%	100%
Q9UUB8	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	33%	100%
V5DRN0	Trypanosoma cruzi	73%	100%