General transcription factor IIH subunit 4

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

TFIIH basal transcription factor subunit, putative

Species:

Leishmania mexicana

UniProt:

E9ASK0_LEIMU

TriTrypDb:

LmxM.36.0800

Length:

559

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000439	transcription factor TFIIH core complex	4	12
GO:0005667	transcription regulator complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0090575	RNA polymerase II transcription regulator complex	3	12
GO:0140513	nuclear protein-containing complex	2	12
GO:0005654	nucleoplasm	2	1
GO:0005675	transcription factor TFIIH holo complex	4	1
GO:0032806	carboxy-terminal domain protein kinase complex	3	1
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1902494	catalytic complex	2	1
GO:1902554	serine/threonine protein kinase complex	6	1
GO:1902911	protein kinase complex	5	1
GO:1990234	transferase complex	3	1

Expansion

Sequence features

E9ASK0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASK0

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006289	nucleotide-excision repair	6	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006355	regulation of DNA-templated transcription	6	1
GO:0006357	regulation of transcription by RNA polymerase II	7	1
GO:0006468	protein phosphorylation	5	1
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0009889	regulation of biosynthetic process	4	1
GO:0010468	regulation of gene expression	5	1
GO:0010556	regulation of macromolecule biosynthetic process	5	1
GO:0016310	phosphorylation	5	1
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0019538	protein metabolic process	3	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031326	regulation of cellular biosynthetic process	5	1
GO:0036211	protein modification process	4	1
GO:0043412	macromolecule modification	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0051171	regulation of nitrogen compound metabolic process	4	1
GO:0051252	regulation of RNA metabolic process	5	1
GO:0060255	regulation of macromolecule metabolic process	4	1
GO:0065007	biological regulation	1	1
GO:0070816	obsolete phosphorylation of RNA polymerase II C-terminal domain	6	1
GO:0080090	regulation of primary metabolic process	4	1
GO:1901564	organonitrogen compound metabolic process	3	1
GO:1903506	regulation of nucleic acid-templated transcription	7	1
GO:2001141	regulation of RNA biosynthetic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0001671	ATPase activator activity	3	12
GO:0098772	molecular function regulator activity	1	12
GO:0140677	molecular function activator activity	2	12
GO:0003676	nucleic acid binding	3	1
GO:0003677	DNA binding	4	1
GO:0003690	double-stranded DNA binding	5	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	474	478	PF00656	0.636
CLV_NRD_NRD_1	309	311	PF00675	0.354
CLV_NRD_NRD_1	352	354	PF00675	0.268
CLV_NRD_NRD_1	408	410	PF00675	0.347
CLV_NRD_NRD_1	431	433	PF00675	0.632
CLV_NRD_NRD_1	462	464	PF00675	0.612
CLV_NRD_NRD_1	48	50	PF00675	0.409
CLV_PCSK_KEX2_1	160	162	PF00082	0.493
CLV_PCSK_KEX2_1	352	354	PF00082	0.268
CLV_PCSK_KEX2_1	431	433	PF00082	0.587
CLV_PCSK_KEX2_1	462	464	PF00082	0.615
CLV_PCSK_KEX2_1	48	50	PF00082	0.406
CLV_PCSK_PC1ET2_1	160	162	PF00082	0.493
CLV_PCSK_SKI1_1	118	122	PF00082	0.489
CLV_PCSK_SKI1_1	160	164	PF00082	0.360
CLV_PCSK_SKI1_1	186	190	PF00082	0.378
CLV_PCSK_SKI1_1	207	211	PF00082	0.312
CLV_PCSK_SKI1_1	22	26	PF00082	0.384
CLV_PCSK_SKI1_1	235	239	PF00082	0.464
CLV_PCSK_SKI1_1	258	262	PF00082	0.320
CLV_PCSK_SKI1_1	410	414	PF00082	0.374
CLV_PCSK_SKI1_1	501	505	PF00082	0.433
CLV_PCSK_SKI1_1	520	524	PF00082	0.289
DEG_APCC_DBOX_1	117	125	PF00400	0.488
DOC_ANK_TNKS_1	462	469	PF00023	0.433
DOC_CKS1_1	196	201	PF01111	0.280
DOC_CYCLIN_RxL_1	113	122	PF00134	0.426
DOC_CYCLIN_RxL_1	232	240	PF00134	0.346
DOC_MAPK_FxFP_2	278	281	PF00069	0.350
DOC_PP1_RVXF_1	256	263	PF00149	0.396
DOC_PP1_RVXF_1	499	505	PF00149	0.419
DOC_PP2B_LxvP_1	553	556	PF13499	0.424
DOC_PP2B_LxvP_1	8	11	PF13499	0.460
DOC_PP4_FxxP_1	132	135	PF00568	0.588
DOC_PP4_FxxP_1	278	281	PF00568	0.350
DOC_PP4_FxxP_1	457	460	PF00568	0.476
DOC_USP7_MATH_1	425	429	PF00917	0.268
DOC_USP7_MATH_1	443	447	PF00917	0.571
DOC_USP7_MATH_1	68	72	PF00917	0.411
DOC_WW_Pin1_4	137	142	PF00397	0.466
DOC_WW_Pin1_4	195	200	PF00397	0.300
DOC_WW_Pin1_4	207	212	PF00397	0.351
DOC_WW_Pin1_4	313	318	PF00397	0.261
LIG_14-3-3_CanoR_1	102	106	PF00244	0.475
LIG_14-3-3_CanoR_1	118	128	PF00244	0.519
LIG_14-3-3_CanoR_1	161	171	PF00244	0.391
LIG_14-3-3_CanoR_1	246	256	PF00244	0.388
LIG_14-3-3_CanoR_1	295	303	PF00244	0.264
LIG_14-3-3_CanoR_1	409	418	PF00244	0.280
LIG_14-3-3_CanoR_1	419	424	PF00244	0.345
LIG_14-3-3_CanoR_1	512	517	PF00244	0.470
LIG_Actin_WH2_2	149	166	PF00022	0.407
LIG_BIR_II_1	1	5	PF00653	0.644
LIG_BRCT_BRCA1_1	158	162	PF00533	0.482
LIG_BRCT_BRCA1_1	315	319	PF00533	0.276
LIG_BRCT_BRCA1_1	331	335	PF00533	0.396
LIG_BRCT_BRCA1_1	453	457	PF00533	0.688
LIG_BRCT_BRCA1_1	72	76	PF00533	0.483
LIG_CaM_IQ_9	41	57	PF13499	0.445
LIG_CtBP_PxDLS_1	330	334	PF00389	0.378
LIG_FHA_1	166	172	PF00498	0.439
LIG_FHA_1	173	179	PF00498	0.440
LIG_FHA_1	19	25	PF00498	0.233
LIG_FHA_1	238	244	PF00498	0.431
LIG_FHA_1	255	261	PF00498	0.416
LIG_FHA_1	354	360	PF00498	0.418
LIG_FHA_1	471	477	PF00498	0.679
LIG_FHA_1	479	485	PF00498	0.502
LIG_FHA_1	521	527	PF00498	0.505
LIG_FHA_2	138	144	PF00498	0.627
LIG_FHA_2	472	478	PF00498	0.693
LIG_LIR_Apic_2	106	112	PF02991	0.519
LIG_LIR_Apic_2	454	460	PF02991	0.480
LIG_LIR_Gen_1	215	221	PF02991	0.419
LIG_LIR_Gen_1	539	550	PF02991	0.358
LIG_LIR_Gen_1	73	82	PF02991	0.496
LIG_LIR_Nem_3	215	219	PF02991	0.419
LIG_LIR_Nem_3	362	368	PF02991	0.290
LIG_LIR_Nem_3	539	545	PF02991	0.384
LIG_LIR_Nem_3	547	553	PF02991	0.391
LIG_LIR_Nem_3	73	79	PF02991	0.437
LIG_LIR_Nem_3	95	101	PF02991	0.455
LIG_LYPXL_S_1	302	306	PF13949	0.268
LIG_LYPXL_yS_3	303	306	PF13949	0.268
LIG_NRBOX	260	266	PF00104	0.268
LIG_NRBOX	62	68	PF00104	0.469
LIG_PCNA_PIPBox_1	519	528	PF02747	0.509
LIG_PCNA_yPIPBox_3	181	189	PF02747	0.452
LIG_PCNA_yPIPBox_3	224	237	PF02747	0.328
LIG_PCNA_yPIPBox_3	512	526	PF02747	0.511
LIG_SH2_CRK	542	546	PF00017	0.384
LIG_SH2_SRC	37	40	PF00017	0.444
LIG_SH2_STAP1	370	374	PF00017	0.378
LIG_SH2_STAT5	276	279	PF00017	0.350
LIG_SH2_STAT5	370	373	PF00017	0.252
LIG_SH2_STAT5	525	528	PF00017	0.510
LIG_SH3_3	143	149	PF00018	0.474
LIG_SH3_3	193	199	PF00018	0.346
LIG_SH3_3	253	259	PF00018	0.378
LIG_SH3_3	311	317	PF00018	0.303
LIG_SUMO_SIM_anti_2	234	240	PF11976	0.323
LIG_SUMO_SIM_par_1	148	153	PF11976	0.519
LIG_SUMO_SIM_par_1	234	240	PF11976	0.312
LIG_SUMO_SIM_par_1	263	269	PF11976	0.328
LIG_SUMO_SIM_par_1	328	334	PF11976	0.378
LIG_SUMO_SIM_par_1	64	71	PF11976	0.483
LIG_TRFH_1	145	149	PF08558	0.541
LIG_TRFH_1	313	317	PF08558	0.264
LIG_TYR_ITIM	301	306	PF00017	0.312
LIG_TYR_ITIM	35	40	PF00017	0.387
MOD_CK1_1	18	24	PF00069	0.350
MOD_CK1_1	2	8	PF00069	0.642
MOD_CK1_1	251	257	PF00069	0.390
MOD_CK1_1	439	445	PF00069	0.635
MOD_CK1_1	453	459	PF00069	0.647
MOD_CK2_1	137	143	PF00069	0.464
MOD_CK2_1	84	90	PF00069	0.608
MOD_Cter_Amidation	46	49	PF01082	0.420
MOD_GlcNHglycan	250	253	PF01048	0.318
MOD_GlcNHglycan	327	330	PF01048	0.341
MOD_GlcNHglycan	343	346	PF01048	0.403
MOD_GlcNHglycan	437	441	PF01048	0.601
MOD_GlcNHglycan	446	449	PF01048	0.603
MOD_GlcNHglycan	457	460	PF01048	0.597
MOD_GlcNHglycan	5	8	PF01048	0.537
MOD_GSK3_1	246	253	PF00069	0.431
MOD_GSK3_1	266	273	PF00069	0.170
MOD_GSK3_1	325	332	PF00069	0.298
MOD_GSK3_1	439	446	PF00069	0.607
MOD_GSK3_1	449	456	PF00069	0.618
MOD_GSK3_1	466	473	PF00069	0.471
MOD_GSK3_1	80	87	PF00069	0.487
MOD_GSK3_1	88	95	PF00069	0.625
MOD_N-GLC_1	246	251	PF02516	0.310
MOD_NEK2_1	1	6	PF00069	0.619
MOD_NEK2_1	156	161	PF00069	0.407
MOD_NEK2_1	162	167	PF00069	0.380
MOD_NEK2_1	237	242	PF00069	0.297
MOD_NEK2_1	331	336	PF00069	0.370
MOD_NEK2_1	339	344	PF00069	0.309
MOD_NEK2_1	455	460	PF00069	0.734
MOD_NEK2_1	54	59	PF00069	0.522
MOD_NEK2_1	82	87	PF00069	0.629
MOD_NEK2_1	88	93	PF00069	0.637
MOD_PIKK_1	119	125	PF00454	0.376
MOD_PIKK_1	80	86	PF00454	0.620
MOD_PIKK_1	92	98	PF00454	0.402
MOD_PKA_2	101	107	PF00069	0.469
MOD_PKA_2	294	300	PF00069	0.264
MOD_PKA_2	397	403	PF00069	0.276
MOD_PKA_2	54	60	PF00069	0.594
MOD_Plk_4	266	272	PF00069	0.420
MOD_Plk_4	355	361	PF00069	0.252
MOD_Plk_4	425	431	PF00069	0.415
MOD_Plk_4	478	484	PF00069	0.493
MOD_ProDKin_1	137	143	PF00069	0.464
MOD_ProDKin_1	195	201	PF00069	0.300
MOD_ProDKin_1	207	213	PF00069	0.351
MOD_ProDKin_1	313	319	PF00069	0.261
MOD_SUMO_rev_2	307	313	PF00179	0.280
TRG_DiLeu_BaEn_1	234	239	PF01217	0.370
TRG_DiLeu_BaEn_1	28	33	PF01217	0.367
TRG_DiLeu_BaLyEn_6	256	261	PF01217	0.378
TRG_DiLeu_BaLyEn_6	301	306	PF01217	0.274
TRG_DiLeu_BaLyEn_6	553	558	PF01217	0.369
TRG_ENDOCYTIC_2	303	306	PF00928	0.275
TRG_ENDOCYTIC_2	37	40	PF00928	0.335
TRG_ENDOCYTIC_2	542	545	PF00928	0.326
TRG_ER_diArg_1	351	353	PF00400	0.271
TRG_ER_diArg_1	430	432	PF00400	0.628
TRG_Pf-PMV_PEXEL_1	118	123	PF00026	0.490

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7V5	Leptomonas seymouri	65%	100%
A0A0S4JS94	Bodo saltans	31%	100%
A0A1X0P966	Trypanosomatidae	41%	100%
A0A3S5H816	Leishmania donovani	92%	100%
A0A422NK89	Trypanosoma rangeli	43%	100%
A4HNU0	Leishmania braziliensis	82%	100%
A4ICQ4	Leishmania infantum	91%	100%
D0A2Q5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
Q02939	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	22%	100%
Q4Q1Z6	Leishmania major	91%	100%
V5B9Y1	Trypanosoma cruzi	42%	100%