Ribonuclease

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Ribonuclease

Gene product:

ribonuclease H, putative

Species:

Leishmania mexicana

UniProt:

E9ASI4_LEIMU

TriTrypDb:

LmxM.36.0640

Length:

316

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0032299	ribonuclease H2 complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	1
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1

Expansion

Sequence features

E9ASI4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASI4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006259	DNA metabolic process	4	1
GO:0006281	DNA repair	5	1
GO:0006298	mismatch repair	6	1
GO:0006401	RNA catabolic process	5	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0006950	response to stress	2	1
GO:0006974	DNA damage response	4	1
GO:0008152	metabolic process	1	1
GO:0009056	catabolic process	2	1
GO:0009057	macromolecule catabolic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0019439	aromatic compound catabolic process	4	1
GO:0033554	cellular response to stress	3	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034655	nucleobase-containing compound catabolic process	4	1
GO:0043137	DNA replication, removal of RNA primer	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044248	cellular catabolic process	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0044265	obsolete cellular macromolecule catabolic process	4	1
GO:0044270	cellular nitrogen compound catabolic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0046700	heterocycle catabolic process	4	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901361	organic cyclic compound catabolic process	4	1
GO:1901575	organic substance catabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004518	nuclease activity	4	11
GO:0004519	endonuclease activity	5	11
GO:0004521	RNA endonuclease activity	5	11
GO:0004523	RNA-DNA hybrid ribonuclease activity	7	11
GO:0004540	RNA nuclease activity	4	11
GO:0005488	binding	1	11
GO:0016787	hydrolase activity	2	11
GO:0016788	hydrolase activity, acting on ester bonds	3	11
GO:0016891	RNA endonuclease activity, producing 5'-phosphomonoesters	6	11
GO:0016893	endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	11
GO:0043167	ion binding	2	11
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	202	206	PF00656	0.350
CLV_NRD_NRD_1	8	10	PF00675	0.509
CLV_NRD_NRD_1	84	86	PF00675	0.259
CLV_PCSK_KEX2_1	20	22	PF00082	0.624
CLV_PCSK_KEX2_1	297	299	PF00082	0.534
CLV_PCSK_KEX2_1	8	10	PF00082	0.681
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.607
CLV_PCSK_PC1ET2_1	297	299	PF00082	0.646
CLV_PCSK_SKI1_1	101	105	PF00082	0.275
CLV_PCSK_SKI1_1	107	111	PF00082	0.270
CLV_Separin_Metazoa	75	79	PF03568	0.350
DEG_APCC_DBOX_1	90	98	PF00400	0.559
DOC_ANK_TNKS_1	53	60	PF00023	0.507
DOC_MAPK_gen_1	104	113	PF00069	0.486
DOC_MAPK_gen_1	20	29	PF00069	0.523
DOC_MAPK_MEF2A_6	174	182	PF00069	0.455
DOC_MAPK_MEF2A_6	247	256	PF00069	0.537
DOC_USP7_MATH_1	10	14	PF00917	0.672
DOC_USP7_MATH_1	191	195	PF00917	0.463
DOC_USP7_MATH_1	242	246	PF00917	0.523
DOC_USP7_MATH_1	257	261	PF00917	0.349
DOC_USP7_UBL2_3	185	189	PF12436	0.394
DOC_USP7_UBL2_3	293	297	PF12436	0.673
DOC_WW_Pin1_4	1	6	PF00397	0.735
DOC_WW_Pin1_4	228	233	PF00397	0.350
LIG_14-3-3_CanoR_1	101	107	PF00244	0.461
LIG_14-3-3_CanoR_1	179	183	PF00244	0.455
LIG_14-3-3_CanoR_1	247	251	PF00244	0.412
LIG_14-3-3_CanoR_1	298	302	PF00244	0.445
LIG_14-3-3_CanoR_1	93	98	PF00244	0.492
LIG_DLG_GKlike_1	93	100	PF00625	0.535
LIG_EH1_1	28	36	PF00400	0.551
LIG_FHA_1	108	114	PF00498	0.459
LIG_FHA_1	301	307	PF00498	0.478
LIG_FHA_1	310	316	PF00498	0.501
LIG_FHA_1	62	68	PF00498	0.535
LIG_FHA_2	200	206	PF00498	0.535
LIG_Integrin_isoDGR_2	126	128	PF01839	0.150
LIG_LIR_Gen_1	108	113	PF02991	0.370
LIG_LIR_Gen_1	300	310	PF02991	0.375
LIG_LIR_Gen_1	41	52	PF02991	0.457
LIG_LIR_Nem_3	105	109	PF02991	0.455
LIG_LIR_Nem_3	187	193	PF02991	0.527
LIG_LIR_Nem_3	240	246	PF02991	0.415
LIG_LIR_Nem_3	249	253	PF02991	0.504
LIG_LIR_Nem_3	300	305	PF02991	0.386
LIG_LIR_Nem_3	41	47	PF02991	0.468
LIG_RPA_C_Fungi	199	211	PF08784	0.161
LIG_SH2_CRK	243	247	PF00017	0.403
LIG_SH2_STAP1	302	306	PF00017	0.497
LIG_SH2_STAT5	157	160	PF00017	0.308
LIG_SH2_STAT5	219	222	PF00017	0.161
LIG_SH2_STAT5	302	305	PF00017	0.506
LIG_SH2_STAT5	44	47	PF00017	0.448
LIG_SH2_STAT5	63	66	PF00017	0.141
LIG_SH3_3	158	164	PF00018	0.271
LIG_SH3_3	259	265	PF00018	0.482
LIG_SH3_3	54	60	PF00018	0.328
LIG_SH3_4	293	300	PF00018	0.665
LIG_SUMO_SIM_par_1	66	72	PF11976	0.308
LIG_TRAF2_1	114	117	PF00917	0.420
LIG_WRC_WIRS_1	103	108	PF05994	0.308
MOD_CDK_SPxxK_3	1	8	PF00069	0.475
MOD_CDK_SPxxK_3	228	235	PF00069	0.161
MOD_CK1_1	134	140	PF00069	0.306
MOD_CK1_1	143	149	PF00069	0.308
MOD_CK1_1	194	200	PF00069	0.420
MOD_CK2_1	111	117	PF00069	0.308
MOD_CK2_1	22	28	PF00069	0.542
MOD_CK2_1	66	72	PF00069	0.413
MOD_GlcNHglycan	12	15	PF01048	0.620
MOD_GlcNHglycan	148	151	PF01048	0.381
MOD_GlcNHglycan	16	19	PF01048	0.572
MOD_GlcNHglycan	193	196	PF01048	0.420
MOD_GlcNHglycan	216	219	PF01048	0.271
MOD_GlcNHglycan	259	262	PF01048	0.292
MOD_GlcNHglycan	40	43	PF01048	0.339
MOD_GlcNHglycan	5	8	PF01048	0.629
MOD_GSK3_1	10	17	PF00069	0.618
MOD_GSK3_1	107	114	PF00069	0.308
MOD_GSK3_1	242	249	PF00069	0.364
MOD_GSK3_1	34	41	PF00069	0.370
MOD_N-GLC_1	257	262	PF02516	0.277
MOD_N-GLC_1	287	292	PF02516	0.475
MOD_NEK2_1	16	21	PF00069	0.545
MOD_NEK2_1	178	183	PF00069	0.308
MOD_NEK2_1	237	242	PF00069	0.161
MOD_NEK2_1	61	66	PF00069	0.271
MOD_NEK2_2	242	247	PF00069	0.161
MOD_PIKK_1	273	279	PF00454	0.461
MOD_PKA_1	297	303	PF00069	0.374
MOD_PKA_2	10	16	PF00069	0.670
MOD_PKA_2	178	184	PF00069	0.335
MOD_PKA_2	22	28	PF00069	0.540
MOD_PKA_2	246	252	PF00069	0.308
MOD_PKA_2	297	303	PF00069	0.373
MOD_PKA_2	53	59	PF00069	0.278
MOD_PKB_1	91	99	PF00069	0.420
MOD_Plk_1	143	149	PF00069	0.414
MOD_Plk_1	257	263	PF00069	0.271
MOD_Plk_1	27	33	PF00069	0.352
MOD_Plk_1	287	293	PF00069	0.473
MOD_Plk_4	194	200	PF00069	0.296
MOD_Plk_4	22	28	PF00069	0.665
MOD_Plk_4	297	303	PF00069	0.437
MOD_Plk_4	53	59	PF00069	0.422
MOD_ProDKin_1	1	7	PF00069	0.732
MOD_ProDKin_1	228	234	PF00069	0.161
TRG_DiLeu_BaEn_1	72	77	PF01217	0.308
TRG_ENDOCYTIC_2	243	246	PF00928	0.403
TRG_ENDOCYTIC_2	302	305	PF00928	0.506
TRG_ENDOCYTIC_2	44	47	PF00928	0.461
TRG_ER_diArg_1	8	11	PF00400	0.511
TRG_ER_diArg_1	90	93	PF00400	0.420

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7W5	Leptomonas seymouri	74%	99%
A0A1X0P866	Trypanosomatidae	56%	96%
A0A3R7RS79	Trypanosoma rangeli	58%	98%
A0A3S7XAC0	Leishmania donovani	77%	100%
A0B796	Methanothrix thermoacetophila (strain DSM 6194 / JCM 14653 / NBRC 101360 / PT)	27%	100%
A2BL34	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	39%	100%
A4FWE5	Methanococcus maripaludis (strain C5 / ATCC BAA-1333)	30%	100%
A4HNS4	Leishmania braziliensis	79%	100%
A4ICS0	Leishmania infantum	78%	100%
A6VGW0	Methanococcus maripaludis (strain C7 / ATCC BAA-1331)	29%	100%
A9A9T9	Methanococcus maripaludis (strain C6 / ATCC BAA-1332)	29%	100%
D0A2N4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	56%	97%
O59351	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	33%	100%
O74035	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	32%	100%
O75792	Homo sapiens	42%	100%
P53942	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	35%	100%
Q0W5R8	Methanocella arvoryzae (strain DSM 22066 / NBRC 105507 / MRE50)	31%	100%
Q10236	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	37%	97%
Q2NEI6	Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3)	30%	100%
Q2TBT5	Bos taurus	43%	100%
Q3IRF6	Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara)	30%	100%
Q4JA60	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	28%	100%
Q54D13	Dictyostelium discoideum	32%	100%
Q5U209	Rattus norvegicus	42%	100%
Q8ISS8	Leishmania major	83%	100%
Q8PTA3	Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)	29%	100%
Q8TPF4	Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)	29%	100%
Q8U036	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	31%	100%
Q9CWY8	Mus musculus	41%	100%
Q9P5X8	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	34%	100%
Q9SEZ6	Arabidopsis thaliana	37%	100%
Q9U6P6	Caenorhabditis elegans	36%	100%
Q9V1A9	Pyrococcus abyssi (strain GE5 / Orsay)	31%	100%
Q9VPP5	Drosophila melanogaster	35%	91%
Q9YET5	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	31%	100%
V5BQG7	Trypanosoma cruzi	56%	98%