Dihydrouridine synthase domain protein-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

dihydrouridine synthase domain protein-like protein

Species:

Leishmania mexicana

UniProt:

E9ASG4_LEIMU

TriTrypDb:

LmxM.36.0470

Length:

440

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9ASG4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASG4

Function

Biological processes
Term	Name	Level	Count
GO:0002943	tRNA dihydrouridine synthesis	7	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	11
GO:0016491	oxidoreductase activity	2	11
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	11
GO:0017150	tRNA dihydrouridine synthase activity	5	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0050660	flavin adenine dinucleotide binding	4	11
GO:0097159	organic cyclic compound binding	2	11
GO:0106413	dihydrouridine synthase activity	4	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	174	178	PF00656	0.350
CLV_C14_Caspase3-7	268	272	PF00656	0.539
CLV_C14_Caspase3-7	303	307	PF00656	0.350
CLV_NRD_NRD_1	95	97	PF00675	0.410
CLV_PCSK_KEX2_1	154	156	PF00082	0.358
CLV_PCSK_KEX2_1	182	184	PF00082	0.270
CLV_PCSK_KEX2_1	410	412	PF00082	0.713
CLV_PCSK_KEX2_1	428	430	PF00082	0.450
CLV_PCSK_KEX2_1	78	80	PF00082	0.375
CLV_PCSK_PC1ET2_1	154	156	PF00082	0.358
CLV_PCSK_PC1ET2_1	182	184	PF00082	0.303
CLV_PCSK_PC1ET2_1	410	412	PF00082	0.713
CLV_PCSK_PC1ET2_1	428	430	PF00082	0.450
CLV_PCSK_PC1ET2_1	78	80	PF00082	0.376
CLV_PCSK_SKI1_1	105	109	PF00082	0.331
CLV_PCSK_SKI1_1	11	15	PF00082	0.317
CLV_PCSK_SKI1_1	204	208	PF00082	0.334
CLV_PCSK_SKI1_1	275	279	PF00082	0.345
CLV_PCSK_SKI1_1	288	292	PF00082	0.284
CLV_PCSK_SKI1_1	330	334	PF00082	0.376
CLV_PCSK_SKI1_1	340	344	PF00082	0.378
CLV_PCSK_SKI1_1	358	362	PF00082	0.547
CLV_Separin_Metazoa	244	248	PF03568	0.558
DEG_Nend_UBRbox_1	1	4	PF02207	0.458
DEG_SCF_FBW7_2	60	66	PF00400	0.524
DEG_SPOP_SBC_1	432	436	PF00917	0.540
DOC_CKS1_1	211	216	PF01111	0.531
DOC_CKS1_1	60	65	PF01111	0.529
DOC_CKS1_1	83	88	PF01111	0.409
DOC_CYCLIN_RxL_1	327	336	PF00134	0.420
DOC_CYCLIN_yCln2_LP_2	83	89	PF00134	0.458
DOC_MAPK_gen_1	179	188	PF00069	0.458
DOC_MAPK_HePTP_8	176	188	PF00069	0.525
DOC_MAPK_HePTP_8	8	20	PF00069	0.204
DOC_MAPK_MEF2A_6	100	108	PF00069	0.490
DOC_MAPK_MEF2A_6	11	20	PF00069	0.329
DOC_MAPK_MEF2A_6	179	188	PF00069	0.438
DOC_MAPK_NFAT4_5	105	113	PF00069	0.539
DOC_PP1_RVXF_1	328	335	PF00149	0.477
DOC_USP7_MATH_1	169	173	PF00917	0.545
DOC_USP7_MATH_1	305	309	PF00917	0.508
DOC_USP7_MATH_1	416	420	PF00917	0.731
DOC_USP7_UBL2_3	189	193	PF12436	0.497
DOC_USP7_UBL2_3	74	78	PF12436	0.408
DOC_WW_Pin1_4	19	24	PF00397	0.494
DOC_WW_Pin1_4	210	215	PF00397	0.532
DOC_WW_Pin1_4	225	230	PF00397	0.408
DOC_WW_Pin1_4	405	410	PF00397	0.434
DOC_WW_Pin1_4	59	64	PF00397	0.518
DOC_WW_Pin1_4	82	87	PF00397	0.396
LIG_14-3-3_CanoR_1	183	189	PF00244	0.460
LIG_14-3-3_CanoR_1	204	214	PF00244	0.462
LIG_14-3-3_CanoR_1	338	343	PF00244	0.473
LIG_14-3-3_CanoR_1	372	381	PF00244	0.225
LIG_14-3-3_CanoR_1	382	386	PF00244	0.440
LIG_AP2alpha_2	71	73	PF02296	0.480
LIG_APCC_ABBA_1	360	365	PF00400	0.460
LIG_BIR_III_4	177	181	PF00653	0.350
LIG_BRCT_BRCA1_1	21	25	PF00533	0.474
LIG_FHA_1	37	43	PF00498	0.415
LIG_FHA_1	395	401	PF00498	0.600
LIG_FHA_1	78	84	PF00498	0.482
LIG_FHA_2	382	388	PF00498	0.516
LIG_GBD_Chelix_1	184	192	PF00786	0.339
LIG_LIR_Gen_1	341	350	PF02991	0.413
LIG_LIR_Gen_1	375	385	PF02991	0.493
LIG_LIR_Nem_3	22	28	PF02991	0.452
LIG_LIR_Nem_3	331	337	PF02991	0.370
LIG_LIR_Nem_3	341	346	PF02991	0.375
LIG_LIR_Nem_3	351	357	PF02991	0.441
LIG_LIR_Nem_3	375	381	PF02991	0.452
LIG_LIR_Nem_3	66	70	PF02991	0.348
LIG_LYPXL_yS_3	57	60	PF13949	0.504
LIG_PCNA_PIPBox_1	326	335	PF02747	0.458
LIG_PTB_Apo_2	342	349	PF02174	0.471
LIG_PTB_Phospho_1	342	348	PF10480	0.475
LIG_REV1ctd_RIR_1	378	386	PF16727	0.427
LIG_SH2_CRK	339	343	PF00017	0.385
LIG_SH2_NCK_1	239	243	PF00017	0.465
LIG_SH2_STAP1	239	243	PF00017	0.465
LIG_SH2_STAP1	324	328	PF00017	0.488
LIG_SH2_STAP1	335	339	PF00017	0.465
LIG_SH2_STAP1	374	378	PF00017	0.377
LIG_SH2_STAT5	374	377	PF00017	0.334
LIG_SH2_STAT5	67	70	PF00017	0.410
LIG_SH3_3	109	115	PF00018	0.465
LIG_SH3_3	17	23	PF00018	0.466
LIG_SH3_3	211	217	PF00018	0.538
LIG_SH3_3	52	58	PF00018	0.511
LIG_SH3_3	83	89	PF00018	0.500
LIG_SUMO_SIM_par_1	79	85	PF11976	0.435
LIG_TRAF2_1	384	387	PF00917	0.382
LIG_TRAF2_1	418	421	PF00917	0.717
LIG_TYR_ITIM	337	342	PF00017	0.471
LIG_TYR_ITIM	55	60	PF00017	0.508
MOD_CDK_SPK_2	405	410	PF00069	0.380
MOD_CDK_SPxK_1	405	411	PF00069	0.391
MOD_CDK_SPxxK_3	19	26	PF00069	0.481
MOD_CDK_SPxxK_3	225	232	PF00069	0.539
MOD_CK1_1	187	193	PF00069	0.470
MOD_CK1_1	265	271	PF00069	0.465
MOD_CK2_1	381	387	PF00069	0.456
MOD_Cter_Amidation	180	183	PF01082	0.296
MOD_Cter_Amidation	94	97	PF01082	0.410
MOD_DYRK1A_RPxSP_1	19	23	PF00069	0.488
MOD_GSK3_1	192	199	PF00069	0.455
MOD_GSK3_1	381	388	PF00069	0.230
MOD_GSK3_1	390	397	PF00069	0.530
MOD_GSK3_1	412	419	PF00069	0.715
MOD_GSK3_1	432	439	PF00069	0.722
MOD_GSK3_1	73	80	PF00069	0.443
MOD_N-GLC_1	262	267	PF02516	0.253
MOD_NEK2_1	262	267	PF00069	0.463
MOD_NEK2_1	333	338	PF00069	0.462
MOD_NEK2_1	390	395	PF00069	0.438
MOD_NEK2_1	73	78	PF00069	0.536
MOD_PK_1	140	146	PF00069	0.453
MOD_PKA_1	140	146	PF00069	0.453
MOD_PKA_2	381	387	PF00069	0.427
MOD_PKB_1	429	437	PF00069	0.447
MOD_Plk_1	196	202	PF00069	0.539
MOD_Plk_1	3	9	PF00069	0.494
MOD_Plk_1	305	311	PF00069	0.399
MOD_Plk_1	385	391	PF00069	0.234
MOD_Plk_2-3	370	376	PF00069	0.500
MOD_Plk_2-3	66	72	PF00069	0.424
MOD_Plk_4	262	268	PF00069	0.507
MOD_Plk_4	3	9	PF00069	0.424
MOD_ProDKin_1	19	25	PF00069	0.492
MOD_ProDKin_1	210	216	PF00069	0.532
MOD_ProDKin_1	225	231	PF00069	0.408
MOD_ProDKin_1	405	411	PF00069	0.445
MOD_ProDKin_1	59	65	PF00069	0.511
MOD_ProDKin_1	82	88	PF00069	0.400
MOD_SUMO_for_1	1	4	PF00179	0.477
MOD_SUMO_rev_2	268	277	PF00179	0.514
TRG_DiLeu_BaEn_1	256	261	PF01217	0.433
TRG_ENDOCYTIC_2	339	342	PF00928	0.400
TRG_ENDOCYTIC_2	378	381	PF00928	0.392
TRG_ENDOCYTIC_2	57	60	PF00928	0.504
TRG_ENDOCYTIC_2	70	73	PF00928	0.415
TRG_ER_diArg_1	429	432	PF00400	0.738
TRG_NLS_Bipartite_1	410	432	PF00514	0.454
TRG_NLS_MonoExtC_3	427	433	PF00514	0.469

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Z1	Leptomonas seymouri	65%	100%
A0A0N1PBT7	Leptomonas seymouri	24%	86%
A0A0S4JLG1	Bodo saltans	36%	88%
A0A1X0P8K8	Trypanosomatidae	44%	100%
A0A3Q8IJ00	Leishmania donovani	90%	100%
A0A3Q8IN46	Leishmania donovani	24%	89%
A0A3R7KZP3	Trypanosoma rangeli	24%	100%
A0A422P287	Trypanosoma rangeli	48%	100%
A3LUK5	Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545)	23%	72%
A4HNQ5	Leishmania braziliensis	79%	100%
A4I9S4	Leishmania infantum	24%	89%
A4ICU0	Leishmania infantum	90%	100%
D0A2L3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9B4T5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	89%
O74553	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
O74731	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	92%
P37567	Bacillus subtilis (strain 168)	26%	100%
P44965	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	25%	100%
P53720	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
Q4Q231	Leishmania major	90%	100%
Q4Q391	Leishmania major	25%	89%
Q8PP72	Xanthomonas axonopodis pv. citri (strain 306)	26%	100%
Q8XYX1	Ralstonia solanacearum (strain GMI1000)	28%	100%
Q8ZAX7	Yersinia pestis	27%	100%
Q9D7B1	Mus musculus	27%	89%
Q9NX74	Homo sapiens	27%	89%
V5DJT2	Trypanosoma cruzi	26%	100%
V5DRQ6	Trypanosoma cruzi	49%	100%