Phosphatidylinositol-4-phosphate 5-kinase-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

phosphatidylinositol-4-phosphate 5-kinase-like protein

Species:

Leishmania mexicana

UniProt:

E9ASF4_LEIMU

TriTrypDb:

LmxM.36.0370

Length:

455

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0005886	plasma membrane	3	1
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ASF4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASF4

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	13
GO:0006796	phosphate-containing compound metabolic process	4	13
GO:0008152	metabolic process	1	13
GO:0009987	cellular process	1	13
GO:0016310	phosphorylation	5	13
GO:0044237	cellular metabolic process	2	13
GO:0006629	lipid metabolic process	3	1
GO:0006644	phospholipid metabolic process	4	1
GO:0006650	glycerophospholipid metabolic process	5	1
GO:0006661	phosphatidylinositol biosynthetic process	6	1
GO:0008610	lipid biosynthetic process	4	1
GO:0008654	phospholipid biosynthetic process	5	1
GO:0009058	biosynthetic process	2	1
GO:0019637	organophosphate metabolic process	3	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044255	cellular lipid metabolic process	3	1
GO:0045017	glycerolipid biosynthetic process	4	1
GO:0046474	glycerophospholipid biosynthetic process	5	1
GO:0046486	glycerolipid metabolic process	4	1
GO:0046488	phosphatidylinositol metabolic process	6	1
GO:0046854	phosphatidylinositol phosphate biosynthetic process	7	1
GO:0071704	organic substance metabolic process	2	1
GO:0090407	organophosphate biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	13
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0005524	ATP binding	5	13
GO:0016301	kinase activity	4	13
GO:0016307	phosphatidylinositol phosphate kinase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	13
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	13
GO:0017076	purine nucleotide binding	4	13
GO:0030554	adenyl nucleotide binding	5	13
GO:0032553	ribonucleotide binding	3	13
GO:0032555	purine ribonucleotide binding	4	13
GO:0032559	adenyl ribonucleotide binding	5	13
GO:0035639	purine ribonucleoside triphosphate binding	4	13
GO:0036094	small molecule binding	2	13
GO:0043167	ion binding	2	13
GO:0043168	anion binding	3	13
GO:0097159	organic cyclic compound binding	2	13
GO:0097367	carbohydrate derivative binding	2	13
GO:1901265	nucleoside phosphate binding	3	13
GO:1901363	heterocyclic compound binding	2	13
GO:0016308	1-phosphatidylinositol-4-phosphate 5-kinase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	215	217	PF00675	0.198
CLV_NRD_NRD_1	238	240	PF00675	0.355
CLV_NRD_NRD_1	284	286	PF00675	0.274
CLV_NRD_NRD_1	292	294	PF00675	0.334
CLV_NRD_NRD_1	435	437	PF00675	0.257
CLV_PCSK_KEX2_1	284	286	PF00082	0.346
CLV_PCSK_KEX2_1	294	296	PF00082	0.345
CLV_PCSK_KEX2_1	333	335	PF00082	0.341
CLV_PCSK_KEX2_1	409	411	PF00082	0.432
CLV_PCSK_KEX2_1	435	437	PF00082	0.343
CLV_PCSK_PC1ET2_1	294	296	PF00082	0.394
CLV_PCSK_PC1ET2_1	333	335	PF00082	0.341
CLV_PCSK_PC1ET2_1	409	411	PF00082	0.224
CLV_PCSK_PC7_1	290	296	PF00082	0.260
CLV_PCSK_SKI1_1	12	16	PF00082	0.440
CLV_PCSK_SKI1_1	135	139	PF00082	0.369
CLV_PCSK_SKI1_1	176	180	PF00082	0.259
CLV_PCSK_SKI1_1	280	284	PF00082	0.243
CLV_PCSK_SKI1_1	416	420	PF00082	0.256
CLV_PCSK_SKI1_1	435	439	PF00082	0.246
CLV_PCSK_SKI1_1	56	60	PF00082	0.531
CLV_PCSK_SKI1_1	79	83	PF00082	0.473
CLV_PCSK_SKI1_1	97	101	PF00082	0.401
DEG_SPOP_SBC_1	350	354	PF00917	0.413
DEG_SPOP_SBC_1	36	40	PF00917	0.486
DOC_CYCLIN_RxL_1	410	421	PF00134	0.140
DOC_MAPK_gen_1	154	163	PF00069	0.205
DOC_MAPK_gen_1	173	182	PF00069	0.144
DOC_MAPK_gen_1	77	85	PF00069	0.558
DOC_MAPK_HePTP_8	170	182	PF00069	0.286
DOC_MAPK_MEF2A_6	12	21	PF00069	0.266
DOC_MAPK_MEF2A_6	173	182	PF00069	0.261
DOC_MAPK_MEF2A_6	77	85	PF00069	0.570
DOC_PP1_RVXF_1	414	421	PF00149	0.260
DOC_PP4_FxxP_1	286	289	PF00568	0.420
DOC_USP7_MATH_1	165	169	PF00917	0.377
DOC_USP7_MATH_1	351	355	PF00917	0.338
DOC_USP7_MATH_1	37	41	PF00917	0.645
DOC_USP7_MATH_1	43	47	PF00917	0.650
DOC_USP7_UBL2_3	205	209	PF12436	0.254
DOC_WW_Pin1_4	47	52	PF00397	0.359
LIG_14-3-3_CanoR_1	12	21	PF00244	0.430
LIG_14-3-3_CanoR_1	157	162	PF00244	0.328
LIG_14-3-3_CanoR_1	358	363	PF00244	0.420
LIG_14-3-3_CanoR_1	435	441	PF00244	0.387
LIG_14-3-3_CanoR_1	84	92	PF00244	0.493
LIG_Actin_WH2_1	293	310	PF00022	0.260
LIG_Actin_WH2_2	10	26	PF00022	0.409
LIG_Actin_WH2_2	294	310	PF00022	0.260
LIG_BRCT_BRCA1_1	410	414	PF00533	0.194
LIG_BRCT_BRCA1_2	410	416	PF00533	0.194
LIG_CSL_BTD_1	286	289	PF09270	0.329
LIG_DLG_GKlike_1	436	443	PF00625	0.345
LIG_FHA_1	158	164	PF00498	0.185
LIG_FHA_1	402	408	PF00498	0.420
LIG_FHA_1	439	445	PF00498	0.346
LIG_FHA_2	274	280	PF00498	0.239
LIG_FHA_2	351	357	PF00498	0.260
LIG_FHA_2	375	381	PF00498	0.394
LIG_LIR_Apic_2	109	114	PF02991	0.358
LIG_LIR_Apic_2	283	289	PF02991	0.394
LIG_LIR_Gen_1	303	312	PF02991	0.289
LIG_LIR_Gen_1	314	323	PF02991	0.118
LIG_LIR_Nem_3	303	307	PF02991	0.302
LIG_LIR_Nem_3	314	318	PF02991	0.195
LIG_LIR_Nem_3	376	381	PF02991	0.368
LIG_LIR_Nem_3	392	398	PF02991	0.218
LIG_LIR_Nem_3	438	443	PF02991	0.289
LIG_LIR_Nem_3	52	58	PF02991	0.548
LIG_Pex14_2	107	111	PF04695	0.328
LIG_Pex14_2	192	196	PF04695	0.254
LIG_REV1ctd_RIR_1	412	420	PF16727	0.311
LIG_SH2_CRK	175	179	PF00017	0.395
LIG_SH2_CRK	304	308	PF00017	0.345
LIG_SH2_CRK	315	319	PF00017	0.118
LIG_SH2_PTP2	395	398	PF00017	0.239
LIG_SH2_STAP1	159	163	PF00017	0.230
LIG_SH2_STAP1	221	225	PF00017	0.140
LIG_SH2_STAP1	315	319	PF00017	0.252
LIG_SH2_STAP1	433	437	PF00017	0.287
LIG_SH2_STAT5	159	162	PF00017	0.332
LIG_SH2_STAT5	188	191	PF00017	0.279
LIG_SH2_STAT5	395	398	PF00017	0.239
LIG_SH2_STAT5	405	408	PF00017	0.239
LIG_SUMO_SIM_par_1	397	404	PF11976	0.394
LIG_TRAF2_1	145	148	PF00917	0.345
LIG_TRAF2_2	253	258	PF00917	0.140
LIG_TYR_ITIM	393	398	PF00017	0.224
LIG_UBA3_1	137	142	PF00899	0.367
LIG_WRC_WIRS_1	385	390	PF05994	0.260
LIG_WRC_WIRS_1	437	442	PF05994	0.345
LIG_WRC_WIRS_1	92	97	PF05994	0.427
MOD_CK1_1	181	187	PF00069	0.411
MOD_CK2_1	109	115	PF00069	0.430
MOD_CK2_1	142	148	PF00069	0.293
MOD_CK2_1	224	230	PF00069	0.344
MOD_CK2_1	350	356	PF00069	0.416
MOD_CK2_1	374	380	PF00069	0.399
MOD_CK2_1	47	53	PF00069	0.589
MOD_GlcNHglycan	144	147	PF01048	0.432
MOD_GlcNHglycan	226	229	PF01048	0.265
MOD_GlcNHglycan	345	348	PF01048	0.379
MOD_GlcNHglycan	353	356	PF01048	0.423
MOD_GlcNHglycan	368	371	PF01048	0.271
MOD_GlcNHglycan	410	413	PF01048	0.194
MOD_GlcNHglycan	9	12	PF01048	0.444
MOD_GSK3_1	336	343	PF00069	0.314
MOD_GSK3_1	366	373	PF00069	0.378
MOD_GSK3_1	43	50	PF00069	0.573
MOD_GSK3_1	67	74	PF00069	0.490
MOD_LATS_1	278	284	PF00433	0.394
MOD_LATS_1	98	104	PF00433	0.224
MOD_N-GLC_1	340	345	PF02516	0.383
MOD_NEK2_1	131	136	PF00069	0.252
MOD_NEK2_1	140	145	PF00069	0.252
MOD_NEK2_1	178	183	PF00069	0.316
MOD_NEK2_1	224	229	PF00069	0.445
MOD_NEK2_1	23	28	PF00069	0.356
MOD_NEK2_1	313	318	PF00069	0.302
MOD_NEK2_1	342	347	PF00069	0.197
MOD_NEK2_1	414	419	PF00069	0.293
MOD_NEK2_1	44	49	PF00069	0.576
MOD_NMyristoyl	1	7	PF02799	0.568
MOD_PIKK_1	102	108	PF00454	0.303
MOD_PIKK_1	124	130	PF00454	0.394
MOD_PIKK_1	325	331	PF00454	0.140
MOD_PIKK_1	38	44	PF00454	0.559
MOD_PKA_1	408	414	PF00069	0.225
MOD_PKA_1	435	441	PF00069	0.251
MOD_PKA_2	435	441	PF00069	0.282
MOD_PKA_2	83	89	PF00069	0.278
MOD_Plk_1	109	115	PF00069	0.412
MOD_Plk_1	313	319	PF00069	0.239
MOD_Plk_1	370	376	PF00069	0.211
MOD_Plk_1	61	67	PF00069	0.440
MOD_Plk_2-3	109	115	PF00069	0.423
MOD_Plk_4	166	172	PF00069	0.264
MOD_Plk_4	313	319	PF00069	0.280
MOD_Plk_4	414	420	PF00069	0.254
MOD_Plk_4	67	73	PF00069	0.320
MOD_ProDKin_1	47	53	PF00069	0.354
MOD_SUMO_for_1	118	121	PF00179	0.323
MOD_SUMO_for_1	58	61	PF00179	0.387
MOD_SUMO_rev_2	417	425	PF00179	0.140
TRG_DiLeu_BaEn_1	148	153	PF01217	0.252
TRG_DiLeu_BaLyEn_6	173	178	PF01217	0.140
TRG_ENDOCYTIC_2	175	178	PF00928	0.356
TRG_ENDOCYTIC_2	221	224	PF00928	0.243
TRG_ENDOCYTIC_2	304	307	PF00928	0.317
TRG_ENDOCYTIC_2	315	318	PF00928	0.223
TRG_ENDOCYTIC_2	395	398	PF00928	0.239
TRG_ER_diArg_1	284	286	PF00400	0.394
TRG_ER_diArg_1	292	295	PF00400	0.382
TRG_ER_diArg_1	435	437	PF00400	0.265
TRG_NLS_MonoExtC_3	292	298	PF00514	0.304
TRG_NLS_MonoExtN_4	237	243	PF00514	0.162
TRG_NLS_MonoExtN_4	290	297	PF00514	0.260
TRG_Pf-PMV_PEXEL_1	294	298	PF00026	0.359

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCT9	Leptomonas seymouri	23%	83%
A0A0N1PE70	Leptomonas seymouri	55%	100%
A0A0S4IZA7	Bodo saltans	27%	99%
A0A0S4JRR3	Bodo saltans	41%	98%
A0A1X0P9B0	Trypanosomatidae	47%	100%
A0A3R7KQW9	Trypanosoma rangeli	46%	100%
A0A3S7XA92	Leishmania donovani	86%	100%
A0A422N247	Trypanosoma rangeli	23%	95%
A4HNP5	Leishmania braziliensis	64%	100%
A4ICV0	Leishmania infantum	86%	100%
C9ZM20	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	95%
D0A2K2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	100%
D3ZSI8	Rattus norvegicus	24%	83%
O13010	Sus scrofa	24%	100%
O14986	Homo sapiens	23%	84%
O60331	Homo sapiens	24%	68%
O70161	Mus musculus	24%	69%
O70172	Mus musculus	24%	100%
O88377	Rattus norvegicus	24%	100%
P48426	Homo sapiens	24%	100%
P70181	Mus musculus	24%	84%
P70182	Mus musculus	24%	83%
P78356	Homo sapiens	24%	100%
Q0P5F7	Bos taurus	24%	100%
Q17QS4	Bos taurus	27%	100%
Q4Q241	Leishmania major	84%	100%
Q5CZZ9	Rattus norvegicus	24%	84%
Q5F356	Gallus gallus	23%	100%
Q5I6B8	Rattus norvegicus	23%	66%
Q5PQ01	Xenopus laevis	25%	100%
Q5T9C9	Homo sapiens	25%	100%
Q5ZJ58	Gallus gallus	24%	84%
Q6GL14	Xenopus tropicalis	25%	100%
Q6IQE1	Danio rerio	25%	100%
Q80XI4	Mus musculus	24%	100%
Q8TBX8	Homo sapiens	25%	100%
Q99755	Homo sapiens	24%	81%
Q9R0I8	Rattus norvegicus	24%	100%
V5BA13	Trypanosoma cruzi	46%	100%
V5BV28	Trypanosoma cruzi	24%	96%