Putative PUF1

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative PUF1

Gene product:

PUF1, putative

Species:

Leishmania mexicana

UniProt:

E9ASC2_LEIMU

TriTrypDb:

LmxM.36.0050

Length:

553

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 19
NetGPI	no	yes: 0, no: 20

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	3
GO:0005737	cytoplasm	2	2

Expansion

Sequence features

E9ASC2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ASC2

Function

Biological processes
Term	Name	Level	Count
GO:0010468	regulation of gene expression	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0050789	regulation of biological process	2	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	21
GO:0003723	RNA binding	4	21
GO:0005488	binding	1	21
GO:0097159	organic cyclic compound binding	2	21
GO:1901363	heterocyclic compound binding	2	21
GO:0003729	mRNA binding	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	13	15	PF00675	0.449
CLV_NRD_NRD_1	442	444	PF00675	0.420
CLV_NRD_NRD_1	449	451	PF00675	0.458
CLV_PCSK_KEX2_1	13	15	PF00082	0.448
CLV_PCSK_KEX2_1	44	46	PF00082	0.539
CLV_PCSK_KEX2_1	442	444	PF00082	0.460
CLV_PCSK_KEX2_1	449	451	PF00082	0.508
CLV_PCSK_PC1ET2_1	44	46	PF00082	0.617
CLV_PCSK_PC7_1	445	451	PF00082	0.584
CLV_PCSK_SKI1_1	104	108	PF00082	0.398
CLV_PCSK_SKI1_1	113	117	PF00082	0.463
CLV_PCSK_SKI1_1	140	144	PF00082	0.582
CLV_PCSK_SKI1_1	224	228	PF00082	0.445
CLV_PCSK_SKI1_1	343	347	PF00082	0.360
CLV_PCSK_SKI1_1	364	368	PF00082	0.310
CLV_PCSK_SKI1_1	374	378	PF00082	0.311
CLV_PCSK_SKI1_1	389	393	PF00082	0.387
CLV_PCSK_SKI1_1	449	453	PF00082	0.605
CLV_PCSK_SKI1_1	69	73	PF00082	0.505
CLV_PCSK_SKI1_1	75	79	PF00082	0.465
DEG_APCC_DBOX_1	342	350	PF00400	0.354
DEG_APCC_DBOX_1	74	82	PF00400	0.492
DOC_CYCLIN_RxL_1	443	456	PF00134	0.411
DOC_CYCLIN_yCln2_LP_2	472	475	PF00134	0.554
DOC_PP2B_LxvP_1	381	384	PF13499	0.351
DOC_PP2B_LxvP_1	472	475	PF13499	0.716
DOC_PP4_FxxP_1	148	151	PF00568	0.354
DOC_PP4_FxxP_1	510	513	PF00568	0.712
DOC_USP7_MATH_1	122	126	PF00917	0.377
DOC_USP7_MATH_1	182	186	PF00917	0.411
DOC_USP7_MATH_1	422	426	PF00917	0.526
DOC_USP7_MATH_1	490	494	PF00917	0.649
DOC_USP7_UBL2_3	435	439	PF12436	0.407
DOC_WW_Pin1_4	225	230	PF00397	0.348
DOC_WW_Pin1_4	367	372	PF00397	0.365
DOC_WW_Pin1_4	427	432	PF00397	0.384
DOC_WW_Pin1_4	456	461	PF00397	0.672
LIG_14-3-3_CanoR_1	183	191	PF00244	0.354
LIG_Actin_WH2_2	284	299	PF00022	0.335
LIG_Actin_WH2_2	354	369	PF00022	0.249
LIG_Actin_WH2_2	413	430	PF00022	0.398
LIG_Actin_WH2_2	61	77	PF00022	0.385
LIG_AP2alpha_2	301	303	PF02296	0.258
LIG_BIR_II_1	1	5	PF00653	0.542
LIG_BIR_III_4	121	125	PF00653	0.452
LIG_BIR_III_4	70	74	PF00653	0.425
LIG_BRCT_BRCA1_1	227	231	PF00533	0.462
LIG_BRCT_BRCA1_1	26	30	PF00533	0.374
LIG_CaM_IQ_9	84	100	PF13499	0.376
LIG_EVH1_2	535	539	PF00568	0.546
LIG_FHA_1	137	143	PF00498	0.449
LIG_FHA_1	200	206	PF00498	0.459
LIG_FHA_1	283	289	PF00498	0.329
LIG_FHA_1	336	342	PF00498	0.403
LIG_FHA_2	141	147	PF00498	0.539
LIG_FHA_2	150	156	PF00498	0.451
LIG_FHA_2	192	198	PF00498	0.372
LIG_FHA_2	319	325	PF00498	0.506
LIG_FHA_2	404	410	PF00498	0.325
LIG_FHA_2	44	50	PF00498	0.548
LIG_LIR_Apic_2	146	151	PF02991	0.359
LIG_LIR_Apic_2	509	513	PF02991	0.556
LIG_LIR_Gen_1	199	209	PF02991	0.438
LIG_LIR_Gen_1	228	238	PF02991	0.501
LIG_LIR_Gen_1	249	260	PF02991	0.346
LIG_LIR_Gen_1	499	508	PF02991	0.667
LIG_LIR_Nem_3	196	201	PF02991	0.421
LIG_LIR_Nem_3	228	234	PF02991	0.469
LIG_LIR_Nem_3	249	255	PF02991	0.331
LIG_LIR_Nem_3	301	306	PF02991	0.324
LIG_LIR_Nem_3	392	398	PF02991	0.284
LIG_LIR_Nem_3	499	503	PF02991	0.638
LIG_MYND_1	471	475	PF01753	0.730
LIG_PCNA_yPIPBox_3	214	226	PF02747	0.329
LIG_PCNA_yPIPBox_3	504	515	PF02747	0.528
LIG_Pex14_2	103	107	PF04695	0.361
LIG_PTB_Apo_2	426	433	PF02174	0.350
LIG_PTB_Phospho_1	426	432	PF10480	0.355
LIG_SH2_CRK	201	205	PF00017	0.471
LIG_SH2_CRK	476	480	PF00017	0.566
LIG_SH2_GRB2like	337	340	PF00017	0.359
LIG_SH2_NCK_1	248	252	PF00017	0.386
LIG_SH2_STAP1	201	205	PF00017	0.471
LIG_SH2_STAP1	248	252	PF00017	0.441
LIG_SH2_STAP1	337	341	PF00017	0.387
LIG_SH2_STAT3	306	309	PF00017	0.283
LIG_SH2_STAT3	337	340	PF00017	0.452
LIG_SH2_STAT3	398	401	PF00017	0.249
LIG_SH2_STAT5	201	204	PF00017	0.433
LIG_SH2_STAT5	306	309	PF00017	0.291
LIG_SH2_STAT5	337	340	PF00017	0.388
LIG_SH2_STAT5	398	401	PF00017	0.252
LIG_SH2_STAT5	86	89	PF00017	0.479
LIG_SH3_3	201	207	PF00018	0.462
LIG_SH3_3	296	302	PF00018	0.271
LIG_SH3_3	468	474	PF00018	0.684
LIG_SH3_3	529	535	PF00018	0.687
LIG_SUMO_SIM_anti_2	159	167	PF11976	0.344
LIG_SUMO_SIM_par_1	159	167	PF11976	0.344
LIG_TRAF2_1	194	197	PF00917	0.294
LIG_TRAF2_1	2	5	PF00917	0.454
LIG_TRAF2_1	467	470	PF00917	0.716
LIG_UBA3_1	420	428	PF00899	0.405
LIG_WRC_WIRS_1	507	512	PF05994	0.561
MOD_CDK_SPK_2	225	230	PF00069	0.229
MOD_CDK_SPxxK_3	367	374	PF00069	0.383
MOD_CK1_1	237	243	PF00069	0.613
MOD_CK1_1	405	411	PF00069	0.332
MOD_CK1_1	438	444	PF00069	0.531
MOD_CK2_1	149	155	PF00069	0.519
MOD_CK2_1	191	197	PF00069	0.299
MOD_CK2_1	200	206	PF00069	0.355
MOD_CK2_1	318	324	PF00069	0.497
MOD_CK2_1	43	49	PF00069	0.538
MOD_GlcNHglycan	136	139	PF01048	0.496
MOD_GlcNHglycan	238	242	PF01048	0.517
MOD_GlcNHglycan	482	485	PF01048	0.763
MOD_GlcNHglycan	51	54	PF01048	0.630
MOD_GSK3_1	136	143	PF00069	0.483
MOD_GSK3_1	167	174	PF00069	0.436
MOD_GSK3_1	19	26	PF00069	0.447
MOD_GSK3_1	220	227	PF00069	0.385
MOD_GSK3_1	283	290	PF00069	0.354
MOD_GSK3_1	30	37	PF00069	0.474
MOD_GSK3_1	399	406	PF00069	0.267
MOD_N-GLC_1	122	127	PF02516	0.372
MOD_N-GLC_1	218	223	PF02516	0.394
MOD_N-GLC_1	292	297	PF02516	0.339
MOD_N-GLC_1	318	323	PF02516	0.536
MOD_N-GLC_1	335	340	PF02516	0.332
MOD_N-GLC_1	49	54	PF02516	0.628
MOD_N-GLC_1	544	549	PF02516	0.711
MOD_NEK2_1	134	139	PF00069	0.422
MOD_NEK2_1	292	297	PF00069	0.368
MOD_NEK2_1	366	371	PF00069	0.425
MOD_NEK2_1	391	396	PF00069	0.294
MOD_PIKK_1	182	188	PF00454	0.383
MOD_PIKK_1	411	417	PF00454	0.313
MOD_PIKK_1	459	465	PF00454	0.682
MOD_PKA_2	182	188	PF00069	0.383
MOD_Plk_1	218	224	PF00069	0.459
MOD_Plk_1	24	30	PF00069	0.404
MOD_Plk_1	292	298	PF00069	0.481
MOD_Plk_1	490	496	PF00069	0.734
MOD_Plk_4	200	206	PF00069	0.408
MOD_Plk_4	283	289	PF00069	0.301
MOD_Plk_4	422	428	PF00069	0.417
MOD_Plk_4	506	512	PF00069	0.605
MOD_ProDKin_1	225	231	PF00069	0.344
MOD_ProDKin_1	367	373	PF00069	0.365
MOD_ProDKin_1	427	433	PF00069	0.378
MOD_ProDKin_1	456	462	PF00069	0.679
MOD_SUMO_for_1	434	437	PF00179	0.455
MOD_SUMO_rev_2	2	8	PF00179	0.422
MOD_SUMO_rev_2	48	52	PF00179	0.653
TRG_DiLeu_BaEn_1	160	165	PF01217	0.317
TRG_DiLeu_BaLyEn_6	130	135	PF01217	0.351
TRG_DiLeu_BaLyEn_6	447	452	PF01217	0.411
TRG_DiLeu_BaLyEn_6	468	473	PF01217	0.557
TRG_ENDOCYTIC_2	201	204	PF00928	0.474
TRG_ENDOCYTIC_2	248	251	PF00928	0.314
TRG_ER_diArg_1	442	445	PF00400	0.454
TRG_ER_diArg_1	449	451	PF00400	0.506
TRG_Pf-PMV_PEXEL_1	166	171	PF00026	0.479
TRG_Pf-PMV_PEXEL_1	19	23	PF00026	0.398
TRG_Pf-PMV_PEXEL_1	374	379	PF00026	0.321
TRG_Pf-PMV_PEXEL_1	45	49	PF00026	0.627

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P473	Leptomonas seymouri	80%	99%
A0A0S4IY52	Bodo saltans	28%	78%
A0A0S4JMB8	Bodo saltans	46%	79%
A0A1X0NHM5	Trypanosomatidae	29%	98%
A0A1X0P8D8	Trypanosomatidae	46%	96%
A0A3Q8IK20	Leishmania donovani	98%	100%
A0A3R7NHA6	Trypanosoma rangeli	45%	97%
A0A422N8V0	Trypanosoma rangeli	28%	100%
A4HNL0	Leishmania braziliensis	89%	100%
A4ICY3	Leishmania infantum	98%	100%
C9ZII7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	82%
D0A2G7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	97%
E8NHJ3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9AIJ1	Leishmania braziliensis	23%	100%
E9AUS6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q274	Leishmania major	98%	100%
Q4QCQ8	Leishmania major	24%	100%
V5B1W9	Trypanosoma cruzi	29%	99%
V5BV90	Trypanosoma cruzi	46%	91%