Plastid-encoded RNA polymerase subunit alpha

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerases I and III subunit RPAC1, putative

Species:

Leishmania mexicana

UniProt:

E9AS18_LEIMU

TriTrypDb:

LmxM.19.0660

Length:

434

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12
GO:0005634	nucleus	5	1
GO:0005666	RNA polymerase III complex	4	1
GO:0005730	nucleolus	5	1
GO:0005736	RNA polymerase I complex	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	1
GO:0110165	cellular anatomical entity	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

E9AS18

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AS18

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006351	DNA-templated transcription	7	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0009058	biosynthetic process	2	1
GO:0009059	macromolecule biosynthetic process	4	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0018130	heterocycle biosynthetic process	4	1
GO:0019438	aromatic compound biosynthetic process	4	1
GO:0032774	RNA biosynthetic process	5	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034654	nucleobase-containing compound biosynthetic process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044249	cellular biosynthetic process	3	1
GO:0044271	cellular nitrogen compound biosynthetic process	4	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:0097659	nucleic acid-templated transcription	6	1
GO:1901360	organic cyclic compound metabolic process	3	1
GO:1901362	organic cyclic compound biosynthetic process	4	1
GO:1901576	organic substance biosynthetic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0001054	RNA polymerase I activity	7	12
GO:0001056	RNA polymerase III activity	7	12
GO:0003824	catalytic activity	1	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0046983	protein dimerization activity	3	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	154	158	PF00656	0.430
CLV_NRD_NRD_1	115	117	PF00675	0.472
CLV_NRD_NRD_1	136	138	PF00675	0.230
CLV_NRD_NRD_1	358	360	PF00675	0.175
CLV_NRD_NRD_1	5	7	PF00675	0.726
CLV_PCSK_KEX2_1	115	117	PF00082	0.469
CLV_PCSK_KEX2_1	136	138	PF00082	0.230
CLV_PCSK_KEX2_1	358	360	PF00082	0.175
CLV_PCSK_KEX2_1	5	7	PF00082	0.726
CLV_PCSK_KEX2_1	73	75	PF00082	0.695
CLV_PCSK_PC1ET2_1	73	75	PF00082	0.726
CLV_PCSK_PC7_1	354	360	PF00082	0.175
CLV_PCSK_SKI1_1	136	140	PF00082	0.257
CLV_PCSK_SKI1_1	199	203	PF00082	0.259
DEG_APCC_DBOX_1	167	175	PF00400	0.441
DEG_APCC_DBOX_1	198	206	PF00400	0.473
DEG_APCC_DBOX_1	275	283	PF00400	0.430
DEG_SCF_FBW7_1	215	220	PF00400	0.498
DEG_SPOP_SBC_1	38	42	PF00917	0.681
DOC_ANK_TNKS_1	103	110	PF00023	0.680
DOC_CYCLIN_RxL_1	196	206	PF00134	0.441
DOC_MAPK_gen_1	115	122	PF00069	0.416
DOC_MAPK_gen_1	276	284	PF00069	0.430
DOC_MAPK_gen_1	3	13	PF00069	0.699
DOC_MAPK_gen_1	375	385	PF00069	0.451
DOC_PIKK_1	156	164	PF02985	0.527
DOC_PP1_RVXF_1	197	204	PF00149	0.430
DOC_PP1_RVXF_1	219	226	PF00149	0.492
DOC_USP7_MATH_1	31	35	PF00917	0.711
DOC_USP7_MATH_1	47	51	PF00917	0.627
DOC_USP7_MATH_1	68	72	PF00917	0.735
DOC_USP7_MATH_1	86	90	PF00917	0.748
DOC_USP7_MATH_1	91	95	PF00917	0.735
DOC_WW_Pin1_4	125	130	PF00397	0.466
DOC_WW_Pin1_4	155	160	PF00397	0.531
DOC_WW_Pin1_4	213	218	PF00397	0.483
DOC_WW_Pin1_4	23	28	PF00397	0.641
DOC_WW_Pin1_4	292	297	PF00397	0.426
LIG_14-3-3_CanoR_1	104	108	PF00244	0.651
LIG_14-3-3_CanoR_1	119	123	PF00244	0.289
LIG_14-3-3_CanoR_1	136	146	PF00244	0.358
LIG_14-3-3_CanoR_1	53	62	PF00244	0.589
LIG_Actin_WH2_2	264	282	PF00022	0.430
LIG_Actin_WH2_2	386	402	PF00022	0.514
LIG_APCC_ABBA_1	260	265	PF00400	0.430
LIG_APCC_ABBA_1	403	408	PF00400	0.441
LIG_BIR_II_1	1	5	PF00653	0.644
LIG_BIR_III_4	206	210	PF00653	0.527
LIG_BRCT_BRCA1_1	131	135	PF00533	0.457
LIG_Clathr_ClatBox_1	202	206	PF01394	0.496
LIG_Clathr_ClatBox_1	384	388	PF01394	0.469
LIG_EVH1_2	62	66	PF00568	0.694
LIG_FHA_1	112	118	PF00498	0.481
LIG_FHA_1	138	144	PF00498	0.508
LIG_FHA_1	19	25	PF00498	0.717
LIG_FHA_1	233	239	PF00498	0.412
LIG_FHA_1	379	385	PF00498	0.483
LIG_FHA_2	304	310	PF00498	0.529
LIG_FHA_2	40	46	PF00498	0.694
LIG_LIR_Gen_1	145	153	PF02991	0.496
LIG_LIR_Gen_1	191	200	PF02991	0.464
LIG_LIR_Nem_3	132	138	PF02991	0.534
LIG_LIR_Nem_3	145	151	PF02991	0.460
LIG_LIR_Nem_3	191	195	PF02991	0.464
LIG_NRBOX	160	166	PF00104	0.527
LIG_SH2_SRC	30	33	PF00017	0.700
LIG_SH2_STAP1	219	223	PF00017	0.523
LIG_SH2_STAT3	219	222	PF00017	0.527
LIG_SH2_STAT5	183	186	PF00017	0.455
LIG_SH3_3	124	130	PF00018	0.496
LIG_SH3_3	171	177	PF00018	0.460
LIG_SH3_3	21	27	PF00018	0.673
LIG_SH3_3	223	229	PF00018	0.518
LIG_SH3_3	341	347	PF00018	0.496
LIG_SH3_3	41	47	PF00018	0.478
MOD_CK1_1	26	32	PF00069	0.622
MOD_CK1_1	295	301	PF00069	0.456
MOD_CK1_1	325	331	PF00069	0.451
MOD_CK1_1	34	40	PF00069	0.619
MOD_CK1_1	89	95	PF00069	0.644
MOD_CK2_1	303	309	PF00069	0.532
MOD_CK2_1	314	320	PF00069	0.529
MOD_GlcNHglycan	297	300	PF01048	0.255
MOD_GlcNHglycan	316	319	PF01048	0.165
MOD_GlcNHglycan	33	36	PF01048	0.594
MOD_GlcNHglycan	55	58	PF01048	0.497
MOD_GlcNHglycan	82	85	PF01048	0.702
MOD_GlcNHglycan	89	92	PF01048	0.754
MOD_GSK3_1	125	132	PF00069	0.537
MOD_GSK3_1	138	145	PF00069	0.460
MOD_GSK3_1	18	25	PF00069	0.610
MOD_GSK3_1	184	191	PF00069	0.480
MOD_GSK3_1	213	220	PF00069	0.436
MOD_GSK3_1	26	33	PF00069	0.630
MOD_GSK3_1	34	41	PF00069	0.621
MOD_GSK3_1	78	85	PF00069	0.719
MOD_GSK3_1	86	93	PF00069	0.646
MOD_N-GLC_1	125	130	PF02516	0.308
MOD_N-GLC_1	155	160	PF02516	0.255
MOD_N-GLC_1	68	73	PF02516	0.745
MOD_NEK2_1	205	210	PF00069	0.489
MOD_NEK2_1	314	319	PF00069	0.493
MOD_NEK2_1	322	327	PF00069	0.491
MOD_NEK2_2	68	73	PF00069	0.554
MOD_PKA_2	103	109	PF00069	0.686
MOD_PKA_2	118	124	PF00069	0.365
MOD_PKA_2	312	318	PF00069	0.534
MOD_Plk_1	378	384	PF00069	0.455
MOD_Plk_1	68	74	PF00069	0.556
MOD_Plk_2-3	118	124	PF00069	0.309
MOD_Plk_4	340	346	PF00069	0.514
MOD_Plk_4	91	97	PF00069	0.696
MOD_ProDKin_1	125	131	PF00069	0.466
MOD_ProDKin_1	155	161	PF00069	0.531
MOD_ProDKin_1	213	219	PF00069	0.483
MOD_ProDKin_1	23	29	PF00069	0.641
MOD_ProDKin_1	292	298	PF00069	0.426
MOD_SUMO_for_1	72	75	PF00179	0.646
MOD_SUMO_rev_2	298	307	PF00179	0.441
TRG_ENDOCYTIC_2	301	304	PF00928	0.527
TRG_ER_diArg_1	115	117	PF00400	0.474
TRG_ER_diArg_1	135	137	PF00400	0.321
TRG_ER_diArg_1	357	359	PF00400	0.375
TRG_ER_diArg_1	373	376	PF00400	0.508
TRG_ER_diArg_1	4	6	PF00400	0.580
TRG_Pf-PMV_PEXEL_1	137	142	PF00026	0.286
TRG_Pf-PMV_PEXEL_1	150	154	PF00026	0.189
TRG_Pf-PMV_PEXEL_1	199	204	PF00026	0.241
TRG_Pf-PMV_PEXEL_1	411	415	PF00026	0.513

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8L4	Leptomonas seymouri	74%	97%
A0A0S4IYI3	Bodo saltans	46%	100%
A0A1X0NZD0	Trypanosomatidae	26%	100%
A0A1X0P6H8	Trypanosomatidae	51%	100%
A0A3R7MJ28	Trypanosoma rangeli	51%	100%
A0A3S5H758	Leishmania donovani	93%	98%
A0A3S7X218	Leishmania donovani	26%	100%
A4HA24	Leishmania braziliensis	82%	100%
A4HH22	Leishmania braziliensis	25%	100%
A4HY87	Leishmania infantum	93%	98%
A4I451	Leishmania infantum	26%	100%
D0A0L6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	46%	100%
E9ADL6	Leishmania major	26%	100%
E9AM65	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
O15160	Homo sapiens	28%	100%
O94616	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
P07703	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	29%	100%
P16925	Tetrahymena thermophila	24%	100%
P37382	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	24%	100%
P52432	Mus musculus	31%	100%
Q32L22	Bos taurus	30%	100%
Q39211	Arabidopsis thaliana	27%	100%
Q39212	Arabidopsis thaliana	27%	100%
Q4QDF5	Leishmania major	90%	100%
Q54DH7	Dictyostelium discoideum	28%	100%
Q54SN4	Dictyostelium discoideum	29%	100%
V5BBX7	Trypanosoma cruzi	49%	100%