Glutamate--cysteine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

gamma-glutamylcysteine synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9ARV1_LEIMU

TriTrypDb:

LmxM.18.1660

Length:

687

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ARV1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARV1

Function

Biological processes
Term	Name	Level	Count
GO:0006518	peptide metabolic process	4	12
GO:0006575	cellular modified amino acid metabolic process	3	12
GO:0006749	glutathione metabolic process	4	12
GO:0006750	glutathione biosynthetic process	5	12
GO:0006790	sulfur compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009987	cellular process	1	12
GO:0019184	nonribosomal peptide biosynthetic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0042398	cellular modified amino acid biosynthetic process	4	12
GO:0043043	peptide biosynthetic process	5	12
GO:0043603	amide metabolic process	3	12
GO:0043604	amide biosynthetic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044272	sulfur compound biosynthetic process	4	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004357	glutamate-cysteine ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	111	115	PF00656	0.434
CLV_C14_Caspase3-7	413	417	PF00656	0.372
CLV_NRD_NRD_1	122	124	PF00675	0.300
CLV_NRD_NRD_1	189	191	PF00675	0.294
CLV_NRD_NRD_1	384	386	PF00675	0.252
CLV_NRD_NRD_1	556	558	PF00675	0.256
CLV_NRD_NRD_1	632	634	PF00675	0.325
CLV_PCSK_FUR_1	187	191	PF00082	0.294
CLV_PCSK_KEX2_1	122	124	PF00082	0.301
CLV_PCSK_KEX2_1	189	191	PF00082	0.294
CLV_PCSK_KEX2_1	384	386	PF00082	0.294
CLV_PCSK_KEX2_1	604	606	PF00082	0.293
CLV_PCSK_KEX2_1	632	634	PF00082	0.215
CLV_PCSK_PC1ET2_1	604	606	PF00082	0.456
CLV_PCSK_SKI1_1	271	275	PF00082	0.464
CLV_PCSK_SKI1_1	364	368	PF00082	0.233
CLV_PCSK_SKI1_1	373	377	PF00082	0.233
CLV_PCSK_SKI1_1	391	395	PF00082	0.233
CLV_PCSK_SKI1_1	558	562	PF00082	0.267
CLV_PCSK_SKI1_1	633	637	PF00082	0.327
CLV_Separin_Metazoa	388	392	PF03568	0.229
DEG_COP1_1	162	170	PF00400	0.311
DOC_CKS1_1	273	278	PF01111	0.310
DOC_CKS1_1	503	508	PF01111	0.233
DOC_CKS1_1	624	629	PF01111	0.254
DOC_CYCLIN_RxL_1	268	278	PF00134	0.472
DOC_CYCLIN_RxL_1	361	371	PF00134	0.327
DOC_CYCLIN_yCln2_LP_2	273	279	PF00134	0.355
DOC_MAPK_DCC_7	193	202	PF00069	0.347
DOC_MAPK_gen_1	187	196	PF00069	0.289
DOC_MAPK_gen_1	38	48	PF00069	0.463
DOC_MAPK_gen_1	394	402	PF00069	0.267
DOC_MAPK_GRA24_9	186	202	PF00069	0.352
DOC_MAPK_HePTP_8	186	198	PF00069	0.286
DOC_MAPK_MEF2A_6	189	198	PF00069	0.286
DOC_MAPK_MEF2A_6	396	404	PF00069	0.267
DOC_MAPK_MEF2A_6	430	438	PF00069	0.244
DOC_PP1_RVXF_1	492	498	PF00149	0.233
DOC_PP2B_LxvP_1	165	168	PF13499	0.309
DOC_PP4_FxxP_1	248	251	PF00568	0.394
DOC_PP4_FxxP_1	482	485	PF00568	0.327
DOC_USP7_MATH_1	254	258	PF00917	0.524
DOC_USP7_MATH_1	306	310	PF00917	0.294
DOC_USP7_MATH_1	355	359	PF00917	0.233
DOC_USP7_MATH_1	486	490	PF00917	0.297
DOC_USP7_MATH_1	573	577	PF00917	0.254
DOC_USP7_MATH_1	620	624	PF00917	0.134
DOC_WW_Pin1_4	156	161	PF00397	0.355
DOC_WW_Pin1_4	217	222	PF00397	0.422
DOC_WW_Pin1_4	272	277	PF00397	0.301
DOC_WW_Pin1_4	357	362	PF00397	0.233
DOC_WW_Pin1_4	502	507	PF00397	0.233
DOC_WW_Pin1_4	603	608	PF00397	0.379
DOC_WW_Pin1_4	623	628	PF00397	0.254
DOC_WW_Pin1_4	98	103	PF00397	0.428
LIG_14-3-3_CanoR_1	235	242	PF00244	0.571
LIG_14-3-3_CanoR_1	632	641	PF00244	0.331
LIG_Actin_WH2_2	175	191	PF00022	0.313
LIG_APCC_ABBA_1	198	203	PF00400	0.290
LIG_deltaCOP1_diTrp_1	490	497	PF00928	0.293
LIG_eIF4E_1	589	595	PF01652	0.248
LIG_FHA_1	136	142	PF00498	0.292
LIG_FHA_1	181	187	PF00498	0.286
LIG_FHA_1	236	242	PF00498	0.677
LIG_FHA_1	265	271	PF00498	0.422
LIG_FHA_1	328	334	PF00498	0.248
LIG_FHA_1	406	412	PF00498	0.304
LIG_FHA_1	518	524	PF00498	0.267
LIG_FHA_1	63	69	PF00498	0.461
LIG_FHA_1	646	652	PF00498	0.246
LIG_FHA_2	235	241	PF00498	0.505
LIG_FHA_2	462	468	PF00498	0.248
LIG_FHA_2	503	509	PF00498	0.233
LIG_FHA_2	564	570	PF00498	0.293
LIG_FHA_2	635	641	PF00498	0.419
LIG_FHA_2	68	74	PF00498	0.377
LIG_GBD_Chelix_1	651	659	PF00786	0.254
LIG_HCF-1_HBM_1	103	106	PF13415	0.356
LIG_Integrin_isoDGR_2	233	235	PF01839	0.500
LIG_LIR_Apic_2	245	251	PF02991	0.398
LIG_LIR_Apic_2	479	485	PF02991	0.379
LIG_LIR_Gen_1	138	146	PF02991	0.474
LIG_LIR_Gen_1	371	381	PF02991	0.233
LIG_LIR_Gen_1	395	404	PF02991	0.242
LIG_LIR_Gen_1	424	429	PF02991	0.297
LIG_LIR_Gen_1	474	485	PF02991	0.233
LIG_LIR_Gen_1	611	620	PF02991	0.237
LIG_LIR_Nem_3	138	143	PF02991	0.479
LIG_LIR_Nem_3	371	377	PF02991	0.233
LIG_LIR_Nem_3	395	400	PF02991	0.242
LIG_LIR_Nem_3	424	428	PF02991	0.272
LIG_LIR_Nem_3	466	472	PF02991	0.234
LIG_LIR_Nem_3	585	591	PF02991	0.247
LIG_LIR_Nem_3	611	615	PF02991	0.237
LIG_LIR_Nem_3	92	96	PF02991	0.302
LIG_Pex14_1	493	497	PF04695	0.233
LIG_Pex14_1	89	93	PF04695	0.306
LIG_Pex14_2	33	37	PF04695	0.411
LIG_PTB_Apo_2	506	513	PF02174	0.237
LIG_PTB_Apo_2	87	94	PF02174	0.339
LIG_PTB_Phospho_1	87	93	PF10480	0.342
LIG_SH2_CRK	124	128	PF00017	0.376
LIG_SH2_CRK	343	347	PF00017	0.233
LIG_SH2_CRK	397	401	PF00017	0.267
LIG_SH2_CRK	531	535	PF00017	0.286
LIG_SH2_CRK	589	593	PF00017	0.248
LIG_SH2_NCK_1	397	401	PF00017	0.280
LIG_SH2_NCK_1	531	535	PF00017	0.298
LIG_SH2_SRC	598	601	PF00017	0.178
LIG_SH2_STAP1	161	165	PF00017	0.365
LIG_SH2_STAP1	282	286	PF00017	0.338
LIG_SH2_STAP1	343	347	PF00017	0.293
LIG_SH2_STAP1	454	458	PF00017	0.267
LIG_SH2_STAT3	281	284	PF00017	0.310
LIG_SH2_STAT5	285	288	PF00017	0.423
LIG_SH2_STAT5	425	428	PF00017	0.355
LIG_SH2_STAT5	531	534	PF00017	0.233
LIG_SH2_STAT5	554	557	PF00017	0.233
LIG_SH2_STAT5	598	601	PF00017	0.364
LIG_SH2_STAT5	612	615	PF00017	0.123
LIG_SH2_STAT5	672	675	PF00017	0.374
LIG_SH3_3	138	144	PF00018	0.278
LIG_SH3_3	248	254	PF00018	0.463
LIG_SH3_3	305	311	PF00018	0.341
LIG_SH3_3	511	517	PF00018	0.327
LIG_SH3_3	99	105	PF00018	0.264
LIG_SH3_5	260	264	PF00018	0.682
LIG_SUMO_SIM_anti_2	344	350	PF11976	0.327
LIG_SUMO_SIM_anti_2	532	539	PF11976	0.327
LIG_SUMO_SIM_par_1	455	464	PF11976	0.244
LIG_SUMO_SIM_par_1	63	73	PF11976	0.469
LIG_TRAF2_1	568	571	PF00917	0.254
LIG_TRFH_1	589	593	PF08558	0.248
LIG_WRC_WIRS_1	93	98	PF05994	0.295
MOD_CDK_SPK_2	217	222	PF00069	0.229
MOD_CDK_SPxxK_3	357	364	PF00069	0.233
MOD_CK1_1	109	115	PF00069	0.343
MOD_CK1_1	155	161	PF00069	0.372
MOD_CK1_1	357	363	PF00069	0.213
MOD_CK1_1	405	411	PF00069	0.304
MOD_CK1_1	623	629	PF00069	0.307
MOD_CK1_1	645	651	PF00069	0.255
MOD_CK1_1	661	667	PF00069	0.332
MOD_CK1_1	69	75	PF00069	0.467
MOD_CK2_1	226	232	PF00069	0.488
MOD_CK2_1	234	240	PF00069	0.549
MOD_CK2_1	414	420	PF00069	0.276
MOD_CK2_1	421	427	PF00069	0.305
MOD_CK2_1	461	467	PF00069	0.245
MOD_CK2_1	502	508	PF00069	0.233
MOD_CK2_1	532	538	PF00069	0.134
MOD_CK2_1	565	571	PF00069	0.290
MOD_CK2_1	573	579	PF00069	0.206
MOD_CK2_1	67	73	PF00069	0.357
MOD_Cter_Amidation	602	605	PF01082	0.254
MOD_GlcNHglycan	324	327	PF01048	0.298
MOD_GlcNHglycan	357	360	PF01048	0.293
MOD_GlcNHglycan	431	434	PF01048	0.270
MOD_GlcNHglycan	620	623	PF01048	0.341
MOD_GSK3_1	12	19	PF00069	0.420
MOD_GSK3_1	135	142	PF00069	0.291
MOD_GSK3_1	152	159	PF00069	0.409
MOD_GSK3_1	203	210	PF00069	0.343
MOD_GSK3_1	392	399	PF00069	0.364
MOD_GSK3_1	472	479	PF00069	0.251
MOD_GSK3_1	565	572	PF00069	0.279
MOD_GSK3_1	614	621	PF00069	0.291
MOD_GSK3_1	62	69	PF00069	0.407
MOD_GSK3_1	679	686	PF00069	0.547
MOD_N-GLC_1	106	111	PF02516	0.278
MOD_N-GLC_1	172	177	PF02516	0.326
MOD_N-GLC_1	180	185	PF02516	0.274
MOD_N-GLC_1	235	240	PF02516	0.665
MOD_N-GLC_1	414	419	PF02516	0.276
MOD_N-GLC_1	421	426	PF02516	0.257
MOD_N-GLC_1	539	544	PF02516	0.234
MOD_N-GLC_2	299	301	PF02516	0.425
MOD_N-GLC_2	563	565	PF02516	0.178
MOD_NEK2_1	322	327	PF00069	0.248
MOD_NEK2_1	354	359	PF00069	0.233
MOD_NEK2_1	414	419	PF00069	0.292
MOD_NEK2_1	429	434	PF00069	0.187
MOD_NEK2_1	608	613	PF00069	0.338
MOD_NEK2_1	618	623	PF00069	0.275
MOD_NEK2_1	634	639	PF00069	0.222
MOD_NEK2_1	647	652	PF00069	0.271
MOD_NEK2_1	97	102	PF00069	0.283
MOD_NEK2_2	161	166	PF00069	0.338
MOD_NEK2_2	266	271	PF00069	0.338
MOD_PIKK_1	159	165	PF00454	0.379
MOD_PK_1	396	402	PF00069	0.267
MOD_PK_1	679	685	PF00069	0.347
MOD_PKA_2	234	240	PF00069	0.401
MOD_PKA_2	254	260	PF00069	0.379
MOD_PKA_2	573	579	PF00069	0.329
MOD_PKA_2	618	624	PF00069	0.293
MOD_PKA_2	645	651	PF00069	0.264
MOD_PKB_1	80	88	PF00069	0.220
MOD_Plk_1	106	112	PF00069	0.273
MOD_Plk_1	207	213	PF00069	0.295
MOD_Plk_1	414	420	PF00069	0.281
MOD_Plk_1	421	427	PF00069	0.253
MOD_Plk_1	62	68	PF00069	0.388
MOD_Plk_2-3	203	209	PF00069	0.366
MOD_Plk_4	136	142	PF00069	0.301
MOD_Plk_4	161	167	PF00069	0.335
MOD_Plk_4	608	614	PF00069	0.383
MOD_Plk_4	673	679	PF00069	0.335
MOD_Plk_4	92	98	PF00069	0.300
MOD_ProDKin_1	156	162	PF00069	0.345
MOD_ProDKin_1	217	223	PF00069	0.417
MOD_ProDKin_1	272	278	PF00069	0.300
MOD_ProDKin_1	357	363	PF00069	0.233
MOD_ProDKin_1	502	508	PF00069	0.233
MOD_ProDKin_1	603	609	PF00069	0.379
MOD_ProDKin_1	623	629	PF00069	0.254
MOD_ProDKin_1	98	104	PF00069	0.439
MOD_SUMO_rev_2	600	606	PF00179	0.287
TRG_DiLeu_BaEn_2	91	97	PF01217	0.302
TRG_DiLeu_BaEn_3	387	393	PF01217	0.213
TRG_DiLeu_BaLyEn_6	514	519	PF01217	0.327
TRG_DiLeu_BaLyEn_6	604	609	PF01217	0.134
TRG_ENDOCYTIC_2	124	127	PF00928	0.323
TRG_ENDOCYTIC_2	343	346	PF00928	0.233
TRG_ENDOCYTIC_2	397	400	PF00928	0.241
TRG_ENDOCYTIC_2	425	428	PF00928	0.268
TRG_ENDOCYTIC_2	454	457	PF00928	0.267
TRG_ENDOCYTIC_2	531	534	PF00928	0.283
TRG_ENDOCYTIC_2	589	592	PF00928	0.247
TRG_ENDOCYTIC_2	612	615	PF00928	0.233
TRG_ENDOCYTIC_2	672	675	PF00928	0.504
TRG_ENDOCYTIC_2	93	96	PF00928	0.297
TRG_ER_diArg_1	122	124	PF00400	0.306
TRG_ER_diArg_1	186	189	PF00400	0.280
TRG_ER_diArg_1	631	633	PF00400	0.208
TRG_ER_diArg_1	79	82	PF00400	0.337
TRG_Pf-PMV_PEXEL_1	597	602	PF00026	0.293
TRG_Pf-PMV_PEXEL_1	653	657	PF00026	0.298

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1P9N5	Leptomonas seymouri	72%	100%
A0A0S4IM75	Bodo saltans	53%	100%
A0A1X0P757	Trypanosomatidae	58%	100%
A0A3S5IRL4	Trypanosoma rangeli	60%	100%
A0A3S7WV51	Leishmania donovani	91%	100%
A4H9R8	Leishmania braziliensis	76%	100%
A4HY37	Leishmania infantum	91%	100%
D0A548	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
Q09768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	100%
Q4QDM2	Leishmania major	89%	100%
Q9HF78	Candida albicans	33%	100%
V5BDE4	Trypanosoma cruzi	59%	100%