Proline--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

prolyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9ARQ8_LEIMU

TriTrypDb:

LmxM.18.1210

Length:

727

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 28

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	16
GO:0110165	cellular anatomical entity	1	16
GO:0017101	aminoacyl-tRNA synthetase multienzyme complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0140535	intracellular protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:0016020	membrane	2	1

Expansion

Sequence features

E9ARQ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARQ8

PDB structure(s): 5xil_A

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	16
GO:0006139	nucleobase-containing compound metabolic process	3	16
GO:0006399	tRNA metabolic process	7	16
GO:0006418	tRNA aminoacylation for protein translation	6	16
GO:0006433	prolyl-tRNA aminoacylation	7	16
GO:0006520	amino acid metabolic process	3	16
GO:0006725	cellular aromatic compound metabolic process	3	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016070	RNA metabolic process	5	16
GO:0019752	carboxylic acid metabolic process	5	16
GO:0034641	cellular nitrogen compound metabolic process	3	16
GO:0034660	ncRNA metabolic process	6	16
GO:0043038	amino acid activation	4	16
GO:0043039	tRNA aminoacylation	5	16
GO:0043170	macromolecule metabolic process	3	16
GO:0043436	oxoacid metabolic process	4	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0044281	small molecule metabolic process	2	16
GO:0046483	heterocycle metabolic process	3	16
GO:0071704	organic substance metabolic process	2	16
GO:0090304	nucleic acid metabolic process	4	16
GO:1901360	organic cyclic compound metabolic process	3	16
GO:1901564	organonitrogen compound metabolic process	3	16

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0002161	aminoacyl-tRNA editing activity	5	15
GO:0003824	catalytic activity	1	16
GO:0004812	aminoacyl-tRNA ligase activity	4	16
GO:0004827	proline-tRNA ligase activity	5	16
GO:0005488	binding	1	16
GO:0005524	ATP binding	5	16
GO:0016787	hydrolase activity	2	15
GO:0016788	hydrolase activity, acting on ester bonds	3	15
GO:0016874	ligase activity	2	16
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	16
GO:0017076	purine nucleotide binding	4	16
GO:0030554	adenyl nucleotide binding	5	16
GO:0032553	ribonucleotide binding	3	16
GO:0032555	purine ribonucleotide binding	4	16
GO:0032559	adenyl ribonucleotide binding	5	16
GO:0035639	purine ribonucleoside triphosphate binding	4	16
GO:0036094	small molecule binding	2	16
GO:0043167	ion binding	2	16
GO:0043168	anion binding	3	16
GO:0052689	carboxylic ester hydrolase activity	4	15
GO:0097159	organic cyclic compound binding	2	16
GO:0097367	carbohydrate derivative binding	2	16
GO:0140098	catalytic activity, acting on RNA	3	16
GO:0140101	catalytic activity, acting on a tRNA	4	16
GO:0140640	catalytic activity, acting on a nucleic acid	2	16
GO:1901265	nucleoside phosphate binding	3	16
GO:1901363	heterocyclic compound binding	2	16

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	549	553	PF00656	0.490
CLV_NRD_NRD_1	413	415	PF00675	0.393
CLV_NRD_NRD_1	533	535	PF00675	0.284
CLV_NRD_NRD_1	644	646	PF00675	0.329
CLV_PCSK_KEX2_1	224	226	PF00082	0.416
CLV_PCSK_KEX2_1	533	535	PF00082	0.330
CLV_PCSK_PC1ET2_1	224	226	PF00082	0.416
CLV_PCSK_SKI1_1	119	123	PF00082	0.296
CLV_PCSK_SKI1_1	13	17	PF00082	0.498
CLV_PCSK_SKI1_1	370	374	PF00082	0.279
CLV_PCSK_SKI1_1	389	393	PF00082	0.182
CLV_PCSK_SKI1_1	52	56	PF00082	0.290
CLV_PCSK_SKI1_1	715	719	PF00082	0.310
DOC_CYCLIN_yCln2_LP_2	21	27	PF00134	0.382
DOC_CYCLIN_yCln2_LP_2	317	323	PF00134	0.318
DOC_MAPK_gen_1	337	347	PF00069	0.479
DOC_MAPK_HePTP_8	113	125	PF00069	0.394
DOC_MAPK_JIP1_4	119	125	PF00069	0.461
DOC_MAPK_MEF2A_6	116	125	PF00069	0.570
DOC_MAPK_MEF2A_6	340	349	PF00069	0.499
DOC_MAPK_MEF2A_6	483	491	PF00069	0.504
DOC_MAPK_MEF2A_6	566	574	PF00069	0.479
DOC_PP1_RVXF_1	164	171	PF00149	0.451
DOC_PP1_RVXF_1	84	91	PF00149	0.504
DOC_PP2B_LxvP_1	21	24	PF13499	0.504
DOC_PP2B_LxvP_1	406	409	PF13499	0.479
DOC_PP2B_LxvP_1	693	696	PF13499	0.479
DOC_PP4_FxxP_1	360	363	PF00568	0.479
DOC_PP4_FxxP_1	461	464	PF00568	0.479
DOC_PP4_FxxP_1	697	700	PF00568	0.479
DOC_USP7_MATH_1	580	584	PF00917	0.540
DOC_USP7_MATH_1	593	597	PF00917	0.580
DOC_USP7_MATH_1	668	672	PF00917	0.479
DOC_USP7_MATH_1	68	72	PF00917	0.546
DOC_USP7_UBL2_3	156	160	PF12436	0.463
DOC_USP7_UBL2_3	711	715	PF12436	0.535
DOC_USP7_UBL2_3	77	81	PF12436	0.436
DOC_WW_Pin1_4	105	110	PF00397	0.490
DOC_WW_Pin1_4	361	366	PF00397	0.490
DOC_WW_Pin1_4	552	557	PF00397	0.479
DOC_WW_Pin1_4	600	605	PF00397	0.553
DOC_WW_Pin1_4	69	74	PF00397	0.443
LIG_14-3-3_CanoR_1	119	124	PF00244	0.498
LIG_14-3-3_CanoR_1	324	333	PF00244	0.481
LIG_14-3-3_CanoR_1	412	421	PF00244	0.544
LIG_14-3-3_CanoR_1	503	513	PF00244	0.352
LIG_14-3-3_CanoR_1	594	598	PF00244	0.495
LIG_14-3-3_CanoR_1	630	640	PF00244	0.519
LIG_Actin_WH2_2	62	79	PF00022	0.522
LIG_APCC_ABBA_1	489	494	PF00400	0.400
LIG_BIR_II_1	1	5	PF00653	0.522
LIG_BIR_III_4	47	51	PF00653	0.394
LIG_deltaCOP1_diTrp_1	371	381	PF00928	0.490
LIG_deltaCOP1_diTrp_1	635	639	PF00928	0.352
LIG_FHA_1	144	150	PF00498	0.511
LIG_FHA_1	362	368	PF00498	0.509
LIG_FHA_1	425	431	PF00498	0.479
LIG_FHA_1	480	486	PF00498	0.479
LIG_FHA_1	49	55	PF00498	0.507
LIG_FHA_1	505	511	PF00498	0.456
LIG_FHA_1	626	632	PF00498	0.429
LIG_FHA_1	690	696	PF00498	0.522
LIG_FHA_1	716	722	PF00498	0.580
LIG_FHA_1	83	89	PF00498	0.535
LIG_FHA_2	148	154	PF00498	0.522
LIG_FHA_2	236	242	PF00498	0.344
LIG_FHA_2	31	37	PF00498	0.490
LIG_FHA_2	313	319	PF00498	0.484
LIG_FHA_2	414	420	PF00498	0.483
LIG_FHA_2	426	432	PF00498	0.485
LIG_FHA_2	518	524	PF00498	0.479
LIG_FHA_2	677	683	PF00498	0.511
LIG_FHA_2	70	76	PF00498	0.580
LIG_LIR_Apic_2	359	363	PF02991	0.479
LIG_LIR_Apic_2	458	464	PF02991	0.508
LIG_LIR_Gen_1	144	151	PF02991	0.606
LIG_LIR_Gen_1	230	240	PF02991	0.393
LIG_LIR_Gen_1	396	406	PF02991	0.490
LIG_LIR_Gen_1	634	643	PF02991	0.318
LIG_LIR_Gen_1	718	727	PF02991	0.547
LIG_LIR_Nem_3	108	113	PF02991	0.541
LIG_LIR_Nem_3	144	148	PF02991	0.606
LIG_LIR_Nem_3	150	154	PF02991	0.543
LIG_LIR_Nem_3	230	236	PF02991	0.387
LIG_LIR_Nem_3	244	248	PF02991	0.354
LIG_LIR_Nem_3	280	286	PF02991	0.396
LIG_LIR_Nem_3	329	335	PF02991	0.479
LIG_LIR_Nem_3	396	401	PF02991	0.490
LIG_LIR_Nem_3	634	639	PF02991	0.318
LIG_LIR_Nem_3	718	723	PF02991	0.547
LIG_Pex14_2	453	457	PF04695	0.479
LIG_SH2_CRK	145	149	PF00017	0.616
LIG_SH2_CRK	398	402	PF00017	0.479
LIG_SH2_NCK_1	398	402	PF00017	0.490
LIG_SH2_PTP2	233	236	PF00017	0.415
LIG_SH2_SRC	233	236	PF00017	0.433
LIG_SH2_STAP1	145	149	PF00017	0.469
LIG_SH2_STAP1	163	167	PF00017	0.371
LIG_SH2_STAP1	335	339	PF00017	0.479
LIG_SH2_STAP1	398	402	PF00017	0.481
LIG_SH2_STAP1	426	430	PF00017	0.479
LIG_SH2_STAT5	145	148	PF00017	0.461
LIG_SH2_STAT5	157	160	PF00017	0.368
LIG_SH2_STAT5	233	236	PF00017	0.392
LIG_SH2_STAT5	252	255	PF00017	0.375
LIG_SH2_STAT5	260	263	PF00017	0.362
LIG_SH2_STAT5	282	285	PF00017	0.378
LIG_SH2_STAT5	426	429	PF00017	0.497
LIG_SH3_3	327	333	PF00018	0.490
LIG_SH3_3	496	502	PF00018	0.378
LIG_SUMO_SIM_anti_2	35	42	PF11976	0.461
LIG_SUMO_SIM_anti_2	390	396	PF11976	0.546
LIG_SUMO_SIM_anti_2	506	513	PF11976	0.457
LIG_SUMO_SIM_anti_2	98	104	PF11976	0.558
LIG_SUMO_SIM_par_1	390	396	PF11976	0.546
LIG_TRAF2_1	150	153	PF00917	0.443
LIG_TRAF2_1	33	36	PF00917	0.520
LIG_TRAF2_1	520	523	PF00917	0.524
LIG_TRAF2_1	667	670	PF00917	0.483
LIG_TYR_ITIM	243	248	PF00017	0.387
LIG_WRC_WIRS_1	148	153	PF05994	0.443
LIG_WRC_WIRS_1	430	435	PF05994	0.540
LIG_WW_3	500	504	PF00397	0.377
MOD_CDK_SPxxK_3	361	368	PF00069	0.490
MOD_CDK_SPxxK_3	552	559	PF00069	0.479
MOD_CK1_1	211	217	PF00069	0.654
MOD_CK1_1	361	367	PF00069	0.490
MOD_CK1_1	71	77	PF00069	0.546
MOD_CK2_1	147	153	PF00069	0.533
MOD_CK2_1	235	241	PF00069	0.357
MOD_CK2_1	287	293	PF00069	0.483
MOD_CK2_1	30	36	PF00069	0.497
MOD_CK2_1	312	318	PF00069	0.480
MOD_CK2_1	397	403	PF00069	0.578
MOD_CK2_1	413	419	PF00069	0.428
MOD_CK2_1	425	431	PF00069	0.485
MOD_CK2_1	517	523	PF00069	0.479
MOD_CK2_1	664	670	PF00069	0.479
MOD_Cter_Amidation	412	415	PF01082	0.290
MOD_GlcNHglycan	130	134	PF01048	0.314
MOD_GlcNHglycan	173	176	PF01048	0.566
MOD_GlcNHglycan	210	213	PF01048	0.659
MOD_GlcNHglycan	229	232	PF01048	0.295
MOD_GlcNHglycan	247	251	PF01048	0.270
MOD_GlcNHglycan	590	593	PF01048	0.370
MOD_GlcNHglycan	666	669	PF01048	0.279
MOD_GSK3_1	125	132	PF00069	0.582
MOD_GSK3_1	143	150	PF00069	0.395
MOD_GSK3_1	425	432	PF00069	0.514
MOD_GSK3_1	474	481	PF00069	0.479
MOD_GSK3_1	514	521	PF00069	0.504
MOD_GSK3_1	64	71	PF00069	0.496
MOD_GSK3_1	664	671	PF00069	0.479
MOD_GSK3_1	711	718	PF00069	0.580
MOD_N-GLC_1	227	232	PF02516	0.462
MOD_N-GLC_1	552	557	PF02516	0.279
MOD_NEK2_1	129	134	PF00069	0.467
MOD_NEK2_1	285	290	PF00069	0.463
MOD_NEK2_1	528	533	PF00069	0.605
MOD_NEK2_1	55	60	PF00069	0.495
MOD_NEK2_2	397	402	PF00069	0.510
MOD_NEK2_2	50	55	PF00069	0.461
MOD_PIKK_1	504	510	PF00454	0.505
MOD_PKA_1	664	670	PF00069	0.479
MOD_PKA_2	323	329	PF00069	0.479
MOD_PKA_2	413	419	PF00069	0.580
MOD_PKA_2	588	594	PF00069	0.504
MOD_PKA_2	631	637	PF00069	0.531
MOD_Plk_1	125	131	PF00069	0.521
MOD_Plk_1	143	149	PF00069	0.483
MOD_Plk_1	227	233	PF00069	0.465
MOD_Plk_1	467	473	PF00069	0.522
MOD_Plk_1	580	586	PF00069	0.540
MOD_Plk_2-3	30	36	PF00069	0.504
MOD_Plk_4	326	332	PF00069	0.490
MOD_Plk_4	397	403	PF00069	0.510
MOD_Plk_4	429	435	PF00069	0.504
MOD_Plk_4	467	473	PF00069	0.492
MOD_Plk_4	50	56	PF00069	0.461
MOD_Plk_4	593	599	PF00069	0.505
MOD_Plk_4	716	722	PF00069	0.585
MOD_ProDKin_1	105	111	PF00069	0.490
MOD_ProDKin_1	361	367	PF00069	0.490
MOD_ProDKin_1	552	558	PF00069	0.479
MOD_ProDKin_1	600	606	PF00069	0.552
MOD_ProDKin_1	69	75	PF00069	0.443
MOD_SUMO_for_1	223	226	PF00179	0.403
MOD_SUMO_for_1	382	385	PF00179	0.580
MOD_SUMO_rev_2	211	219	PF00179	0.562
MOD_SUMO_rev_2	5	15	PF00179	0.333
MOD_SUMO_rev_2	666	674	PF00179	0.467
MOD_SUMO_rev_2	71	79	PF00179	0.546
TRG_DiLeu_BaEn_2	10	16	PF01217	0.267
TRG_DiLeu_BaEn_2	634	640	PF01217	0.459
TRG_DiLeu_BaEn_3	35	41	PF01217	0.394
TRG_DiLeu_BaLyEn_6	108	113	PF01217	0.412
TRG_ENDOCYTIC_2	145	148	PF00928	0.589
TRG_ENDOCYTIC_2	165	168	PF00928	0.353
TRG_ENDOCYTIC_2	233	236	PF00928	0.355
TRG_ENDOCYTIC_2	245	248	PF00928	0.403
TRG_ENDOCYTIC_2	282	285	PF00928	0.378
TRG_ENDOCYTIC_2	398	401	PF00928	0.479
TRG_ENDOCYTIC_2	402	405	PF00928	0.479
TRG_ER_diArg_1	367	370	PF00400	0.489
TRG_ER_diArg_1	411	414	PF00400	0.607
TRG_ER_diArg_1	533	535	PF00400	0.550
TRG_ER_diArg_1	630	633	PF00400	0.492
TRG_NES_CRM1_1	36	47	PF08389	0.394
TRG_Pf-PMV_PEXEL_1	533	538	PF00026	0.324
TRG_Pf-PMV_PEXEL_1	577	581	PF00026	0.372

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HY85	Leptomonas seymouri	84%	100%
A0A0S4JN58	Bodo saltans	57%	89%
A0A1X0P793	Trypanosomatidae	63%	90%
A0A3R7KPX0	Trypanosoma rangeli	64%	85%
A0A3S7WV15	Leishmania donovani	85%	100%
A0A3S7WV32	Leishmania donovani	94%	100%
A0M4Z3	Gramella forsetii (strain KT0803)	35%	100%
A1RSR6	Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3)	29%	100%
A3MV50	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	30%	100%
A4H9N1	Leishmania braziliensis	89%	99%
A4HXZ5	Leishmania infantum	94%	90%
A4HY00	Leishmania infantum	85%	100%
A4WJ22	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	28%	100%
A5FA31	Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101)	35%	100%
A6L5I2	Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC 11154)	37%	100%
A6LAP3	Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)	36%	100%
B0R3A3	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	39%	100%
B1Y963	Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta)	28%	100%
B2RJG1	Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)	38%	100%
B2UL92	Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc)	35%	100%
B3ER64	Amoebophilus asiaticus (strain 5a2)	37%	100%
B7J1X2	Borreliella burgdorferi (strain ZS7)	37%	100%
B9LUM0	Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)	41%	100%
D0A5B2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	90%
E9ARQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	86%	100%
O51363	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	37%	100%
O60155	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	44%	100%
P38708	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	47%	100%
Q0SNA5	Borreliella afzelii (strain PKo)	36%	100%
Q11Q45	Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)	34%	100%
Q18JG3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	39%	100%
Q1J0W2	Deinococcus geothermalis (strain DSM 11300 / AG-3a)	35%	100%
Q2ILX2	Anaeromyxobacter dehalogenans (strain 2CP-C)	39%	100%
Q2RZJ2	Salinibacter ruber (strain DSM 13855 / M31)	36%	100%
Q2W136	Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)	37%	100%
Q4QDS0	Leishmania major	84%	100%
Q4QDS1	Leishmania major	93%	100%
Q5LCF0	Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)	36%	100%
Q64TJ6	Bacteroides fragilis (strain YCH46)	36%	100%
Q661L6	Borrelia garinii subsp. bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi)	37%	100%
Q67PA6	Symbiobacterium thermophilum (strain T / IAM 14863)	41%	100%
Q6MIW2	Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100)	36%	100%
Q7MVS7	Porphyromonas gingivalis (strain ATCC BAA-308 / W83)	38%	100%
Q7X2P1	Sphingomonas elodea	36%	100%
Q81Z76	Bacillus anthracis	41%	100%
Q8A988	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	37%	100%
Q8I5R7	Plasmodium falciparum (isolate 3D7)	39%	97%
Q8SSD7	Encephalitozoon cuniculi (strain GB-M1)	48%	100%
Q8ZVQ9	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	28%	100%
Q9FYR6	Arabidopsis thaliana	37%	100%
Q9HS52	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	39%	100%
Q9M1R2	Arabidopsis thaliana	53%	100%
Q9RUW4	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	36%	100%
Q9Y9G0	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	32%	100%
V5AZ66	Trypanosoma cruzi	65%	90%