RING-type E3 ubiquitin transferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger), putative

Species:

Leishmania mexicana

UniProt:

E9ARQ2_LEIMU

TriTrypDb:

LmxM.18.1150

Length:

360

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 9

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 5

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ARQ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARQ2

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008152	metabolic process	1	1
GO:0016567	protein ubiquitination	7	1
GO:0019538	protein metabolic process	3	1
GO:0032446	protein modification by small protein conjugation	6	1
GO:0036211	protein modification process	4	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0044238	primary metabolic process	2	1
GO:0070647	protein modification by small protein conjugation or removal	5	1
GO:0071704	organic substance metabolic process	2	1
GO:1901564	organonitrogen compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0004842	ubiquitin-protein transferase activity	4	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0019787	ubiquitin-like protein transferase activity	3	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0061630	ubiquitin protein ligase activity	5	12
GO:0061659	ubiquitin-like protein ligase activity	4	12
GO:0140096	catalytic activity, acting on a protein	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	218	222	PF00656	0.668
CLV_NRD_NRD_1	173	175	PF00675	0.602
CLV_NRD_NRD_1	248	250	PF00675	0.451
CLV_PCSK_KEX2_1	215	217	PF00082	0.617
CLV_PCSK_KEX2_1	247	249	PF00082	0.482
CLV_PCSK_PC1ET2_1	215	217	PF00082	0.617
CLV_PCSK_PC1ET2_1	247	249	PF00082	0.500
CLV_PCSK_SKI1_1	174	178	PF00082	0.536
DEG_APCC_DBOX_1	156	164	PF00400	0.638
DOC_MAPK_gen_1	174	182	PF00069	0.581
DOC_MAPK_MEF2A_6	174	182	PF00069	0.502
DOC_MAPK_MEF2A_6	198	205	PF00069	0.366
DOC_MAPK_NFAT4_5	175	183	PF00069	0.583
DOC_PP2B_LxvP_1	346	349	PF13499	0.423
DOC_USP7_MATH_1	355	359	PF00917	0.713
DOC_USP7_MATH_1	88	92	PF00917	0.817
DOC_USP7_UBL2_3	175	179	PF12436	0.491
DOC_USP7_UBL2_3	190	194	PF12436	0.443
DOC_WW_Pin1_4	273	278	PF00397	0.580
LIG_14-3-3_CanoR_1	174	179	PF00244	0.505
LIG_14-3-3_CanoR_1	339	349	PF00244	0.316
LIG_FHA_1	118	124	PF00498	0.450
LIG_FHA_1	133	139	PF00498	0.399
LIG_FHA_1	175	181	PF00498	0.518
LIG_FHA_1	208	214	PF00498	0.523
LIG_FHA_2	21	27	PF00498	0.545
LIG_FHA_2	224	230	PF00498	0.643
LIG_FHA_2	236	242	PF00498	0.398
LIG_FHA_2	289	295	PF00498	0.708
LIG_MYND_1	158	162	PF01753	0.617
LIG_PDZ_Class_1	355	360	PF00595	0.681
LIG_SH2_GRB2like	41	44	PF00017	0.735
LIG_SH2_SRC	136	139	PF00017	0.413
LIG_SH2_STAT3	196	199	PF00017	0.429
LIG_SH2_STAT5	126	129	PF00017	0.561
LIG_SH2_STAT5	262	265	PF00017	0.446
LIG_SH3_1	330	336	PF00018	0.331
LIG_SH3_3	152	158	PF00018	0.453
LIG_SH3_3	330	336	PF00018	0.316
LIG_SH3_3	64	70	PF00018	0.763
LIG_TRAF2_1	184	187	PF00917	0.545
LIG_TRAF2_1	226	229	PF00917	0.571
LIG_WRC_WIRS_1	122	127	PF05994	0.467
MOD_CK1_1	112	118	PF00069	0.545
MOD_CK2_1	126	132	PF00069	0.443
MOD_CK2_1	181	187	PF00069	0.505
MOD_CK2_1	20	26	PF00069	0.757
MOD_CK2_1	223	229	PF00069	0.704
MOD_CK2_1	287	293	PF00069	0.732
MOD_GlcNHglycan	17	20	PF01048	0.685
MOD_GlcNHglycan	278	281	PF01048	0.620
MOD_GlcNHglycan	78	81	PF01048	0.782
MOD_GlcNHglycan	86	89	PF01048	0.545
MOD_GSK3_1	105	112	PF00069	0.479
MOD_GSK3_1	117	124	PF00069	0.445
MOD_GSK3_1	126	133	PF00069	0.481
MOD_GSK3_1	15	22	PF00069	0.601
MOD_GSK3_1	232	239	PF00069	0.468
MOD_GSK3_1	249	256	PF00069	0.382
MOD_GSK3_1	84	91	PF00069	0.692
MOD_N-GLC_1	147	152	PF02516	0.465
MOD_N-GLC_1	15	20	PF02516	0.808
MOD_N-GLC_1	223	228	PF02516	0.687
MOD_N-GLC_1	4	9	PF02516	0.783
MOD_N-GLC_1	42	47	PF02516	0.751
MOD_N-GLC_1	56	61	PF02516	0.792
MOD_N-GLC_1	76	81	PF02516	0.857
MOD_N-GLC_1	84	89	PF02516	0.616
MOD_NEK2_1	125	130	PF00069	0.405
MOD_NEK2_1	287	292	PF00069	0.620
MOD_NEK2_1	83	88	PF00069	0.552
MOD_NMyristoyl	1	7	PF02799	0.640
MOD_PIKK_1	117	123	PF00454	0.557
MOD_PIKK_1	249	255	PF00454	0.408
MOD_PIKK_1	4	10	PF00454	0.747
MOD_PKA_1	174	180	PF00069	0.585
MOD_Plk_1	109	115	PF00069	0.476
MOD_Plk_1	188	194	PF00069	0.602
MOD_Plk_1	235	241	PF00069	0.396
MOD_Plk_2-3	132	138	PF00069	0.516
MOD_Plk_2-3	181	187	PF00069	0.591
MOD_Plk_2-3	236	242	PF00069	0.459
MOD_Plk_4	112	118	PF00069	0.551
MOD_Plk_4	121	127	PF00069	0.511
MOD_Plk_4	341	347	PF00069	0.331
MOD_ProDKin_1	273	279	PF00069	0.581
MOD_SUMO_for_1	158	161	PF00179	0.516
MOD_SUMO_for_1	163	166	PF00179	0.497
TRG_ER_diArg_1	248	250	PF00400	0.427
TRG_NLS_MonoExtN_4	245	251	PF00514	0.476
TRG_Pf-PMV_PEXEL_1	249	253	PF00026	0.411

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMI8	Leptomonas seymouri	75%	98%
A0A0S4JNW6	Bodo saltans	34%	94%
A0A1X0P805	Trypanosomatidae	60%	97%
A0A3S7WV37	Leishmania donovani	95%	99%
A0A422P0Q0	Trypanosoma rangeli	55%	100%
A4H9M5	Leishmania braziliensis	79%	99%
A4HXY9	Leishmania infantum	95%	98%
D0A5B8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	47%	100%
Q4QDS7	Leishmania major	92%	100%
V5BNN3	Trypanosoma cruzi	54%	100%