Putative methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Multisite-specific tRNA:(cytosine-C(5))-methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9ARP8_LEIMU

TriTrypDb:

LmxM.18.1110

Length:

849

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 13
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005730	nucleolus	5	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ARP8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARP8

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	13
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009451	RNA modification	5	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0032259	methylation	2	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0043170	macromolecule metabolic process	3	13
GO:0043412	macromolecule modification	4	13
GO:0043414	macromolecule methylation	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0044260	obsolete cellular macromolecule metabolic process	3	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	14
GO:0003723	RNA binding	4	14
GO:0003824	catalytic activity	1	14
GO:0005488	binding	1	14
GO:0008168	methyltransferase activity	4	14
GO:0016740	transferase activity	2	14
GO:0016741	transferase activity, transferring one-carbon groups	3	14
GO:0097159	organic cyclic compound binding	2	14
GO:1901363	heterocyclic compound binding	2	14
GO:0000049	tRNA binding	5	1
GO:0008173	RNA methyltransferase activity	4	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016427	tRNA (cytosine) methyltransferase activity	6	1
GO:0016428	tRNA (cytosine-5-)-methyltransferase activity	7	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	194	198	PF00656	0.676
CLV_C14_Caspase3-7	472	476	PF00656	0.366
CLV_C14_Caspase3-7	64	68	PF00656	0.572
CLV_MEL_PAP_1	689	695	PF00089	0.484
CLV_NRD_NRD_1	133	135	PF00675	0.595
CLV_NRD_NRD_1	198	200	PF00675	0.737
CLV_NRD_NRD_1	3	5	PF00675	0.664
CLV_NRD_NRD_1	411	413	PF00675	0.256
CLV_NRD_NRD_1	539	541	PF00675	0.284
CLV_NRD_NRD_1	621	623	PF00675	0.434
CLV_NRD_NRD_1	687	689	PF00675	0.409
CLV_NRD_NRD_1	7	9	PF00675	0.615
CLV_NRD_NRD_1	99	101	PF00675	0.522
CLV_PCSK_FUR_1	357	361	PF00082	0.295
CLV_PCSK_KEX2_1	132	134	PF00082	0.601
CLV_PCSK_KEX2_1	2	4	PF00082	0.665
CLV_PCSK_KEX2_1	271	273	PF00082	0.506
CLV_PCSK_KEX2_1	359	361	PF00082	0.285
CLV_PCSK_KEX2_1	621	623	PF00082	0.425
CLV_PCSK_KEX2_1	687	689	PF00082	0.456
CLV_PCSK_KEX2_1	7	9	PF00082	0.594
CLV_PCSK_KEX2_1	99	101	PF00082	0.522
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.683
CLV_PCSK_PC1ET2_1	271	273	PF00082	0.531
CLV_PCSK_PC1ET2_1	359	361	PF00082	0.285
CLV_PCSK_PC7_1	3	9	PF00082	0.676
CLV_PCSK_PC7_1	683	689	PF00082	0.520
CLV_PCSK_SKI1_1	236	240	PF00082	0.520
CLV_PCSK_SKI1_1	337	341	PF00082	0.361
CLV_PCSK_SKI1_1	434	438	PF00082	0.303
CLV_PCSK_SKI1_1	540	544	PF00082	0.524
CLV_PCSK_SKI1_1	622	626	PF00082	0.391
CLV_PCSK_SKI1_1	697	701	PF00082	0.472
CLV_PCSK_SKI1_1	716	720	PF00082	0.569
DEG_Nend_UBRbox_1	1	4	PF02207	0.712
DEG_ODPH_VHL_1	680	693	PF01847	0.503
DEG_SIAH_1	731	739	PF03145	0.631
DEG_SPOP_SBC_1	598	602	PF00917	0.573
DOC_CKS1_1	213	218	PF01111	0.494
DOC_CKS1_1	238	243	PF01111	0.567
DOC_CKS1_1	625	630	PF01111	0.489
DOC_CYCLIN_RxL_1	434	446	PF00134	0.508
DOC_CYCLIN_RxL_1	683	694	PF00134	0.527
DOC_CYCLIN_yCln2_LP_2	507	513	PF00134	0.525
DOC_CYCLIN_yCln2_LP_2	565	571	PF00134	0.528
DOC_CYCLIN_yCln2_LP_2	625	631	PF00134	0.487
DOC_MAPK_gen_1	562	571	PF00069	0.564
DOC_MAPK_gen_1	638	647	PF00069	0.457
DOC_MAPK_gen_1	798	805	PF00069	0.332
DOC_MAPK_HePTP_8	635	647	PF00069	0.560
DOC_MAPK_MEF2A_6	638	647	PF00069	0.457
DOC_MAPK_MEF2A_6	798	805	PF00069	0.321
DOC_MAPK_NFAT4_5	798	806	PF00069	0.352
DOC_PP1_RVXF_1	685	692	PF00149	0.542
DOC_PP2B_LxvP_1	565	568	PF13499	0.493
DOC_PP4_FxxP_1	679	682	PF00568	0.541
DOC_USP7_MATH_1	137	141	PF00917	0.536
DOC_USP7_MATH_1	252	256	PF00917	0.528
DOC_USP7_MATH_1	598	602	PF00917	0.620
DOC_USP7_UBL2_3	345	349	PF12436	0.484
DOC_USP7_UBL2_3	733	737	PF12436	0.684
DOC_WW_Pin1_4	166	171	PF00397	0.649
DOC_WW_Pin1_4	212	217	PF00397	0.422
DOC_WW_Pin1_4	237	242	PF00397	0.550
DOC_WW_Pin1_4	419	424	PF00397	0.388
DOC_WW_Pin1_4	498	503	PF00397	0.481
DOC_WW_Pin1_4	624	629	PF00397	0.491
LIG_14-3-3_CanoR_1	100	108	PF00244	0.496
LIG_14-3-3_CanoR_1	132	139	PF00244	0.630
LIG_14-3-3_CanoR_1	236	241	PF00244	0.521
LIG_14-3-3_CanoR_1	278	288	PF00244	0.437
LIG_14-3-3_CanoR_1	347	355	PF00244	0.484
LIG_14-3-3_CanoR_1	415	425	PF00244	0.504
LIG_14-3-3_CanoR_1	622	628	PF00244	0.480
LIG_14-3-3_CanoR_1	683	691	PF00244	0.531
LIG_14-3-3_CanoR_1	825	829	PF00244	0.630
LIG_APCC_ABBA_1	524	529	PF00400	0.484
LIG_BRCT_BRCA1_1	675	679	PF00533	0.514
LIG_CSL_BTD_1	420	423	PF09270	0.421
LIG_deltaCOP1_diTrp_1	203	209	PF00928	0.458
LIG_deltaCOP1_diTrp_1	572	582	PF00928	0.413
LIG_Dynein_DLC8_1	347	353	PF01221	0.523
LIG_eIF4E_1	438	444	PF01652	0.366
LIG_FAT_LD_1	75	83	PF03623	0.546
LIG_FHA_1	144	150	PF00498	0.533
LIG_FHA_1	218	224	PF00498	0.392
LIG_FHA_1	233	239	PF00498	0.427
LIG_FHA_1	349	355	PF00498	0.484
LIG_FHA_1	444	450	PF00498	0.548
LIG_FHA_1	625	631	PF00498	0.444
LIG_FHA_1	96	102	PF00498	0.601
LIG_FHA_2	237	243	PF00498	0.560
LIG_FHA_2	305	311	PF00498	0.540
LIG_FHA_2	367	373	PF00498	0.484
LIG_FHA_2	470	476	PF00498	0.463
LIG_FHA_2	752	758	PF00498	0.613
LIG_LIR_Apic_2	546	550	PF02991	0.507
LIG_LIR_Apic_2	637	643	PF02991	0.465
LIG_LIR_Apic_2	676	682	PF02991	0.488
LIG_LIR_Gen_1	156	162	PF02991	0.484
LIG_LIR_Gen_1	287	296	PF02991	0.439
LIG_LIR_Gen_1	39	48	PF02991	0.587
LIG_LIR_Gen_1	393	404	PF02991	0.467
LIG_LIR_Gen_1	573	583	PF02991	0.438
LIG_LIR_Gen_1	89	97	PF02991	0.594
LIG_LIR_Nem_3	156	161	PF02991	0.463
LIG_LIR_Nem_3	203	209	PF02991	0.481
LIG_LIR_Nem_3	287	291	PF02991	0.448
LIG_LIR_Nem_3	39	43	PF02991	0.533
LIG_LIR_Nem_3	393	399	PF02991	0.467
LIG_LIR_Nem_3	573	579	PF02991	0.406
LIG_LIR_Nem_3	580	585	PF02991	0.401
LIG_LIR_Nem_3	590	596	PF02991	0.426
LIG_LIR_Nem_3	713	718	PF02991	0.583
LIG_LIR_Nem_3	89	95	PF02991	0.591
LIG_MYND_1	510	514	PF01753	0.574
LIG_NRBOX	699	705	PF00104	0.565
LIG_NRBOX	74	80	PF00104	0.544
LIG_NRBOX	741	747	PF00104	0.619
LIG_NRBOX	759	765	PF00104	0.453
LIG_Pex14_1	376	380	PF04695	0.508
LIG_Pex14_1	421	425	PF04695	0.484
LIG_Pex14_2	535	539	PF04695	0.479
LIG_SH2_CRK	361	365	PF00017	0.508
LIG_SH2_CRK	640	644	PF00017	0.504
LIG_SH2_NCK_1	596	600	PF00017	0.428
LIG_SH2_STAT5	275	278	PF00017	0.472
LIG_SH2_STAT5	35	38	PF00017	0.500
LIG_SH2_STAT5	380	383	PF00017	0.545
LIG_SH2_STAT5	425	428	PF00017	0.479
LIG_SH2_STAT5	710	713	PF00017	0.703
LIG_SH2_STAT5	791	794	PF00017	0.391
LIG_SH3_3	164	170	PF00018	0.645
LIG_SH3_3	243	249	PF00018	0.471
LIG_SH3_3	371	377	PF00018	0.505
LIG_SH3_3	381	387	PF00018	0.512
LIG_SH3_3	507	513	PF00018	0.538
LIG_SH3_3	539	545	PF00018	0.541
LIG_SUMO_SIM_anti_2	475	481	PF11976	0.484
LIG_SUMO_SIM_anti_2	756	764	PF11976	0.581
LIG_TRAF2_1	287	290	PF00917	0.370
LIG_TRAF2_1	756	759	PF00917	0.622
LIG_TRAF2_1	841	844	PF00917	0.799
LIG_TRFH_1	611	615	PF08558	0.462
LIG_TYR_ITIM	436	441	PF00017	0.177
LIG_WRC_WIRS_1	470	475	PF05994	0.313
LIG_WRC_WIRS_1	579	584	PF05994	0.507
LIG_WW_1	513	516	PF00397	0.468
MOD_CDK_SPxxK_3	166	173	PF00069	0.681
MOD_CK1_1	104	110	PF00069	0.469
MOD_CK1_1	121	127	PF00069	0.553
MOD_CK1_1	212	218	PF00069	0.467
MOD_CK1_1	284	290	PF00069	0.398
MOD_CK1_1	416	422	PF00069	0.379
MOD_CK1_1	453	459	PF00069	0.318
MOD_CK1_1	600	606	PF00069	0.589
MOD_CK1_1	750	756	PF00069	0.644
MOD_CK2_1	184	190	PF00069	0.743
MOD_CK2_1	284	290	PF00069	0.374
MOD_CK2_1	70	76	PF00069	0.560
MOD_CK2_1	737	743	PF00069	0.666
MOD_CK2_1	753	759	PF00069	0.481
MOD_CK2_1	763	769	PF00069	0.460
MOD_GlcNHglycan	123	126	PF01048	0.662
MOD_GlcNHglycan	139	142	PF01048	0.507
MOD_GlcNHglycan	256	259	PF01048	0.527
MOD_GlcNHglycan	27	30	PF01048	0.403
MOD_GlcNHglycan	283	286	PF01048	0.431
MOD_GlcNHglycan	292	295	PF01048	0.375
MOD_GlcNHglycan	327	330	PF01048	0.418
MOD_GlcNHglycan	331	334	PF01048	0.388
MOD_GlcNHglycan	405	408	PF01048	0.318
MOD_GlcNHglycan	494	497	PF01048	0.399
MOD_GlcNHglycan	54	57	PF01048	0.690
MOD_GlcNHglycan	602	605	PF01048	0.570
MOD_GlcNHglycan	675	678	PF01048	0.382
MOD_GlcNHglycan	72	75	PF01048	0.395
MOD_GlcNHglycan	792	795	PF01048	0.416
MOD_GlcNHglycan	834	837	PF01048	0.742
MOD_GSK3_1	232	239	PF00069	0.512
MOD_GSK3_1	254	261	PF00069	0.537
MOD_GSK3_1	262	269	PF00069	0.520
MOD_GSK3_1	325	332	PF00069	0.414
MOD_GSK3_1	382	389	PF00069	0.428
MOD_GSK3_1	403	410	PF00069	0.318
MOD_GSK3_1	413	420	PF00069	0.318
MOD_GSK3_1	421	428	PF00069	0.318
MOD_GSK3_1	469	476	PF00069	0.177
MOD_GSK3_1	747	754	PF00069	0.617
MOD_GSK3_1	776	783	PF00069	0.518
MOD_GSK3_1	790	797	PF00069	0.392
MOD_LATS_1	130	136	PF00433	0.727
MOD_N-GLC_1	184	189	PF02516	0.700
MOD_N-GLC_1	195	200	PF02516	0.540
MOD_N-GLC_1	577	582	PF02516	0.432
MOD_N-GLC_1	830	835	PF02516	0.735
MOD_NEK2_1	118	123	PF00069	0.592
MOD_NEK2_1	143	148	PF00069	0.570
MOD_NEK2_1	209	214	PF00069	0.491
MOD_NEK2_1	443	448	PF00069	0.385
MOD_NEK2_1	450	455	PF00069	0.306
MOD_NEK2_1	691	696	PF00069	0.549
MOD_NEK2_1	763	768	PF00069	0.516
MOD_NEK2_2	407	412	PF00069	0.318
MOD_PIKK_1	101	107	PF00454	0.521
MOD_PIKK_1	118	124	PF00454	0.525
MOD_PIKK_1	348	354	PF00454	0.343
MOD_PIKK_1	41	47	PF00454	0.635
MOD_PIKK_1	429	435	PF00454	0.343
MOD_PIKK_1	744	750	PF00454	0.645
MOD_PIKK_1	763	769	PF00454	0.560
MOD_PK_1	413	419	PF00069	0.421
MOD_PK_1	77	83	PF00069	0.535
MOD_PKA_1	132	138	PF00069	0.726
MOD_PKA_2	132	138	PF00069	0.705
MOD_PKA_2	153	159	PF00069	0.575
MOD_PKA_2	682	688	PF00069	0.516
MOD_PKA_2	691	697	PF00069	0.475
MOD_PKA_2	824	830	PF00069	0.724
MOD_PKA_2	95	101	PF00069	0.581
MOD_Plk_1	143	149	PF00069	0.570
MOD_Plk_1	413	419	PF00069	0.421
MOD_Plk_1	577	583	PF00069	0.447
MOD_Plk_1	589	595	PF00069	0.490
MOD_Plk_1	751	757	PF00069	0.574
MOD_Plk_4	304	310	PF00069	0.346
MOD_Plk_4	322	328	PF00069	0.376
MOD_Plk_4	421	427	PF00069	0.358
MOD_Plk_4	548	554	PF00069	0.583
MOD_Plk_4	578	584	PF00069	0.473
MOD_Plk_4	589	595	PF00069	0.505
MOD_Plk_4	675	681	PF00069	0.413
MOD_Plk_4	77	83	PF00069	0.527
MOD_Plk_4	824	830	PF00069	0.684
MOD_ProDKin_1	166	172	PF00069	0.644
MOD_ProDKin_1	212	218	PF00069	0.422
MOD_ProDKin_1	237	243	PF00069	0.542
MOD_ProDKin_1	419	425	PF00069	0.208
MOD_ProDKin_1	498	504	PF00069	0.339
MOD_ProDKin_1	624	630	PF00069	0.490
MOD_SUMO_rev_2	656	663	PF00179	0.466
TRG_DiLeu_BaEn_1	759	764	PF01217	0.605
TRG_DiLeu_BaEn_1	824	829	PF01217	0.549
TRG_DiLeu_BaEn_2	204	210	PF01217	0.489
TRG_DiLeu_BaEn_2	674	680	PF01217	0.510
TRG_DiLeu_LyEn_5	145	150	PF01217	0.470
TRG_ENDOCYTIC_2	438	441	PF00928	0.177
TRG_ER_diArg_1	132	134	PF00400	0.614
TRG_ER_diArg_1	620	622	PF00400	0.463
TRG_ER_diArg_1	635	638	PF00400	0.549
TRG_ER_diArg_1	687	689	PF00400	0.461
TRG_ER_diArg_1	7	10	PF00400	0.626
TRG_ER_diArg_1	99	101	PF00400	0.541
TRG_Pf-PMV_PEXEL_1	398	402	PF00026	0.376
TRG_Pf-PMV_PEXEL_1	697	701	PF00026	0.549

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8H1	Leptomonas seymouri	71%	99%
A0A0S4KJ12	Bodo saltans	42%	100%
A0A1X0P6Y3	Trypanosomatidae	53%	100%
A0A3R7M922	Trypanosoma rangeli	52%	100%
A0A3R7NNX1	Trypanosoma rangeli	25%	100%
A0A3S7WV22	Leishmania donovani	92%	100%
A4H9M1	Leishmania braziliensis	84%	99%
A4HXY5	Leishmania infantum	93%	100%
D0A386	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
D0A5C2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
Q4QDT1	Leishmania major	91%	100%
V5DFH6	Trypanosoma cruzi	52%	100%