Putative DNA-directed RNA polymerase II

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase I subunit RPB5z, putative

Species:

Leishmania mexicana

UniProt:

E9ARL6_LEIMU

TriTrypDb:

LmxM.18.0790

Length:

342

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	15
GO:0030880	RNA polymerase complex	3	15
GO:0032991	protein-containing complex	1	15
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	13
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	15
GO:0140513	nuclear protein-containing complex	2	13
GO:0140535	intracellular protein-containing complex	2	15
GO:1902494	catalytic complex	2	15
GO:1990234	transferase complex	3	15
GO:0005634	nucleus	5	2
GO:0005665	RNA polymerase II, core complex	4	2
GO:0005666	RNA polymerase III complex	4	2
GO:0005736	RNA polymerase I complex	4	2
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9ARL6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARL6

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	15
GO:0006351	DNA-templated transcription	7	15
GO:0006725	cellular aromatic compound metabolic process	3	15
GO:0006807	nitrogen compound metabolic process	2	15
GO:0008152	metabolic process	1	15
GO:0009058	biosynthetic process	2	15
GO:0009059	macromolecule biosynthetic process	4	15
GO:0009987	cellular process	1	15
GO:0016070	RNA metabolic process	5	15
GO:0018130	heterocycle biosynthetic process	4	15
GO:0019438	aromatic compound biosynthetic process	4	15
GO:0032774	RNA biosynthetic process	5	15
GO:0034641	cellular nitrogen compound metabolic process	3	15
GO:0034654	nucleobase-containing compound biosynthetic process	4	15
GO:0043170	macromolecule metabolic process	3	15
GO:0044237	cellular metabolic process	2	15
GO:0044238	primary metabolic process	2	15
GO:0044249	cellular biosynthetic process	3	15
GO:0044271	cellular nitrogen compound biosynthetic process	4	15
GO:0046483	heterocycle metabolic process	3	15
GO:0071704	organic substance metabolic process	2	15
GO:0090304	nucleic acid metabolic process	4	15
GO:0097659	nucleic acid-templated transcription	6	15
GO:1901360	organic cyclic compound metabolic process	3	15
GO:1901362	organic cyclic compound biosynthetic process	4	15
GO:1901576	organic substance biosynthetic process	3	15

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	15
GO:0003677	DNA binding	4	15
GO:0003824	catalytic activity	1	15
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	15
GO:0005488	binding	1	15
GO:0016740	transferase activity	2	15
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	15
GO:0016779	nucleotidyltransferase activity	4	15
GO:0034062	5'-3' RNA polymerase activity	5	15
GO:0097159	organic cyclic compound binding	2	15
GO:0097747	RNA polymerase activity	4	15
GO:0140098	catalytic activity, acting on RNA	3	15
GO:0140640	catalytic activity, acting on a nucleic acid	2	15
GO:1901363	heterocyclic compound binding	2	15

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	181	185	PF00656	0.605
CLV_C14_Caspase3-7	95	99	PF00656	0.564
CLV_NRD_NRD_1	116	118	PF00675	0.544
CLV_NRD_NRD_1	139	141	PF00675	0.732
CLV_NRD_NRD_1	294	296	PF00675	0.350
CLV_NRD_NRD_1	87	89	PF00675	0.620
CLV_PCSK_KEX2_1	116	118	PF00082	0.525
CLV_PCSK_KEX2_1	139	141	PF00082	0.732
CLV_PCSK_KEX2_1	87	89	PF00082	0.620
CLV_PCSK_SKI1_1	139	143	PF00082	0.681
CLV_PCSK_SKI1_1	190	194	PF00082	0.558
CLV_PCSK_SKI1_1	217	221	PF00082	0.493
CLV_PCSK_SKI1_1	88	92	PF00082	0.675
CLV_Separin_Metazoa	113	117	PF03568	0.523
DOC_MAPK_gen_1	190	200	PF00069	0.448
DOC_MAPK_gen_1	220	229	PF00069	0.455
DOC_MAPK_MEF2A_6	193	200	PF00069	0.394
DOC_MAPK_MEF2A_6	232	239	PF00069	0.581
DOC_MAPK_RevD_3	281	296	PF00069	0.485
DOC_USP7_MATH_1	68	72	PF00917	0.615
DOC_USP7_UBL2_3	164	168	PF12436	0.622
DOC_USP7_UBL2_3	186	190	PF12436	0.604
DOC_USP7_UBL2_3	205	209	PF12436	0.374
DOC_USP7_UBL2_3	217	221	PF12436	0.359
DOC_WW_Pin1_4	153	158	PF00397	0.701
DOC_WW_Pin1_4	200	205	PF00397	0.455
DOC_WW_Pin1_4	281	286	PF00397	0.508
DOC_WW_Pin1_4	61	66	PF00397	0.478
DOC_WW_Pin1_4	82	87	PF00397	0.785
LIG_14-3-3_CanoR_1	131	137	PF00244	0.494
LIG_14-3-3_CanoR_1	139	144	PF00244	0.666
LIG_14-3-3_CanoR_1	14	20	PF00244	0.377
LIG_APCC_ABBA_1	259	264	PF00400	0.395
LIG_BIR_II_1	1	5	PF00653	0.532
LIG_deltaCOP1_diTrp_1	53	61	PF00928	0.407
LIG_FHA_1	131	137	PF00498	0.430
LIG_FHA_2	250	256	PF00498	0.516
LIG_FHA_2	282	288	PF00498	0.540
LIG_LIR_Gen_1	122	130	PF02991	0.483
LIG_LIR_Nem_3	332	338	PF02991	0.491
LIG_Pex14_2	57	61	PF04695	0.402
LIG_SH2_GRB2like	46	49	PF00017	0.328
LIG_SH2_NCK_1	176	180	PF00017	0.529
LIG_SH2_STAP1	105	109	PF00017	0.604
LIG_SH2_STAP1	176	180	PF00017	0.529
LIG_SH2_STAT5	28	31	PF00017	0.402
LIG_SH2_STAT5	314	317	PF00017	0.456
LIG_SH3_3	37	43	PF00018	0.469
LIG_SH3_3	59	65	PF00018	0.475
LIG_TRAF2_1	3	6	PF00917	0.448
LIG_TYR_ITIM	312	317	PF00017	0.456
LIG_WRC_WIRS_1	211	216	PF05994	0.390
MOD_CDC14_SPxK_1	64	67	PF00782	0.433
MOD_CDC14_SPxK_1	85	88	PF00782	0.527
MOD_CDK_SPK_2	200	205	PF00069	0.417
MOD_CDK_SPK_2	82	87	PF00069	0.525
MOD_CDK_SPxK_1	61	67	PF00069	0.515
MOD_CDK_SPxK_1	82	88	PF00069	0.520
MOD_CK1_1	146	152	PF00069	0.708
MOD_CK1_1	159	165	PF00069	0.629
MOD_CK1_1	71	77	PF00069	0.636
MOD_CK1_1	82	88	PF00069	0.818
MOD_CK2_1	204	210	PF00069	0.410
MOD_CK2_1	249	255	PF00069	0.507
MOD_CK2_1	281	287	PF00069	0.483
MOD_GlcNHglycan	158	161	PF01048	0.808
MOD_GlcNHglycan	180	183	PF01048	0.539
MOD_GlcNHglycan	200	203	PF01048	0.356
MOD_GlcNHglycan	243	247	PF01048	0.498
MOD_GlcNHglycan	33	36	PF01048	0.432
MOD_GlcNHglycan	331	334	PF01048	0.296
MOD_GSK3_1	139	146	PF00069	0.638
MOD_GSK3_1	155	162	PF00069	0.594
MOD_GSK3_1	200	207	PF00069	0.424
MOD_GSK3_1	329	336	PF00069	0.517
MOD_N-GLC_1	153	158	PF02516	0.511
MOD_NEK2_1	130	135	PF00069	0.462
MOD_NEK2_1	143	148	PF00069	0.691
MOD_NEK2_2	204	209	PF00069	0.524
MOD_PKA_1	139	145	PF00069	0.546
MOD_PKA_1	221	227	PF00069	0.470
MOD_PKA_2	130	136	PF00069	0.510
MOD_PKA_2	139	145	PF00069	0.676
MOD_PKA_2	318	324	PF00069	0.476
MOD_PKA_2	69	75	PF00069	0.585
MOD_Plk_2-3	210	216	PF00069	0.389
MOD_Plk_4	143	149	PF00069	0.788
MOD_Plk_4	15	21	PF00069	0.374
MOD_Plk_4	222	228	PF00069	0.446
MOD_Plk_4	318	324	PF00069	0.456
MOD_Plk_4	333	339	PF00069	0.456
MOD_ProDKin_1	153	159	PF00069	0.702
MOD_ProDKin_1	200	206	PF00069	0.449
MOD_ProDKin_1	281	287	PF00069	0.508
MOD_ProDKin_1	61	67	PF00069	0.487
MOD_ProDKin_1	82	88	PF00069	0.786
MOD_SUMO_for_1	163	166	PF00179	0.757
MOD_SUMO_for_1	90	93	PF00179	0.804
MOD_SUMO_rev_2	181	187	PF00179	0.446
MOD_SUMO_rev_2	2	10	PF00179	0.431
MOD_SUMO_rev_2	289	297	PF00179	0.562
TRG_DiLeu_BaEn_2	286	292	PF01217	0.512
TRG_ENDOCYTIC_2	314	317	PF00928	0.485
TRG_ENDOCYTIC_2	338	341	PF00928	0.485
TRG_ER_diArg_1	115	117	PF00400	0.583
TRG_ER_diArg_1	138	140	PF00400	0.562
TRG_ER_diArg_1	86	88	PF00400	0.620
TRG_NLS_MonoExtN_4	217	224	PF00514	0.497

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8I4	Leptomonas seymouri	68%	100%
A0A1X0P763	Trypanosomatidae	44%	100%
A0A3Q8ID21	Leishmania donovani	33%	100%
A0A3R7KXJ2	Trypanosoma rangeli	44%	100%
A0A3S5H734	Leishmania donovani	93%	100%
A4H9J0	Leishmania braziliensis	79%	94%
A4HXV2	Leishmania infantum	33%	100%
A4HXV3	Leishmania infantum	93%	100%
C9ZZV0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9ARL5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
Q4QDW3	Leishmania major	94%	100%
Q4QDW4	Leishmania major	33%	100%
V5B6J4	Trypanosoma cruzi	43%	100%