Aconitate hydratase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Aconitate hydratase

Gene product:

aconitase, putative

Species:

Leishmania mexicana

UniProt:

E9ARI8_LEIMU

TriTrypDb:

LmxM.18.0510

Length:

896

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 12

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 6

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ARI8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARI8

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	1
GO:0006099	tricarboxylic acid cycle	3	1
GO:0006101	citrate metabolic process	7	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0019752	carboxylic acid metabolic process	5	1
GO:0043436	oxoacid metabolic process	4	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044281	small molecule metabolic process	2	1
GO:0071704	organic substance metabolic process	2	1
GO:0072350	tricarboxylic acid metabolic process	6	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0003994	aconitate hydratase activity	5	12
GO:0005488	binding	1	12
GO:0016829	lyase activity	2	12
GO:0016835	carbon-oxygen lyase activity	3	12
GO:0016836	hydro-lyase activity	4	12
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0047780	citrate dehydratase activity	5	12
GO:0051536	iron-sulfur cluster binding	3	12
GO:0051539	4 iron, 4 sulfur cluster binding	4	12
GO:0051540	metal cluster binding	2	12
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0003729	mRNA binding	5	1
GO:0030350	iron-responsive element binding	6	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	125	127	PF00675	0.188
CLV_NRD_NRD_1	281	283	PF00675	0.264
CLV_NRD_NRD_1	708	710	PF00675	0.264
CLV_NRD_NRD_1	717	719	PF00675	0.264
CLV_NRD_NRD_1	83	85	PF00675	0.188
CLV_PCSK_KEX2_1	125	127	PF00082	0.311
CLV_PCSK_KEX2_1	384	386	PF00082	0.264
CLV_PCSK_KEX2_1	708	710	PF00082	0.262
CLV_PCSK_KEX2_1	717	719	PF00082	0.266
CLV_PCSK_PC1ET2_1	384	386	PF00082	0.264
CLV_PCSK_PC7_1	704	710	PF00082	0.188
CLV_PCSK_SKI1_1	397	401	PF00082	0.303
CLV_PCSK_SKI1_1	421	425	PF00082	0.351
CLV_PCSK_SKI1_1	529	533	PF00082	0.331
CLV_PCSK_SKI1_1	53	57	PF00082	0.350
CLV_PCSK_SKI1_1	607	611	PF00082	0.423
CLV_PCSK_SKI1_1	864	868	PF00082	0.470
CLV_PCSK_SKI1_1	95	99	PF00082	0.264
DEG_APCC_DBOX_1	305	313	PF00400	0.475
DEG_APCC_DBOX_1	811	819	PF00400	0.464
DEG_Nend_Nbox_1	1	3	PF02207	0.558
DOC_ANK_TNKS_1	736	743	PF00023	0.464
DOC_CKS1_1	608	613	PF01111	0.420
DOC_MAPK_gen_1	125	133	PF00069	0.388
DOC_MAPK_gen_1	384	392	PF00069	0.471
DOC_MAPK_gen_1	717	725	PF00069	0.464
DOC_MAPK_gen_1	84	91	PF00069	0.388
DOC_MAPK_gen_1	869	878	PF00069	0.383
DOC_MAPK_MEF2A_6	125	133	PF00069	0.476
DOC_MAPK_MEF2A_6	84	91	PF00069	0.388
DOC_PP1_RVXF_1	287	294	PF00149	0.464
DOC_PP2B_LxvP_1	181	184	PF13499	0.464
DOC_PP2B_LxvP_1	471	474	PF13499	0.459
DOC_PP2B_LxvP_1	659	662	PF13499	0.509
DOC_PP4_FxxP_1	293	296	PF00568	0.475
DOC_PP4_FxxP_1	412	415	PF00568	0.464
DOC_USP7_MATH_1	401	405	PF00917	0.565
DOC_USP7_MATH_1	443	447	PF00917	0.495
DOC_USP7_MATH_1	525	529	PF00917	0.484
DOC_USP7_UBL2_3	259	263	PF12436	0.531
DOC_USP7_UBL2_3	279	283	PF12436	0.370
DOC_USP7_UBL2_3	406	410	PF12436	0.465
DOC_USP7_UBL2_3	465	469	PF12436	0.464
DOC_WW_Pin1_4	11	16	PF00397	0.469
DOC_WW_Pin1_4	479	484	PF00397	0.464
DOC_WW_Pin1_4	560	565	PF00397	0.464
DOC_WW_Pin1_4	607	612	PF00397	0.386
DOC_WW_Pin1_4	647	652	PF00397	0.423
DOC_WW_Pin1_4	683	688	PF00397	0.377
DOC_WW_Pin1_4	692	697	PF00397	0.386
LIG_14-3-3_CanoR_1	306	310	PF00244	0.475
LIG_APCC_ABBA_1	673	678	PF00400	0.348
LIG_BRCT_BRCA1_1	173	177	PF00533	0.464
LIG_BRCT_BRCA1_1	395	399	PF00533	0.566
LIG_BRCT_BRCA1_1	842	846	PF00533	0.372
LIG_CtBP_PxDLS_1	132	136	PF00389	0.464
LIG_deltaCOP1_diTrp_1	788	795	PF00928	0.489
LIG_DLG_GKlike_1	345	353	PF00625	0.486
LIG_EH_1	15	19	PF12763	0.382
LIG_EH1_1	49	57	PF00400	0.316
LIG_eIF4E_1	608	614	PF01652	0.367
LIG_FHA_1	219	225	PF00498	0.452
LIG_FHA_1	259	265	PF00498	0.464
LIG_FHA_1	269	275	PF00498	0.464
LIG_FHA_1	364	370	PF00498	0.499
LIG_FHA_1	452	458	PF00498	0.522
LIG_FHA_1	487	493	PF00498	0.464
LIG_FHA_1	608	614	PF00498	0.380
LIG_FHA_1	680	686	PF00498	0.409
LIG_FHA_1	771	777	PF00498	0.494
LIG_FHA_1	865	871	PF00498	0.483
LIG_FHA_2	267	273	PF00498	0.464
LIG_FHA_2	356	362	PF00498	0.477
LIG_FHA_2	835	841	PF00498	0.386
LIG_FHA_2	872	878	PF00498	0.381
LIG_LIR_Apic_2	292	296	PF02991	0.475
LIG_LIR_Gen_1	174	185	PF02991	0.464
LIG_LIR_Gen_1	42	52	PF02991	0.361
LIG_LIR_Gen_1	616	624	PF02991	0.415
LIG_LIR_Gen_1	805	815	PF02991	0.464
LIG_LIR_LC3C_4	134	137	PF02991	0.489
LIG_LIR_LC3C_4	563	568	PF02991	0.449
LIG_LIR_Nem_3	100	104	PF02991	0.464
LIG_LIR_Nem_3	165	170	PF02991	0.464
LIG_LIR_Nem_3	174	180	PF02991	0.464
LIG_LIR_Nem_3	319	325	PF02991	0.464
LIG_LIR_Nem_3	32	38	PF02991	0.370
LIG_LIR_Nem_3	407	412	PF02991	0.475
LIG_LIR_Nem_3	42	48	PF02991	0.338
LIG_LIR_Nem_3	612	617	PF02991	0.364
LIG_LIR_Nem_3	710	714	PF02991	0.464
LIG_LIR_Nem_3	744	750	PF02991	0.531
LIG_LIR_Nem_3	805	810	PF02991	0.464
LIG_PCNA_yPIPBox_3	592	605	PF02747	0.382
LIG_PCNA_yPIPBox_3	879	890	PF02747	0.382
LIG_Pex14_2	18	22	PF04695	0.534
LIG_Pex14_2	842	846	PF04695	0.372
LIG_PTB_Apo_2	44	51	PF02174	0.370
LIG_PTB_Apo_2	584	591	PF02174	0.381
LIG_PTB_Apo_2	632	639	PF02174	0.577
LIG_PTB_Phospho_1	584	590	PF10480	0.430
LIG_RPA_C_Fungi	340	352	PF08784	0.195
LIG_SH2_CRK	45	49	PF00017	0.466
LIG_SH2_CRK	618	622	PF00017	0.399
LIG_SH2_GRB2like	618	621	PF00017	0.495
LIG_SH2_NCK_1	618	622	PF00017	0.418
LIG_SH2_PTP2	608	611	PF00017	0.394
LIG_SH2_SRC	618	621	PF00017	0.409
LIG_SH2_SRC	647	650	PF00017	0.423
LIG_SH2_STAP1	365	369	PF00017	0.368
LIG_SH2_STAT5	197	200	PF00017	0.302
LIG_SH2_STAT5	322	325	PF00017	0.337
LIG_SH2_STAT5	332	335	PF00017	0.263
LIG_SH2_STAT5	349	352	PF00017	0.298
LIG_SH2_STAT5	365	368	PF00017	0.290
LIG_SH2_STAT5	425	428	PF00017	0.362
LIG_SH2_STAT5	491	494	PF00017	0.302
LIG_SH2_STAT5	560	563	PF00017	0.302
LIG_SH2_STAT5	590	593	PF00017	0.423
LIG_SH2_STAT5	608	611	PF00017	0.260
LIG_SH2_STAT5	647	650	PF00017	0.371
LIG_SH2_STAT5	653	656	PF00017	0.410
LIG_SH2_STAT5	747	750	PF00017	0.356
LIG_SH2_STAT5	880	883	PF00017	0.368
LIG_SH2_STAT5	889	892	PF00017	0.308
LIG_SH3_1	126	132	PF00018	0.295
LIG_SH3_2	90	95	PF14604	0.302
LIG_SH3_3	126	132	PF00018	0.302
LIG_SH3_3	377	383	PF00018	0.309
LIG_SH3_3	87	93	PF00018	0.302
LIG_SUMO_SIM_anti_2	178	184	PF11976	0.317
LIG_SUMO_SIM_anti_2	600	606	PF11976	0.394
LIG_TRAF2_2	822	827	PF00917	0.270
LIG_TYR_ITSM	318	325	PF00017	0.444
MOD_CDK_SPxxK_3	692	699	PF00069	0.400
MOD_CK1_1	219	225	PF00069	0.302
MOD_CK1_1	404	410	PF00069	0.467
MOD_CK1_1	451	457	PF00069	0.473
MOD_CK1_1	482	488	PF00069	0.314
MOD_CK1_1	679	685	PF00069	0.390
MOD_CK1_1	783	789	PF00069	0.335
MOD_CK2_1	355	361	PF00069	0.302
MOD_CK2_1	594	600	PF00069	0.387
MOD_CK2_1	834	840	PF00069	0.385
MOD_CK2_1	871	877	PF00069	0.386
MOD_GlcNHglycan	186	189	PF01048	0.302
MOD_GlcNHglycan	31	34	PF01048	0.529
MOD_GlcNHglycan	338	341	PF01048	0.427
MOD_GlcNHglycan	403	406	PF01048	0.393
MOD_GlcNHglycan	519	522	PF01048	0.317
MOD_GlcNHglycan	542	545	PF01048	0.304
MOD_GlcNHglycan	57	60	PF01048	0.231
MOD_GlcNHglycan	782	785	PF01048	0.337
MOD_GlcNHglycan	846	849	PF01048	0.424
MOD_GSK3_1	210	217	PF00069	0.302
MOD_GSK3_1	443	450	PF00069	0.337
MOD_GSK3_1	482	489	PF00069	0.313
MOD_GSK3_1	525	532	PF00069	0.337
MOD_GSK3_1	679	686	PF00069	0.376
MOD_GSK3_1	725	732	PF00069	0.302
MOD_GSK3_1	741	748	PF00069	0.302
MOD_GSK3_1	830	837	PF00069	0.394
MOD_GSK3_1	840	847	PF00069	0.375
MOD_N-GLC_1	336	341	PF02516	0.363
MOD_N-GLC_1	448	453	PF02516	0.302
MOD_N-GLC_1	506	511	PF02516	0.297
MOD_N-GLC_1	619	624	PF02516	0.541
MOD_N-GLC_1	829	834	PF02516	0.605
MOD_NEK2_1	1	6	PF00069	0.552
MOD_NEK2_1	22	27	PF00069	0.564
MOD_NEK2_1	244	249	PF00069	0.302
MOD_NEK2_1	273	278	PF00069	0.302
MOD_NEK2_1	333	338	PF00069	0.302
MOD_NEK2_1	355	360	PF00069	0.365
MOD_NEK2_1	371	376	PF00069	0.302
MOD_NEK2_1	506	511	PF00069	0.340
MOD_NEK2_1	55	60	PF00069	0.316
MOD_NEK2_1	66	71	PF00069	0.389
MOD_NEK2_1	676	681	PF00069	0.372
MOD_NEK2_1	729	734	PF00069	0.305
MOD_NEK2_1	834	839	PF00069	0.420
MOD_NEK2_1	842	847	PF00069	0.400
MOD_PIKK_1	363	369	PF00454	0.362
MOD_PKA_2	305	311	PF00069	0.302
MOD_PKA_2	725	731	PF00069	0.302
MOD_PKB_1	869	877	PF00069	0.275
MOD_Plk_1	137	143	PF00069	0.302
MOD_Plk_1	371	377	PF00069	0.323
MOD_Plk_1	430	436	PF00069	0.462
MOD_Plk_1	619	625	PF00069	0.536
MOD_Plk_1	829	835	PF00069	0.362
MOD_Plk_2-3	299	305	PF00069	0.317
MOD_Plk_2-3	326	332	PF00069	0.332
MOD_Plk_2-3	655	661	PF00069	0.458
MOD_Plk_4	210	216	PF00069	0.302
MOD_Plk_4	219	225	PF00069	0.302
MOD_Plk_4	246	252	PF00069	0.302
MOD_Plk_4	345	351	PF00069	0.332
MOD_Plk_4	372	378	PF00069	0.449
MOD_Plk_4	435	441	PF00069	0.302
MOD_Plk_4	451	457	PF00069	0.302
MOD_Plk_4	72	78	PF00069	0.381
MOD_Plk_4	745	751	PF00069	0.444
MOD_Plk_4	770	776	PF00069	0.444
MOD_ProDKin_1	11	17	PF00069	0.462
MOD_ProDKin_1	479	485	PF00069	0.302
MOD_ProDKin_1	560	566	PF00069	0.302
MOD_ProDKin_1	607	613	PF00069	0.380
MOD_ProDKin_1	647	653	PF00069	0.424
MOD_ProDKin_1	683	689	PF00069	0.386
MOD_ProDKin_1	692	698	PF00069	0.380
MOD_SUMO_rev_2	394	401	PF00179	0.453
MOD_SUMO_rev_2	74	81	PF00179	0.245
MOD_SUMO_rev_2	873	881	PF00179	0.377
TRG_DiLeu_BaEn_2	164	170	PF01217	0.302
TRG_ENDOCYTIC_2	322	325	PF00928	0.396
TRG_ENDOCYTIC_2	35	38	PF00928	0.359
TRG_ENDOCYTIC_2	45	48	PF00928	0.335
TRG_ENDOCYTIC_2	511	514	PF00928	0.302
TRG_ENDOCYTIC_2	618	621	PF00928	0.416
TRG_ER_diArg_1	124	126	PF00400	0.195
TRG_ER_diArg_1	869	872	PF00400	0.260
TRG_Pf-PMV_PEXEL_1	53	57	PF00026	0.317

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5H1	Leptomonas seymouri	86%	100%
A0A0S4IZ58	Bodo saltans	74%	100%
A0A0S4J7M0	Bodo saltans	59%	100%
A0A1X0P887	Trypanosomatidae	77%	100%
A0A3Q8IAZ4	Leishmania donovani	95%	100%
A0A3R7RJL9	Trypanosoma rangeli	74%	100%
A0JMA0	Xenopus tropicalis	50%	94%
A0QX20	Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)	50%	95%
A4H9F9	Leishmania braziliensis	89%	100%
A4HXS6	Leishmania infantum	95%	100%
B3VKQ2	Sus scrofa	45%	93%
D0A059	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	74%	100%
O04916	Solanum tuberosum	61%	100%
O08451	Mycobacterium avium	48%	93%
O13966	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
O53166	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	49%	95%
P09339	Bacillus subtilis (strain 168)	53%	99%
P16276	Sus scrofa	27%	100%
P19414	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P20004	Bos taurus	28%	100%
P21399	Homo sapiens	59%	100%
P25516	Escherichia coli (strain K12)	52%	100%
P28271	Mus musculus	58%	100%
P34455	Caenorhabditis elegans	27%	100%
P37032	Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)	54%	100%
P39533	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	100%
P48200	Homo sapiens	45%	93%
P49601	Ustilago maydis (strain 521 / FGSC 9021)	25%	100%
P49608	Cucurbita maxima	62%	100%
P49609	Gracilaria gracilis	29%	100%
P63433	Staphylococcus aureus (strain Mu50 / ATCC 700699)	53%	99%
P63434	Staphylococcus aureus (strain MW2)	53%	99%
P70920	Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110)	53%	99%
P82611	Candida albicans (strain SC5314 / ATCC MYA-2876)	27%	100%
P99148	Staphylococcus aureus (strain N315)	53%	99%
Q01059	Oryctolagus cuniculus	59%	100%
Q0VCU1	Bos taurus	58%	100%
Q1RKD5	Rickettsia bellii (strain RML369-C)	53%	100%
Q23500	Caenorhabditis elegans	60%	100%
Q2A1K3	Francisella tularensis subsp. holarctica (strain LVS)	52%	96%
Q42560	Arabidopsis thaliana	62%	100%
Q42669	Cucumis melo var. conomon	61%	100%
Q4JVM4	Corynebacterium jeikeium (strain K411)	50%	96%
Q4QDZ1	Leishmania major	95%	100%
Q4UK20	Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)	53%	100%
Q4WJ90	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	27%	100%
Q4WLN1	Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)	28%	100%
Q54X73	Dictyostelium discoideum	59%	100%
Q54XS2	Dictyostelium discoideum	27%	100%
Q59938	Streptococcus mutans serotype c (strain ATCC 700610 / UA159)	53%	100%
Q5B6D6	Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)	27%	100%
Q5HG69	Staphylococcus aureus (strain COL)	53%	99%
Q5HPJ0	Staphylococcus epidermidis (strain ATCC 35984 / RP62A)	54%	99%
Q5SMF6	Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)	56%	99%
Q5ZLQ4	Gallus gallus	50%	93%
Q62751	Rattus norvegicus	44%	93%
Q63270	Rattus norvegicus	58%	100%
Q68VV0	Rickettsia typhi (strain ATCC VR-144 / Wilmington)	53%	100%
Q6G9K9	Staphylococcus aureus (strain MSSA476)	53%	99%
Q6GH55	Staphylococcus aureus (strain MRSA252)	53%	99%
Q6NH63	Corynebacterium diphtheriae (strain ATCC 700971 / NCTC 13129 / Biotype gravis)	49%	96%
Q6NTP2	Xenopus laevis	50%	94%
Q6YZX6	Oryza sativa subsp. japonica	62%	100%
Q811J3	Mus musculus	44%	93%
Q8CPC2	Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200)	54%	99%
Q8EJW3	Shewanella oneidensis (strain MR-1)	42%	100%
Q8FTA8	Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)	50%	96%
Q8NQ98	Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)	49%	95%
Q8ZP52	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	53%	100%
Q90875	Gallus gallus	59%	100%
Q92G90	Rickettsia conorii (strain ATCC VR-613 / Malish 7)	53%	100%
Q937N8	Cupriavidus necator	43%	100%
Q94A28	Arabidopsis thaliana	62%	90%
Q99798	Homo sapiens	27%	100%
Q99KI0	Mus musculus	27%	100%
Q9ER34	Rattus norvegicus	27%	100%
Q9I3F5	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	53%	98%
Q9P7D4	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	98%
Q9RTN7	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	55%	99%
Q9SIB9	Arabidopsis thaliana	62%	91%
Q9ZCF4	Rickettsia prowazekii (strain Madrid E)	52%	100%
V5B6G9	Trypanosoma cruzi	76%	94%