GPI-anchor transamidase subunit 8 (GPI8),putative

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cysteine peptidase, Clan CD, family C13, putative

Species:

Leishmania mexicana

UniProt:

E9ARH3_LEIMU

TriTrypDb:

LmxM.18.0360

Length:

342

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 5, no: 4
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0008303	caspase complex	5	10
GO:0032991	protein-containing complex	1	10
GO:0042765	GPI-anchor transamidase complex	3	10
GO:0098796	membrane protein complex	2	10
GO:0140534	endoplasmic reticulum protein-containing complex	2	10
GO:1902494	catalytic complex	2	10
GO:1905368	peptidase complex	3	10
GO:1905369	endopeptidase complex	4	10
GO:0005635	nuclear envelope	4	1
GO:0005783	endoplasmic reticulum	5	1
GO:0031967	organelle envelope	3	1
GO:0031975	envelope	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9ARH3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARH3

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	10
GO:0006807	nitrogen compound metabolic process	2	10
GO:0008152	metabolic process	1	10
GO:0016255	attachment of GPI anchor to protein	5	10
GO:0019538	protein metabolic process	3	10
GO:0036211	protein modification process	4	10
GO:0043170	macromolecule metabolic process	3	10
GO:0043412	macromolecule modification	4	10
GO:0044238	primary metabolic process	2	10
GO:0071704	organic substance metabolic process	2	10
GO:1901564	organonitrogen compound metabolic process	3	10
GO:0008104	protein localization	4	1
GO:0009987	cellular process	1	1
GO:0033036	macromolecule localization	2	1
GO:0034394	protein localization to cell surface	5	1
GO:0051179	localization	1	1
GO:0051641	cellular localization	2	1
GO:0070727	cellular macromolecule localization	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0003923	GPI-anchor transamidase activity	6	10
GO:0004175	endopeptidase activity	4	10
GO:0004197	cysteine-type endopeptidase activity	5	10
GO:0008233	peptidase activity	3	10
GO:0008234	cysteine-type peptidase activity	4	10
GO:0016787	hydrolase activity	2	10
GO:0140096	catalytic activity, acting on a protein	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	132	134	PF00675	0.317
CLV_NRD_NRD_1	150	152	PF00675	0.318
CLV_NRD_NRD_1	197	199	PF00675	0.318
CLV_NRD_NRD_1	271	273	PF00675	0.654
CLV_PCSK_KEX2_1	132	134	PF00082	0.317
CLV_PCSK_KEX2_1	150	152	PF00082	0.318
CLV_PCSK_KEX2_1	197	199	PF00082	0.318
CLV_PCSK_KEX2_1	271	273	PF00082	0.612
CLV_PCSK_SKI1_1	132	136	PF00082	0.300
CLV_PCSK_SKI1_1	150	154	PF00082	0.308
CLV_PCSK_SKI1_1	201	205	PF00082	0.318
CLV_PCSK_SKI1_1	49	53	PF00082	0.318
DEG_APCC_DBOX_1	131	139	PF00400	0.318
DOC_CYCLIN_RxL_1	147	157	PF00134	0.418
DOC_MAPK_gen_1	197	205	PF00069	0.318
DOC_PP1_RVXF_1	199	205	PF00149	0.320
DOC_PP4_FxxP_1	98	101	PF00568	0.403
DOC_USP7_MATH_1	24	28	PF00917	0.622
DOC_USP7_MATH_2	94	100	PF00917	0.382
DOC_USP7_UBL2_3	170	174	PF12436	0.418
DOC_WW_Pin1_4	143	148	PF00397	0.318
LIG_14-3-3_CanoR_1	110	114	PF00244	0.168
LIG_14-3-3_CanoR_1	132	138	PF00244	0.413
LIG_14-3-3_CanoR_1	150	160	PF00244	0.234
LIG_14-3-3_CanoR_1	55	63	PF00244	0.338
LIG_14-3-3_CanoR_1	6	15	PF00244	0.586
LIG_BIR_III_4	106	110	PF00653	0.284
LIG_BRCT_BRCA1_1	81	85	PF00533	0.418
LIG_deltaCOP1_diTrp_1	318	324	PF00928	0.420
LIG_FHA_1	147	153	PF00498	0.410
LIG_FHA_1	155	161	PF00498	0.168
LIG_FHA_1	176	182	PF00498	0.382
LIG_FHA_1	244	250	PF00498	0.382
LIG_FHA_1	268	274	PF00498	0.620
LIG_FHA_1	3	9	PF00498	0.577
LIG_LIR_Apic_2	96	101	PF02991	0.354
LIG_LIR_Gen_1	38	45	PF02991	0.297
LIG_LIR_Gen_1	62	72	PF02991	0.418
LIG_LIR_Nem_3	171	175	PF02991	0.331
LIG_LIR_Nem_3	255	260	PF02991	0.351
LIG_LIR_Nem_3	284	288	PF02991	0.358
LIG_LIR_Nem_3	38	44	PF02991	0.297
LIG_LIR_Nem_3	62	67	PF02991	0.378
LIG_SH2_CRK	199	203	PF00017	0.300
LIG_SH2_CRK	64	68	PF00017	0.418
LIG_SH2_STAP1	236	240	PF00017	0.343
LIG_SH2_STAT3	236	239	PF00017	0.403
LIG_SH2_STAT3	240	243	PF00017	0.403
LIG_SH2_STAT5	163	166	PF00017	0.382
LIG_SH2_STAT5	172	175	PF00017	0.389
LIG_SH2_STAT5	257	260	PF00017	0.295
LIG_SH2_STAT5	50	53	PF00017	0.316
LIG_SH3_3	215	221	PF00018	0.459
LIG_SH3_3	307	313	PF00018	0.421
LIG_Sin3_3	9	16	PF02671	0.519
LIG_SUMO_SIM_anti_2	275	282	PF11976	0.560
LIG_WRC_WIRS_1	248	253	PF05994	0.403
MOD_CDC14_SPxK_1	3	6	PF00782	0.468
MOD_CDK_SPxxK_3	143	150	PF00069	0.318
MOD_CK1_1	154	160	PF00069	0.168
MOD_CK1_1	184	190	PF00069	0.459
MOD_CK1_1	327	333	PF00069	0.350
MOD_CK2_1	226	232	PF00069	0.418
MOD_GlcNHglycan	118	121	PF01048	0.413
MOD_GlcNHglycan	26	29	PF01048	0.633
MOD_GlcNHglycan	329	332	PF01048	0.370
MOD_GSK3_1	151	158	PF00069	0.318
MOD_GSK3_1	175	182	PF00069	0.343
MOD_GSK3_1	184	191	PF00069	0.343
MOD_GSK3_1	226	233	PF00069	0.420
MOD_GSK3_1	243	250	PF00069	0.448
MOD_GSK3_1	263	270	PF00069	0.596
MOD_GSK3_1	295	302	PF00069	0.605
MOD_N-GLC_1	155	160	PF02516	0.168
MOD_N-GLC_1	260	265	PF02516	0.469
MOD_N-GLC_1	299	304	PF02516	0.534
MOD_NEK2_1	18	23	PF00069	0.596
MOD_NEK2_1	205	210	PF00069	0.318
MOD_NEK2_1	260	265	PF00069	0.440
MOD_NEK2_1	281	286	PF00069	0.358
MOD_NEK2_1	30	35	PF00069	0.596
MOD_NEK2_1	324	329	PF00069	0.259
MOD_NEK2_1	81	86	PF00069	0.375
MOD_PIKK_1	324	330	PF00454	0.272
MOD_PIKK_1	96	102	PF00454	0.382
MOD_PK_1	133	139	PF00069	0.418
MOD_PKA_2	109	115	PF00069	0.168
MOD_PKA_2	54	60	PF00069	0.338
MOD_Plk_4	133	139	PF00069	0.418
MOD_Plk_4	168	174	PF00069	0.359
MOD_Plk_4	188	194	PF00069	0.129
MOD_Plk_4	247	253	PF00069	0.382
MOD_Plk_4	276	282	PF00069	0.436
MOD_Plk_4	59	65	PF00069	0.343
MOD_ProDKin_1	143	149	PF00069	0.318
TRG_DiLeu_BaEn_1	255	260	PF01217	0.355
TRG_DiLeu_BaLyEn_6	148	153	PF01217	0.418
TRG_ENDOCYTIC_2	172	175	PF00928	0.371
TRG_ENDOCYTIC_2	199	202	PF00928	0.299
TRG_ENDOCYTIC_2	257	260	PF00928	0.459
TRG_ENDOCYTIC_2	64	67	PF00928	0.418
TRG_ER_diArg_1	131	133	PF00400	0.317
TRG_ER_diArg_1	150	152	PF00400	0.318
TRG_ER_diArg_1	197	199	PF00400	0.318
TRG_ER_diArg_1	271	273	PF00400	0.654
TRG_NES_CRM1_1	242	255	PF08389	0.377
TRG_Pf-PMV_PEXEL_1	150	155	PF00026	0.318
TRG_Pf-PMV_PEXEL_1	271	275	PF00026	0.367

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ31	Leptomonas seymouri	79%	94%
A0A1X0P6S3	Trypanosomatidae	52%	100%
A0A3S5H727	Leishmania donovani	95%	96%
A4H9E0	Leishmania braziliensis	83%	100%
A4HXR1	Leishmania infantum	95%	96%
B6DTF5	Bodo saltans	39%	100%
D0A043	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
O89017	Mus musculus	27%	79%
Q3MHZ7	Bos taurus	40%	87%
Q4KRV1	Sus scrofa	38%	87%
Q4QE06	Leishmania major	88%	100%
Q4R4T8	Macaca fascicularis	28%	79%
Q5R5D9	Pongo abelii	27%	79%
Q5R6L8	Pongo abelii	40%	87%
Q8T4E1	Drosophila melanogaster	33%	96%
Q92643	Homo sapiens	40%	87%
Q95M12	Bos taurus	28%	79%
Q99538	Homo sapiens	27%	79%
Q9CXY9	Mus musculus	40%	87%
Q9LJX8	Arabidopsis thaliana	26%	73%
Q9R0J8	Rattus norvegicus	27%	79%
Q9USP5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	90%
V5BWA3	Trypanosoma cruzi	53%	100%