Elongation factor 1-alpha

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

elongation factor 1-alpha

Species:

Leishmania mexicana

UniProt:

E9ARD0_LEIMU

TriTrypDb:

LmxM.17.0080 , LmxM.17.0081 , LmxM.17.0082

Length:

449

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: ethan@interchange.ubc.ca
Publication title: Leishmania EF-1alpha Activates the Src Homology 2 Domain Containing Tyrosine Phosphatase SHP-1 Leading to Macrophage Deactivation
Publication 1st author(s): D. Nandan
Publication Identifier(s): 12384497
Host organism: Mus musculus
Interaction detection method(s): coimmunoprecipitation
Interaction type: direct interaction
Identification method participant A: western blot
Identification method participant B: predetermined participant
ID(s) interactor A: Q95VF2
ID(s) interactor B: P29350
Taxid interactor A: Leishmania donovani
Taxid interactor B: Mus musculus
Biological role(s) interactor A: enzyme target
Biological role(s) interactor B: enzyme
Experimental role(s) interactor A: prey

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 27

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 10

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

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Cellular components
Term	Name	Level	Count
GO:0018995	host cellular component	2	1
GO:0033643	host cell part	3	1
GO:0033646	host intracellular part	4	1
GO:0033655	host cell cytoplasm part	5	1
GO:0044164	host cell cytosol	6	1
GO:0110165	cellular anatomical entity	1	1

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Expansion

Sequence features

E9ARD0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARD0

Function

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Biological processes
Term	Name	Level	Count
GO:0006412	translation	4	2
GO:0006414	translational elongation	5	3
GO:0006518	peptide metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	3
GO:0009058	biosynthetic process	2	3
GO:0009059	macromolecule biosynthetic process	4	3
GO:0009605	response to external stimulus	2	1
GO:0009607	response to biotic stimulus	2	1
GO:0009893	positive regulation of metabolic process	4	1

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Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0003676	nucleic acid binding	3	16
GO:0003746	translation elongation factor activity	4	16
GO:0003824	catalytic activity	1	16
GO:0003924	GTPase activity	7	16
GO:0005488	binding	1	16
GO:0005515	protein binding	2	1
GO:0005516	calmodulin binding	3	1
GO:0005525	GTP binding	5	16
GO:0008135	translation factor activity, RNA binding	3	16

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Putative motif mimicry

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Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	107	111	PF00656	0.443
CLV_C14_Caspase3-7	203	207	PF00656	0.594
CLV_C14_Caspase3-7	215	219	PF00656	0.607
CLV_C14_Caspase3-7	88	92	PF00656	0.507
CLV_NRD_NRD_1	309	311	PF00675	0.310
CLV_NRD_NRD_1	369	371	PF00675	0.310
CLV_PCSK_KEX2_1	179	181	PF00082	0.409
CLV_PCSK_KEX2_1	309	311	PF00082	0.310
CLV_PCSK_KEX2_1	36	38	PF00082	0.309
CLV_PCSK_KEX2_1	369	371	PF00082	0.310

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Homologs

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Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2Q3	Leptomonas seymouri	28%	96%
A0A0N1I5B5	Leptomonas seymouri	35%	69%
A0A0S4IUT7	Bodo saltans	82%	100%
A0A0S4IX91	Bodo saltans	36%	67%
A0A0S4J558	Bodo saltans	29%	96%
A0A0S4JRG2	Bodo saltans	84%	100%
A0A1X0NXE4	Trypanosomatidae	93%	100%
A0A1X0NXE5	Trypanosomatidae	94%	100%
A0A1X0P591	Trypanosomatidae	34%	67%
A0A1X0P746	Trypanosomatidae	29%	96%

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