Separase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Separase

Gene product:

eukaryotic translation initiation factor 3 subunit a

Species:

Leishmania mexicana

UniProt:

E9ARC2_LEIMU

TriTrypDb:

LmxM.17.0010

Length:

774

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 4

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 4

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005852	eukaryotic translation initiation factor 3 complex	2	12
GO:0032991	protein-containing complex	1	12
GO:0005737	cytoplasm	2	1
GO:0043614	multi-eIF complex	3	1
GO:0071540	eukaryotic translation initiation factor 3 complex, eIF3e	3	1
GO:0071541	eukaryotic translation initiation factor 3 complex, eIF3m	3	1
GO:0110165	cellular anatomical entity	1	1
GO:1990904	ribonucleoprotein complex	2	1

Expansion

Sequence features

E9ARC2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9ARC2

Function

Biological processes
Term	Name	Level	Count
GO:0001732	formation of cytoplasmic translation initiation complex	7	1
GO:0002183	cytoplasmic translational initiation	4	1
GO:0002188	translation reinitiation	5	1
GO:0006413	translational initiation	3	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0022618	ribonucleoprotein complex assembly	6	1
GO:0043933	protein-containing complex organization	4	1
GO:0044237	cellular metabolic process	2	1
GO:0065003	protein-containing complex assembly	5	1
GO:0071826	ribonucleoprotein complex subunit organization	5	1
GO:0071840	cellular component organization or biogenesis	2	1

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003743	translation initiation factor activity	4	11
GO:0005488	binding	1	11
GO:0008135	translation factor activity, RNA binding	3	11
GO:0045182	translation regulator activity	1	11
GO:0090079	translation regulator activity, nucleic acid binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0003723	RNA binding	4	1
GO:0003729	mRNA binding	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	490	494	PF00656	0.593
CLV_NRD_NRD_1	151	153	PF00675	0.575
CLV_NRD_NRD_1	590	592	PF00675	0.635
CLV_NRD_NRD_1	606	608	PF00675	0.438
CLV_NRD_NRD_1	626	628	PF00675	0.319
CLV_NRD_NRD_1	663	665	PF00675	0.555
CLV_NRD_NRD_1	669	671	PF00675	0.573
CLV_NRD_NRD_1	690	692	PF00675	0.522
CLV_NRD_NRD_1	718	720	PF00675	0.499
CLV_NRD_NRD_1	723	725	PF00675	0.482
CLV_NRD_NRD_1	74	76	PF00675	0.503
CLV_NRD_NRD_1	745	747	PF00675	0.721
CLV_NRD_NRD_1	769	771	PF00675	0.663
CLV_PCSK_KEX2_1	140	142	PF00082	0.464
CLV_PCSK_KEX2_1	151	153	PF00082	0.333
CLV_PCSK_KEX2_1	272	274	PF00082	0.500
CLV_PCSK_KEX2_1	319	321	PF00082	0.594
CLV_PCSK_KEX2_1	323	325	PF00082	0.323
CLV_PCSK_KEX2_1	537	539	PF00082	0.629
CLV_PCSK_KEX2_1	582	584	PF00082	0.557
CLV_PCSK_KEX2_1	590	592	PF00082	0.640
CLV_PCSK_KEX2_1	662	664	PF00082	0.524
CLV_PCSK_KEX2_1	690	692	PF00082	0.505
CLV_PCSK_KEX2_1	718	720	PF00082	0.520
CLV_PCSK_KEX2_1	723	725	PF00082	0.471
CLV_PCSK_KEX2_1	74	76	PF00082	0.529
CLV_PCSK_KEX2_1	747	749	PF00082	0.587
CLV_PCSK_KEX2_1	756	758	PF00082	0.493
CLV_PCSK_KEX2_1	769	771	PF00082	0.517
CLV_PCSK_PC1ET2_1	140	142	PF00082	0.464
CLV_PCSK_PC1ET2_1	272	274	PF00082	0.500
CLV_PCSK_PC1ET2_1	319	321	PF00082	0.594
CLV_PCSK_PC1ET2_1	323	325	PF00082	0.323
CLV_PCSK_PC1ET2_1	537	539	PF00082	0.743
CLV_PCSK_PC1ET2_1	582	584	PF00082	0.573
CLV_PCSK_PC1ET2_1	662	664	PF00082	0.534
CLV_PCSK_PC1ET2_1	690	692	PF00082	0.524
CLV_PCSK_PC1ET2_1	747	749	PF00082	0.587
CLV_PCSK_PC1ET2_1	756	758	PF00082	0.493
CLV_PCSK_PC7_1	719	725	PF00082	0.624
CLV_PCSK_PC7_1	743	749	PF00082	0.537
CLV_PCSK_SKI1_1	167	171	PF00082	0.471
CLV_PCSK_SKI1_1	178	182	PF00082	0.390
CLV_PCSK_SKI1_1	231	235	PF00082	0.464
CLV_PCSK_SKI1_1	3	7	PF00082	0.665
CLV_PCSK_SKI1_1	330	334	PF00082	0.373
CLV_PCSK_SKI1_1	409	413	PF00082	0.514
CLV_PCSK_SKI1_1	430	434	PF00082	0.449
CLV_PCSK_SKI1_1	538	542	PF00082	0.550
CLV_PCSK_SKI1_1	623	627	PF00082	0.438
CLV_PCSK_SKI1_1	671	675	PF00082	0.617
CLV_PCSK_SKI1_1	728	732	PF00082	0.519
CLV_Separin_Metazoa	624	628	PF03568	0.566
CLV_Separin_Metazoa	71	75	PF03568	0.544
DEG_APCC_DBOX_1	329	337	PF00400	0.379
DEG_APCC_DBOX_1	433	441	PF00400	0.497
DEG_Nend_UBRbox_2	1	3	PF02207	0.725
DEG_SCF_FBW7_2	540	545	PF00400	0.444
DEG_SPOP_SBC_1	532	536	PF00917	0.616
DEG_SPOP_SBC_1	552	556	PF00917	0.345
DOC_CKS1_1	42	47	PF01111	0.474
DOC_CKS1_1	539	544	PF01111	0.456
DOC_MAPK_DCC_7	39	48	PF00069	0.490
DOC_MAPK_gen_1	109	117	PF00069	0.569
DOC_MAPK_gen_1	277	287	PF00069	0.468
DOC_MAPK_MEF2A_6	92	101	PF00069	0.439
DOC_MAPK_RevD_3	127	141	PF00069	0.460
DOC_PP1_RVXF_1	165	172	PF00149	0.531
DOC_PP2B_LxvP_1	686	689	PF13499	0.460
DOC_PP4_FxxP_1	187	190	PF00568	0.520
DOC_PP4_FxxP_1	362	365	PF00568	0.603
DOC_USP7_MATH_1	11	15	PF00917	0.566
DOC_USP7_MATH_1	33	37	PF00917	0.462
DOC_USP7_MATH_1	4	8	PF00917	0.670
DOC_USP7_MATH_1	416	420	PF00917	0.632
DOC_USP7_MATH_1	514	518	PF00917	0.698
DOC_USP7_MATH_1	519	523	PF00917	0.713
DOC_USP7_MATH_1	532	536	PF00917	0.729
DOC_USP7_MATH_1	575	579	PF00917	0.545
DOC_USP7_MATH_1	704	708	PF00917	0.424
DOC_USP7_UBL2_3	319	323	PF12436	0.593
DOC_USP7_UBL2_3	577	581	PF12436	0.638
DOC_USP7_UBL2_3	628	632	PF12436	0.491
DOC_WW_Pin1_4	361	366	PF00397	0.622
DOC_WW_Pin1_4	383	388	PF00397	0.558
DOC_WW_Pin1_4	41	46	PF00397	0.492
DOC_WW_Pin1_4	538	543	PF00397	0.565
LIG_14-3-3_CanoR_1	10	16	PF00244	0.581
LIG_14-3-3_CanoR_1	178	184	PF00244	0.475
LIG_14-3-3_CanoR_1	202	209	PF00244	0.449
LIG_14-3-3_CanoR_1	485	490	PF00244	0.573
LIG_14-3-3_CanoR_1	524	531	PF00244	0.641
LIG_14-3-3_CanoR_1	551	560	PF00244	0.551
LIG_Actin_WH2_2	65	83	PF00022	0.554
LIG_AP2alpha_2	346	348	PF02296	0.656
LIG_BRCT_BRCA1_1	295	299	PF00533	0.487
LIG_FHA_1	236	242	PF00498	0.423
LIG_FHA_1	299	305	PF00498	0.593
LIG_FHA_1	368	374	PF00498	0.579
LIG_FHA_1	376	382	PF00498	0.496
LIG_FHA_1	484	490	PF00498	0.487
LIG_FHA_1	542	548	PF00498	0.643
LIG_FHA_1	653	659	PF00498	0.436
LIG_FHA_2	118	124	PF00498	0.580
LIG_FHA_2	292	298	PF00498	0.490
LIG_FHA_2	458	464	PF00498	0.550
LIG_IRF3_LxIS_1	190	197	PF10401	0.421
LIG_LIR_Apic_2	186	190	PF02991	0.471
LIG_LIR_Apic_2	359	365	PF02991	0.595
LIG_LIR_Gen_1	282	292	PF02991	0.530
LIG_LIR_Gen_1	398	407	PF02991	0.528
LIG_LIR_Gen_1	443	454	PF02991	0.597
LIG_LIR_Gen_1	501	510	PF02991	0.516
LIG_LIR_Gen_1	522	533	PF02991	0.724
LIG_LIR_Nem_3	282	287	PF02991	0.606
LIG_LIR_Nem_3	346	351	PF02991	0.681
LIG_LIR_Nem_3	398	404	PF02991	0.523
LIG_LIR_Nem_3	443	449	PF02991	0.593
LIG_LIR_Nem_3	501	506	PF02991	0.523
LIG_LIR_Nem_3	522	528	PF02991	0.722
LIG_NRBOX	247	253	PF00104	0.491
LIG_NRBOX	653	659	PF00104	0.473
LIG_PCNA_yPIPBox_3	167	181	PF02747	0.506
LIG_PCNA_yPIPBox_3	350	362	PF02747	0.519
LIG_PCNA_yPIPBox_3	91	102	PF02747	0.441
LIG_Pex14_2	741	745	PF04695	0.639
LIG_RPA_C_Fungi	548	560	PF08784	0.617
LIG_SH2_GRB2like	464	467	PF00017	0.516
LIG_SH2_GRB2like	502	505	PF00017	0.538
LIG_SH2_NCK_1	382	386	PF00017	0.400
LIG_SH2_PTP2	446	449	PF00017	0.466
LIG_SH2_SRC	446	449	PF00017	0.582
LIG_SH2_STAP1	679	683	PF00017	0.599
LIG_SH2_STAT3	558	561	PF00017	0.609
LIG_SH2_STAT3	694	697	PF00017	0.559
LIG_SH2_STAT5	310	313	PF00017	0.430
LIG_SH2_STAT5	382	385	PF00017	0.399
LIG_SH2_STAT5	446	449	PF00017	0.498
LIG_SH2_STAT5	464	467	PF00017	0.359
LIG_SH2_STAT5	502	505	PF00017	0.538
LIG_SH2_STAT5	605	608	PF00017	0.592
LIG_SH2_STAT5	713	716	PF00017	0.626
LIG_SUMO_SIM_anti_2	282	289	PF11976	0.620
LIG_SUMO_SIM_anti_2	49	56	PF11976	0.513
LIG_SUMO_SIM_par_1	192	198	PF11976	0.501
LIG_SUMO_SIM_par_1	469	477	PF11976	0.534
LIG_TRAF2_1	223	226	PF00917	0.571
LIG_UBA3_1	372	379	PF00899	0.517
MOD_CK1_1	13	19	PF00069	0.592
MOD_CK1_1	198	204	PF00069	0.491
MOD_CK1_1	239	245	PF00069	0.372
MOD_CK1_1	364	370	PF00069	0.595
MOD_CK1_1	402	408	PF00069	0.467
MOD_CK1_1	517	523	PF00069	0.737
MOD_CK1_1	526	532	PF00069	0.778
MOD_CK2_1	457	463	PF00069	0.560
MOD_CK2_1	564	570	PF00069	0.530
MOD_GlcNHglycan	197	200	PF01048	0.363
MOD_GlcNHglycan	238	241	PF01048	0.395
MOD_GlcNHglycan	288	291	PF01048	0.459
MOD_GlcNHglycan	440	443	PF01048	0.524
MOD_GlcNHglycan	521	524	PF01048	0.637
MOD_GlcNHglycan	645	648	PF01048	0.601
MOD_GlcNHglycan	705	709	PF01048	0.398
MOD_GlcNHglycan	77	80	PF01048	0.520
MOD_GSK3_1	194	201	PF00069	0.438
MOD_GSK3_1	235	242	PF00069	0.472
MOD_GSK3_1	310	317	PF00069	0.482
MOD_GSK3_1	361	368	PF00069	0.553
MOD_GSK3_1	412	419	PF00069	0.575
MOD_GSK3_1	481	488	PF00069	0.477
MOD_GSK3_1	509	516	PF00069	0.695
MOD_GSK3_1	519	526	PF00069	0.746
MOD_N-GLC_1	117	122	PF02516	0.586
MOD_N-GLC_1	159	164	PF02516	0.456
MOD_N-GLC_1	173	178	PF02516	0.383
MOD_N-GLC_1	399	404	PF02516	0.589
MOD_N-GLC_1	457	462	PF02516	0.644
MOD_NEK2_1	194	199	PF00069	0.434
MOD_NEK2_1	234	239	PF00069	0.484
MOD_NEK2_1	407	412	PF00069	0.510
MOD_NEK2_1	440	445	PF00069	0.517
MOD_NEK2_1	491	496	PF00069	0.569
MOD_NEK2_1	518	523	PF00069	0.690
MOD_NEK2_1	741	746	PF00069	0.644
MOD_NEK2_1	80	85	PF00069	0.502
MOD_NEK2_2	173	178	PF00069	0.491
MOD_NEK2_2	300	305	PF00069	0.437
MOD_PIKK_1	57	63	PF00454	0.403
MOD_PIKK_1	598	604	PF00454	0.623
MOD_PIKK_1	80	86	PF00454	0.489
MOD_PK_1	553	559	PF00069	0.525
MOD_PKA_1	167	173	PF00069	0.523
MOD_PKA_2	11	17	PF00069	0.548
MOD_PKA_2	491	497	PF00069	0.619
MOD_PKA_2	523	529	PF00069	0.721
MOD_PKA_2	552	558	PF00069	0.499
MOD_PKA_2	80	86	PF00069	0.493
MOD_PKB_1	551	559	PF00069	0.529
MOD_Plk_1	159	165	PF00069	0.458
MOD_Plk_1	173	179	PF00069	0.387
MOD_Plk_1	474	480	PF00069	0.578
MOD_Plk_1	526	532	PF00069	0.637
MOD_Plk_1	652	658	PF00069	0.377
MOD_Plk_1	741	747	PF00069	0.561
MOD_Plk_1	86	92	PF00069	0.469
MOD_Plk_2-3	476	482	PF00069	0.592
MOD_Plk_4	167	173	PF00069	0.499
MOD_Plk_4	300	306	PF00069	0.439
MOD_Plk_4	368	374	PF00069	0.502
MOD_Plk_4	498	504	PF00069	0.524
MOD_Plk_4	553	559	PF00069	0.504
MOD_Plk_4	93	99	PF00069	0.542
MOD_ProDKin_1	361	367	PF00069	0.617
MOD_ProDKin_1	383	389	PF00069	0.549
MOD_ProDKin_1	41	47	PF00069	0.483
MOD_ProDKin_1	538	544	PF00069	0.561
MOD_SUMO_for_1	596	599	PF00179	0.541
MOD_SUMO_rev_2	20	30	PF00179	0.509
MOD_SUMO_rev_2	570	578	PF00179	0.633
MOD_SUMO_rev_2	667	673	PF00179	0.626
TRG_DiLeu_BaEn_1	49	54	PF01217	0.503
TRG_DiLeu_BaEn_1	65	70	PF01217	0.379
TRG_DiLeu_BaEn_1	653	658	PF01217	0.446
TRG_DiLeu_BaEn_1	759	764	PF01217	0.515
TRG_DiLeu_BaEn_2	229	235	PF01217	0.544
TRG_DiLeu_BaEn_4	766	772	PF01217	0.657
TRG_DiLeu_BaLyEn_6	149	154	PF01217	0.499
TRG_DiLeu_LyEn_5	653	658	PF01217	0.558
TRG_DiLeu_LyEn_5	759	764	PF01217	0.660
TRG_ENDOCYTIC_2	292	295	PF00928	0.400
TRG_ENDOCYTIC_2	446	449	PF00928	0.590
TRG_ENDOCYTIC_2	525	528	PF00928	0.722
TRG_ENDOCYTIC_2	633	636	PF00928	0.461
TRG_ER_diArg_1	150	152	PF00400	0.432
TRG_ER_diArg_1	73	75	PF00400	0.543
TRG_ER_diArg_1	745	748	PF00400	0.743
TRG_ER_diArg_1	768	770	PF00400	0.658
TRG_ER_diArg_1	99	102	PF00400	0.563
TRG_NES_CRM1_1	49	62	PF08389	0.519
TRG_NLS_MonoExtC_3	661	667	PF00514	0.600
TRG_NLS_MonoExtN_4	743	750	PF00514	0.540
TRG_Pf-PMV_PEXEL_1	607	612	PF00026	0.517
TRG_Pf-PMV_PEXEL_1	762	766	PF00026	0.653
TRG_Pf-PMV_PEXEL_1	769	774	PF00026	0.677

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IGW0	Leptomonas seymouri	76%	100%
A0A0S4IU09	Bodo saltans	22%	95%
A0A1X0NTC2	Trypanosomatidae	34%	100%
A0A3Q8IDU4	Leishmania donovani	97%	100%
A0A422NFC9	Trypanosoma rangeli	32%	100%
A4H8U7	Leishmania braziliensis	92%	100%
A4HX65	Leishmania infantum	97%	100%
C9ZTS8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
Q4QEJ8	Leishmania major	97%	100%
V5D8P7	Trypanosoma cruzi	32%	100%