VASt domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9AR37_LEIMU

TriTrypDb:

LmxM.17.0740

Length:

352

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10
GO:0005789	endoplasmic reticulum membrane	4	1
GO:0005886	plasma membrane	3	1
GO:0031090	organelle membrane	3	1
GO:0044232	organelle membrane contact site	2	1
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	1

Expansion

Sequence features

E9AR37

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AR37

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006869	lipid transport	5	1
GO:0009987	cellular process	1	1
GO:0015850	organic hydroxy compound transport	5	1
GO:0015918	sterol transport	6	1
GO:0032365	intracellular lipid transport	4	1
GO:0032366	intracellular sterol transport	5	1
GO:0046907	intracellular transport	3	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0051641	cellular localization	2	1
GO:0051649	establishment of localization in cell	3	1
GO:0071702	organic substance transport	4	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	1
GO:0005319	lipid transporter activity	2	1
GO:0005488	binding	1	1
GO:0005496	steroid binding	3	1
GO:0008289	lipid binding	2	1
GO:0015248	sterol transporter activity	3	1
GO:0032934	sterol binding	4	1
GO:0097159	organic cyclic compound binding	2	1
GO:0120013	lipid transfer activity	3	1
GO:0120015	sterol transfer activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	201	205	PF00656	0.584
CLV_NRD_NRD_1	258	260	PF00675	0.540
CLV_NRD_NRD_1	78	80	PF00675	0.242
CLV_PCSK_KEX2_1	258	260	PF00082	0.414
CLV_PCSK_SKI1_1	102	106	PF00082	0.262
CLV_PCSK_SKI1_1	125	129	PF00082	0.270
CLV_PCSK_SKI1_1	325	329	PF00082	0.474
CLV_PCSK_SKI1_1	55	59	PF00082	0.284
DEG_APCC_DBOX_1	124	132	PF00400	0.497
DEG_APCC_DBOX_1	324	332	PF00400	0.206
DOC_CKS1_1	45	50	PF01111	0.609
DOC_CYCLIN_yCln2_LP_2	45	51	PF00134	0.498
DOC_MAPK_DCC_7	43	51	PF00069	0.518
DOC_MAPK_gen_1	299	308	PF00069	0.360
DOC_MAPK_MEF2A_6	130	139	PF00069	0.516
DOC_MAPK_MEF2A_6	242	249	PF00069	0.615
DOC_MAPK_MEF2A_6	325	333	PF00069	0.206
DOC_MAPK_RevD_3	245	259	PF00069	0.541
DOC_USP7_MATH_1	218	222	PF00917	0.624
DOC_WW_Pin1_4	241	246	PF00397	0.679
DOC_WW_Pin1_4	248	253	PF00397	0.600
DOC_WW_Pin1_4	44	49	PF00397	0.618
LIG_14-3-3_CanoR_1	102	110	PF00244	0.457
LIG_14-3-3_CanoR_1	192	202	PF00244	0.522
LIG_Actin_WH2_2	264	280	PF00022	0.457
LIG_AP2alpha_1	150	154	PF02296	0.499
LIG_BIR_II_1	1	5	PF00653	0.592
LIG_CaM_NSCaTE_8	317	324	PF13499	0.418
LIG_deltaCOP1_diTrp_1	83	89	PF00928	0.523
LIG_eIF4E_1	123	129	PF01652	0.497
LIG_FHA_1	105	111	PF00498	0.459
LIG_FHA_1	134	140	PF00498	0.456
LIG_FHA_1	263	269	PF00498	0.472
LIG_FHA_1	281	287	PF00498	0.317
LIG_FHA_1	67	73	PF00498	0.536
LIG_FHA_2	4	10	PF00498	0.610
LIG_IRF3_LxIS_1	331	337	PF10401	0.413
LIG_LIR_Gen_1	22	31	PF02991	0.557
LIG_LIR_Gen_1	279	289	PF02991	0.507
LIG_LIR_Gen_1	36	46	PF02991	0.513
LIG_LIR_Nem_3	122	126	PF02991	0.486
LIG_LIR_Nem_3	204	209	PF02991	0.445
LIG_LIR_Nem_3	22	27	PF02991	0.568
LIG_LIR_Nem_3	279	284	PF02991	0.467
LIG_LIR_Nem_3	316	320	PF02991	0.457
LIG_LIR_Nem_3	337	342	PF02991	0.411
LIG_LIR_Nem_3	343	347	PF02991	0.624
LIG_LIR_Nem_3	36	41	PF02991	0.507
LIG_MYND_1	248	252	PF01753	0.671
LIG_PDZ_Class_1	347	352	PF00595	0.612
LIG_Pex14_2	150	154	PF04695	0.499
LIG_Pex14_2	61	65	PF04695	0.474
LIG_SH2_CRK	206	210	PF00017	0.426
LIG_SH2_SRC	118	121	PF00017	0.523
LIG_SH2_STAT5	120	123	PF00017	0.532
LIG_SH2_STAT5	180	183	PF00017	0.526
LIG_SH2_STAT5	332	335	PF00017	0.468
LIG_SH3_1	242	248	PF00018	0.657
LIG_SH3_1	85	91	PF00018	0.482
LIG_SH3_2	88	93	PF14604	0.463
LIG_SH3_3	222	228	PF00018	0.579
LIG_SH3_3	242	248	PF00018	0.698
LIG_SH3_3	85	91	PF00018	0.482
LIG_SUMO_SIM_anti_2	302	310	PF11976	0.320
LIG_SUMO_SIM_par_1	282	287	PF11976	0.460
LIG_SUMO_SIM_par_1	332	337	PF11976	0.372
LIG_WRC_WIRS_1	159	164	PF05994	0.463
MOD_CDK_SPxK_1	44	50	PF00069	0.597
MOD_CK1_1	167	173	PF00069	0.525
MOD_CK1_1	251	257	PF00069	0.627
MOD_CK1_1	33	39	PF00069	0.530
MOD_CK2_1	22	28	PF00069	0.560
MOD_CK2_1	287	293	PF00069	0.385
MOD_CK2_1	3	9	PF00069	0.627
MOD_CMANNOS	86	89	PF00535	0.274
MOD_GlcNHglycan	131	134	PF01048	0.341
MOD_GlcNHglycan	166	169	PF01048	0.336
MOD_GlcNHglycan	219	223	PF01048	0.461
MOD_GlcNHglycan	289	292	PF01048	0.629
MOD_GlcNHglycan	32	35	PF01048	0.391
MOD_GlcNHglycan	321	324	PF01048	0.691
MOD_GSK3_1	129	136	PF00069	0.544
MOD_GSK3_1	158	165	PF00069	0.535
MOD_GSK3_1	167	174	PF00069	0.486
MOD_GSK3_1	193	200	PF00069	0.551
MOD_GSK3_1	22	29	PF00069	0.539
MOD_GSK3_1	276	283	PF00069	0.507
MOD_NEK2_1	104	109	PF00069	0.474
MOD_NEK2_1	129	134	PF00069	0.554
MOD_NEK2_1	162	167	PF00069	0.382
MOD_NEK2_1	183	188	PF00069	0.526
MOD_NEK2_1	284	289	PF00069	0.422
MOD_NEK2_1	3	8	PF00069	0.617
MOD_NEK2_1	334	339	PF00069	0.356
MOD_NEK2_2	171	176	PF00069	0.512
MOD_NEK2_2	340	345	PF00069	0.308
MOD_PIKK_1	140	146	PF00454	0.499
MOD_PIKK_1	160	166	PF00454	0.500
MOD_PIKK_1	22	28	PF00454	0.619
MOD_PKA_1	79	85	PF00069	0.455
MOD_PKA_2	287	293	PF00069	0.466
MOD_PKA_2	300	306	PF00069	0.522
MOD_PKA_2	89	95	PF00069	0.535
MOD_Plk_1	171	177	PF00069	0.505
MOD_Plk_1	36	42	PF00069	0.550
MOD_Plk_4	105	111	PF00069	0.458
MOD_Plk_4	183	189	PF00069	0.463
MOD_Plk_4	208	214	PF00069	0.439
MOD_Plk_4	251	257	PF00069	0.666
MOD_Plk_4	276	282	PF00069	0.485
MOD_ProDKin_1	241	247	PF00069	0.679
MOD_ProDKin_1	248	254	PF00069	0.595
MOD_ProDKin_1	44	50	PF00069	0.622
MOD_SUMO_rev_2	73	82	PF00179	0.499
MOD_SUMO_rev_2	8	16	PF00179	0.580
TRG_DiLeu_BaLyEn_6	245	250	PF01217	0.607
TRG_ENDOCYTIC_2	118	121	PF00928	0.521
TRG_ENDOCYTIC_2	126	129	PF00928	0.461
TRG_ENDOCYTIC_2	180	183	PF00928	0.510
TRG_ENDOCYTIC_2	206	209	PF00928	0.444
TRG_ENDOCYTIC_2	332	335	PF00928	0.434
TRG_ER_diArg_1	258	260	PF00400	0.614
TRG_NES_CRM1_1	304	316	PF08389	0.432

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4JTD3	Bodo saltans	26%	82%
A0A1X0NTC3	Trypanosomatidae	54%	99%
A0A3Q8IAW1	Leishmania donovani	93%	100%
A0A3R7RDH3	Trypanosoma rangeli	47%	100%
A0A422NZS9	Trypanosoma rangeli	25%	92%
A4H910	Leishmania braziliensis	80%	100%
A4HXE2	Leishmania infantum	93%	100%
Q4QEC6	Leishmania major	90%	100%
V5BE10	Trypanosoma cruzi	45%	99%