Putative tubulin tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin tyrosine ligase, putative

Species:

Leishmania mexicana

UniProt:

E9AQJ5_LEIMU

TriTrypDb:

LmxM.16.0380

Length:

818

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	7
GO:0005929	cilium	4	8
GO:0042995	cell projection	2	8
GO:0043226	organelle	2	8
GO:0043227	membrane-bounded organelle	3	8
GO:0099080	supramolecular complex	2	7
GO:0099081	supramolecular polymer	3	7
GO:0099512	supramolecular fiber	4	7
GO:0099513	polymeric cytoskeletal fiber	5	7
GO:0110165	cellular anatomical entity	1	8
GO:0120025	plasma membrane bounded cell projection	3	8
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

E9AQJ5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AQJ5

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0018095	protein polyglutamylation	7	8
GO:0018193	peptidyl-amino acid modification	5	8
GO:0018200	peptidyl-glutamic acid modification	6	8
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:0000226	microtubule cytoskeleton organization	3	2
GO:0006996	organelle organization	4	2
GO:0007010	cytoskeleton organization	5	2
GO:0007017	microtubule-based process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0071840	cellular component organization or biogenesis	2	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	7
GO:0003824	catalytic activity	1	11
GO:0005488	binding	1	8
GO:0005524	ATP binding	5	7
GO:0016874	ligase activity	2	11
GO:0017076	purine nucleotide binding	4	7
GO:0030554	adenyl nucleotide binding	5	7
GO:0032553	ribonucleotide binding	3	7
GO:0032555	purine ribonucleotide binding	4	7
GO:0032559	adenyl ribonucleotide binding	5	7
GO:0035639	purine ribonucleoside triphosphate binding	4	7
GO:0036094	small molecule binding	2	7
GO:0043167	ion binding	2	7
GO:0043168	anion binding	3	7
GO:0097159	organic cyclic compound binding	2	7
GO:0097367	carbohydrate derivative binding	2	7
GO:1901265	nucleoside phosphate binding	3	7
GO:1901363	heterocyclic compound binding	2	7
GO:0004835	tubulin-tyrosine ligase activity	3	7
GO:0005515	protein binding	2	2
GO:0008092	cytoskeletal protein binding	3	2
GO:0015631	tubulin binding	4	2
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	7
GO:0016881	acid-amino acid ligase activity	4	7
GO:0070739	protein-glutamic acid ligase activity	3	2
GO:0070740	tubulin-glutamic acid ligase activity	4	2
GO:0140096	catalytic activity, acting on a protein	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	100	102	PF00675	0.744
CLV_NRD_NRD_1	192	194	PF00675	0.518
CLV_NRD_NRD_1	221	223	PF00675	0.616
CLV_NRD_NRD_1	331	333	PF00675	0.618
CLV_NRD_NRD_1	364	366	PF00675	0.431
CLV_NRD_NRD_1	393	395	PF00675	0.282
CLV_NRD_NRD_1	403	405	PF00675	0.296
CLV_NRD_NRD_1	451	453	PF00675	0.230
CLV_NRD_NRD_1	486	488	PF00675	0.288
CLV_NRD_NRD_1	71	73	PF00675	0.741
CLV_PCSK_FUR_1	329	333	PF00082	0.565
CLV_PCSK_KEX2_1	100	102	PF00082	0.611
CLV_PCSK_KEX2_1	192	194	PF00082	0.597
CLV_PCSK_KEX2_1	221	223	PF00082	0.616
CLV_PCSK_KEX2_1	331	333	PF00082	0.592
CLV_PCSK_KEX2_1	364	366	PF00082	0.445
CLV_PCSK_KEX2_1	403	405	PF00082	0.294
CLV_PCSK_KEX2_1	451	453	PF00082	0.230
CLV_PCSK_KEX2_1	485	487	PF00082	0.288
CLV_PCSK_SKI1_1	14	18	PF00082	0.624
CLV_PCSK_SKI1_1	502	506	PF00082	0.260
CLV_PCSK_SKI1_1	550	554	PF00082	0.243
CLV_PCSK_SKI1_1	58	62	PF00082	0.629
CLV_PCSK_SKI1_1	768	772	PF00082	0.260
CLV_PCSK_SKI1_1	780	784	PF00082	0.260
DEG_Nend_UBRbox_2	1	3	PF02207	0.637
DEG_SCF_FBW7_1	83	89	PF00400	0.662
DEG_SPOP_SBC_1	321	325	PF00917	0.491
DOC_CKS1_1	83	88	PF01111	0.662
DOC_CYCLIN_RxL_1	448	459	PF00134	0.401
DOC_CYCLIN_yCln2_LP_2	93	99	PF00134	0.534
DOC_MAPK_gen_1	192	200	PF00069	0.605
DOC_MAPK_gen_1	451	457	PF00069	0.433
DOC_MAPK_gen_1	620	628	PF00069	0.492
DOC_MAPK_MEF2A_6	620	628	PF00069	0.492
DOC_MAPK_NFAT4_5	621	629	PF00069	0.492
DOC_MAPK_RevD_3	505	521	PF00069	0.401
DOC_PP1_RVXF_1	708	715	PF00149	0.482
DOC_PP1_RVXF_1	778	784	PF00149	0.460
DOC_USP7_MATH_1	161	165	PF00917	0.641
DOC_USP7_MATH_1	185	189	PF00917	0.698
DOC_USP7_MATH_1	212	216	PF00917	0.593
DOC_USP7_MATH_1	225	229	PF00917	0.643
DOC_USP7_MATH_1	27	31	PF00917	0.584
DOC_USP7_MATH_1	270	274	PF00917	0.710
DOC_USP7_MATH_1	321	325	PF00917	0.607
DOC_USP7_MATH_1	41	45	PF00917	0.582
DOC_USP7_MATH_1	426	430	PF00917	0.484
DOC_USP7_MATH_1	438	442	PF00917	0.484
DOC_USP7_MATH_1	585	589	PF00917	0.584
DOC_USP7_MATH_1	592	596	PF00917	0.510
DOC_USP7_MATH_1	654	658	PF00917	0.534
DOC_USP7_MATH_1	756	760	PF00917	0.467
DOC_USP7_UBL2_3	59	63	PF12436	0.558
DOC_USP7_UBL2_3	8	12	PF12436	0.578
DOC_WW_Pin1_4	120	125	PF00397	0.548
DOC_WW_Pin1_4	21	26	PF00397	0.627
DOC_WW_Pin1_4	275	280	PF00397	0.628
DOC_WW_Pin1_4	44	49	PF00397	0.620
DOC_WW_Pin1_4	530	535	PF00397	0.465
DOC_WW_Pin1_4	571	576	PF00397	0.492
DOC_WW_Pin1_4	754	759	PF00397	0.460
DOC_WW_Pin1_4	82	87	PF00397	0.663
DOC_WW_Pin1_4	92	97	PF00397	0.583
LIG_14-3-3_CanoR_1	246	255	PF00244	0.565
LIG_14-3-3_CanoR_1	331	336	PF00244	0.558
LIG_14-3-3_CanoR_1	365	371	PF00244	0.354
LIG_14-3-3_CanoR_1	416	420	PF00244	0.460
LIG_14-3-3_CanoR_1	452	458	PF00244	0.484
LIG_14-3-3_CanoR_1	537	541	PF00244	0.430
LIG_14-3-3_CanoR_1	81	86	PF00244	0.681
LIG_Actin_WH2_2	705	723	PF00022	0.476
LIG_APCC_ABBA_1	476	481	PF00400	0.460
LIG_BIR_III_4	170	174	PF00653	0.624
LIG_BRCT_BRCA1_1	371	375	PF00533	0.445
LIG_BRCT_BRCA1_1	440	444	PF00533	0.459
LIG_BRCT_BRCA1_1	458	462	PF00533	0.488
LIG_BRCT_BRCA1_1	576	580	PF00533	0.488
LIG_deltaCOP1_diTrp_1	355	360	PF00928	0.382
LIG_eIF4E_1	610	616	PF01652	0.460
LIG_eIF4E_1	737	743	PF01652	0.460
LIG_FHA_1	193	199	PF00498	0.613
LIG_FHA_1	269	275	PF00498	0.513
LIG_FHA_1	281	287	PF00498	0.478
LIG_FHA_1	323	329	PF00498	0.538
LIG_FHA_1	467	473	PF00498	0.478
LIG_FHA_1	487	493	PF00498	0.463
LIG_FHA_1	525	531	PF00498	0.420
LIG_FHA_1	760	766	PF00498	0.589
LIG_FHA_1	776	782	PF00498	0.404
LIG_FHA_1	87	93	PF00498	0.764
LIG_FHA_2	247	253	PF00498	0.568
LIG_FHA_2	391	397	PF00498	0.460
LIG_FHA_2	419	425	PF00498	0.461
LIG_FHA_2	762	768	PF00498	0.460
LIG_Integrin_isoDGR_2	219	221	PF01839	0.585
LIG_LIR_Apic_2	469	473	PF02991	0.491
LIG_LIR_Apic_2	539	543	PF02991	0.430
LIG_LIR_Gen_1	355	363	PF02991	0.393
LIG_LIR_Gen_1	412	422	PF02991	0.488
LIG_LIR_Gen_1	642	653	PF02991	0.460
LIG_LIR_Gen_1	688	696	PF02991	0.447
LIG_LIR_Nem_3	355	359	PF02991	0.397
LIG_LIR_Nem_3	396	402	PF02991	0.462
LIG_LIR_Nem_3	412	417	PF02991	0.460
LIG_LIR_Nem_3	631	636	PF02991	0.492
LIG_LIR_Nem_3	642	648	PF02991	0.492
LIG_LIR_Nem_3	688	692	PF02991	0.447
LIG_LIR_Nem_3	699	703	PF02991	0.447
LIG_LIR_Nem_3	713	717	PF02991	0.402
LIG_LIR_Nem_3	733	737	PF02991	0.554
LIG_LIR_Nem_3	811	816	PF02991	0.401
LIG_MAD2	780	788	PF02301	0.460
LIG_MLH1_MIPbox_1	371	375	PF16413	0.362
LIG_PCNA_PIPBox_1	660	669	PF02747	0.460
LIG_PDZ_Class_2	813	818	PF00595	0.383
LIG_Pex14_2	479	483	PF04695	0.488
LIG_Pex14_2	689	693	PF04695	0.447
LIG_PTB_Apo_2	708	715	PF02174	0.460
LIG_REV1ctd_RIR_1	372	380	PF16727	0.350
LIG_SH2_CRK	399	403	PF00017	0.463
LIG_SH2_CRK	414	418	PF00017	0.460
LIG_SH2_CRK	470	474	PF00017	0.460
LIG_SH2_CRK	633	637	PF00017	0.492
LIG_SH2_CRK	717	721	PF00017	0.460
LIG_SH2_CRK	739	743	PF00017	0.460
LIG_SH2_GRB2like	341	344	PF00017	0.479
LIG_SH2_NCK_1	470	474	PF00017	0.460
LIG_SH2_PTP2	627	630	PF00017	0.460
LIG_SH2_SRC	605	608	PF00017	0.465
LIG_SH2_STAP1	739	743	PF00017	0.460
LIG_SH2_STAT5	405	408	PF00017	0.460
LIG_SH2_STAT5	605	608	PF00017	0.492
LIG_SH2_STAT5	610	613	PF00017	0.492
LIG_SH2_STAT5	627	630	PF00017	0.492
LIG_SH2_STAT5	639	642	PF00017	0.492
LIG_SH2_STAT5	695	698	PF00017	0.492
LIG_SH2_STAT5	703	706	PF00017	0.492
LIG_SH3_3	112	118	PF00018	0.533
LIG_SH3_3	132	138	PF00018	0.572
LIG_SH3_3	179	185	PF00018	0.757
LIG_SH3_3	273	279	PF00018	0.545
LIG_SH3_3	572	578	PF00018	0.458
LIG_SH3_3	779	785	PF00018	0.460
LIG_SH3_3	85	91	PF00018	0.645
LIG_SUMO_SIM_anti_2	195	201	PF11976	0.608
LIG_SUMO_SIM_par_1	453	459	PF11976	0.465
LIG_TRAF2_1	334	337	PF00917	0.450
LIG_TRAF2_2	139	144	PF00917	0.528
LIG_TYR_ITAM	396	417	PF00017	0.460
LIG_TYR_ITIM	397	402	PF00017	0.460
LIG_TYR_ITIM	715	720	PF00017	0.460
LIG_WRC_WIRS_1	457	462	PF05994	0.488
LIG_WRC_WIRS_1	526	531	PF05994	0.488
LIG_WW_1	784	787	PF00397	0.488
LIG_WW_3	137	141	PF00397	0.521
MOD_CDK_SPK_2	574	579	PF00069	0.436
MOD_CDK_SPxxK_3	120	127	PF00069	0.532
MOD_CDK_SPxxK_3	530	537	PF00069	0.465
MOD_CK1_1	120	126	PF00069	0.592
MOD_CK1_1	230	236	PF00069	0.759
MOD_CK1_1	278	284	PF00069	0.613
MOD_CK1_1	30	36	PF00069	0.558
MOD_CK1_1	44	50	PF00069	0.605
MOD_CK1_1	456	462	PF00069	0.465
MOD_CK1_1	533	539	PF00069	0.420
MOD_CK1_1	556	562	PF00069	0.515
MOD_CK1_1	571	577	PF00069	0.475
MOD_CK1_1	584	590	PF00069	0.445
MOD_CK1_1	665	671	PF00069	0.445
MOD_CK1_1	759	765	PF00069	0.478
MOD_CK1_1	84	90	PF00069	0.630
MOD_CK1_1	95	101	PF00069	0.673
MOD_CK2_1	331	337	PF00069	0.461
MOD_CK2_1	339	345	PF00069	0.467
MOD_CK2_1	552	558	PF00069	0.526
MOD_Cter_Amidation	190	193	PF01082	0.585
MOD_Cter_Amidation	219	222	PF01082	0.585
MOD_Cter_Amidation	618	621	PF01082	0.292
MOD_GlcNHglycan	105	108	PF01048	0.622
MOD_GlcNHglycan	178	181	PF01048	0.679
MOD_GlcNHglycan	207	210	PF01048	0.629
MOD_GlcNHglycan	214	217	PF01048	0.663
MOD_GlcNHglycan	234	237	PF01048	0.694
MOD_GlcNHglycan	280	283	PF01048	0.621
MOD_GlcNHglycan	291	294	PF01048	0.689
MOD_GlcNHglycan	304	307	PF01048	0.653
MOD_GlcNHglycan	426	429	PF01048	0.294
MOD_GlcNHglycan	43	46	PF01048	0.683
MOD_GlcNHglycan	558	561	PF01048	0.341
MOD_GlcNHglycan	564	567	PF01048	0.273
MOD_GlcNHglycan	583	586	PF01048	0.286
MOD_GlcNHglycan	594	597	PF01048	0.260
MOD_GlcNHglycan	8	11	PF01048	0.774
MOD_GlcNHglycan	97	100	PF01048	0.712
MOD_GSK3_1	161	168	PF00069	0.719
MOD_GSK3_1	2	9	PF00069	0.621
MOD_GSK3_1	200	207	PF00069	0.553
MOD_GSK3_1	227	234	PF00069	0.723
MOD_GSK3_1	265	272	PF00069	0.594
MOD_GSK3_1	298	305	PF00069	0.646
MOD_GSK3_1	420	427	PF00069	0.486
MOD_GSK3_1	462	469	PF00069	0.474
MOD_GSK3_1	520	527	PF00069	0.465
MOD_GSK3_1	529	536	PF00069	0.460
MOD_GSK3_1	552	559	PF00069	0.500
MOD_GSK3_1	581	588	PF00069	0.511
MOD_GSK3_1	601	608	PF00069	0.448
MOD_GSK3_1	71	78	PF00069	0.613
MOD_GSK3_1	754	761	PF00069	0.460
MOD_GSK3_1	800	807	PF00069	0.399
MOD_GSK3_1	82	89	PF00069	0.698
MOD_N-GLC_1	204	209	PF02516	0.608
MOD_N-GLC_1	524	529	PF02516	0.221
MOD_N-GLC_1	679	684	PF02516	0.263
MOD_N-GLC_1	754	759	PF02516	0.260
MOD_N-GLC_2	568	570	PF02516	0.265
MOD_NEK2_1	280	285	PF00069	0.579
MOD_NEK2_1	462	467	PF00069	0.460
MOD_NEK2_1	529	534	PF00069	0.461
MOD_NEK2_1	601	606	PF00069	0.507
MOD_NEK2_1	629	634	PF00069	0.460
MOD_NEK2_1	648	653	PF00069	0.460
MOD_NEK2_1	760	765	PF00069	0.460
MOD_NEK2_1	775	780	PF00069	0.460
MOD_NEK2_2	227	232	PF00069	0.550
MOD_NEK2_2	241	246	PF00069	0.619
MOD_NEK2_2	415	420	PF00069	0.460
MOD_NEK2_2	439	444	PF00069	0.401
MOD_PIKK_1	323	329	PF00454	0.589
MOD_PIKK_1	486	492	PF00454	0.460
MOD_PIKK_1	496	502	PF00454	0.460
MOD_PIKK_1	568	574	PF00454	0.411
MOD_PIKK_1	585	591	PF00454	0.465
MOD_PK_1	331	337	PF00069	0.461
MOD_PKA_1	100	106	PF00069	0.648
MOD_PKA_1	192	198	PF00069	0.598
MOD_PKA_1	331	337	PF00069	0.461
MOD_PKA_1	486	492	PF00069	0.488
MOD_PKA_1	520	526	PF00069	0.447
MOD_PKA_1	58	64	PF00069	0.615
MOD_PKA_2	100	106	PF00069	0.648
MOD_PKA_2	192	198	PF00069	0.617
MOD_PKA_2	302	308	PF00069	0.674
MOD_PKA_2	331	337	PF00069	0.556
MOD_PKA_2	366	372	PF00069	0.408
MOD_PKA_2	415	421	PF00069	0.461
MOD_PKA_2	486	492	PF00069	0.460
MOD_PKA_2	536	542	PF00069	0.425
MOD_PKA_2	71	77	PF00069	0.814
MOD_PKB_1	329	337	PF00069	0.468
MOD_PKB_1	79	87	PF00069	0.625
MOD_Plk_1	241	247	PF00069	0.649
MOD_Plk_1	474	480	PF00069	0.440
MOD_Plk_1	524	530	PF00069	0.410
MOD_Plk_2-3	730	736	PF00069	0.489
MOD_Plk_4	439	445	PF00069	0.456
MOD_Plk_4	474	480	PF00069	0.465
MOD_Plk_4	525	531	PF00069	0.488
MOD_Plk_4	536	542	PF00069	0.434
MOD_Plk_4	601	607	PF00069	0.491
MOD_ProDKin_1	120	126	PF00069	0.543
MOD_ProDKin_1	21	27	PF00069	0.629
MOD_ProDKin_1	275	281	PF00069	0.627
MOD_ProDKin_1	44	50	PF00069	0.621
MOD_ProDKin_1	530	536	PF00069	0.465
MOD_ProDKin_1	571	577	PF00069	0.492
MOD_ProDKin_1	754	760	PF00069	0.460
MOD_ProDKin_1	82	88	PF00069	0.661
MOD_ProDKin_1	92	98	PF00069	0.582
MOD_SUMO_rev_2	342	351	PF00179	0.512
TRG_AP2beta_CARGO_1	642	652	PF09066	0.460
TRG_DiLeu_BaLyEn_6	611	616	PF01217	0.460
TRG_DiLeu_BaLyEn_6	765	770	PF01217	0.460
TRG_DiLeu_BaLyEn_6	811	816	PF01217	0.390
TRG_DiLeu_BaLyEn_6	88	93	PF01217	0.576
TRG_ENDOCYTIC_2	399	402	PF00928	0.463
TRG_ENDOCYTIC_2	414	417	PF00928	0.460
TRG_ENDOCYTIC_2	627	630	PF00928	0.484
TRG_ENDOCYTIC_2	633	636	PF00928	0.503
TRG_ENDOCYTIC_2	717	720	PF00928	0.460
TRG_ENDOCYTIC_2	739	742	PF00928	0.460
TRG_ER_diArg_1	221	223	PF00400	0.568
TRG_ER_diArg_1	328	331	PF00400	0.627
TRG_ER_diArg_1	363	365	PF00400	0.506
TRG_ER_diArg_1	402	404	PF00400	0.511
TRG_ER_diArg_1	451	453	PF00400	0.453
TRG_ER_diArg_1	485	487	PF00400	0.488
TRG_ER_diArg_1	799	802	PF00400	0.376
TRG_NES_CRM1_1	661	676	PF08389	0.460
TRG_NLS_MonoExtC_3	100	105	PF00514	0.568
TRG_NLS_MonoExtN_4	100	105	PF00514	0.588
TRG_Pf-PMV_PEXEL_1	157	162	PF00026	0.661
TRG_Pf-PMV_PEXEL_1	404	408	PF00026	0.260
TRG_Pf-PMV_PEXEL_1	515	519	PF00026	0.201
TRG_Pf-PMV_PEXEL_1	768	772	PF00026	0.260

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDX5	Leptomonas seymouri	60%	100%
A0A3Q8IDL7	Leishmania donovani	25%	100%
A0A3S5H6X0	Leishmania donovani	86%	100%
A4H8G3	Leishmania braziliensis	69%	94%
A4HWT7	Leishmania infantum	86%	100%
A4HYN9	Leishmania infantum	24%	100%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4QCW7	Leishmania major	25%	100%
Q4QEY2	Leishmania major	84%	99%