tRNA (guanine(37)-N1)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Met-10+ like-protein, putative

Species:

Leishmania mexicana

UniProt:

E9AQI8_LEIMU

TriTrypDb:

LmxM.16.0310

Length:

688

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	7
GO:0005759	mitochondrial matrix	5	7
GO:0031974	membrane-enclosed lumen	2	7
GO:0043226	organelle	2	7
GO:0043227	membrane-bounded organelle	3	7
GO:0043229	intracellular organelle	3	7
GO:0043231	intracellular membrane-bounded organelle	4	7
GO:0043233	organelle lumen	3	7
GO:0070013	intracellular organelle lumen	4	7
GO:0110165	cellular anatomical entity	1	7
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

E9AQI8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AQI8

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0002939	tRNA N1-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0008168	methyltransferase activity	4	7
GO:0008173	RNA methyltransferase activity	4	7
GO:0008175	tRNA methyltransferase activity	5	7
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	7
GO:0009019	tRNA (guanine-N1-)-methyltransferase activity	7	7
GO:0016423	tRNA (guanine) methyltransferase activity	6	7
GO:0016740	transferase activity	2	7
GO:0016741	transferase activity, transferring one-carbon groups	3	7
GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity	8	7
GO:0140098	catalytic activity, acting on RNA	3	7
GO:0140101	catalytic activity, acting on a tRNA	4	7
GO:0140640	catalytic activity, acting on a nucleic acid	2	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	108	112	PF00656	0.493
CLV_C14_Caspase3-7	350	354	PF00656	0.670
CLV_NRD_NRD_1	190	192	PF00675	0.608
CLV_NRD_NRD_1	240	242	PF00675	0.745
CLV_NRD_NRD_1	347	349	PF00675	0.575
CLV_NRD_NRD_1	524	526	PF00675	0.297
CLV_NRD_NRD_1	54	56	PF00675	0.607
CLV_NRD_NRD_1	583	585	PF00675	0.376
CLV_PCSK_KEX2_1	239	241	PF00082	0.670
CLV_PCSK_KEX2_1	346	348	PF00082	0.528
CLV_PCSK_KEX2_1	524	526	PF00082	0.306
CLV_PCSK_KEX2_1	53	55	PF00082	0.590
CLV_PCSK_KEX2_1	555	557	PF00082	0.462
CLV_PCSK_KEX2_1	583	585	PF00082	0.384
CLV_PCSK_KEX2_1	632	634	PF00082	0.454
CLV_PCSK_KEX2_1	681	683	PF00082	0.621
CLV_PCSK_PC1ET2_1	239	241	PF00082	0.670
CLV_PCSK_PC1ET2_1	555	557	PF00082	0.462
CLV_PCSK_PC1ET2_1	632	634	PF00082	0.454
CLV_PCSK_PC1ET2_1	681	683	PF00082	0.621
CLV_PCSK_PC7_1	520	526	PF00082	0.186
CLV_PCSK_SKI1_1	336	340	PF00082	0.279
CLV_PCSK_SKI1_1	35	39	PF00082	0.544
CLV_PCSK_SKI1_1	398	402	PF00082	0.447
CLV_PCSK_SKI1_1	497	501	PF00082	0.394
CLV_PCSK_SKI1_1	525	529	PF00082	0.321
CLV_PCSK_SKI1_1	58	62	PF00082	0.592
CLV_PCSK_SKI1_1	584	588	PF00082	0.295
CLV_PCSK_SKI1_1	681	685	PF00082	0.680
DEG_APCC_DBOX_1	496	504	PF00400	0.594
DEG_APCC_DBOX_1	582	590	PF00400	0.402
DEG_SPOP_SBC_1	544	548	PF00917	0.603
DOC_MAPK_gen_1	20	29	PF00069	0.619
DOC_MAPK_gen_1	336	345	PF00069	0.539
DOC_MAPK_gen_1	434	442	PF00069	0.455
DOC_MAPK_gen_1	524	531	PF00069	0.534
DOC_MAPK_gen_1	632	639	PF00069	0.430
DOC_MAPK_MEF2A_6	632	639	PF00069	0.430
DOC_MAPK_RevD_3	42	55	PF00069	0.558
DOC_PP1_RVXF_1	415	421	PF00149	0.521
DOC_PP1_SILK_1	44	49	PF00149	0.604
DOC_PP2B_PxIxI_1	531	537	PF00149	0.506
DOC_PP4_FxxP_1	411	414	PF00568	0.521
DOC_PP4_FxxP_1	451	454	PF00568	0.521
DOC_PP4_FxxP_1	542	545	PF00568	0.276
DOC_PP4_FxxP_1	639	642	PF00568	0.413
DOC_USP7_MATH_1	116	120	PF00917	0.661
DOC_USP7_MATH_1	3	7	PF00917	0.735
DOC_USP7_MATH_1	330	334	PF00917	0.521
DOC_USP7_MATH_1	368	372	PF00917	0.595
DOC_USP7_MATH_1	485	489	PF00917	0.545
DOC_USP7_MATH_1	512	516	PF00917	0.572
DOC_USP7_MATH_1	65	69	PF00917	0.645
DOC_WW_Pin1_4	121	126	PF00397	0.782
DOC_WW_Pin1_4	159	164	PF00397	0.528
DOC_WW_Pin1_4	383	388	PF00397	0.510
DOC_WW_Pin1_4	666	671	PF00397	0.515
LIG_14-3-3_CanoR_1	240	245	PF00244	0.573
LIG_14-3-3_CanoR_1	35	44	PF00244	0.384
LIG_14-3-3_CanoR_1	423	427	PF00244	0.521
LIG_14-3-3_CanoR_1	524	530	PF00244	0.521
LIG_14-3-3_CanoR_1	53	58	PF00244	0.441
LIG_14-3-3_CanoR_1	556	565	PF00244	0.505
LIG_14-3-3_CanoR_1	583	587	PF00244	0.415
LIG_Actin_WH2_2	133	148	PF00022	0.525
LIG_AP2alpha_1	538	542	PF02296	0.594
LIG_BIR_II_1	1	5	PF00653	0.780
LIG_BRCT_BRCA1_1	331	335	PF00533	0.521
LIG_BRCT_BRCA1_1	501	505	PF00533	0.594
LIG_DLG_GKlike_1	240	248	PF00625	0.583
LIG_EH1_1	263	271	PF00400	0.571
LIG_eIF4E_1	264	270	PF01652	0.463
LIG_FHA_1	175	181	PF00498	0.537
LIG_FHA_1	24	30	PF00498	0.503
LIG_FHA_1	243	249	PF00498	0.540
LIG_FHA_1	318	324	PF00498	0.516
LIG_FHA_1	347	353	PF00498	0.645
LIG_FHA_1	386	392	PF00498	0.535
LIG_FHA_1	399	405	PF00498	0.238
LIG_FHA_1	427	433	PF00498	0.506
LIG_FHA_1	526	532	PF00498	0.521
LIG_FHA_1	559	565	PF00498	0.415
LIG_FHA_1	620	626	PF00498	0.511
LIG_FHA_2	212	218	PF00498	0.523
LIG_FHA_2	263	269	PF00498	0.471
LIG_FHA_2	371	377	PF00498	0.429
LIG_LIR_Apic_2	148	154	PF02991	0.569
LIG_LIR_Apic_2	410	414	PF02991	0.539
LIG_LIR_Apic_2	540	545	PF02991	0.434
LIG_LIR_Apic_2	638	642	PF02991	0.413
LIG_LIR_Apic_2	84	88	PF02991	0.764
LIG_LIR_Gen_1	286	295	PF02991	0.387
LIG_LIR_Gen_1	439	450	PF02991	0.521
LIG_LIR_Gen_1	502	513	PF02991	0.511
LIG_LIR_Gen_1	537	545	PF02991	0.521
LIG_LIR_Nem_3	258	264	PF02991	0.485
LIG_LIR_Nem_3	286	291	PF02991	0.335
LIG_LIR_Nem_3	332	338	PF02991	0.521
LIG_LIR_Nem_3	502	508	PF02991	0.497
LIG_LIR_Nem_3	537	541	PF02991	0.521
LIG_LIR_Nem_3	585	590	PF02991	0.243
LIG_PDZ_Class_3	683	688	PF00595	0.477
LIG_Pex14_2	538	542	PF04695	0.521
LIG_SH2_CRK	34	38	PF00017	0.507
LIG_SH2_CRK	415	419	PF00017	0.521
LIG_SH2_CRK	474	478	PF00017	0.521
LIG_SH2_CRK	612	616	PF00017	0.484
LIG_SH2_GRB2like	419	422	PF00017	0.521
LIG_SH2_NCK_1	389	393	PF00017	0.528
LIG_SH2_NCK_1	415	419	PF00017	0.521
LIG_SH2_NCK_1	549	553	PF00017	0.632
LIG_SH2_NCK_1	612	616	PF00017	0.484
LIG_SH2_SRC	306	309	PF00017	0.594
LIG_SH2_SRC	549	552	PF00017	0.465
LIG_SH2_STAP1	306	310	PF00017	0.521
LIG_SH2_STAT5	244	247	PF00017	0.617
LIG_SH2_STAT5	264	267	PF00017	0.473
LIG_SH2_STAT5	590	593	PF00017	0.328
LIG_SH2_STAT5	612	615	PF00017	0.575
LIG_SH2_STAT5	85	88	PF00017	0.784
LIG_SH3_3	122	128	PF00018	0.772
LIG_SH3_3	230	236	PF00018	0.789
LIG_SH3_3	30	36	PF00018	0.457
LIG_SH3_3	538	544	PF00018	0.521
LIG_SUMO_SIM_anti_2	319	325	PF11976	0.521
LIG_SUMO_SIM_par_1	281	286	PF11976	0.515
LIG_SUMO_SIM_par_1	378	383	PF11976	0.369
LIG_SUMO_SIM_par_1	62	68	PF11976	0.682
LIG_SUMO_SIM_par_1	620	627	PF11976	0.444
LIG_TRAF2_1	183	186	PF00917	0.332
LIG_TRAF2_1	654	657	PF00917	0.424
LIG_TRFH_1	612	616	PF08558	0.484
LIG_TRFH_1	639	643	PF08558	0.526
LIG_TYR_ITIM	304	309	PF00017	0.387
LIG_TYR_ITIM	32	37	PF00017	0.464
LIG_TYR_ITIM	413	418	PF00017	0.387
LIG_UBA3_1	43	50	PF00899	0.604
MOD_CK1_1	112	118	PF00069	0.721
MOD_CK1_1	119	125	PF00069	0.611
MOD_CK1_1	174	180	PF00069	0.517
MOD_CK1_1	209	215	PF00069	0.790
MOD_CK1_1	422	428	PF00069	0.387
MOD_CK1_1	6	12	PF00069	0.721
MOD_CK1_1	76	82	PF00069	0.783
MOD_CK1_1	99	105	PF00069	0.646
MOD_CK2_1	15	21	PF00069	0.533
MOD_CK2_1	209	215	PF00069	0.797
MOD_CK2_1	22	28	PF00069	0.387
MOD_CK2_1	262	268	PF00069	0.484
MOD_CK2_1	283	289	PF00069	0.492
MOD_CK2_1	370	376	PF00069	0.448
MOD_CK2_1	387	393	PF00069	0.615
MOD_CK2_1	5	11	PF00069	0.764
MOD_CK2_1	590	596	PF00069	0.439
MOD_Cter_Amidation	522	525	PF01082	0.387
MOD_Cter_Amidation	553	556	PF01082	0.462
MOD_GlcNHglycan	1	4	PF01048	0.778
MOD_GlcNHglycan	101	104	PF01048	0.675
MOD_GlcNHglycan	13	16	PF01048	0.577
MOD_GlcNHglycan	155	158	PF01048	0.593
MOD_GlcNHglycan	285	288	PF01048	0.496
MOD_GlcNHglycan	299	302	PF01048	0.387
MOD_GlcNHglycan	363	367	PF01048	0.612
MOD_GlcNHglycan	39	42	PF01048	0.458
MOD_GlcNHglycan	552	555	PF01048	0.554
MOD_GlcNHglycan	592	595	PF01048	0.439
MOD_GlcNHglycan	75	78	PF01048	0.702
MOD_GSK3_1	112	119	PF00069	0.643
MOD_GSK3_1	207	214	PF00069	0.749
MOD_GSK3_1	264	271	PF00069	0.526
MOD_GSK3_1	383	390	PF00069	0.606
MOD_GSK3_1	422	429	PF00069	0.357
MOD_GSK3_1	5	12	PF00069	0.764
MOD_N-GLC_1	119	124	PF02516	0.761
MOD_N-GLC_1	23	28	PF02516	0.535
MOD_N-GLC_1	297	302	PF02516	0.387
MOD_N-GLC_1	317	322	PF02516	0.387
MOD_N-GLC_1	557	562	PF02516	0.609
MOD_N-GLC_1	99	104	PF02516	0.643
MOD_NEK2_1	37	42	PF00069	0.471
MOD_NEK2_1	400	405	PF00069	0.333
MOD_NEK2_1	426	431	PF00069	0.456
MOD_NEK2_1	499	504	PF00069	0.387
MOD_NEK2_1	610	615	PF00069	0.574
MOD_NEK2_1	635	640	PF00069	0.418
MOD_NEK2_1	649	654	PF00069	0.372
MOD_NEK2_2	330	335	PF00069	0.387
MOD_NEK2_2	485	490	PF00069	0.391
MOD_PK_1	53	59	PF00069	0.595
MOD_PKA_1	240	246	PF00069	0.587
MOD_PKA_1	346	352	PF00069	0.505
MOD_PKA_1	53	59	PF00069	0.595
MOD_PKA_2	109	115	PF00069	0.616
MOD_PKA_2	240	246	PF00069	0.535
MOD_PKA_2	3	9	PF00069	0.680
MOD_PKA_2	346	352	PF00069	0.505
MOD_PKA_2	422	428	PF00069	0.387
MOD_PKA_2	519	525	PF00069	0.197
MOD_PKA_2	53	59	PF00069	0.578
MOD_PKA_2	582	588	PF00069	0.456
MOD_Plk_1	23	29	PF00069	0.541
MOD_Plk_1	248	254	PF00069	0.553
MOD_Plk_1	317	323	PF00069	0.345
MOD_Plk_1	426	432	PF00069	0.346
MOD_Plk_1	619	625	PF00069	0.509
MOD_Plk_4	140	146	PF00069	0.442
MOD_Plk_4	268	274	PF00069	0.461
MOD_Plk_4	319	325	PF00069	0.387
MOD_Plk_4	330	336	PF00069	0.319
MOD_Plk_4	42	48	PF00069	0.490
MOD_Plk_4	499	505	PF00069	0.387
MOD_Plk_4	619	625	PF00069	0.509
MOD_ProDKin_1	121	127	PF00069	0.780
MOD_ProDKin_1	159	165	PF00069	0.513
MOD_ProDKin_1	383	389	PF00069	0.505
MOD_ProDKin_1	666	672	PF00069	0.512
MOD_SUMO_rev_2	332	340	PF00179	0.387
MOD_SUMO_rev_2	76	81	PF00179	0.703
TRG_DiLeu_BaEn_1	278	283	PF01217	0.612
TRG_DiLeu_BaEn_1	28	33	PF01217	0.568
TRG_DiLeu_BaEn_1	427	432	PF01217	0.387
TRG_DiLeu_BaLyEn_6	395	400	PF01217	0.495
TRG_ENDOCYTIC_2	106	109	PF00928	0.490
TRG_ENDOCYTIC_2	306	309	PF00928	0.387
TRG_ENDOCYTIC_2	34	37	PF00928	0.475
TRG_ENDOCYTIC_2	415	418	PF00928	0.387
TRG_ENDOCYTIC_2	474	477	PF00928	0.328
TRG_ER_diArg_1	345	348	PF00400	0.610
TRG_ER_diArg_1	434	437	PF00400	0.294
TRG_ER_diArg_1	52	55	PF00400	0.634
TRG_NES_CRM1_1	329	341	PF08389	0.365
TRG_NLS_MonoExtC_3	680	686	PF00514	0.673
TRG_NLS_MonoExtN_4	236	243	PF00514	0.757

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I219	Leptomonas seymouri	53%	100%
A0A3S7WTS8	Leishmania donovani	90%	100%
A4H8F7	Leishmania braziliensis	74%	100%
A4HWT0	Leishmania infantum	90%	100%
B7G5J1	Phaeodactylum tricornutum (strain CCAP 1055/1)	25%	100%
Q4QEY9	Leishmania major	91%	100%