tRNA_SAD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9AQ67_LEIMU

TriTrypDb:

LmxM.15.0690

Length:

491

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9AQ67

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AQ67

Function

Biological processes
Term	Name	Level	Count
GO:0006450	regulation of translational fidelity	3	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0006082	organic acid metabolic process	3	4
GO:0006139	nucleobase-containing compound metabolic process	3	4
GO:0006399	tRNA metabolic process	7	4
GO:0006520	amino acid metabolic process	3	4
GO:0006725	cellular aromatic compound metabolic process	3	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0008152	metabolic process	1	4
GO:0009987	cellular process	1	4
GO:0016070	RNA metabolic process	5	4
GO:0019752	carboxylic acid metabolic process	5	4
GO:0034641	cellular nitrogen compound metabolic process	3	4
GO:0034660	ncRNA metabolic process	6	4
GO:0043038	amino acid activation	4	4
GO:0043039	tRNA aminoacylation	5	4
GO:0043170	macromolecule metabolic process	3	4
GO:0043436	oxoacid metabolic process	4	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044281	small molecule metabolic process	2	4
GO:0046483	heterocycle metabolic process	3	4
GO:0071704	organic substance metabolic process	2	4
GO:0090304	nucleic acid metabolic process	4	4
GO:1901360	organic cyclic compound metabolic process	3	4
GO:1901564	organonitrogen compound metabolic process	3	4
GO:0006418	tRNA aminoacylation for protein translation	6	3
GO:0006419	alanyl-tRNA aminoacylation	7	3

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0005488	binding	1	11
GO:0036094	small molecule binding	2	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:0002161	aminoacyl-tRNA editing activity	5	1
GO:0002196	Ser-tRNA(Ala) hydrolase activity	6	1
GO:0003824	catalytic activity	1	5
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0052689	carboxylic ester hydrolase activity	4	1
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140101	catalytic activity, acting on a tRNA	4	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5
GO:0004812	aminoacyl-tRNA ligase activity	4	4
GO:0005524	ATP binding	5	4
GO:0016874	ligase activity	2	4
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097367	carbohydrate derivative binding	2	4
GO:0004813	alanine-tRNA ligase activity	5	3
GO:0003676	nucleic acid binding	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	270	274	PF00656	0.455
CLV_MEL_PAP_1	4	10	PF00089	0.457
CLV_NRD_NRD_1	103	105	PF00675	0.417
CLV_NRD_NRD_1	131	133	PF00675	0.392
CLV_NRD_NRD_1	150	152	PF00675	0.395
CLV_NRD_NRD_1	235	237	PF00675	0.586
CLV_NRD_NRD_1	345	347	PF00675	0.420
CLV_NRD_NRD_1	389	391	PF00675	0.406
CLV_NRD_NRD_1	411	413	PF00675	0.475
CLV_PCSK_KEX2_1	103	105	PF00082	0.417
CLV_PCSK_KEX2_1	131	133	PF00082	0.392
CLV_PCSK_KEX2_1	235	237	PF00082	0.595
CLV_PCSK_KEX2_1	345	347	PF00082	0.459
CLV_PCSK_KEX2_1	358	360	PF00082	0.394
CLV_PCSK_KEX2_1	389	391	PF00082	0.406
CLV_PCSK_KEX2_1	411	413	PF00082	0.475
CLV_PCSK_PC1ET2_1	358	360	PF00082	0.473
CLV_PCSK_PC7_1	231	237	PF00082	0.539
CLV_PCSK_SKI1_1	20	24	PF00082	0.434
CLV_PCSK_SKI1_1	348	352	PF00082	0.431
CLV_PCSK_SKI1_1	355	359	PF00082	0.402
CLV_PCSK_SKI1_1	412	416	PF00082	0.407
DEG_APCC_DBOX_1	401	409	PF00400	0.236
DOC_CYCLIN_yCln2_LP_2	111	117	PF00134	0.505
DOC_CYCLIN_yCln2_LP_2	164	170	PF00134	0.437
DOC_MAPK_gen_1	151	157	PF00069	0.347
DOC_MAPK_gen_1	355	364	PF00069	0.439
DOC_MAPK_gen_1	389	397	PF00069	0.458
DOC_MAPK_gen_1	402	410	PF00069	0.459
DOC_MAPK_MEF2A_6	299	307	PF00069	0.385
DOC_MAPK_MEF2A_6	389	397	PF00069	0.491
DOC_PP1_RVXF_1	302	308	PF00149	0.348
DOC_PP1_RVXF_1	468	474	PF00149	0.557
DOC_PP2B_LxvP_1	155	158	PF13499	0.508
DOC_USP7_MATH_1	158	162	PF00917	0.422
DOC_USP7_MATH_1	240	244	PF00917	0.660
DOC_USP7_MATH_1	250	254	PF00917	0.521
DOC_USP7_MATH_1	271	275	PF00917	0.536
DOC_USP7_MATH_1	85	89	PF00917	0.617
DOC_USP7_UBL2_3	476	480	PF12436	0.448
DOC_WW_Pin1_4	110	115	PF00397	0.458
DOC_WW_Pin1_4	181	186	PF00397	0.503
DOC_WW_Pin1_4	443	448	PF00397	0.553
LIG_14-3-3_CanoR_1	265	270	PF00244	0.434
LIG_14-3-3_CanoR_1	359	363	PF00244	0.466
LIG_14-3-3_CanoR_1	371	377	PF00244	0.498
LIG_14-3-3_CanoR_1	423	429	PF00244	0.627
LIG_14-3-3_CanoR_1	43	52	PF00244	0.563
LIG_Actin_WH2_2	398	413	PF00022	0.523
LIG_BIR_II_1	1	5	PF00653	0.475
LIG_BIR_III_4	406	410	PF00653	0.495
LIG_BRCT_BRCA1_1	58	62	PF00533	0.371
LIG_FHA_1	106	112	PF00498	0.422
LIG_FHA_1	144	150	PF00498	0.432
LIG_FHA_1	152	158	PF00498	0.422
LIG_FHA_1	21	27	PF00498	0.424
LIG_FHA_1	259	265	PF00498	0.462
LIG_FHA_1	359	365	PF00498	0.408
LIG_FHA_1	383	389	PF00498	0.522
LIG_FHA_1	430	436	PF00498	0.488
LIG_FHA_1	466	472	PF00498	0.555
LIG_FHA_1	72	78	PF00498	0.522
LIG_FHA_2	200	206	PF00498	0.236
LIG_FHA_2	214	220	PF00498	0.228
LIG_FHA_2	282	288	PF00498	0.428
LIG_FHA_2	349	355	PF00498	0.514
LIG_FHA_2	43	49	PF00498	0.403
LIG_FHA_2	444	450	PF00498	0.535
LIG_LIR_Gen_1	165	175	PF02991	0.404
LIG_LIR_Gen_1	338	343	PF02991	0.477
LIG_LIR_Gen_1	396	405	PF02991	0.390
LIG_LIR_Gen_1	481	489	PF02991	0.507
LIG_LIR_Gen_1	49	60	PF02991	0.366
LIG_LIR_Nem_3	165	171	PF02991	0.417
LIG_LIR_Nem_3	184	189	PF02991	0.360
LIG_LIR_Nem_3	338	342	PF02991	0.506
LIG_LIR_Nem_3	396	401	PF02991	0.371
LIG_LIR_Nem_3	481	485	PF02991	0.512
LIG_LIR_Nem_3	49	55	PF02991	0.385
LIG_LIR_Nem_3	71	75	PF02991	0.375
LIG_PCNA_yPIPBox_3	142	152	PF02747	0.431
LIG_SH2_CRK	168	172	PF00017	0.470
LIG_SH2_CRK	52	56	PF00017	0.440
LIG_SH2_CRK	72	76	PF00017	0.358
LIG_SH2_STAP1	52	56	PF00017	0.440
LIG_SH2_STAT5	168	171	PF00017	0.404
LIG_SH2_STAT5	189	192	PF00017	0.490
LIG_SH2_STAT5	292	295	PF00017	0.476
LIG_SH2_STAT5	306	309	PF00017	0.324
LIG_SH3_3	124	130	PF00018	0.453
LIG_SH3_3	179	185	PF00018	0.527
LIG_SH3_3	257	263	PF00018	0.506
LIG_SH3_3	381	387	PF00018	0.551
LIG_SH3_3	57	63	PF00018	0.345
LIG_SUMO_SIM_anti_2	338	344	PF11976	0.533
LIG_SUMO_SIM_par_1	153	159	PF11976	0.245
LIG_SUMO_SIM_par_1	265	270	PF11976	0.371
LIG_SUMO_SIM_par_1	360	366	PF11976	0.528
LIG_SUMO_SIM_par_1	412	417	PF11976	0.452
LIG_SUMO_SIM_par_1	95	100	PF11976	0.566
LIG_TYR_ITIM	50	55	PF00017	0.453
MOD_CK1_1	156	162	PF00069	0.405
MOD_CK1_1	177	183	PF00069	0.397
MOD_CK1_1	213	219	PF00069	0.354
MOD_CK1_1	46	52	PF00069	0.523
MOD_CK2_1	199	205	PF00069	0.238
MOD_CK2_1	213	219	PF00069	0.302
MOD_CK2_1	281	287	PF00069	0.432
MOD_CK2_1	348	354	PF00069	0.514
MOD_CK2_1	439	445	PF00069	0.474
MOD_CK2_1	475	481	PF00069	0.501
MOD_GlcNHglycan	242	245	PF01048	0.628
MOD_GlcNHglycan	416	419	PF01048	0.419
MOD_GlcNHglycan	441	444	PF01048	0.453
MOD_GlcNHglycan	78	81	PF01048	0.609
MOD_GSK3_1	105	112	PF00069	0.214
MOD_GSK3_1	156	163	PF00069	0.415
MOD_GSK3_1	177	184	PF00069	0.495
MOD_GSK3_1	273	280	PF00069	0.359
MOD_GSK3_1	344	351	PF00069	0.529
MOD_GSK3_1	372	379	PF00069	0.532
MOD_GSK3_1	42	49	PF00069	0.439
MOD_GSK3_1	425	432	PF00069	0.511
MOD_GSK3_1	439	446	PF00069	0.516
MOD_GSK3_1	85	92	PF00069	0.697
MOD_NEK2_1	22	27	PF00069	0.436
MOD_NEK2_1	331	336	PF00069	0.488
MOD_NEK2_1	56	61	PF00069	0.356
MOD_NEK2_1	97	102	PF00069	0.543
MOD_NEK2_2	475	480	PF00069	0.489
MOD_PKA_1	151	157	PF00069	0.347
MOD_PKA_1	358	364	PF00069	0.516
MOD_PKA_2	225	231	PF00069	0.408
MOD_PKA_2	344	350	PF00069	0.532
MOD_PKA_2	358	364	PF00069	0.516
MOD_PKA_2	42	48	PF00069	0.408
MOD_PKA_2	422	428	PF00069	0.621
MOD_Plk_2-3	363	369	PF00069	0.444
MOD_Plk_4	14	20	PF00069	0.403
MOD_Plk_4	22	28	PF00069	0.395
MOD_Plk_4	225	231	PF00069	0.303
MOD_Plk_4	298	304	PF00069	0.521
MOD_Plk_4	338	344	PF00069	0.564
MOD_Plk_4	481	487	PF00069	0.449
MOD_Plk_4	56	62	PF00069	0.355
MOD_ProDKin_1	110	116	PF00069	0.465
MOD_ProDKin_1	181	187	PF00069	0.502
MOD_ProDKin_1	443	449	PF00069	0.550
MOD_SUMO_rev_2	79	85	PF00179	0.457
TRG_DiLeu_BaEn_1	481	486	PF01217	0.508
TRG_ENDOCYTIC_2	168	171	PF00928	0.388
TRG_ENDOCYTIC_2	52	55	PF00928	0.414
TRG_ENDOCYTIC_2	72	75	PF00928	0.295
TRG_ER_diArg_1	102	104	PF00400	0.461
TRG_ER_diArg_1	130	132	PF00400	0.423
TRG_ER_diArg_1	251	254	PF00400	0.614
TRG_ER_diArg_1	388	390	PF00400	0.403
TRG_ER_diArg_1	401	404	PF00400	0.389
TRG_ER_diArg_1	410	412	PF00400	0.463
TRG_Pf-PMV_PEXEL_1	254	258	PF00026	0.574
TRG_Pf-PMV_PEXEL_1	325	330	PF00026	0.479

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1V1	Leptomonas seymouri	73%	99%
A0A0S4J566	Bodo saltans	43%	100%
A0A1X0NTW0	Trypanosomatidae	43%	100%
A0A3S7WTE7	Leishmania donovani	93%	100%
A0A422NRG2	Trypanosoma rangeli	42%	100%
A4H839	Leishmania braziliensis	82%	100%
A4HWG6	Leishmania infantum	93%	100%
C9ZXY1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
O57734	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	24%	100%
P53960	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q08B09	Xenopus laevis	28%	100%
Q3THG9	Mus musculus	30%	100%
Q4QFB6	Leishmania major	93%	100%
Q5XI97	Rattus norvegicus	30%	100%
Q6DEJ5	Danio rerio	30%	100%
Q7ZYJ9	Xenopus laevis	29%	100%
Q9BTE6	Homo sapiens	29%	100%