Putative tyrosyl-tRNA synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tyrosyl-tRNA synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9APY5_LEIMU

TriTrypDb:

LmxM.14.1370

Length:

682

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9APY5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APY5

PDB structure(s): 3p0h_A , 3p0h_B , 3p0i_A , 3p0i_B , 3p0j_A , 3p0j_B , 3p0j_C , 3p0j_D , 5usf_A , 5usf_B

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006399	tRNA metabolic process	7	12
GO:0006418	tRNA aminoacylation for protein translation	6	12
GO:0006520	amino acid metabolic process	3	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0019752	carboxylic acid metabolic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	12
GO:0043038	amino acid activation	4	12
GO:0043039	tRNA aminoacylation	5	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043436	oxoacid metabolic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044281	small molecule metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901564	organonitrogen compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004812	aminoacyl-tRNA ligase activity	4	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0004831	tyrosine-tRNA ligase activity	5	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	226	230	PF00656	0.454
CLV_C14_Caspase3-7	519	523	PF00656	0.492
CLV_NRD_NRD_1	185	187	PF00675	0.254
CLV_NRD_NRD_1	200	202	PF00675	0.254
CLV_NRD_NRD_1	242	244	PF00675	0.254
CLV_NRD_NRD_1	353	355	PF00675	0.462
CLV_NRD_NRD_1	476	478	PF00675	0.329
CLV_NRD_NRD_1	576	578	PF00675	0.377
CLV_PCSK_KEX2_1	576	578	PF00082	0.380
CLV_PCSK_KEX2_1	680	682	PF00082	0.586
CLV_PCSK_PC1ET2_1	680	682	PF00082	0.586
CLV_PCSK_SKI1_1	109	113	PF00082	0.279
CLV_PCSK_SKI1_1	128	132	PF00082	0.267
CLV_PCSK_SKI1_1	201	205	PF00082	0.265
CLV_PCSK_SKI1_1	453	457	PF00082	0.475
CLV_PCSK_SKI1_1	504	508	PF00082	0.386
CLV_PCSK_SKI1_1	577	581	PF00082	0.394
CLV_PCSK_SKI1_1	657	661	PF00082	0.455
CLV_PCSK_SKI1_1	666	670	PF00082	0.538
CLV_PCSK_SKI1_1	677	681	PF00082	0.538
CLV_Separin_Metazoa	331	335	PF03568	0.335
DEG_Nend_UBRbox_3	1	3	PF02207	0.521
DEG_SPOP_SBC_1	516	520	PF00917	0.418
DOC_CKS1_1	621	626	PF01111	0.486
DOC_CYCLIN_yCln2_LP_2	384	390	PF00134	0.457
DOC_MAPK_gen_1	201	208	PF00069	0.465
DOC_MAPK_gen_1	354	362	PF00069	0.638
DOC_MAPK_MEF2A_6	504	513	PF00069	0.524
DOC_MAPK_MEF2A_6	642	650	PF00069	0.393
DOC_PP1_RVXF_1	107	113	PF00149	0.454
DOC_PP2B_LxvP_1	384	387	PF13499	0.466
DOC_PP2B_LxvP_1	412	415	PF13499	0.301
DOC_PP2B_PxIxI_1	591	597	PF00149	0.493
DOC_USP7_MATH_1	221	225	PF00917	0.454
DOC_USP7_MATH_1	307	311	PF00917	0.520
DOC_USP7_MATH_1	317	321	PF00917	0.435
DOC_USP7_MATH_1	349	353	PF00917	0.488
DOC_USP7_MATH_1	419	423	PF00917	0.516
DOC_USP7_UBL2_3	351	355	PF12436	0.464
DOC_USP7_UBL2_3	676	680	PF12436	0.435
DOC_WW_Pin1_4	620	625	PF00397	0.491
LIG_14-3-3_CanoR_1	252	260	PF00244	0.571
LIG_14-3-3_CanoR_1	480	488	PF00244	0.253
LIG_14-3-3_CanoR_1	504	513	PF00244	0.461
LIG_BRCT_BRCA1_1	229	233	PF00533	0.454
LIG_CSL_BTD_1	378	381	PF09270	0.323
LIG_FHA_1	357	363	PF00498	0.615
LIG_FHA_1	409	415	PF00498	0.457
LIG_FHA_1	493	499	PF00498	0.385
LIG_FHA_2	235	241	PF00498	0.465
LIG_FHA_2	298	304	PF00498	0.460
LIG_FHA_2	424	430	PF00498	0.413
LIG_FHA_2	454	460	PF00498	0.382
LIG_FHA_2	517	523	PF00498	0.398
LIG_KLC1_Yacidic_2	564	569	PF13176	0.484
LIG_LIR_Apic_2	620	624	PF02991	0.547
LIG_LIR_Gen_1	194	203	PF02991	0.454
LIG_LIR_Gen_1	277	287	PF02991	0.478
LIG_LIR_Gen_1	300	309	PF02991	0.465
LIG_LIR_Gen_1	416	425	PF02991	0.337
LIG_LIR_Gen_1	467	476	PF02991	0.396
LIG_LIR_Gen_1	73	82	PF02991	0.454
LIG_LIR_Nem_3	124	130	PF02991	0.480
LIG_LIR_Nem_3	194	198	PF02991	0.454
LIG_LIR_Nem_3	277	282	PF02991	0.465
LIG_LIR_Nem_3	296	301	PF02991	0.392
LIG_LIR_Nem_3	4	10	PF02991	0.354
LIG_LIR_Nem_3	416	420	PF02991	0.338
LIG_LIR_Nem_3	467	472	PF02991	0.466
LIG_LIR_Nem_3	73	77	PF02991	0.454
LIG_LYPXL_S_1	162	166	PF13949	0.254
LIG_LYPXL_yS_3	163	166	PF13949	0.454
LIG_NRBOX	645	651	PF00104	0.393
LIG_NRP_CendR_1	680	682	PF00754	0.571
LIG_REV1ctd_RIR_1	170	179	PF16727	0.454
LIG_SH2_CRK	284	288	PF00017	0.483
LIG_SH2_CRK	446	450	PF00017	0.380
LIG_SH2_CRK	583	587	PF00017	0.394
LIG_SH2_CRK	7	11	PF00017	0.369
LIG_SH2_NCK_1	284	288	PF00017	0.478
LIG_SH2_NCK_1	583	587	PF00017	0.536
LIG_SH2_NCK_1	621	625	PF00017	0.548
LIG_SH2_STAT5	195	198	PF00017	0.454
LIG_SH2_STAT5	249	252	PF00017	0.465
LIG_SH2_STAT5	278	281	PF00017	0.505
LIG_SH2_STAT5	532	535	PF00017	0.512
LIG_SH2_STAT5	567	570	PF00017	0.429
LIG_SH2_STAT5	95	98	PF00017	0.460
LIG_SH3_2	368	373	PF14604	0.558
LIG_SH3_3	158	164	PF00018	0.521
LIG_SH3_3	365	371	PF00018	0.480
LIG_SH3_5	274	278	PF00018	0.454
LIG_SUMO_SIM_par_1	204	209	PF11976	0.454
LIG_SUMO_SIM_par_1	386	392	PF11976	0.461
LIG_SUMO_SIM_par_1	410	416	PF11976	0.349
LIG_SUMO_SIM_par_1	509	514	PF11976	0.410
LIG_TRAF2_1	115	118	PF00917	0.512
LIG_TRAF2_1	586	589	PF00917	0.489
LIG_UBA3_1	171	175	PF00899	0.454
LIG_UBA3_1	212	217	PF00899	0.433
MOD_CK1_1	620	626	PF00069	0.535
MOD_CK2_1	112	118	PF00069	0.454
MOD_CK2_1	336	342	PF00069	0.489
MOD_CK2_1	423	429	PF00069	0.434
MOD_CK2_1	453	459	PF00069	0.390
MOD_CK2_1	479	485	PF00069	0.518
MOD_CK2_1	9	15	PF00069	0.374
MOD_Cter_Amidation	199	202	PF01082	0.254
MOD_GlcNHglycan	229	232	PF01048	0.273
MOD_GlcNHglycan	255	258	PF01048	0.362
MOD_GlcNHglycan	40	44	PF01048	0.254
MOD_GlcNHglycan	416	420	PF01048	0.410
MOD_GlcNHglycan	431	435	PF01048	0.326
MOD_GlcNHglycan	583	586	PF01048	0.530
MOD_GlcNHglycan	632	635	PF01048	0.408
MOD_GlcNHglycan	651	655	PF01048	0.370
MOD_GSK3_1	113	120	PF00069	0.465
MOD_GSK3_1	223	230	PF00069	0.454
MOD_GSK3_1	289	296	PF00069	0.475
MOD_GSK3_1	307	314	PF00069	0.521
MOD_GSK3_1	415	422	PF00069	0.449
MOD_GSK3_1	511	518	PF00069	0.333
MOD_N-GLC_1	617	622	PF02516	0.422
MOD_N-GLC_1	666	671	PF02516	0.422
MOD_N-GLC_2	58	60	PF02516	0.254
MOD_NEK2_1	362	367	PF00069	0.539
MOD_NEK2_1	436	441	PF00069	0.225
MOD_NEK2_1	468	473	PF00069	0.505
MOD_NEK2_1	509	514	PF00069	0.344
MOD_NEK2_1	641	646	PF00069	0.436
MOD_NEK2_1	9	14	PF00069	0.357
MOD_PKA_2	479	485	PF00069	0.240
MOD_PKA_2	492	498	PF00069	0.274
MOD_Plk_1	415	421	PF00069	0.320
MOD_Plk_1	617	623	PF00069	0.423
MOD_Plk_1	666	672	PF00069	0.476
MOD_Plk_2-3	311	317	PF00069	0.531
MOD_Plk_4	122	128	PF00069	0.482
MOD_Plk_4	144	150	PF00069	0.463
MOD_Plk_4	408	414	PF00069	0.469
MOD_Plk_4	468	474	PF00069	0.468
MOD_Plk_4	641	647	PF00069	0.250
MOD_ProDKin_1	620	626	PF00069	0.489
MOD_SUMO_rev_2	22	32	PF00179	0.428
TRG_DiLeu_BaEn_1	194	199	PF01217	0.470
TRG_DiLeu_BaEn_1	22	27	PF01217	0.372
TRG_DiLeu_BaEn_1	325	330	PF01217	0.493
TRG_DiLeu_BaEn_1	342	347	PF01217	0.339
TRG_DiLeu_BaEn_1	5	10	PF01217	0.344
TRG_DiLeu_BaEn_1	655	660	PF01217	0.257
TRG_DiLeu_LyEn_5	28	33	PF01217	0.405
TRG_ENDOCYTIC_2	126	129	PF00928	0.482
TRG_ENDOCYTIC_2	163	166	PF00928	0.454
TRG_ENDOCYTIC_2	195	198	PF00928	0.454
TRG_ENDOCYTIC_2	301	304	PF00928	0.454
TRG_ENDOCYTIC_2	36	39	PF00928	0.454
TRG_ENDOCYTIC_2	7	10	PF00928	0.389
TRG_ER_diArg_1	575	577	PF00400	0.432
TRG_NES_CRM1_1	640	651	PF08389	0.432
TRG_NLS_Bipartite_1	186	205	PF00514	0.454
TRG_NLS_MonoExtC_3	200	205	PF00514	0.454
TRG_NLS_MonoExtC_3	576	581	PF00514	0.395
TRG_NLS_MonoExtN_4	576	581	PF00514	0.410
TRG_Pf-PMV_PEXEL_1	24	28	PF00026	0.472
TRG_Pf-PMV_PEXEL_1	243	247	PF00026	0.355
TRG_Pf-PMV_PEXEL_1	94	98	PF00026	0.321

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3B3	Leptomonas seymouri	82%	100%
A0A0S4JQB1	Bodo saltans	61%	76%
A0A1X0NNR8	Trypanosomatidae	63%	98%
A0A3Q8ID88	Leishmania donovani	95%	100%
A0A422NFL4	Trypanosoma rangeli	61%	99%
A4H7V7	Leishmania braziliensis	88%	100%
A4HW83	Leishmania infantum	95%	100%
C9ZST4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	61%	98%
F4HWL4	Arabidopsis thaliana	36%	91%
Q4QFJ7	Leishmania major	96%	100%
V5AW05	Trypanosoma cruzi	61%	99%