Poly(A) polymerase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Poly(A) polymerase

Gene product:

polynucleotide adenylyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9APW5_LEIMU

TriTrypDb:

LmxM.14.1180

Length:

706

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005737	cytoplasm	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9APW5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APW5

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006397	mRNA processing	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0016071	mRNA metabolic process	6	12
GO:0031123	RNA 3'-end processing	7	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0043170	macromolecule metabolic process	3	12
GO:0043631	RNA polyadenylation	6	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071076	RNA 3' uridylation	8	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:0006378	mRNA polyadenylation	7	1
GO:0031124	mRNA 3'-end processing	8	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004652	obsolete polynucleotide adenylyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0050265	RNA uridylyltransferase activity	4	12
GO:0070566	adenylyltransferase activity	5	12
GO:0070569	uridylyltransferase activity	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	302	306	PF00656	0.317
CLV_C14_Caspase3-7	388	392	PF00656	0.296
CLV_C14_Caspase3-7	622	626	PF00656	0.768
CLV_C14_Caspase3-7	646	650	PF00656	0.772
CLV_NRD_NRD_1	50	52	PF00675	0.308
CLV_NRD_NRD_1	652	654	PF00675	0.679
CLV_PCSK_KEX2_1	49	51	PF00082	0.308
CLV_PCSK_KEX2_1	654	656	PF00082	0.730
CLV_PCSK_PC1ET2_1	654	656	PF00082	0.730
CLV_PCSK_SKI1_1	204	208	PF00082	0.313
CLV_PCSK_SKI1_1	212	216	PF00082	0.283
CLV_PCSK_SKI1_1	51	55	PF00082	0.301
CLV_PCSK_SKI1_1	9	13	PF00082	0.470
DEG_APCC_DBOX_1	207	215	PF00400	0.301
DEG_APCC_DBOX_1	50	58	PF00400	0.301
DEG_SPOP_SBC_1	602	606	PF00917	0.754
DOC_CKS1_1	312	317	PF01111	0.301
DOC_MAPK_DCC_7	140	149	PF00069	0.317
DOC_MAPK_FxFP_2	228	231	PF00069	0.317
DOC_MAPK_gen_1	300	309	PF00069	0.317
DOC_MAPK_HePTP_8	53	65	PF00069	0.421
DOC_MAPK_MEF2A_6	140	149	PF00069	0.317
DOC_MAPK_MEF2A_6	378	387	PF00069	0.324
DOC_MAPK_MEF2A_6	56	65	PF00069	0.404
DOC_PP1_RVXF_1	405	411	PF00149	0.324
DOC_PP2B_LxvP_1	108	111	PF13499	0.448
DOC_PP2B_PxIxI_1	541	547	PF00149	0.317
DOC_PP4_FxxP_1	228	231	PF00568	0.317
DOC_PP4_FxxP_1	436	439	PF00568	0.386
DOC_PP4_FxxP_1	73	76	PF00568	0.317
DOC_USP7_MATH_1	299	303	PF00917	0.317
DOC_USP7_MATH_1	613	617	PF00917	0.776
DOC_USP7_MATH_1	624	628	PF00917	0.785
DOC_USP7_MATH_1	644	648	PF00917	0.652
DOC_USP7_MATH_1	678	682	PF00917	0.734
DOC_USP7_MATH_1	685	689	PF00917	0.678
DOC_USP7_MATH_1	89	93	PF00917	0.460
DOC_USP7_MATH_2	299	305	PF00917	0.317
DOC_USP7_UBL2_3	215	219	PF12436	0.301
DOC_WW_Pin1_4	311	316	PF00397	0.301
LIG_14-3-3_CanoR_1	208	212	PF00244	0.412
LIG_14-3-3_CanoR_1	258	264	PF00244	0.345
LIG_14-3-3_CanoR_1	288	297	PF00244	0.421
LIG_14-3-3_CanoR_1	417	422	PF00244	0.358
LIG_14-3-3_CanoR_1	460	464	PF00244	0.156
LIG_14-3-3_CanoR_1	49	54	PF00244	0.315
LIG_14-3-3_CanoR_1	522	528	PF00244	0.409
LIG_14-3-3_CanoR_1	66	70	PF00244	0.242
LIG_BIR_II_1	1	5	PF00653	0.571
LIG_BIR_III_3	1	5	PF00653	0.487
LIG_BRCT_BRCA1_1	224	228	PF00533	0.317
LIG_EH1_1	194	202	PF00400	0.335
LIG_FHA_1	171	177	PF00498	0.301
LIG_FHA_1	238	244	PF00498	0.421
LIG_FHA_1	312	318	PF00498	0.312
LIG_FHA_1	674	680	PF00498	0.615
LIG_FHA_2	272	278	PF00498	0.386
LIG_FHA_2	3	9	PF00498	0.647
LIG_FHA_2	37	43	PF00498	0.397
LIG_FHA_2	418	424	PF00498	0.396
LIG_FHA_2	543	549	PF00498	0.302
LIG_LIR_Apic_2	225	231	PF02991	0.317
LIG_LIR_Apic_2	265	269	PF02991	0.336
LIG_LIR_Apic_2	71	76	PF02991	0.316
LIG_LIR_Gen_1	117	127	PF02991	0.448
LIG_LIR_Gen_1	523	531	PF02991	0.386
LIG_LIR_Nem_3	117	123	PF02991	0.448
LIG_LIR_Nem_3	157	163	PF02991	0.301
LIG_LIR_Nem_3	257	263	PF02991	0.421
LIG_LIR_Nem_3	393	398	PF02991	0.341
LIG_LIR_Nem_3	422	427	PF02991	0.358
LIG_LIR_Nem_3	523	527	PF02991	0.386
LIG_MYND_1	311	315	PF01753	0.317
LIG_PDZ_Class_2	701	706	PF00595	0.540
LIG_Pex14_2	213	217	PF04695	0.301
LIG_Pex14_2	375	379	PF04695	0.448
LIG_PTB_Apo_2	318	325	PF02174	0.301
LIG_PTB_Phospho_1	318	324	PF10480	0.335
LIG_REV1ctd_RIR_1	257	265	PF16727	0.296
LIG_REV1ctd_RIR_1	373	382	PF16727	0.421
LIG_SH2_CRK	424	428	PF00017	0.421
LIG_SH2_GRB2like	224	227	PF00017	0.317
LIG_SH2_SRC	398	401	PF00017	0.412
LIG_SH2_STAP1	224	228	PF00017	0.317
LIG_SH2_STAT3	247	250	PF00017	0.421
LIG_SH2_STAT5	282	285	PF00017	0.317
LIG_SH2_STAT5	382	385	PF00017	0.301
LIG_SH2_STAT5	500	503	PF00017	0.304
LIG_SH2_STAT5	562	565	PF00017	0.535
LIG_SH3_3	309	315	PF00018	0.301
LIG_SH3_3	366	372	PF00018	0.475
LIG_SUMO_SIM_par_1	542	548	PF11976	0.286
LIG_TRAF2_1	43	46	PF00917	0.380
LIG_TRAF2_1	568	571	PF00917	0.629
LIG_UBA3_1	197	204	PF00899	0.317
LIG_WRC_WIRS_1	521	526	PF05994	0.386
LIG_WRC_WIRS_1	552	557	PF05994	0.421
MOD_CK1_1	112	118	PF00069	0.534
MOD_CK1_1	159	165	PF00069	0.295
MOD_CK1_1	184	190	PF00069	0.301
MOD_CK1_1	222	228	PF00069	0.448
MOD_CK1_1	450	456	PF00069	0.343
MOD_CK1_1	462	468	PF00069	0.242
MOD_CK1_1	487	493	PF00069	0.376
MOD_CK1_1	585	591	PF00069	0.761
MOD_CK1_1	607	613	PF00069	0.746
MOD_CK1_1	616	622	PF00069	0.732
MOD_CK1_1	629	635	PF00069	0.688
MOD_CK1_1	647	653	PF00069	0.692
MOD_CK2_1	271	277	PF00069	0.335
MOD_CK2_1	29	35	PF00069	0.405
MOD_CK2_1	349	355	PF00069	0.447
MOD_CK2_1	36	42	PF00069	0.347
MOD_CK2_1	387	393	PF00069	0.301
MOD_CK2_1	49	55	PF00069	0.117
MOD_CK2_1	571	577	PF00069	0.712
MOD_CK2_1	624	630	PF00069	0.748
MOD_GlcNHglycan	17	20	PF01048	0.419
MOD_GlcNHglycan	2	5	PF01048	0.568
MOD_GlcNHglycan	231	234	PF01048	0.301
MOD_GlcNHglycan	351	354	PF01048	0.500
MOD_GlcNHglycan	467	470	PF01048	0.367
MOD_GlcNHglycan	486	490	PF01048	0.344
MOD_GlcNHglycan	511	514	PF01048	0.337
MOD_GlcNHglycan	589	592	PF01048	0.674
MOD_GlcNHglycan	621	624	PF01048	0.667
MOD_GlcNHglycan	625	629	PF01048	0.634
MOD_GlcNHglycan	680	683	PF01048	0.710
MOD_GlcNHglycan	687	690	PF01048	0.640
MOD_GlcNHglycan	99	102	PF01048	0.248
MOD_GSK3_1	413	420	PF00069	0.343
MOD_GSK3_1	443	450	PF00069	0.368
MOD_GSK3_1	454	461	PF00069	0.276
MOD_GSK3_1	474	481	PF00069	0.362
MOD_GSK3_1	484	491	PF00069	0.418
MOD_GSK3_1	505	512	PF00069	0.404
MOD_GSK3_1	597	604	PF00069	0.684
MOD_GSK3_1	613	620	PF00069	0.788
MOD_GSK3_1	624	631	PF00069	0.733
MOD_GSK3_1	643	650	PF00069	0.683
MOD_GSK3_1	674	681	PF00069	0.685
MOD_LATS_1	651	657	PF00433	0.601
MOD_N-GLC_1	324	329	PF02516	0.301
MOD_N-GLC_1	412	417	PF02516	0.421
MOD_N-GLC_1	582	587	PF02516	0.778
MOD_N-GLC_2	321	323	PF02516	0.301
MOD_NEK2_1	119	124	PF00069	0.448
MOD_NEK2_1	207	212	PF00069	0.425
MOD_NEK2_1	259	264	PF00069	0.421
MOD_NEK2_1	283	288	PF00069	0.301
MOD_NEK2_1	349	354	PF00069	0.556
MOD_NEK2_2	319	324	PF00069	0.448
MOD_NEK2_2	520	525	PF00069	0.448
MOD_PK_1	219	225	PF00069	0.448
MOD_PK_1	49	55	PF00069	0.320
MOD_PKA_1	49	55	PF00069	0.335
MOD_PKA_1	653	659	PF00069	0.753
MOD_PKA_2	112	118	PF00069	0.448
MOD_PKA_2	139	145	PF00069	0.358
MOD_PKA_2	207	213	PF00069	0.370
MOD_PKA_2	299	305	PF00069	0.317
MOD_PKA_2	446	452	PF00069	0.362
MOD_PKA_2	459	465	PF00069	0.349
MOD_PKA_2	49	55	PF00069	0.302
MOD_PKA_2	65	71	PF00069	0.256
MOD_PKB_1	445	453	PF00069	0.421
MOD_Plk_1	156	162	PF00069	0.301
MOD_Plk_1	181	187	PF00069	0.302
MOD_Plk_1	29	35	PF00069	0.450
MOD_Plk_1	324	330	PF00069	0.317
MOD_Plk_2-3	170	176	PF00069	0.301
MOD_Plk_4	181	187	PF00069	0.312
MOD_Plk_4	262	268	PF00069	0.335
MOD_Plk_4	491	497	PF00069	0.421
MOD_Plk_4	523	529	PF00069	0.368
MOD_Plk_4	551	557	PF00069	0.408
MOD_Plk_4	65	71	PF00069	0.301
MOD_ProDKin_1	311	317	PF00069	0.301
MOD_SUMO_for_1	127	130	PF00179	0.534
MOD_SUMO_for_1	670	673	PF00179	0.751
MOD_SUMO_rev_2	298	304	PF00179	0.318
MOD_SUMO_rev_2	352	358	PF00179	0.420
TRG_ENDOCYTIC_2	382	385	PF00928	0.307
TRG_ENDOCYTIC_2	424	427	PF00928	0.421
TRG_ER_diArg_1	287	290	PF00400	0.448
TRG_ER_diArg_1	405	408	PF00400	0.421
TRG_ER_diArg_1	444	447	PF00400	0.296
TRG_ER_diArg_1	49	51	PF00400	0.317
TRG_NLS_MonoExtC_3	652	657	PF00514	0.737
TRG_Pf-PMV_PEXEL_1	332	336	PF00026	0.295
TRG_Pf-PMV_PEXEL_1	529	533	PF00026	0.317
TRG_Pf-PMV_PEXEL_1	633	637	PF00026	0.699

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I2K1	Leptomonas seymouri	73%	96%
A0A0S4JIG5	Bodo saltans	47%	100%
A0A1X0NP17	Trypanosomatidae	61%	100%
A0A3Q8ID77	Leishmania donovani	94%	100%
A0A3R7KE83	Trypanosoma rangeli	66%	100%
A4H7T8	Leishmania braziliensis	87%	100%
A4HW64	Leishmania infantum	94%	100%
A4I4T6	Leishmania infantum	26%	100%
C9ZSW0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9AEC2	Leishmania major	27%	100%
E9ALG4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
P29468	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	28%	100%
Q4QFL6	Leishmania major	94%	99%
V5BKK6	Trypanosoma cruzi	59%	86%