PRK domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9APU2_LEIMU

TriTrypDb:

LmxM.14.0970

Length:

287

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9APU2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APU2

Function

Biological processes
Term	Name	Level	Count
GO:0006793	phosphorus metabolic process	3	1
GO:0006796	phosphate-containing compound metabolic process	4	1
GO:0008152	metabolic process	1	1
GO:0009987	cellular process	1	1
GO:0016310	phosphorylation	5	1
GO:0044237	cellular metabolic process	2	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	4
GO:0003824	catalytic activity	1	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0016301	kinase activity	4	4
GO:0016740	transferase activity	2	4
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	88	92	PF00656	0.573
CLV_NRD_NRD_1	124	126	PF00675	0.374
CLV_NRD_NRD_1	140	142	PF00675	0.482
CLV_NRD_NRD_1	247	249	PF00675	0.283
CLV_NRD_NRD_1	84	86	PF00675	0.596
CLV_PCSK_KEX2_1	139	141	PF00082	0.408
CLV_PCSK_PC1ET2_1	139	141	PF00082	0.440
CLV_PCSK_SKI1_1	126	130	PF00082	0.506
CLV_PCSK_SKI1_1	222	226	PF00082	0.308
CLV_PCSK_SKI1_1	239	243	PF00082	0.308
CLV_PCSK_SKI1_1	71	75	PF00082	0.385
DEG_APCC_DBOX_1	124	132	PF00400	0.344
DEG_APCC_DBOX_1	152	160	PF00400	0.359
DEG_COP1_1	169	176	PF00400	0.374
DOC_CKS1_1	159	164	PF01111	0.428
DOC_MAPK_DCC_7	40	49	PF00069	0.411
DOC_MAPK_gen_1	151	159	PF00069	0.393
DOC_MAPK_MEF2A_6	40	49	PF00069	0.487
DOC_PP2B_LxvP_1	129	132	PF13499	0.430
DOC_USP7_MATH_1	255	259	PF00917	0.413
DOC_WW_Pin1_4	158	163	PF00397	0.419
DOC_WW_Pin1_4	206	211	PF00397	0.427
LIG_14-3-3_CanoR_1	20	25	PF00244	0.446
LIG_Actin_WH2_2	17	35	PF00022	0.389
LIG_BIR_II_1	1	5	PF00653	0.561
LIG_deltaCOP1_diTrp_1	144	147	PF00928	0.321
LIG_deltaCOP1_diTrp_1	211	219	PF00928	0.462
LIG_FHA_1	231	237	PF00498	0.427
LIG_FHA_2	159	165	PF00498	0.434
LIG_LIR_Gen_1	209	219	PF02991	0.420
LIG_LIR_Nem_3	119	124	PF02991	0.311
LIG_LIR_Nem_3	200	206	PF02991	0.413
LIG_LIR_Nem_3	209	215	PF02991	0.413
LIG_PDZ_Class_1	282	287	PF00595	0.614
LIG_Rb_pABgroove_1	213	221	PF01858	0.462
LIG_SH2_SRC	168	171	PF00017	0.426
LIG_SH2_STAP1	168	172	PF00017	0.449
LIG_SH2_STAT3	25	28	PF00017	0.472
LIG_SH2_STAT5	203	206	PF00017	0.440
LIG_SH3_2	80	85	PF14604	0.543
LIG_SH3_3	168	174	PF00018	0.372
LIG_SH3_3	177	183	PF00018	0.483
LIG_SH3_3	77	83	PF00018	0.543
LIG_SUMO_SIM_anti_2	60	68	PF11976	0.305
LIG_SUMO_SIM_par_1	154	161	PF11976	0.367
LIG_SUMO_SIM_par_1	195	200	PF11976	0.427
LIG_SUMO_SIM_par_1	20	26	PF11976	0.488
LIG_TRAF2_1	10	13	PF00917	0.388
LIG_WRC_WIRS_1	173	178	PF05994	0.349
MOD_CDK_SPxxK_3	158	165	PF00069	0.435
MOD_CK1_1	57	63	PF00069	0.313
MOD_CK1_1	96	102	PF00069	0.770
MOD_CK2_1	158	164	PF00069	0.430
MOD_CK2_1	20	26	PF00069	0.401
MOD_CK2_1	244	250	PF00069	0.335
MOD_CK2_1	7	13	PF00069	0.391
MOD_GlcNHglycan	1	4	PF01048	0.567
MOD_GlcNHglycan	106	109	PF01048	0.541
MOD_GlcNHglycan	192	195	PF01048	0.371
MOD_GlcNHglycan	255	258	PF01048	0.404
MOD_GlcNHglycan	56	59	PF01048	0.308
MOD_GSK3_1	143	150	PF00069	0.387
MOD_GSK3_1	253	260	PF00069	0.293
MOD_GSK3_1	276	283	PF00069	0.407
MOD_GSK3_1	54	61	PF00069	0.312
MOD_LATS_1	5	11	PF00433	0.428
MOD_N-GLC_1	280	285	PF02516	0.535
MOD_N-GLC_1	96	101	PF02516	0.576
MOD_NEK2_1	265	270	PF00069	0.250
MOD_NEK2_1	32	37	PF00069	0.407
MOD_NEK2_2	155	160	PF00069	0.231
MOD_PK_1	20	26	PF00069	0.419
MOD_PKA_2	253	259	PF00069	0.325
MOD_PKA_2	32	38	PF00069	0.524
MOD_Plk_1	143	149	PF00069	0.331
MOD_Plk_1	184	190	PF00069	0.339
MOD_Plk_1	266	272	PF00069	0.403
MOD_Plk_4	20	26	PF00069	0.527
MOD_Plk_4	58	64	PF00069	0.424
MOD_ProDKin_1	158	164	PF00069	0.430
MOD_ProDKin_1	206	212	PF00069	0.267
MOD_SUMO_rev_2	217	224	PF00179	0.377
MOD_SUMO_rev_2	279	287	PF00179	0.572
TRG_ENDOCYTIC_2	168	171	PF00928	0.391
TRG_NES_CRM1_1	12	26	PF08389	0.359
TRG_NES_CRM1_1	79	93	PF08389	0.493
TRG_NLS_Bipartite_1	125	143	PF00514	0.311
TRG_NLS_MonoExtC_3	138	144	PF00514	0.311
TRG_Pf-PMV_PEXEL_1	165	169	PF00026	0.494
TRG_PTS1	284	287	PF00515	0.584

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PB45	Leptomonas seymouri	64%	98%
A0A0S4JJ70	Bodo saltans	36%	100%
A0A1X0NT00	Trypanosomatidae	46%	100%
A0A3Q8IA01	Leishmania donovani	94%	100%
A0A3R7K1F1	Trypanosoma rangeli	46%	100%
A4H7Q1	Leishmania braziliensis	82%	100%
A4HW39	Leishmania infantum	94%	100%
P11664	Escherichia coli (strain K12)	24%	100%
Q4QFN8	Leishmania major	93%	100%
Q9UTC5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
V5AZD0	Trypanosoma cruzi	51%	100%