Glutathione synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

glutathione synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9APT6_LEIMU

TriTrypDb:

LmxM.14.0910

Length:

608

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	1
GO:0005737	cytoplasm	2	1
GO:0005829	cytosol	2	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1

Expansion

Sequence features

E9APT6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APT6

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0000287	magnesium ion binding	5	12
GO:0003824	catalytic activity	1	12
GO:0004363	glutathione synthase activity	3	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0033218	amide binding	2	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0042277	peptide binding	3	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0043295	glutathione binding	3	12
GO:0046872	metal ion binding	4	12
GO:0072341	modified amino acid binding	2	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1900750	oligopeptide binding	4	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:1901681	sulfur compound binding	2	12
GO:1904091	non-ribosomal peptide synthetase activity	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	588	592	PF00656	0.340
CLV_NRD_NRD_1	317	319	PF00675	0.282
CLV_NRD_NRD_1	523	525	PF00675	0.314
CLV_NRD_NRD_1	530	532	PF00675	0.292
CLV_PCSK_KEX2_1	317	319	PF00082	0.270
CLV_PCSK_KEX2_1	523	525	PF00082	0.296
CLV_PCSK_KEX2_1	530	532	PF00082	0.292
CLV_PCSK_SKI1_1	104	108	PF00082	0.365
CLV_PCSK_SKI1_1	20	24	PF00082	0.463
CLV_PCSK_SKI1_1	201	205	PF00082	0.288
CLV_PCSK_SKI1_1	302	306	PF00082	0.370
CLV_PCSK_SKI1_1	346	350	PF00082	0.145
CLV_PCSK_SKI1_1	74	78	PF00082	0.292
DEG_APCC_DBOX_1	316	324	PF00400	0.268
DEG_SPOP_SBC_1	3	7	PF00917	0.596
DOC_CKS1_1	119	124	PF01111	0.396
DOC_CKS1_1	221	226	PF01111	0.236
DOC_CYCLIN_RxL_1	299	309	PF00134	0.355
DOC_CYCLIN_yCln2_LP_2	353	359	PF00134	0.340
DOC_MAPK_DCC_7	277	287	PF00069	0.340
DOC_MAPK_gen_1	199	207	PF00069	0.288
DOC_MAPK_JIP1_4	283	289	PF00069	0.396
DOC_MAPK_MEF2A_6	35	43	PF00069	0.344
DOC_MAPK_RevD_3	303	318	PF00069	0.317
DOC_PP1_RVXF_1	72	79	PF00149	0.312
DOC_PP1_SILK_1	135	140	PF00149	0.288
DOC_PP2B_LxvP_1	353	356	PF13499	0.340
DOC_PP4_FxxP_1	221	224	PF00568	0.145
DOC_USP7_MATH_1	266	270	PF00917	0.419
DOC_USP7_MATH_1	54	58	PF00917	0.244
DOC_USP7_MATH_1	577	581	PF00917	0.280
DOC_USP7_MATH_1	600	604	PF00917	0.266
DOC_WW_Pin1_4	118	123	PF00397	0.377
DOC_WW_Pin1_4	184	189	PF00397	0.291
DOC_WW_Pin1_4	220	225	PF00397	0.410
DOC_WW_Pin1_4	227	232	PF00397	0.401
LIG_14-3-3_CanoR_1	153	161	PF00244	0.220
LIG_14-3-3_CanoR_1	317	321	PF00244	0.269
LIG_14-3-3_CanoR_1	341	349	PF00244	0.366
LIG_14-3-3_CanoR_1	372	376	PF00244	0.270
LIG_Actin_WH2_2	301	319	PF00022	0.327
LIG_BIR_III_4	568	572	PF00653	0.331
LIG_BRCT_BRCA1_1	179	183	PF00533	0.255
LIG_BRCT_BRCA1_1	85	89	PF00533	0.288
LIG_FHA_1	105	111	PF00498	0.265
LIG_FHA_1	118	124	PF00498	0.288
LIG_FHA_1	221	227	PF00498	0.386
LIG_FHA_1	276	282	PF00498	0.313
LIG_FHA_1	308	314	PF00498	0.418
LIG_FHA_1	324	330	PF00498	0.270
LIG_FHA_1	343	349	PF00498	0.262
LIG_FHA_1	46	52	PF00498	0.370
LIG_FHA_1	535	541	PF00498	0.318
LIG_FHA_1	8	14	PF00498	0.480
LIG_GBD_Chelix_1	33	41	PF00786	0.292
LIG_LIR_Apic_2	220	224	PF02991	0.223
LIG_LIR_Gen_1	202	212	PF02991	0.288
LIG_LIR_Gen_1	402	413	PF02991	0.270
LIG_LIR_Gen_1	452	458	PF02991	0.285
LIG_LIR_Gen_1	516	527	PF02991	0.296
LIG_LIR_Gen_1	86	96	PF02991	0.405
LIG_LIR_Nem_3	180	186	PF02991	0.290
LIG_LIR_Nem_3	202	207	PF02991	0.288
LIG_LIR_Nem_3	299	304	PF02991	0.313
LIG_LIR_Nem_3	452	457	PF02991	0.285
LIG_LIR_Nem_3	516	522	PF02991	0.396
LIG_LIR_Nem_3	86	92	PF02991	0.361
LIG_NRBOX	303	309	PF00104	0.340
LIG_Pex14_2	200	204	PF04695	0.288
LIG_Rb_pABgroove_1	66	74	PF01858	0.379
LIG_SH2_CRK	130	134	PF00017	0.284
LIG_SH2_CRK	301	305	PF00017	0.348
LIG_SH2_CRK	454	458	PF00017	0.288
LIG_SH2_CRK	519	523	PF00017	0.270
LIG_SH2_STAP1	267	271	PF00017	0.288
LIG_SH2_STAP1	454	458	PF00017	0.288
LIG_SH2_STAP1	72	76	PF00017	0.379
LIG_SH2_STAT3	72	75	PF00017	0.396
LIG_SH2_STAT5	196	199	PF00017	0.320
LIG_SH2_STAT5	21	24	PF00017	0.341
LIG_SH2_STAT5	364	367	PF00017	0.341
LIG_SH2_STAT5	370	373	PF00017	0.243
LIG_SH2_STAT5	375	378	PF00017	0.220
LIG_SH2_STAT5	487	490	PF00017	0.289
LIG_SH2_STAT5	526	529	PF00017	0.281
LIG_SH3_3	126	132	PF00018	0.361
LIG_SH3_3	278	284	PF00018	0.227
LIG_SH3_3	395	401	PF00018	0.290
LIG_SH3_3	420	426	PF00018	0.270
LIG_SH3_3	478	484	PF00018	0.313
LIG_SH3_3	485	491	PF00018	0.263
LIG_SUMO_SIM_par_1	109	115	PF11976	0.340
LIG_SUMO_SIM_par_1	303	310	PF11976	0.431
LIG_TRAF2_1	27	30	PF00917	0.430
LIG_TRFH_1	487	491	PF08558	0.255
LIG_UBA3_1	110	116	PF00899	0.326
LIG_WRC_WIRS_1	267	272	PF05994	0.288
LIG_WRC_WIRS_1	407	412	PF05994	0.270
MOD_CDK_SPK_2	227	232	PF00069	0.314
MOD_CK1_1	152	158	PF00069	0.461
MOD_CK1_1	220	226	PF00069	0.455
MOD_CK1_1	342	348	PF00069	0.391
MOD_CK1_1	418	424	PF00069	0.408
MOD_CK1_1	95	101	PF00069	0.417
MOD_CK2_1	152	158	PF00069	0.395
MOD_CK2_1	25	31	PF00069	0.424
MOD_CK2_1	95	101	PF00069	0.365
MOD_GlcNHglycan	149	152	PF01048	0.368
MOD_GlcNHglycan	155	158	PF01048	0.358
MOD_GlcNHglycan	245	248	PF01048	0.316
MOD_GlcNHglycan	250	253	PF01048	0.276
MOD_GSK3_1	149	156	PF00069	0.326
MOD_GSK3_1	175	182	PF00069	0.265
MOD_GSK3_1	2	9	PF00069	0.701
MOD_GSK3_1	213	220	PF00069	0.387
MOD_GSK3_1	312	319	PF00069	0.274
MOD_GSK3_1	330	337	PF00069	0.270
MOD_GSK3_1	381	388	PF00069	0.363
MOD_GSK3_1	45	52	PF00069	0.359
MOD_GSK3_1	577	584	PF00069	0.412
MOD_GSK3_1	92	99	PF00069	0.376
MOD_N-GLC_1	418	423	PF02516	0.396
MOD_N-GLC_1	82	87	PF02516	0.295
MOD_N-GLC_2	553	555	PF02516	0.262
MOD_N-GLC_2	570	572	PF02516	0.199
MOD_NEK2_1	254	259	PF00069	0.211
MOD_NEK2_1	307	312	PF00069	0.323
MOD_NEK2_1	323	328	PF00069	0.247
MOD_NEK2_1	371	376	PF00069	0.266
MOD_NEK2_1	535	540	PF00069	0.362
MOD_NEK2_1	542	547	PF00069	0.382
MOD_NEK2_1	581	586	PF00069	0.296
MOD_NEK2_1	92	97	PF00069	0.297
MOD_NEK2_2	312	317	PF00069	0.329
MOD_NEK2_2	54	59	PF00069	0.396
MOD_PK_1	399	405	PF00069	0.270
MOD_PKA_2	152	158	PF00069	0.276
MOD_PKA_2	316	322	PF00069	0.270
MOD_PKA_2	340	346	PF00069	0.332
MOD_PKA_2	371	377	PF00069	0.270
MOD_PKA_2	83	89	PF00069	0.367
MOD_PKA_2	92	98	PF00069	0.292
MOD_Plk_2-3	511	517	PF00069	0.340
MOD_Plk_4	266	272	PF00069	0.288
MOD_Plk_4	371	377	PF00069	0.252
MOD_Plk_4	577	583	PF00069	0.368
MOD_ProDKin_1	118	124	PF00069	0.377
MOD_ProDKin_1	184	190	PF00069	0.291
MOD_ProDKin_1	220	226	PF00069	0.410
MOD_ProDKin_1	227	233	PF00069	0.401
TRG_DiLeu_BaLyEn_6	106	111	PF01217	0.348
TRG_DiLeu_BaLyEn_6	504	509	PF01217	0.374
TRG_ENDOCYTIC_2	130	133	PF00928	0.313
TRG_ENDOCYTIC_2	267	270	PF00928	0.288
TRG_ENDOCYTIC_2	301	304	PF00928	0.368
TRG_ENDOCYTIC_2	405	408	PF00928	0.304
TRG_ENDOCYTIC_2	454	457	PF00928	0.288
TRG_ENDOCYTIC_2	519	522	PF00928	0.255
TRG_ENDOCYTIC_2	526	529	PF00928	0.255
TRG_ER_diArg_1	316	318	PF00400	0.257
TRG_ER_diArg_1	461	464	PF00400	0.288
TRG_ER_diArg_1	522	524	PF00400	0.289
TRG_ER_diArg_1	529	531	PF00400	0.289
TRG_Pf-PMV_PEXEL_1	302	306	PF00026	0.365
TRG_Pf-PMV_PEXEL_1	318	322	PF00026	0.171
TRG_Pf-PMV_PEXEL_1	507	511	PF00026	0.396
TRG_Pf-PMV_PEXEL_1	69	73	PF00026	0.210

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IHC1	Leptomonas seymouri	63%	96%
A0A0S4J7U4	Bodo saltans	38%	100%
A0A1X0NNJ6	Trypanosomatidae	43%	100%
A0A3Q8IC15	Leishmania donovani	89%	100%
A0A3R7LQL5	Trypanosoma rangeli	46%	100%
A4H7P4	Leishmania braziliensis	74%	100%
A4HW34	Leishmania infantum	89%	100%
C9ZSZ1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
O22494	Solanum lycopersicum	29%	100%
O23732	Brassica juncea	29%	100%
P35668	Xenopus laevis	29%	100%
P35669	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	27%	100%
P46413	Rattus norvegicus	29%	100%
P46416	Arabidopsis thaliana	29%	100%
P48637	Homo sapiens	27%	100%
P51855	Mus musculus	29%	100%
Q08220	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	27%	100%
Q4QFP4	Leishmania major	87%	100%
Q54E83	Dictyostelium discoideum	25%	100%
Q5EAC2	Bos taurus	29%	100%
Q8HXX5	Macaca fascicularis	29%	100%
V5BNE3	Trypanosoma cruzi	47%	100%