SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9APQ4_LEIMU

TriTrypDb:

LmxM.14.0600

Length:

508

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9APQ4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APQ4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	8
GO:0006396	RNA processing	6	8
GO:0006725	cellular aromatic compound metabolic process	3	8
GO:0006807	nitrogen compound metabolic process	2	8
GO:0008152	metabolic process	1	8
GO:0009987	cellular process	1	8
GO:0016070	RNA metabolic process	5	8
GO:0034641	cellular nitrogen compound metabolic process	3	8
GO:0043170	macromolecule metabolic process	3	8
GO:0044237	cellular metabolic process	2	8
GO:0044238	primary metabolic process	2	8
GO:0046483	heterocycle metabolic process	3	8
GO:0071704	organic substance metabolic process	2	8
GO:0090304	nucleic acid metabolic process	4	8
GO:1901360	organic cyclic compound metabolic process	3	8
GO:0001510	RNA methylation	4	1
GO:0002128	tRNA nucleoside ribose methylation	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0008033	tRNA processing	8	1
GO:0009451	RNA modification	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0008168	methyltransferase activity	4	8
GO:0008173	RNA methyltransferase activity	4	8
GO:0016740	transferase activity	2	8
GO:0016741	transferase activity, transferring one-carbon groups	3	8
GO:0140098	catalytic activity, acting on RNA	3	8
GO:0140640	catalytic activity, acting on a nucleic acid	2	8
GO:0008171	O-methyltransferase activity	5	1
GO:0008175	tRNA methyltransferase activity	5	1
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	1
GO:0016300	tRNA (uracil) methyltransferase activity	6	1
GO:0016427	tRNA (cytosine) methyltransferase activity	6	1
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	1
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	1
GO:0062105	RNA 2'-O-methyltransferase activity	5	1
GO:0106050	tRNA 2'-O-methyltransferase activity	6	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0003676	nucleic acid binding	3	1
GO:0003723	RNA binding	4	1
GO:0005488	binding	1	1
GO:0097159	organic cyclic compound binding	2	1
GO:1901363	heterocyclic compound binding	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	302	308	PF00089	0.460
CLV_NRD_NRD_1	210	212	PF00675	0.489
CLV_NRD_NRD_1	314	316	PF00675	0.337
CLV_NRD_NRD_1	80	82	PF00675	0.585
CLV_NRD_NRD_1	95	97	PF00675	0.475
CLV_PCSK_FUR_1	93	97	PF00082	0.482
CLV_PCSK_KEX2_1	164	166	PF00082	0.697
CLV_PCSK_KEX2_1	210	212	PF00082	0.489
CLV_PCSK_KEX2_1	314	316	PF00082	0.337
CLV_PCSK_KEX2_1	95	97	PF00082	0.475
CLV_PCSK_PC1ET2_1	164	166	PF00082	0.697
CLV_PCSK_PC7_1	206	212	PF00082	0.352
CLV_PCSK_PC7_1	91	97	PF00082	0.464
CLV_PCSK_SKI1_1	206	210	PF00082	0.524
CLV_PCSK_SKI1_1	409	413	PF00082	0.575
CLV_PCSK_SKI1_1	489	493	PF00082	0.511
DEG_APCC_DBOX_1	205	213	PF00400	0.300
DEG_SPOP_SBC_1	369	373	PF00917	0.366
DEG_SPOP_SBC_1	66	70	PF00917	0.644
DOC_CKS1_1	241	246	PF01111	0.398
DOC_CYCLIN_yCln2_LP_2	101	107	PF00134	0.500
DOC_CYCLIN_yCln2_LP_2	259	265	PF00134	0.516
DOC_MAPK_gen_1	93	101	PF00069	0.452
DOC_MAPK_MEF2A_6	338	345	PF00069	0.446
DOC_PP1_SILK_1	248	253	PF00149	0.381
DOC_PP2B_LxvP_1	101	104	PF13499	0.415
DOC_PP2B_LxvP_1	343	346	PF13499	0.455
DOC_PP4_FxxP_1	395	398	PF00568	0.474
DOC_USP7_MATH_1	189	193	PF00917	0.609
DOC_USP7_MATH_1	281	285	PF00917	0.582
DOC_USP7_MATH_1	298	302	PF00917	0.519
DOC_USP7_MATH_1	369	373	PF00917	0.548
DOC_USP7_MATH_1	40	44	PF00917	0.807
DOC_USP7_MATH_1	66	70	PF00917	0.660
DOC_WW_Pin1_4	226	231	PF00397	0.603
DOC_WW_Pin1_4	240	245	PF00397	0.328
DOC_WW_Pin1_4	365	370	PF00397	0.706
DOC_WW_Pin1_4	42	47	PF00397	0.678
DOC_WW_Pin1_4	49	54	PF00397	0.604
DOC_WW_Pin1_4	503	508	PF00397	0.524
DOC_WW_Pin1_4	62	67	PF00397	0.667
LIG_14-3-3_CanoR_1	176	186	PF00244	0.739
LIG_14-3-3_CanoR_1	367	377	PF00244	0.341
LIG_BRCT_BRCA1_1	13	17	PF00533	0.355
LIG_Clathr_ClatBox_1	404	408	PF01394	0.399
LIG_FHA_1	117	123	PF00498	0.527
LIG_FHA_1	128	134	PF00498	0.523
LIG_FHA_1	271	277	PF00498	0.763
LIG_FHA_1	410	416	PF00498	0.632
LIG_FHA_1	456	462	PF00498	0.416
LIG_FHA_1	57	63	PF00498	0.614
LIG_FHA_1	7	13	PF00498	0.356
LIG_FHA_2	178	184	PF00498	0.705
LIG_FHA_2	202	208	PF00498	0.489
LIG_FHA_2	287	293	PF00498	0.760
LIG_LIR_Apic_2	156	162	PF02991	0.523
LIG_LIR_Apic_2	392	398	PF02991	0.382
LIG_LIR_Gen_1	144	154	PF02991	0.575
LIG_LIR_Gen_1	16	25	PF02991	0.471
LIG_LIR_Gen_1	349	359	PF02991	0.379
LIG_LIR_Nem_3	144	150	PF02991	0.552
LIG_LIR_Nem_3	16	21	PF02991	0.406
LIG_LIR_Nem_3	349	355	PF02991	0.359
LIG_LIR_Nem_3	486	491	PF02991	0.482
LIG_NRBOX	208	214	PF00104	0.430
LIG_Pex14_1	13	17	PF04695	0.339
LIG_Pex14_1	488	492	PF04695	0.500
LIG_Rb_pABgroove_1	346	354	PF01858	0.371
LIG_SH2_CRK	159	163	PF00017	0.525
LIG_SH2_CRK	464	468	PF00017	0.428
LIG_SH2_NCK_1	147	151	PF00017	0.523
LIG_SH2_NCK_1	159	163	PF00017	0.509
LIG_SH2_NCK_1	391	395	PF00017	0.453
LIG_SH2_SRC	325	328	PF00017	0.458
LIG_SH2_SRC	391	394	PF00017	0.452
LIG_SH2_STAP1	325	329	PF00017	0.516
LIG_SH2_STAT3	359	362	PF00017	0.423
LIG_SH2_STAT5	423	426	PF00017	0.329
LIG_SH3_3	235	241	PF00018	0.400
LIG_SH3_3	442	448	PF00018	0.364
LIG_SH3_3	97	103	PF00018	0.428
LIG_SUMO_SIM_par_1	375	380	PF11976	0.338
LIG_TRAF2_1	179	182	PF00917	0.634
LIG_TYR_ITIM	462	467	PF00017	0.414
LIG_WRC_WIRS_1	306	311	PF05994	0.427
MOD_CK1_1	127	133	PF00069	0.527
MOD_CK1_1	160	166	PF00069	0.642
MOD_CK1_1	224	230	PF00069	0.583
MOD_CK1_1	279	285	PF00069	0.696
MOD_CK1_1	286	292	PF00069	0.662
MOD_CK1_1	368	374	PF00069	0.537
MOD_CK1_1	396	402	PF00069	0.496
MOD_CK1_1	42	48	PF00069	0.705
MOD_CK1_1	481	487	PF00069	0.516
MOD_CK1_1	65	71	PF00069	0.714
MOD_CK2_1	148	154	PF00069	0.719
MOD_CK2_1	177	183	PF00069	0.653
MOD_CK2_1	201	207	PF00069	0.497
MOD_CK2_1	286	292	PF00069	0.787
MOD_CK2_1	74	80	PF00069	0.451
MOD_Cter_Amidation	312	315	PF01082	0.279
MOD_GlcNHglycan	126	129	PF01048	0.517
MOD_GlcNHglycan	226	229	PF01048	0.536
MOD_GlcNHglycan	281	284	PF01048	0.667
MOD_GlcNHglycan	296	299	PF01048	0.646
MOD_GlcNHglycan	311	314	PF01048	0.316
MOD_GlcNHglycan	432	435	PF01048	0.435
MOD_GlcNHglycan	480	483	PF01048	0.424
MOD_GSK3_1	123	130	PF00069	0.508
MOD_GSK3_1	153	160	PF00069	0.665
MOD_GSK3_1	177	184	PF00069	0.717
MOD_GSK3_1	224	231	PF00069	0.571
MOD_GSK3_1	265	272	PF00069	0.661
MOD_GSK3_1	279	286	PF00069	0.703
MOD_GSK3_1	294	301	PF00069	0.731
MOD_GSK3_1	305	312	PF00069	0.461
MOD_GSK3_1	361	368	PF00069	0.561
MOD_GSK3_1	396	403	PF00069	0.460
MOD_GSK3_1	62	69	PF00069	0.621
MOD_N-GLC_1	224	229	PF02516	0.659
MOD_NEK2_1	157	162	PF00069	0.598
MOD_NEK2_1	309	314	PF00069	0.368
MOD_NEK2_1	455	460	PF00069	0.414
MOD_NEK2_1	501	506	PF00069	0.513
MOD_NEK2_2	13	18	PF00069	0.370
MOD_NEK2_2	141	146	PF00069	0.607
MOD_NEK2_2	283	288	PF00069	0.524
MOD_NEK2_2	298	303	PF00069	0.522
MOD_PIKK_1	157	163	PF00454	0.652
MOD_PIKK_1	189	195	PF00454	0.619
MOD_PIKK_1	396	402	PF00454	0.545
MOD_PKA_2	177	183	PF00069	0.699
MOD_PKA_2	189	195	PF00069	0.544
MOD_PKA_2	279	285	PF00069	0.528
MOD_PKA_2	465	471	PF00069	0.413
MOD_PKA_2	501	507	PF00069	0.585
MOD_PKB_1	303	311	PF00069	0.451
MOD_Plk_1	153	159	PF00069	0.573
MOD_Plk_1	181	187	PF00069	0.630
MOD_Plk_4	181	187	PF00069	0.805
MOD_Plk_4	305	311	PF00069	0.429
MOD_Plk_4	400	406	PF00069	0.390
MOD_Plk_4	8	14	PF00069	0.427
MOD_ProDKin_1	226	232	PF00069	0.586
MOD_ProDKin_1	240	246	PF00069	0.324
MOD_ProDKin_1	365	371	PF00069	0.697
MOD_ProDKin_1	42	48	PF00069	0.681
MOD_ProDKin_1	49	55	PF00069	0.604
MOD_ProDKin_1	62	68	PF00069	0.667
MOD_SUMO_rev_2	77	83	PF00179	0.427
TRG_DiLeu_BaLyEn_6	208	213	PF01217	0.478
TRG_DiLeu_BaLyEn_6	229	234	PF01217	0.479
TRG_ENDOCYTIC_2	114	117	PF00928	0.481
TRG_ENDOCYTIC_2	147	150	PF00928	0.605
TRG_ENDOCYTIC_2	464	467	PF00928	0.425
TRG_ER_diArg_1	209	211	PF00400	0.484
TRG_ER_diArg_1	302	305	PF00400	0.499
TRG_ER_diArg_1	314	316	PF00400	0.440
TRG_ER_diArg_1	500	503	PF00400	0.477
TRG_ER_diArg_1	92	95	PF00400	0.464
TRG_Pf-PMV_PEXEL_1	210	215	PF00026	0.434
TRG_Pf-PMV_PEXEL_1	338	342	PF00026	0.465

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IIC5	Leptomonas seymouri	45%	98%
A0A3S7WSX9	Leishmania donovani	89%	100%
A4H7L7	Leishmania braziliensis	70%	100%
A4HW02	Leishmania infantum	89%	100%
Q4QFS5	Leishmania major	89%	100%
V5BIX5	Trypanosoma cruzi	30%	100%