Amastin surface glycofamily protein

Quick info Annotations Localization Expansion Sequence features Structure Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Amastin surface glycoprotein, putative

Species:

Leishmania mexicana

UniProt:

E9APP3_LEIMU

TriTrypDb:

LmxM.14.0490

Length:

276

Annotations

LeishMANIAdb annotations

Structurally similar to the amastin family proteins, but with a rather divergent sequence. Also seems to be related to mammalian clarin proteins.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 6
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 4

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	10
GO:0110165	cellular anatomical entity	1	10

Expansion

Sequence features

E9APP3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APP3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	112	114	PF00675	0.591
CLV_NRD_NRD_1	215	217	PF00675	0.772
CLV_NRD_NRD_1	22	24	PF00675	0.564
CLV_NRD_NRD_1	29	31	PF00675	0.540
CLV_NRD_NRD_1	37	39	PF00675	0.512
CLV_PCSK_KEX2_1	22	24	PF00082	0.564
CLV_PCSK_KEX2_1	29	31	PF00082	0.540
CLV_PCSK_KEX2_1	37	39	PF00082	0.512
CLV_PCSK_SKI1_1	129	133	PF00082	0.533
CLV_PCSK_SKI1_1	266	270	PF00082	0.480
CLV_PCSK_SKI1_1	37	41	PF00082	0.585
CLV_PCSK_SKI1_1	79	83	PF00082	0.521
DEG_Nend_UBRbox_1	1	4	PF02207	0.828
DOC_MAPK_gen_1	45	52	PF00069	0.561
DOC_MAPK_MEF2A_6	45	52	PF00069	0.561
DOC_PP2B_LxvP_1	40	43	PF13499	0.658
DOC_USP7_UBL2_3	217	221	PF12436	0.431
LIG_14-3-3_CanoR_1	22	28	PF00244	0.871
LIG_14-3-3_CanoR_1	257	262	PF00244	0.790
LIG_APCC_ABBA_1	198	203	PF00400	0.327
LIG_APCC_ABBA_1	9	14	PF00400	0.652
LIG_BRCT_BRCA1_1	101	105	PF00533	0.409
LIG_BRCT_BRCA1_1	226	230	PF00533	0.479
LIG_eIF4E_1	44	50	PF01652	0.472
LIG_FHA_1	141	147	PF00498	0.400
LIG_FHA_1	165	171	PF00498	0.452
LIG_FHA_1	187	193	PF00498	0.429
LIG_FHA_1	195	201	PF00498	0.446
LIG_FHA_1	29	35	PF00498	0.641
LIG_FHA_1	53	59	PF00498	0.492
LIG_FHA_1	68	74	PF00498	0.405
LIG_FHA_1	76	82	PF00498	0.399
LIG_LIR_Gen_1	10	18	PF02991	0.652
LIG_LIR_Gen_1	141	150	PF02991	0.366
LIG_LIR_Gen_1	159	168	PF02991	0.252
LIG_LIR_Gen_1	174	181	PF02991	0.487
LIG_LIR_Gen_1	183	192	PF02991	0.422
LIG_LIR_Gen_1	222	231	PF02991	0.406
LIG_LIR_Gen_1	247	258	PF02991	0.526
LIG_LIR_Nem_3	10	15	PF02991	0.652
LIG_LIR_Nem_3	159	163	PF02991	0.450
LIG_LIR_Nem_3	174	178	PF02991	0.487
LIG_LIR_Nem_3	183	187	PF02991	0.422
LIG_LIR_Nem_3	222	226	PF02991	0.398
LIG_LIR_Nem_3	247	253	PF02991	0.377
LIG_Pex14_2	226	230	PF04695	0.411
LIG_Pex14_2	242	246	PF04695	0.409
LIG_PTB_Apo_2	130	137	PF02174	0.398
LIG_SH2_CRK	158	162	PF00017	0.409
LIG_SH2_CRK	175	179	PF00017	0.561
LIG_SH2_CRK	250	254	PF00017	0.490
LIG_SH2_SRC	201	204	PF00017	0.357
LIG_SH2_STAP1	121	125	PF00017	0.390
LIG_SH2_STAP1	142	146	PF00017	0.463
LIG_SH2_STAP1	175	179	PF00017	0.504
LIG_SH2_STAP1	201	205	PF00017	0.441
LIG_SH2_STAP1	250	254	PF00017	0.600
LIG_SH2_STAT5	121	124	PF00017	0.439
LIG_SH2_STAT5	126	129	PF00017	0.414
LIG_SH2_STAT5	142	145	PF00017	0.402
LIG_SH2_STAT5	175	178	PF00017	0.515
LIG_SH2_STAT5	223	226	PF00017	0.397
LIG_SH2_STAT5	250	253	PF00017	0.518
LIG_SH2_STAT5	41	44	PF00017	0.661
LIG_SUMO_SIM_par_1	188	193	PF11976	0.565
LIG_SUMO_SIM_par_1	70	76	PF11976	0.339
LIG_SxIP_EBH_1	206	219	PF03271	0.355
LIG_TYR_ITIM	248	253	PF00017	0.390
MOD_CK1_1	119	125	PF00069	0.528
MOD_CK1_1	138	144	PF00069	0.386
MOD_CK1_1	17	23	PF00069	0.775
MOD_CK1_1	204	210	PF00069	0.567
MOD_CK2_1	94	100	PF00069	0.536
MOD_Cter_Amidation	214	217	PF01082	0.776
MOD_Cter_Amidation	35	38	PF01082	0.440
MOD_GlcNHglycan	137	140	PF01048	0.529
MOD_GSK3_1	10	17	PF00069	0.756
MOD_GSK3_1	115	122	PF00069	0.415
MOD_GSK3_1	18	25	PF00069	0.763
MOD_GSK3_1	182	189	PF00069	0.402
MOD_GSK3_1	190	197	PF00069	0.421
MOD_GSK3_1	204	211	PF00069	0.390
MOD_GSK3_1	244	251	PF00069	0.549
MOD_N-GLC_1	208	213	PF02516	0.756
MOD_NEK2_1	105	110	PF00069	0.339
MOD_NEK2_1	18	23	PF00069	0.875
MOD_NEK2_1	208	213	PF00069	0.548
MOD_NEK2_1	226	231	PF00069	0.314
MOD_NEK2_1	232	237	PF00069	0.409
MOD_NEK2_1	244	249	PF00069	0.409
MOD_NEK2_1	52	57	PF00069	0.492
MOD_NEK2_1	94	99	PF00069	0.468
MOD_NEK2_2	116	121	PF00069	0.548
MOD_PKA_1	22	28	PF00069	0.757
MOD_PKA_1	29	35	PF00069	0.731
MOD_PKA_2	18	24	PF00069	0.762
MOD_PKA_2	28	34	PF00069	0.733
MOD_Plk_1	13	19	PF00069	0.760
MOD_Plk_1	173	179	PF00069	0.561
MOD_Plk_1	208	214	PF00069	0.561
MOD_Plk_4	105	111	PF00069	0.384
MOD_Plk_4	116	122	PF00069	0.436
MOD_Plk_4	140	146	PF00069	0.392
MOD_Plk_4	156	162	PF00069	0.471
MOD_Plk_4	186	192	PF00069	0.347
MOD_Plk_4	257	263	PF00069	0.749
MOD_SUMO_rev_2	260	268	PF00179	0.703
TRG_ENDOCYTIC_2	142	145	PF00928	0.479
TRG_ENDOCYTIC_2	158	161	PF00928	0.334
TRG_ENDOCYTIC_2	175	178	PF00928	0.561
TRG_ENDOCYTIC_2	223	226	PF00928	0.397
TRG_ENDOCYTIC_2	250	253	PF00928	0.518
TRG_ER_diArg_1	22	24	PF00400	0.878
TRG_ER_diArg_1	37	39	PF00400	0.649
TRG_NES_CRM1_1	86	100	PF08389	0.533
TRG_NLS_MonoExtN_4	214	220	PF00514	0.348

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P2B8	Leptomonas seymouri	67%	99%
A0A3S5H6R5	Leishmania donovani	84%	100%
A0A3S7WSV8	Leishmania donovani	84%	100%
A4H7K8	Leishmania braziliensis	72%	100%
A4H7K9	Leishmania braziliensis	72%	100%
A4HVZ0	Leishmania infantum	84%	100%
A4HVZ1	Leishmania infantum	85%	100%
E9APP2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	99%	100%
Q4QFT6	Leishmania major	86%	100%
Q4QFT7	Leishmania major	84%	100%