Na_Ca_ex domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania mexicana

UniProt:

E9APM0_LEIMU

TriTrypDb:

LmxM.14.0270

Length:

494

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005774	vacuolar membrane	5	7
GO:0016020	membrane	2	8
GO:0031090	organelle membrane	3	7
GO:0098588	bounding membrane of organelle	4	7
GO:0110165	cellular anatomical entity	1	8

Expansion

Sequence features

E9APM0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APM0

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	1
GO:0006811	monoatomic ion transport	4	1
GO:0006812	monoatomic cation transport	5	1
GO:0006816	calcium ion transport	7	1
GO:0006873	intracellular monoatomic ion homeostasis	4	1
GO:0006874	intracellular calcium ion homeostasis	7	1
GO:0006875	obsolete intracellular metal ion homeostasis	6	1
GO:0009987	cellular process	1	1
GO:0019725	cellular homeostasis	2	1
GO:0030001	metal ion transport	6	1
GO:0030003	intracellular monoatomic cation homeostasis	5	1
GO:0034220	monoatomic ion transmembrane transport	3	1
GO:0042592	homeostatic process	3	1
GO:0048878	chemical homeostasis	4	1
GO:0050801	monoatomic ion homeostasis	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0055065	obsolete metal ion homeostasis	7	1
GO:0055074	calcium ion homeostasis	8	1
GO:0055080	monoatomic cation homeostasis	6	1
GO:0055082	intracellular chemical homeostasis	3	1
GO:0055085	transmembrane transport	2	1
GO:0065007	biological regulation	1	1
GO:0065008	regulation of biological quality	2	1
GO:0070588	calcium ion transmembrane transport	6	1
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	1
GO:0072507	obsolete divalent inorganic cation homeostasis	7	1
GO:0098655	monoatomic cation transmembrane transport	4	1
GO:0098660	inorganic ion transmembrane transport	4	1
GO:0098662	inorganic cation transmembrane transport	5	1
GO:0098771	inorganic ion homeostasis	6	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	8
GO:0005451	obsolete monoatomic cation:proton antiporter activity	5	4
GO:0008324	monoatomic cation transmembrane transporter activity	4	8
GO:0015075	monoatomic ion transmembrane transporter activity	3	8
GO:0015078	proton transmembrane transporter activity	5	4
GO:0015085	calcium ion transmembrane transporter activity	6	4
GO:0015291	secondary active transmembrane transporter activity	4	4
GO:0015297	antiporter activity	5	4
GO:0015298	obsolete solute:monoatomic cation antiporter activity	5	4
GO:0015299	obsolete solute:proton antiporter activity	6	4
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	4
GO:0015368	calcium:monoatomic cation antiporter activity	7	4
GO:0015369	calcium:proton antiporter activity	8	4
GO:0022804	active transmembrane transporter activity	3	4
GO:0022853	active monoatomic ion transmembrane transporter activity	4	4
GO:0022857	transmembrane transporter activity	2	8
GO:0022890	inorganic cation transmembrane transporter activity	4	4
GO:0046873	metal ion transmembrane transporter activity	5	4
GO:0051139	metal cation:proton antiporter activity	7	4
GO:0140828	metal cation:monoatomic cation antiporter activity	6	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	35	39	PF00656	0.765
CLV_C14_Caspase3-7	92	96	PF00656	0.522
CLV_NRD_NRD_1	249	251	PF00675	0.566
CLV_PCSK_KEX2_1	249	251	PF00082	0.566
CLV_PCSK_SKI1_1	177	181	PF00082	0.496
CLV_PCSK_SKI1_1	361	365	PF00082	0.551
CLV_PCSK_SKI1_1	74	78	PF00082	0.388
DEG_Nend_Nbox_1	1	3	PF02207	0.747
DOC_CDC14_PxL_1	413	421	PF14671	0.459
DOC_CKS1_1	251	256	PF01111	0.259
DOC_CYCLIN_yCln2_LP_2	427	433	PF00134	0.338
DOC_CYCLIN_yCln2_LP_2	79	85	PF00134	0.386
DOC_MAPK_gen_1	317	326	PF00069	0.599
DOC_MAPK_MEF2A_6	139	147	PF00069	0.496
DOC_MAPK_MEF2A_6	361	370	PF00069	0.331
DOC_MAPK_MEF2A_6	74	83	PF00069	0.326
DOC_PP1_RVXF_1	456	462	PF00149	0.591
DOC_PP2B_LxvP_1	121	124	PF13499	0.409
DOC_PP2B_LxvP_1	197	200	PF13499	0.409
DOC_PP2B_LxvP_1	427	430	PF13499	0.384
DOC_PP2B_LxvP_1	79	82	PF13499	0.386
DOC_PP4_FxxP_1	229	232	PF00568	0.328
DOC_PP4_FxxP_1	414	417	PF00568	0.361
DOC_SPAK_OSR1_1	333	337	PF12202	0.459
DOC_USP7_MATH_1	385	389	PF00917	0.544
DOC_USP7_MATH_1	404	408	PF00917	0.170
DOC_USP7_MATH_1	489	493	PF00917	0.519
DOC_USP7_MATH_1	94	98	PF00917	0.512
DOC_WW_Pin1_4	139	144	PF00397	0.566
DOC_WW_Pin1_4	250	255	PF00397	0.309
DOC_WW_Pin1_4	298	303	PF00397	0.657
DOC_WW_Pin1_4	36	41	PF00397	0.744
LIG_14-3-3_CanoR_1	17	23	PF00244	0.751
LIG_14-3-3_CanoR_1	329	336	PF00244	0.631
LIG_14-3-3_CanoR_1	33	42	PF00244	0.762
LIG_Actin_WH2_2	305	321	PF00022	0.649
LIG_BRCT_BRCA1_1	330	334	PF00533	0.567
LIG_BRCT_BRCA1_1	4	8	PF00533	0.661
LIG_CaM_IQ_9	309	324	PF13499	0.602
LIG_CSL_BTD_1	79	82	PF09270	0.434
LIG_CtBP_PxDLS_1	430	434	PF00389	0.363
LIG_FHA_1	123	129	PF00498	0.386
LIG_FHA_1	21	27	PF00498	0.751
LIG_FHA_1	319	325	PF00498	0.670
LIG_FHA_1	335	341	PF00498	0.222
LIG_FHA_1	369	375	PF00498	0.416
LIG_FHA_1	404	410	PF00498	0.478
LIG_FHA_1	445	451	PF00498	0.432
LIG_FHA_2	203	209	PF00498	0.515
LIG_IRF3_LxIS_1	207	212	PF10401	0.531
LIG_IRF3_LxIS_1	263	270	PF10401	0.169
LIG_LIR_Apic_2	172	178	PF02991	0.331
LIG_LIR_Apic_2	226	232	PF02991	0.304
LIG_LIR_Gen_1	158	167	PF02991	0.422
LIG_LIR_Gen_1	212	222	PF02991	0.531
LIG_LIR_LC3C_4	446	450	PF02991	0.459
LIG_LIR_Nem_3	158	164	PF02991	0.378
LIG_LIR_Nem_3	212	217	PF02991	0.474
LIG_LIR_Nem_3	432	438	PF02991	0.301
LIG_LIR_Nem_3	66	72	PF02991	0.692
LIG_NRBOX	184	190	PF00104	0.459
LIG_PCNA_yPIPBox_3	126	139	PF02747	0.346
LIG_PTB_Apo_2	262	269	PF02174	0.205
LIG_SH2_CRK	412	416	PF00017	0.459
LIG_SH2_CRK	471	475	PF00017	0.361
LIG_SH2_STAP1	281	285	PF00017	0.302
LIG_SH2_STAP1	310	314	PF00017	0.598
LIG_SH2_STAP1	330	334	PF00017	0.567
LIG_SH2_STAP1	471	475	PF00017	0.409
LIG_SH2_STAT3	310	313	PF00017	0.563
LIG_SH2_STAT5	132	135	PF00017	0.531
LIG_SH2_STAT5	284	287	PF00017	0.271
LIG_SH2_STAT5	296	299	PF00017	0.615
LIG_SH2_STAT5	72	75	PF00017	0.470
LIG_SH3_3	236	242	PF00018	0.409
LIG_SH3_3	299	305	PF00018	0.658
LIG_SH3_3	363	369	PF00018	0.296
LIG_SH3_3	99	105	PF00018	0.517
LIG_SUMO_SIM_anti_2	371	377	PF11976	0.377
LIG_SUMO_SIM_par_1	119	125	PF11976	0.350
LIG_SUMO_SIM_par_1	126	131	PF11976	0.301
LIG_SUMO_SIM_par_1	350	356	PF11976	0.434
LIG_SUMO_SIM_par_1	429	434	PF11976	0.406
LIG_SUMO_SIM_par_1	82	88	PF11976	0.290
LIG_UBA3_1	129	134	PF00899	0.184
MOD_CDK_SPxK_1	250	256	PF00069	0.259
MOD_CK1_1	169	175	PF00069	0.284
MOD_CK1_1	20	26	PF00069	0.700
MOD_CK1_1	212	218	PF00069	0.476
MOD_CK1_1	27	33	PF00069	0.682
MOD_CK1_1	298	304	PF00069	0.698
MOD_CK1_1	328	334	PF00069	0.608
MOD_CK1_1	36	42	PF00069	0.753
MOD_GlcNHglycan	217	220	PF01048	0.298
MOD_GlcNHglycan	26	29	PF01048	0.516
MOD_GlcNHglycan	305	308	PF01048	0.260
MOD_GlcNHglycan	35	38	PF01048	0.532
MOD_GlcNHglycan	383	386	PF01048	0.312
MOD_GlcNHglycan	4	7	PF01048	0.510
MOD_GlcNHglycan	98	101	PF01048	0.741
MOD_GSK3_1	13	20	PF00069	0.720
MOD_GSK3_1	32	39	PF00069	0.768
MOD_GSK3_1	381	388	PF00069	0.442
MOD_GSK3_1	85	92	PF00069	0.390
MOD_NEK2_1	159	164	PF00069	0.328
MOD_NEK2_1	166	171	PF00069	0.272
MOD_NEK2_1	181	186	PF00069	0.236
MOD_NEK2_1	192	197	PF00069	0.353
MOD_NEK2_1	2	7	PF00069	0.736
MOD_NEK2_1	209	214	PF00069	0.459
MOD_NEK2_1	217	222	PF00069	0.462
MOD_NEK2_1	267	272	PF00069	0.328
MOD_NEK2_1	32	37	PF00069	0.735
MOD_NEK2_1	334	339	PF00069	0.227
MOD_NEK2_1	341	346	PF00069	0.277
MOD_NEK2_1	353	358	PF00069	0.427
MOD_NEK2_1	362	367	PF00069	0.442
MOD_NEK2_1	431	436	PF00069	0.363
MOD_NEK2_1	444	449	PF00069	0.323
MOD_NEK2_1	63	68	PF00069	0.669
MOD_NEK2_1	85	90	PF00069	0.270
MOD_PIKK_1	328	334	PF00454	0.639
MOD_PIKK_1	389	395	PF00454	0.571
MOD_PKA_2	318	324	PF00069	0.662
MOD_PKA_2	32	38	PF00069	0.763
MOD_PKA_2	328	334	PF00069	0.520
MOD_Plk_1	431	437	PF00069	0.366
MOD_Plk_2-3	203	209	PF00069	0.531
MOD_Plk_4	124	130	PF00069	0.381
MOD_Plk_4	181	187	PF00069	0.340
MOD_Plk_4	192	198	PF00069	0.310
MOD_Plk_4	223	229	PF00069	0.348
MOD_Plk_4	230	236	PF00069	0.293
MOD_Plk_4	27	33	PF00069	0.734
MOD_Plk_4	336	342	PF00069	0.388
MOD_Plk_4	385	391	PF00069	0.535
MOD_Plk_4	404	410	PF00069	0.409
MOD_Plk_4	444	450	PF00069	0.409
MOD_Plk_4	463	469	PF00069	0.527
MOD_ProDKin_1	139	145	PF00069	0.566
MOD_ProDKin_1	250	256	PF00069	0.309
MOD_ProDKin_1	298	304	PF00069	0.654
MOD_ProDKin_1	36	42	PF00069	0.745
TRG_DiLeu_BaEn_1	463	468	PF01217	0.496
TRG_ENDOCYTIC_2	412	415	PF00928	0.409
TRG_ENDOCYTIC_2	471	474	PF00928	0.328
TRG_ER_diArg_1	248	250	PF00400	0.366
TRG_ER_diArg_1	458	461	PF00400	0.584

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HT55	Leptomonas seymouri	62%	100%
A0A0S4J0Y4	Bodo saltans	27%	100%
A0A1X0NNQ4	Trypanosomatidae	33%	100%
A0A3Q8ID24	Leishmania donovani	91%	100%
A4HVW9	Leishmania infantum	91%	100%
O59768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	25%	100%
P74072	Synechocystis sp. (strain PCC 6803 / Kazusa)	27%	100%
Q39253	Arabidopsis thaliana	23%	100%
Q39254	Arabidopsis thaliana	24%	100%
Q4QFV8	Leishmania major	89%	100%
Q5KQN0	Oryza sativa subsp. japonica	23%	100%
Q6K1C4	Oryza sativa subsp. japonica	23%	100%
Q6YXZ1	Oryza sativa subsp. japonica	26%	100%
Q75XW3	Aphanothece halophytica	27%	100%
Q8L783	Arabidopsis thaliana	23%	100%
Q945S5	Arabidopsis thaliana	24%	100%