Phosphoprotein phosphatase-like protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

phosphoprotein phosphatase-like protein

Species:

Leishmania mexicana

UniProt:

E9API1_LEIMU

TriTrypDb:

LmxM.13.1570

Length:

588

Annotations

LeishMANIAdb annotations

A large collection of various protein phosphatases. Very highly expanded in kinetoplastids.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0005955	calcineurin complex	3	1
GO:0008287	protein serine/threonine phosphatase complex	4	1
GO:0032991	protein-containing complex	1	1
GO:0110165	cellular anatomical entity	1	3
GO:0140535	intracellular protein-containing complex	2	1
GO:1902494	catalytic complex	2	1
GO:1903293	phosphatase complex	3	1
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2

Expansion

Sequence features

E9API1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9API1

Function

Biological processes
Term	Name	Level	Count
GO:0007165	signal transduction	2	5
GO:0009987	cellular process	1	7
GO:0019722	calcium-mediated signaling	5	5
GO:0019932	second-messenger-mediated signaling	4	5
GO:0035556	intracellular signal transduction	3	5
GO:0050789	regulation of biological process	2	5
GO:0050794	regulation of cellular process	3	5
GO:0065007	biological regulation	1	5
GO:0097720	calcineurin-mediated signaling	6	5
GO:0000724	double-strand break repair via homologous recombination	7	2
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006302	double-strand break repair	6	2
GO:0006310	DNA recombination	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	2
GO:0006974	DNA damage response	4	2
GO:0008152	metabolic process	1	2
GO:0033554	cellular response to stress	3	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0050896	response to stimulus	1	2
GO:0051716	cellular response to stimulus	2	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	16
GO:0004721	phosphoprotein phosphatase activity	3	16
GO:0004722	protein serine/threonine phosphatase activity	4	16
GO:0016787	hydrolase activity	2	16
GO:0016788	hydrolase activity, acting on ester bonds	3	16
GO:0016791	phosphatase activity	5	16
GO:0017018	myosin phosphatase activity	5	16
GO:0042578	phosphoric ester hydrolase activity	4	16
GO:0140096	catalytic activity, acting on a protein	2	16
GO:0004723	calcium-dependent protein serine/threonine phosphatase activity	5	5
GO:0005488	binding	1	1
GO:0005515	protein binding	2	1
GO:0005516	calmodulin binding	3	1
GO:0033192	calmodulin-dependent protein phosphatase activity	6	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	155	159	PF00656	0.498
CLV_C14_Caspase3-7	261	265	PF00656	0.557
CLV_NRD_NRD_1	248	250	PF00675	0.393
CLV_NRD_NRD_1	332	334	PF00675	0.323
CLV_PCSK_KEX2_1	227	229	PF00082	0.476
CLV_PCSK_KEX2_1	248	250	PF00082	0.393
CLV_PCSK_KEX2_1	331	333	PF00082	0.322
CLV_PCSK_PC1ET2_1	227	229	PF00082	0.476
CLV_PCSK_SKI1_1	227	231	PF00082	0.433
CLV_PCSK_SKI1_1	342	346	PF00082	0.392
CLV_PCSK_SKI1_1	414	418	PF00082	0.370
CLV_PCSK_SKI1_1	531	535	PF00082	0.313
CLV_PCSK_SKI1_1	64	68	PF00082	0.563
DEG_Nend_UBRbox_2	1	3	PF02207	0.541
DEG_SPOP_SBC_1	129	133	PF00917	0.510
DEG_SPOP_SBC_1	41	45	PF00917	0.525
DOC_ANK_TNKS_1	332	339	PF00023	0.309
DOC_CYCLIN_yCln2_LP_2	417	423	PF00134	0.334
DOC_MAPK_MEF2A_6	352	361	PF00069	0.322
DOC_MAPK_MEF2A_6	414	421	PF00069	0.334
DOC_MAPK_NFAT4_5	414	422	PF00069	0.334
DOC_PP2B_LxvP_1	109	112	PF13499	0.531
DOC_PP2B_LxvP_1	417	420	PF13499	0.334
DOC_PP4_FxxP_1	230	233	PF00568	0.328
DOC_PP4_FxxP_1	536	539	PF00568	0.460
DOC_SPAK_OSR1_1	431	435	PF12202	0.334
DOC_USP7_MATH_1	103	107	PF00917	0.629
DOC_USP7_MATH_1	112	116	PF00917	0.509
DOC_USP7_MATH_1	127	131	PF00917	0.555
DOC_USP7_MATH_1	215	219	PF00917	0.564
DOC_USP7_MATH_1	498	502	PF00917	0.301
DOC_WW_Pin1_4	101	106	PF00397	0.484
DOC_WW_Pin1_4	123	128	PF00397	0.596
DOC_WW_Pin1_4	175	180	PF00397	0.557
DOC_WW_Pin1_4	184	189	PF00397	0.575
DOC_WW_Pin1_4	191	196	PF00397	0.556
DOC_WW_Pin1_4	437	442	PF00397	0.506
DOC_WW_Pin1_4	56	61	PF00397	0.544
DOC_WW_Pin1_4	75	80	PF00397	0.513
DOC_WW_Pin1_4	83	88	PF00397	0.546
LIG_14-3-3_CanoR_1	196	206	PF00244	0.656
LIG_14-3-3_CanoR_1	352	358	PF00244	0.334
LIG_14-3-3_CanoR_1	431	441	PF00244	0.334
LIG_14-3-3_CanoR_1	454	462	PF00244	0.254
LIG_14-3-3_CanoR_1	531	536	PF00244	0.320
LIG_14-3-3_CanoR_1	64	73	PF00244	0.525
LIG_Actin_WH2_2	203	219	PF00022	0.509
LIG_BRCT_BRCA1_1	235	239	PF00533	0.413
LIG_BRCT_BRCA1_1	3	7	PF00533	0.511
LIG_BRCT_BRCA1_1	500	504	PF00533	0.397
LIG_BRCT_BRCA1_1	85	89	PF00533	0.497
LIG_BRCT_MDC1_1	584	588	PF00533	0.436
LIG_Clathr_ClatBox_1	296	300	PF01394	0.367
LIG_FAT_LD_1	357	365	PF03623	0.242
LIG_FHA_1	285	291	PF00498	0.464
LIG_FHA_1	304	310	PF00498	0.377
LIG_FHA_1	32	38	PF00498	0.542
LIG_FHA_1	320	326	PF00498	0.274
LIG_FHA_1	384	390	PF00498	0.334
LIG_FHA_1	395	401	PF00498	0.225
LIG_FHA_1	469	475	PF00498	0.271
LIG_FHA_1	65	71	PF00498	0.559
LIG_FHA_2	203	209	PF00498	0.581
LIG_FHA_2	461	467	PF00498	0.254
LIG_FHA_2	525	531	PF00498	0.393
LIG_Integrin_RGD_1	252	254	PF01839	0.435
LIG_IRF3_LxIS_1	195	200	PF10401	0.546
LIG_LIR_Apic_2	534	539	PF02991	0.457
LIG_LIR_Gen_1	319	329	PF02991	0.305
LIG_LIR_Gen_1	340	350	PF02991	0.437
LIG_LIR_Gen_1	406	417	PF02991	0.365
LIG_LIR_Gen_1	427	434	PF02991	0.394
LIG_LIR_Gen_1	507	516	PF02991	0.299
LIG_LIR_LC3C_4	305	309	PF02991	0.334
LIG_LIR_Nem_3	236	242	PF02991	0.389
LIG_LIR_Nem_3	319	324	PF02991	0.305
LIG_LIR_Nem_3	369	373	PF02991	0.334
LIG_LIR_Nem_3	387	393	PF02991	0.267
LIG_LIR_Nem_3	406	412	PF02991	0.349
LIG_LIR_Nem_3	427	432	PF02991	0.394
LIG_LIR_Nem_3	507	513	PF02991	0.332
LIG_LIR_Nem_3	562	566	PF02991	0.414
LIG_LIR_Nem_3	571	577	PF02991	0.462
LIG_LIR_Nem_3	86	92	PF02991	0.493
LIG_LYPXL_yS_3	370	373	PF13949	0.334
LIG_MAD2	531	539	PF02301	0.347
LIG_PCNA_PIPBox_1	518	527	PF02747	0.344
LIG_PCNA_yPIPBox_3	515	525	PF02747	0.346
LIG_PTB_Apo_2	272	279	PF02174	0.377
LIG_PTB_Phospho_1	272	278	PF10480	0.385
LIG_SH2_CRK	321	325	PF00017	0.402
LIG_SH2_CRK	390	394	PF00017	0.334
LIG_SH2_CRK	541	545	PF00017	0.399
LIG_SH2_STAP1	321	325	PF00017	0.334
LIG_SH2_STAT5	278	281	PF00017	0.422
LIG_SH2_STAT5	321	324	PF00017	0.324
LIG_SH2_STAT5	349	352	PF00017	0.345
LIG_SH2_STAT5	375	378	PF00017	0.334
LIG_SH2_STAT5	524	527	PF00017	0.347
LIG_SH2_STAT5	541	544	PF00017	0.496
LIG_SH2_STAT5	73	76	PF00017	0.579
LIG_SH3_3	121	127	PF00018	0.553
LIG_SH3_3	178	184	PF00018	0.539
LIG_SH3_3	23	29	PF00018	0.533
LIG_SH3_3	288	294	PF00018	0.348
LIG_SH3_3	365	371	PF00018	0.337
LIG_SH3_3	84	90	PF00018	0.524
LIG_SUMO_SIM_anti_2	298	303	PF11976	0.470
LIG_SUMO_SIM_anti_2	305	312	PF11976	0.389
LIG_SUMO_SIM_anti_2	356	361	PF11976	0.283
LIG_SUMO_SIM_par_1	287	293	PF11976	0.447
LIG_SUMO_SIM_par_1	295	300	PF11976	0.488
LIG_SUMO_SIM_par_1	305	312	PF11976	0.312
LIG_SUMO_SIM_par_1	33	40	PF11976	0.499
LIG_SUMO_SIM_par_1	530	537	PF11976	0.377
LIG_TRAF2_1	116	119	PF00917	0.556
LIG_TRAF2_1	445	448	PF00917	0.217
LIG_TYR_ITIM	368	373	PF00017	0.334
LIG_UBA3_1	166	175	PF00899	0.506
LIG_UBA3_1	360	367	PF00899	0.339
LIG_WW_3	232	236	PF00397	0.322
MOD_CDK_SPK_2	191	196	PF00069	0.502
MOD_CDK_SPxxK_3	191	198	PF00069	0.550
MOD_CK1_1	130	136	PF00069	0.547
MOD_CK1_1	142	148	PF00069	0.524
MOD_CK1_1	159	165	PF00069	0.494
MOD_CK1_1	200	206	PF00069	0.606
MOD_CK1_1	40	46	PF00069	0.570
MOD_CK1_1	476	482	PF00069	0.350
MOD_CK1_1	51	57	PF00069	0.526
MOD_CK1_1	576	582	PF00069	0.415
MOD_CK2_1	215	221	PF00069	0.524
MOD_CK2_1	442	448	PF00069	0.227
MOD_CK2_1	460	466	PF00069	0.273
MOD_GlcNHglycan	105	108	PF01048	0.551
MOD_GlcNHglycan	114	117	PF01048	0.513
MOD_GlcNHglycan	132	135	PF01048	0.555
MOD_GlcNHglycan	223	226	PF01048	0.628
MOD_GlcNHglycan	235	238	PF01048	0.389
MOD_GlcNHglycan	39	42	PF01048	0.548
MOD_GlcNHglycan	409	412	PF01048	0.359
MOD_GlcNHglycan	458	461	PF01048	0.217
MOD_GlcNHglycan	498	501	PF01048	0.322
MOD_GlcNHglycan	536	539	PF01048	0.460
MOD_GlcNHglycan	554	557	PF01048	0.378
MOD_GSK3_1	123	130	PF00069	0.546
MOD_GSK3_1	138	145	PF00069	0.561
MOD_GSK3_1	156	163	PF00069	0.520
MOD_GSK3_1	197	204	PF00069	0.582
MOD_GSK3_1	217	224	PF00069	0.590
MOD_GSK3_1	258	265	PF00069	0.512
MOD_GSK3_1	31	38	PF00069	0.551
MOD_GSK3_1	391	398	PF00069	0.425
MOD_GSK3_1	403	410	PF00069	0.313
MOD_GSK3_1	456	463	PF00069	0.402
MOD_GSK3_1	51	58	PF00069	0.563
MOD_GSK3_1	91	98	PF00069	0.547
MOD_LATS_1	137	143	PF00433	0.507
MOD_N-GLC_1	145	150	PF02516	0.534
MOD_N-GLC_1	455	460	PF02516	0.254
MOD_N-GLC_1	56	61	PF02516	0.513
MOD_N-GLC_2	540	542	PF02516	0.491
MOD_NEK2_1	1	6	PF00069	0.562
MOD_NEK2_1	128	133	PF00069	0.556
MOD_NEK2_1	138	143	PF00069	0.585
MOD_NEK2_1	258	263	PF00069	0.470
MOD_NEK2_1	320	325	PF00069	0.333
MOD_NEK2_1	35	40	PF00069	0.523
MOD_NEK2_1	432	437	PF00069	0.334
MOD_NEK2_1	582	587	PF00069	0.507
MOD_NEK2_1	7	12	PF00069	0.555
MOD_PIKK_1	258	264	PF00454	0.580
MOD_PIKK_1	266	272	PF00454	0.456
MOD_PKA_2	142	148	PF00069	0.493
MOD_PKA_2	197	203	PF00069	0.643
MOD_PKA_2	216	222	PF00069	0.526
MOD_PKA_2	41	47	PF00069	0.566
MOD_Plk_1	1	7	PF00069	0.541
MOD_Plk_2-3	448	454	PF00069	0.273
MOD_Plk_2-3	573	579	PF00069	0.405
MOD_Plk_4	269	275	PF00069	0.559
MOD_Plk_4	285	291	PF00069	0.367
MOD_Plk_4	303	309	PF00069	0.460
MOD_Plk_4	320	326	PF00069	0.215
MOD_Plk_4	369	375	PF00069	0.335
MOD_Plk_4	424	430	PF00069	0.379
MOD_Plk_4	531	537	PF00069	0.320
MOD_Plk_4	582	588	PF00069	0.492
MOD_ProDKin_1	101	107	PF00069	0.485
MOD_ProDKin_1	123	129	PF00069	0.594
MOD_ProDKin_1	175	181	PF00069	0.558
MOD_ProDKin_1	184	190	PF00069	0.574
MOD_ProDKin_1	191	197	PF00069	0.555
MOD_ProDKin_1	437	443	PF00069	0.506
MOD_ProDKin_1	56	62	PF00069	0.544
MOD_ProDKin_1	75	81	PF00069	0.513
MOD_ProDKin_1	83	89	PF00069	0.548
MOD_SUMO_for_1	366	369	PF00179	0.334
MOD_SUMO_rev_2	382	387	PF00179	0.403
TRG_DiLeu_BaEn_1	292	297	PF01217	0.285
TRG_DiLeu_BaEn_1	358	363	PF01217	0.320
TRG_DiLeu_BaEn_1	369	374	PF01217	0.270
TRG_DiLeu_BaEn_4	292	298	PF01217	0.280
TRG_DiLeu_BaLyEn_6	238	243	PF01217	0.276
TRG_ENDOCYTIC_2	321	324	PF00928	0.418
TRG_ENDOCYTIC_2	370	373	PF00928	0.334
TRG_ENDOCYTIC_2	390	393	PF00928	0.334
TRG_ENDOCYTIC_2	429	432	PF00928	0.322
TRG_ENDOCYTIC_2	541	544	PF00928	0.436
TRG_ER_diArg_1	195	198	PF00400	0.529
TRG_ER_diArg_1	247	249	PF00400	0.397
TRG_ER_diArg_1	331	333	PF00400	0.322
TRG_NES_CRM1_1	358	369	PF08389	0.233
TRG_Pf-PMV_PEXEL_1	64	68	PF00026	0.509

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IM33	Bodo saltans	28%	77%
A0A3Q8I8M6	Leishmania donovani	81%	100%
A0A3Q8II54	Leishmania donovani	81%	100%
A0A3S7XAY3	Leishmania donovani	31%	100%
A4H7E7	Leishmania braziliensis	62%	99%
A4HP65	Leishmania braziliensis	32%	100%
A4HVT0	Leishmania infantum	81%	100%
A4HVT6	Leishmania infantum	80%	100%
A4IDH0	Leishmania infantum	31%	100%
E9APH5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	81%	100%
E9ASX3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
Q4Q1M5	Leishmania major	31%	100%
Q4QFZ7	Leishmania major	80%	100%
Q4QG03	Leishmania major	82%	100%