Putative ras-family member, GTP-binding protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ras-family member, GTP-binding protein, putative

Species:

Leishmania mexicana

UniProt:

E9API0_LEIMU

TriTrypDb:

LmxM.13.1560

Length:

361

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000323	lytic vacuole	6	1
GO:0005634	nucleus	5	1
GO:0005764	lysosome	7	1
GO:0005773	vacuole	5	1
GO:0032991	protein-containing complex	1	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1
GO:1902494	catalytic complex	2	1
GO:1905360	GTPase complex	3	1
GO:1990131	Gtr1-Gtr2 GTPase complex	4	1

Expansion

Sequence features

E9API0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9API0

Function

Biological processes
Term	Name	Level	Count
GO:0001101	response to acid chemical	3	1
GO:0006950	response to stress	2	1
GO:0007154	cell communication	2	1
GO:0009267	cellular response to starvation	4	1
GO:0009605	response to external stimulus	2	1
GO:0009719	response to endogenous stimulus	2	1
GO:0009894	regulation of catabolic process	4	1
GO:0009966	regulation of signal transduction	4	1
GO:0009967	positive regulation of signal transduction	5	1
GO:0009987	cellular process	1	1
GO:0009991	response to extracellular stimulus	3	1
GO:0010033	response to organic substance	3	1
GO:0010243	response to organonitrogen compound	4	1
GO:0010506	regulation of autophagy	6	1
GO:0010646	regulation of cell communication	4	1
GO:0010647	positive regulation of cell communication	5	1
GO:0019222	regulation of metabolic process	3	1
GO:0023051	regulation of signaling	3	1
GO:0023056	positive regulation of signaling	4	1
GO:0031323	regulation of cellular metabolic process	4	1
GO:0031329	regulation of cellular catabolic process	5	1
GO:0031667	response to nutrient levels	4	1
GO:0031668	cellular response to extracellular stimulus	3	1
GO:0031669	cellular response to nutrient levels	4	1
GO:0032006	regulation of TOR signaling	6	1
GO:0032008	positive regulation of TOR signaling	7	1
GO:0033554	cellular response to stress	3	1
GO:0042221	response to chemical	2	1
GO:0042594	response to starvation	3	1
GO:0043200	response to amino acid	4	1
GO:0048518	positive regulation of biological process	3	1
GO:0048522	positive regulation of cellular process	4	1
GO:0048583	regulation of response to stimulus	3	1
GO:0048584	positive regulation of response to stimulus	4	1
GO:0050789	regulation of biological process	2	1
GO:0050794	regulation of cellular process	3	1
GO:0050896	response to stimulus	1	1
GO:0051716	cellular response to stimulus	2	1
GO:0065007	biological regulation	1	1
GO:0070887	cellular response to chemical stimulus	3	1
GO:0071229	cellular response to acid chemical	4	1
GO:0071230	cellular response to amino acid stimulus	5	1
GO:0071310	cellular response to organic substance	4	1
GO:0071417	cellular response to organonitrogen compound	4	1
GO:0071495	cellular response to endogenous stimulus	3	1
GO:0071496	cellular response to external stimulus	3	1
GO:1901698	response to nitrogen compound	3	1
GO:1901699	cellular response to nitrogen compound	4	1
GO:1901700	response to oxygen-containing compound	3	1
GO:1901701	cellular response to oxygen-containing compound	4	1
GO:1902531	regulation of intracellular signal transduction	5	1
GO:1902533	positive regulation of intracellular signal transduction	6	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0017076	purine nucleotide binding	4	12
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003824	catalytic activity	1	1
GO:0003924	GTPase activity	7	1
GO:0016462	pyrophosphatase activity	5	1
GO:0016787	hydrolase activity	2	1
GO:0016817	hydrolase activity, acting on acid anhydrides	3	1
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	1
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	185	189	PF00656	0.208
CLV_C14_Caspase3-7	256	260	PF00656	0.148
CLV_NRD_NRD_1	175	177	PF00675	0.247
CLV_NRD_NRD_1	253	255	PF00675	0.312
CLV_NRD_NRD_1	341	343	PF00675	0.486
CLV_PCSK_KEX2_1	340	342	PF00082	0.490
CLV_PCSK_SKI1_1	16	20	PF00082	0.241
CLV_PCSK_SKI1_1	219	223	PF00082	0.278
CLV_PCSK_SKI1_1	262	266	PF00082	0.269
CLV_PCSK_SKI1_1	282	286	PF00082	0.333
CLV_PCSK_SKI1_1	41	45	PF00082	0.256
CLV_PCSK_SKI1_1	61	65	PF00082	0.112
CLV_PCSK_SKI1_1	75	79	PF00082	0.384
DOC_CYCLIN_RxL_1	58	69	PF00134	0.384
DOC_CYCLIN_yClb5_NLxxxL_5	61	67	PF00134	0.384
DOC_MAPK_gen_1	151	158	PF00069	0.317
DOC_MAPK_MEF2A_6	151	158	PF00069	0.317
DOC_MAPK_MEF2A_6	41	50	PF00069	0.241
DOC_MAPK_MEF2A_6	90	98	PF00069	0.241
DOC_MAPK_NFAT4_5	41	49	PF00069	0.241
DOC_PP1_RVXF_1	29	36	PF00149	0.241
DOC_USP7_MATH_1	207	211	PF00917	0.339
DOC_USP7_MATH_1	258	262	PF00917	0.279
DOC_USP7_UBL2_3	12	16	PF12436	0.534
DOC_WW_Pin1_4	146	151	PF00397	0.281
LIG_14-3-3_CanoR_1	243	247	PF00244	0.275
LIG_14-3-3_CanoR_1	273	281	PF00244	0.256
LIG_Actin_WH2_2	32	47	PF00022	0.241
LIG_APCC_ABBA_1	215	220	PF00400	0.275
LIG_APCC_ABBA_1	309	314	PF00400	0.373
LIG_BRCT_BRCA1_1	31	35	PF00533	0.241
LIG_Clathr_ClatBox_1	96	100	PF01394	0.241
LIG_eIF4E_1	91	97	PF01652	0.384
LIG_FHA_1	272	278	PF00498	0.256
LIG_FHA_1	290	296	PF00498	0.432
LIG_FHA_1	304	310	PF00498	0.290
LIG_FHA_1	314	320	PF00498	0.279
LIG_FHA_1	47	53	PF00498	0.290
LIG_FHA_1	76	82	PF00498	0.371
LIG_FHA_2	254	260	PF00498	0.161
LIG_LIR_Apic_2	126	132	PF02991	0.278
LIG_LIR_Gen_1	244	252	PF02991	0.257
LIG_LIR_Gen_1	302	312	PF02991	0.307
LIG_LIR_LC3C_4	316	321	PF02991	0.360
LIG_LIR_Nem_3	202	208	PF02991	0.281
LIG_LIR_Nem_3	244	249	PF02991	0.257
LIG_LIR_Nem_3	302	308	PF02991	0.341
LIG_LIR_Nem_3	32	38	PF02991	0.241
LIG_LIR_Nem_3	86	92	PF02991	0.244
LIG_LYPXL_SIV_4	207	215	PF13949	0.275
LIG_PDZ_Class_3	356	361	PF00595	0.440
LIG_Pex14_2	64	68	PF04695	0.384
LIG_RPA_C_Fungi	328	340	PF08784	0.465
LIG_SH2_CRK	208	212	PF00017	0.241
LIG_SH2_GRB2like	80	83	PF00017	0.317
LIG_SH2_PTP2	91	94	PF00017	0.384
LIG_SH2_STAP1	208	212	PF00017	0.259
LIG_SH2_STAT5	137	140	PF00017	0.241
LIG_SH2_STAT5	205	208	PF00017	0.281
LIG_SH2_STAT5	38	41	PF00017	0.241
LIG_SH2_STAT5	76	79	PF00017	0.303
LIG_SH2_STAT5	80	83	PF00017	0.377
LIG_SH2_STAT5	87	90	PF00017	0.335
LIG_SH2_STAT5	91	94	PF00017	0.404
LIG_SUMO_SIM_anti_2	316	322	PF11976	0.286
LIG_SUMO_SIM_par_1	316	322	PF11976	0.287
LIG_SUMO_SIM_par_1	48	53	PF11976	0.350
LIG_TYR_ITIM	206	211	PF00017	0.275
LIG_UBA3_1	157	166	PF00899	0.285
MOD_CDK_SPK_2	146	151	PF00069	0.317
MOD_CDK_SPxxK_3	146	153	PF00069	0.277
MOD_CK1_1	123	129	PF00069	0.407
MOD_CK1_1	198	204	PF00069	0.266
MOD_CK1_1	299	305	PF00069	0.460
MOD_GlcNHglycan	123	126	PF01048	0.401
MOD_GlcNHglycan	185	188	PF01048	0.262
MOD_GSK3_1	120	127	PF00069	0.443
MOD_GSK3_1	194	201	PF00069	0.282
MOD_GSK3_1	207	214	PF00069	0.296
MOD_GSK3_1	25	32	PF00069	0.241
MOD_GSK3_1	271	278	PF00069	0.308
MOD_GSK3_1	299	306	PF00069	0.369
MOD_GSK3_1	346	353	PF00069	0.442
MOD_GSK3_1	48	55	PF00069	0.293
MOD_N-GLC_1	289	294	PF02516	0.381
MOD_NEK2_1	194	199	PF00069	0.257
MOD_NEK2_1	206	211	PF00069	0.326
MOD_NEK2_1	222	227	PF00069	0.112
MOD_NEK2_1	247	252	PF00069	0.304
MOD_NEK2_1	284	289	PF00069	0.328
MOD_NEK2_1	46	51	PF00069	0.279
MOD_NEK2_1	52	57	PF00069	0.287
MOD_NEK2_2	200	205	PF00069	0.241
MOD_PIKK_1	52	58	PF00454	0.275
MOD_PKA_2	105	111	PF00069	0.275
MOD_PKA_2	242	248	PF00069	0.241
MOD_PKA_2	253	259	PF00069	0.241
MOD_PKA_2	272	278	PF00069	0.247
MOD_Plk_1	241	247	PF00069	0.283
MOD_Plk_1	290	296	PF00069	0.373
MOD_Plk_1	313	319	PF00069	0.301
MOD_Plk_1	52	58	PF00069	0.256
MOD_Plk_4	195	201	PF00069	0.285
MOD_Plk_4	207	213	PF00069	0.276
MOD_Plk_4	242	248	PF00069	0.241
MOD_ProDKin_1	146	152	PF00069	0.281
MOD_SUMO_rev_2	123	132	PF00179	0.346
MOD_SUMO_rev_2	322	327	PF00179	0.470
TRG_DiLeu_BaEn_2	139	145	PF01217	0.365
TRG_DiLeu_BaEn_2	241	247	PF01217	0.256
TRG_ENDOCYTIC_2	208	211	PF00928	0.275
TRG_ENDOCYTIC_2	91	94	PF00928	0.384
TRG_ER_diArg_1	340	342	PF00400	0.490
TRG_NES_CRM1_1	86	100	PF08389	0.241
TRG_Pf-PMV_PEXEL_1	143	147	PF00026	0.335

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEZ9	Leptomonas seymouri	85%	100%
A0A0S4INT4	Bodo saltans	45%	96%
A0A1X0NJF6	Trypanosomatidae	67%	100%
A0A3R7MBW5	Trypanosoma rangeli	64%	98%
A0A3S7WSQ4	Leishmania donovani	97%	99%
A4H7E6	Leishmania braziliensis	93%	100%
A4HVT5	Leishmania infantum	97%	99%
D0A6F6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
O74824	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	35%	100%
Q00582	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	33%	100%
Q3SX43	Bos taurus	36%	100%
Q4QFZ8	Leishmania major	96%	100%
Q54IK1	Dictyostelium discoideum	36%	100%
Q5VZM2	Homo sapiens	33%	97%
Q63486	Rattus norvegicus	36%	100%
Q63487	Rattus norvegicus	33%	97%
Q6NTA4	Mus musculus	33%	97%
Q7L523	Homo sapiens	36%	100%
Q80X95	Mus musculus	36%	100%
V5BM39	Trypanosoma cruzi	65%	99%