GPN-loop GTPase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

GPN-loop GTPase

Gene product:

XPA-interacting protein, putative

Species:

Leishmania mexicana

UniProt:

E9APF2_LEIMU

TriTrypDb:

LmxM.13.1280

Length:

327

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0005737	cytoplasm	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

E9APF2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APF2

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0003924	GTPase activity	7	12
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0016462	pyrophosphatase activity	5	12
GO:0016787	hydrolase activity	2	12
GO:0016817	hydrolase activity, acting on acid anhydrides	3	12
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	12
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	75	79	PF00656	0.538
CLV_NRD_NRD_1	224	226	PF00675	0.583
CLV_NRD_NRD_1	230	232	PF00675	0.601
CLV_PCSK_KEX2_1	224	226	PF00082	0.583
CLV_PCSK_KEX2_1	230	232	PF00082	0.601
CLV_PCSK_SKI1_1	118	122	PF00082	0.204
DEG_SPOP_SBC_1	15	19	PF00917	0.482
DEG_SPOP_SBC_1	2	6	PF00917	0.675
DOC_CKS1_1	127	132	PF01111	0.452
DOC_MAPK_DCC_7	186	194	PF00069	0.512
DOC_MAPK_HePTP_8	183	195	PF00069	0.452
DOC_MAPK_MEF2A_6	186	195	PF00069	0.452
DOC_USP7_MATH_1	223	227	PF00917	0.640
DOC_USP7_MATH_1	256	260	PF00917	0.709
DOC_USP7_MATH_1	293	297	PF00917	0.377
DOC_USP7_MATH_1	301	305	PF00917	0.435
DOC_WW_Pin1_4	10	15	PF00397	0.688
DOC_WW_Pin1_4	126	131	PF00397	0.452
DOC_WW_Pin1_4	166	171	PF00397	0.487
DOC_WW_Pin1_4	21	26	PF00397	0.458
DOC_WW_Pin1_4	65	70	PF00397	0.538
LIG_14-3-3_CanoR_1	165	170	PF00244	0.512
LIG_EH1_1	108	116	PF00400	0.463
LIG_FHA_1	153	159	PF00498	0.456
LIG_FHA_1	196	202	PF00498	0.468
LIG_FHA_1	21	27	PF00498	0.498
LIG_FHA_1	37	43	PF00498	0.384
LIG_HP1_1	25	29	PF01393	0.364
LIG_LIR_Gen_1	121	130	PF02991	0.452
LIG_LIR_Gen_1	285	295	PF02991	0.473
LIG_LIR_Gen_1	56	65	PF02991	0.476
LIG_LIR_Nem_3	121	127	PF02991	0.452
LIG_LIR_Nem_3	147	152	PF02991	0.490
LIG_LIR_Nem_3	285	290	PF02991	0.503
LIG_LIR_Nem_3	56	61	PF02991	0.476
LIG_LIR_Nem_3	79	85	PF02991	0.452
LIG_LRP6_Inhibitor_1	49	55	PF00058	0.323
LIG_Pex14_2	282	286	PF04695	0.441
LIG_SH2_CRK	292	296	PF00017	0.475
LIG_SH2_NCK_1	292	296	PF00017	0.425
LIG_SH2_NCK_1	48	52	PF00017	0.496
LIG_SH2_SRC	48	51	PF00017	0.496
LIG_SH2_STAT5	57	60	PF00017	0.463
LIG_SH3_3	124	130	PF00018	0.463
LIG_SH3_3	164	170	PF00018	0.538
LIG_SH3_3	217	223	PF00018	0.538
LIG_SUMO_SIM_anti_2	17	30	PF11976	0.567
LIG_SUMO_SIM_par_1	110	116	PF11976	0.492
LIG_TRAF2_1	265	268	PF00917	0.578
LIG_TRFH_1	89	93	PF08558	0.463
LIG_TYR_ITIM	290	295	PF00017	0.452
LIG_TYR_ITIM	80	85	PF00017	0.452
MOD_CK1_1	169	175	PF00069	0.476
MOD_CK1_1	20	26	PF00069	0.399
MOD_CK1_1	202	208	PF00069	0.538
MOD_CK1_1	226	232	PF00069	0.700
MOD_CK1_1	296	302	PF00069	0.467
MOD_CK2_1	110	116	PF00069	0.476
MOD_CK2_1	16	22	PF00069	0.549
MOD_GlcNHglycan	10	13	PF01048	0.683
MOD_GlcNHglycan	138	141	PF01048	0.242
MOD_GlcNHglycan	180	183	PF01048	0.338
MOD_GlcNHglycan	201	204	PF01048	0.319
MOD_GlcNHglycan	233	236	PF01048	0.700
MOD_GlcNHglycan	295	298	PF01048	0.373
MOD_GlcNHglycan	6	9	PF01048	0.694
MOD_GSK3_1	134	141	PF00069	0.447
MOD_GSK3_1	14	21	PF00069	0.633
MOD_GSK3_1	165	172	PF00069	0.464
MOD_GSK3_1	173	180	PF00069	0.465
MOD_GSK3_1	195	202	PF00069	0.463
MOD_GSK3_1	267	274	PF00069	0.523
MOD_GSK3_1	33	40	PF00069	0.421
MOD_GSK3_1	4	11	PF00069	0.678
MOD_N-GLC_1	195	200	PF02516	0.252
MOD_N-GLC_1	226	231	PF02516	0.686
MOD_NEK2_1	1	6	PF00069	0.701
MOD_NEK2_1	103	108	PF00069	0.452
MOD_NEK2_1	136	141	PF00069	0.448
MOD_NEK2_1	173	178	PF00069	0.449
MOD_NEK2_1	275	280	PF00069	0.403
MOD_NEK2_1	33	38	PF00069	0.463
MOD_PIKK_1	256	262	PF00454	0.657
MOD_PKA_1	118	124	PF00069	0.510
MOD_PKA_2	223	229	PF00069	0.645
MOD_PKA_2	76	82	PF00069	0.443
MOD_Plk_1	267	273	PF00069	0.366
MOD_Plk_4	110	116	PF00069	0.538
MOD_Plk_4	152	158	PF00069	0.444
MOD_Plk_4	169	175	PF00069	0.452
MOD_Plk_4	202	208	PF00069	0.485
MOD_Plk_4	277	283	PF00069	0.467
MOD_Plk_4	37	43	PF00069	0.463
MOD_ProDKin_1	10	16	PF00069	0.689
MOD_ProDKin_1	126	132	PF00069	0.452
MOD_ProDKin_1	166	172	PF00069	0.487
MOD_ProDKin_1	21	27	PF00069	0.445
MOD_ProDKin_1	65	71	PF00069	0.538
MOD_SUMO_rev_2	113	120	PF00179	0.496
TRG_ENDOCYTIC_2	287	290	PF00928	0.392
TRG_ENDOCYTIC_2	292	295	PF00928	0.342
TRG_ENDOCYTIC_2	82	85	PF00928	0.452
TRG_ER_diArg_1	223	225	PF00400	0.484

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0Y6	Leptomonas seymouri	77%	99%
A0A0S4IST9	Bodo saltans	49%	95%
A0A1X0NNH8	Trypanosomatidae	54%	100%
A0A3R7KPX1	Trypanosoma rangeli	59%	100%
A0A3S7WSM7	Leishmania donovani	95%	100%
A0A3S7WZ40	Leishmania donovani	31%	100%
A4FUD1	Bos taurus	42%	88%
A4H7B1	Leishmania braziliensis	88%	100%
A4HVQ6	Leishmania infantum	95%	100%
A4I1I3	Leishmania infantum	31%	100%
D0A6I5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	58%	100%
E9AXL8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
O42906	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	41%	89%
P46577	Caenorhabditis elegans	39%	92%
P47122	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	45%	85%
Q28I42	Xenopus tropicalis	30%	100%
Q4IQT8	Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1)	24%	100%
Q4Q9V4	Leishmania major	30%	100%
Q4QG26	Leishmania major	93%	100%
Q54C25	Dictyostelium discoideum	44%	83%
Q54TE7	Dictyostelium discoideum	25%	100%
Q56XY2	Arabidopsis thaliana	28%	100%
Q8VCE2	Mus musculus	42%	88%
Q8W586	Arabidopsis thaliana	42%	86%
Q9HCN4	Homo sapiens	43%	87%
V5A2Y8	Trypanosoma cruzi	60%	98%