DNA/RNA non-specific endonuclease-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA/RNA non-specific endonuclease-like protein

Species:

Leishmania mexicana

UniProt:

E9APF1_LEIMU

TriTrypDb:

LmxM.13.1270

Length:

808

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 5
NetGPI	no	yes: 0, no: 5

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0005743	mitochondrial inner membrane	5	1
GO:0016020	membrane	2	2
GO:0019866	organelle inner membrane	4	1
GO:0031090	organelle membrane	3	1
GO:0031966	mitochondrial membrane	4	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

E9APF1

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APF1

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	6
GO:0003824	catalytic activity	1	6
GO:0004518	nuclease activity	4	6
GO:0004519	endonuclease activity	5	6
GO:0005488	binding	1	6
GO:0016787	hydrolase activity	2	6
GO:0016788	hydrolase activity, acting on ester bonds	3	6
GO:0043167	ion binding	2	6
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6
GO:0097159	organic cyclic compound binding	2	6
GO:1901363	heterocyclic compound binding	2	6
GO:0000014	single-stranded DNA endodeoxyribonuclease activity	6	1
GO:0004520	DNA endonuclease activity	5	1
GO:0004521	RNA endonuclease activity	5	1
GO:0004536	DNA nuclease activity	4	1
GO:0004540	RNA nuclease activity	4	1
GO:0140097	catalytic activity, acting on DNA	3	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	765	769	PF00656	0.507
CLV_NRD_NRD_1	110	112	PF00675	0.739
CLV_NRD_NRD_1	123	125	PF00675	0.656
CLV_NRD_NRD_1	145	147	PF00675	0.692
CLV_NRD_NRD_1	160	162	PF00675	0.749
CLV_NRD_NRD_1	177	179	PF00675	0.617
CLV_NRD_NRD_1	330	332	PF00675	0.614
CLV_NRD_NRD_1	349	351	PF00675	0.411
CLV_NRD_NRD_1	429	431	PF00675	0.731
CLV_NRD_NRD_1	45	47	PF00675	0.475
CLV_NRD_NRD_1	470	472	PF00675	0.731
CLV_NRD_NRD_1	548	550	PF00675	0.602
CLV_NRD_NRD_1	555	557	PF00675	0.612
CLV_NRD_NRD_1	571	573	PF00675	0.492
CLV_NRD_NRD_1	625	627	PF00675	0.724
CLV_NRD_NRD_1	633	635	PF00675	0.764
CLV_NRD_NRD_1	693	695	PF00675	0.668
CLV_NRD_NRD_1	712	714	PF00675	0.615
CLV_PCSK_FUR_1	328	332	PF00082	0.551
CLV_PCSK_FUR_1	709	713	PF00082	0.686
CLV_PCSK_KEX2_1	110	112	PF00082	0.739
CLV_PCSK_KEX2_1	122	124	PF00082	0.667
CLV_PCSK_KEX2_1	145	147	PF00082	0.692
CLV_PCSK_KEX2_1	160	162	PF00082	0.749
CLV_PCSK_KEX2_1	177	179	PF00082	0.617
CLV_PCSK_KEX2_1	330	332	PF00082	0.612
CLV_PCSK_KEX2_1	349	351	PF00082	0.411
CLV_PCSK_KEX2_1	45	47	PF00082	0.475
CLV_PCSK_KEX2_1	470	472	PF00082	0.726
CLV_PCSK_KEX2_1	497	499	PF00082	0.796
CLV_PCSK_KEX2_1	548	550	PF00082	0.602
CLV_PCSK_KEX2_1	555	557	PF00082	0.612
CLV_PCSK_KEX2_1	632	634	PF00082	0.784
CLV_PCSK_KEX2_1	693	695	PF00082	0.668
CLV_PCSK_KEX2_1	709	711	PF00082	0.638
CLV_PCSK_KEX2_1	712	714	PF00082	0.615
CLV_PCSK_PC1ET2_1	122	124	PF00082	0.624
CLV_PCSK_PC1ET2_1	497	499	PF00082	0.796
CLV_PCSK_PC7_1	119	125	PF00082	0.619
CLV_PCSK_PC7_1	493	499	PF00082	0.680
CLV_PCSK_PC7_1	551	557	PF00082	0.582
CLV_PCSK_PC7_1	629	635	PF00082	0.650
CLV_PCSK_SKI1_1	119	123	PF00082	0.805
CLV_PCSK_SKI1_1	45	49	PF00082	0.476
CLV_PCSK_SKI1_1	684	688	PF00082	0.608
DEG_APCC_DBOX_1	123	131	PF00400	0.598
DEG_APCC_DBOX_1	185	193	PF00400	0.505
DEG_Nend_UBRbox_2	1	3	PF02207	0.679
DEG_SCF_FBW7_1	23	28	PF00400	0.597
DOC_ANK_TNKS_1	532	539	PF00023	0.535
DOC_CKS1_1	602	607	PF01111	0.469
DOC_CYCLIN_RxL_1	407	417	PF00134	0.316
DOC_CYCLIN_RxL_1	42	50	PF00134	0.672
DOC_CYCLIN_yCln2_LP_2	130	136	PF00134	0.535
DOC_CYCLIN_yCln2_LP_2	192	198	PF00134	0.504
DOC_MAPK_gen_1	119	129	PF00069	0.551
DOC_MAPK_gen_1	183	192	PF00069	0.505
DOC_MAPK_gen_1	568	576	PF00069	0.413
DOC_MAPK_gen_1	753	761	PF00069	0.352
DOC_MAPK_JIP1_4	186	192	PF00069	0.506
DOC_MAPK_MEF2A_6	20	29	PF00069	0.601
DOC_MAPK_MEF2A_6	570	578	PF00069	0.384
DOC_MAPK_MEF2A_6	753	761	PF00069	0.320
DOC_PP1_RVXF_1	208	215	PF00149	0.517
DOC_PP2B_LxvP_1	296	299	PF13499	0.412
DOC_PP2B_LxvP_1	35	38	PF13499	0.634
DOC_PP2B_LxvP_1	519	522	PF13499	0.469
DOC_PP2B_LxvP_1	651	654	PF13499	0.398
DOC_PP2B_LxvP_1	672	675	PF13499	0.405
DOC_PP4_FxxP_1	482	485	PF00568	0.533
DOC_USP7_MATH_1	152	156	PF00917	0.456
DOC_USP7_MATH_1	196	200	PF00917	0.505
DOC_USP7_MATH_1	236	240	PF00917	0.446
DOC_USP7_MATH_1	25	29	PF00917	0.723
DOC_USP7_MATH_1	367	371	PF00917	0.376
DOC_USP7_MATH_1	448	452	PF00917	0.581
DOC_USP7_MATH_1	496	500	PF00917	0.544
DOC_USP7_MATH_1	509	513	PF00917	0.351
DOC_USP7_MATH_1	55	59	PF00917	0.642
DOC_USP7_MATH_1	608	612	PF00917	0.511
DOC_USP7_MATH_1	692	696	PF00917	0.464
DOC_USP7_MATH_1	700	704	PF00917	0.457
DOC_USP7_MATH_1	723	727	PF00917	0.405
DOC_USP7_MATH_1	730	734	PF00917	0.365
DOC_USP7_MATH_1	75	79	PF00917	0.701
DOC_USP7_MATH_1	776	780	PF00917	0.512
DOC_WW_Pin1_4	102	107	PF00397	0.635
DOC_WW_Pin1_4	129	134	PF00397	0.538
DOC_WW_Pin1_4	191	196	PF00397	0.504
DOC_WW_Pin1_4	21	26	PF00397	0.612
DOC_WW_Pin1_4	290	295	PF00397	0.444
DOC_WW_Pin1_4	371	376	PF00397	0.316
DOC_WW_Pin1_4	454	459	PF00397	0.511
DOC_WW_Pin1_4	476	481	PF00397	0.533
DOC_WW_Pin1_4	601	606	PF00397	0.568
DOC_WW_Pin1_4	633	638	PF00397	0.572
DOC_WW_Pin1_4	642	647	PF00397	0.518
DOC_WW_Pin1_4	71	76	PF00397	0.711
LIG_14-3-3_CanoR_1	123	128	PF00244	0.492
LIG_14-3-3_CanoR_1	146	156	PF00244	0.496
LIG_14-3-3_CanoR_1	300	306	PF00244	0.294
LIG_14-3-3_CanoR_1	334	339	PF00244	0.351
LIG_14-3-3_CanoR_1	473	483	PF00244	0.567
LIG_14-3-3_CanoR_1	487	496	PF00244	0.469
LIG_14-3-3_CanoR_1	712	722	PF00244	0.487
LIG_14-3-3_CanoR_1	755	760	PF00244	0.354
LIG_14-3-3_CanoR_1	775	782	PF00244	0.436
LIG_14-3-3_CanoR_1	783	789	PF00244	0.430
LIG_BRCT_BRCA1_1	245	249	PF00533	0.477
LIG_BRCT_BRCA1_1	335	339	PF00533	0.217
LIG_BRCT_BRCA1_1	478	482	PF00533	0.529
LIG_BRCT_BRCA1_1	702	706	PF00533	0.463
LIG_deltaCOP1_diTrp_1	552	558	PF00928	0.481
LIG_DLG_GKlike_1	123	130	PF00625	0.418
LIG_EVH1_2	648	652	PF00568	0.332
LIG_FHA_1	157	163	PF00498	0.509
LIG_FHA_1	214	220	PF00498	0.562
LIG_FHA_1	22	28	PF00498	0.599
LIG_FHA_1	221	227	PF00498	0.496
LIG_FHA_1	238	244	PF00498	0.415
LIG_FHA_1	433	439	PF00498	0.541
LIG_FHA_1	542	548	PF00498	0.388
LIG_FHA_1	602	608	PF00498	0.468
LIG_FHA_1	67	73	PF00498	0.676
LIG_FHA_1	685	691	PF00498	0.437
LIG_FHA_1	736	742	PF00498	0.413
LIG_FHA_1	87	93	PF00498	0.412
LIG_FHA_2	115	121	PF00498	0.514
LIG_FHA_2	206	212	PF00498	0.552
LIG_FHA_2	763	769	PF00498	0.505
LIG_HCF-1_HBM_1	50	53	PF13415	0.681
LIG_Integrin_isoDGR_2	593	595	PF01839	0.650
LIG_LIR_Apic_2	479	485	PF02991	0.527
LIG_LIR_Apic_2	642	646	PF02991	0.491
LIG_LIR_Gen_1	784	792	PF02991	0.474
LIG_LIR_LC3C_4	733	736	PF02991	0.403
LIG_LIR_Nem_3	141	147	PF02991	0.522
LIG_LIR_Nem_3	304	310	PF02991	0.289
LIG_LIR_Nem_3	655	661	PF02991	0.371
LIG_LIR_Nem_3	784	789	PF02991	0.475
LIG_MLH1_MIPbox_1	335	339	PF16413	0.217
LIG_MYND_1	481	485	PF01753	0.430
LIG_PAM2_1	129	141	PF00658	0.494
LIG_PCNA_yPIPBox_3	392	402	PF02747	0.346
LIG_Pex14_1	316	320	PF04695	0.351
LIG_Pex14_2	658	662	PF04695	0.373
LIG_REV1ctd_RIR_1	336	346	PF16727	0.351
LIG_RPA_C_Fungi	689	701	PF08784	0.469
LIG_SH2_CRK	307	311	PF00017	0.412
LIG_SH2_NCK_1	307	311	PF00017	0.412
LIG_SH2_SRC	307	310	PF00017	0.412
LIG_SH2_STAT3	409	412	PF00017	0.349
LIG_SH2_STAT3	582	585	PF00017	0.480
LIG_SH2_STAT5	116	119	PF00017	0.644
LIG_SH2_STAT5	320	323	PF00017	0.434
LIG_SH2_STAT5	409	412	PF00017	0.412
LIG_SH2_STAT5	53	56	PF00017	0.627
LIG_SH3_1	634	640	PF00018	0.590
LIG_SH3_2	77	82	PF14604	0.661
LIG_SH3_3	2	8	PF00018	0.790
LIG_SH3_3	24	30	PF00018	0.578
LIG_SH3_3	455	461	PF00018	0.603
LIG_SH3_3	599	605	PF00018	0.579
LIG_SH3_3	634	640	PF00018	0.695
LIG_SH3_3	643	649	PF00018	0.642
LIG_SH3_3	672	678	PF00018	0.499
LIG_SH3_3	74	80	PF00018	0.697
LIG_SUMO_SIM_anti_2	31	36	PF11976	0.464
LIG_SUMO_SIM_par_1	737	742	PF11976	0.476
LIG_SUMO_SIM_par_1	747	754	PF11976	0.410
LIG_TRFH_1	786	790	PF08558	0.564
LIG_TYR_ITIM	305	310	PF00017	0.412
LIG_WW_3	459	463	PF00397	0.675
MOD_CDC14_SPxK_1	484	487	PF00782	0.664
MOD_CDK_SPxK_1	481	487	PF00069	0.701
MOD_CK1_1	185	191	PF00069	0.675
MOD_CK1_1	199	205	PF00069	0.537
MOD_CK1_1	239	245	PF00069	0.575
MOD_CK1_1	28	34	PF00069	0.567
MOD_CK1_1	333	339	PF00069	0.224
MOD_CK1_1	499	505	PF00069	0.649
MOD_CK1_1	559	565	PF00069	0.468
MOD_CK1_1	636	642	PF00069	0.712
MOD_CK1_1	70	76	PF00069	0.701
MOD_CK1_1	705	711	PF00069	0.594
MOD_CK1_1	726	732	PF00069	0.370
MOD_CK1_1	766	772	PF00069	0.671
MOD_CK2_1	114	120	PF00069	0.692
MOD_CK2_1	205	211	PF00069	0.702
MOD_CK2_1	734	740	PF00069	0.468
MOD_CK2_1	745	751	PF00069	0.397
MOD_CMANNOS	554	557	PF00535	0.639
MOD_Cter_Amidation	175	178	PF01082	0.483
MOD_Cter_Amidation	468	471	PF01082	0.605
MOD_Cter_Amidation	630	633	PF01082	0.547
MOD_GlcNHglycan	150	153	PF01048	0.553
MOD_GlcNHglycan	202	205	PF01048	0.637
MOD_GlcNHglycan	245	248	PF01048	0.469
MOD_GlcNHglycan	274	277	PF01048	0.659
MOD_GlcNHglycan	285	288	PF01048	0.566
MOD_GlcNHglycan	476	479	PF01048	0.685
MOD_GlcNHglycan	501	504	PF01048	0.636
MOD_GlcNHglycan	525	528	PF01048	0.656
MOD_GlcNHglycan	561	564	PF01048	0.658
MOD_GlcNHglycan	596	599	PF01048	0.609
MOD_GlcNHglycan	611	614	PF01048	0.507
MOD_GlcNHglycan	802	805	PF01048	0.607
MOD_GSK3_1	102	109	PF00069	0.663
MOD_GSK3_1	11	18	PF00069	0.634
MOD_GSK3_1	148	155	PF00069	0.615
MOD_GSK3_1	196	203	PF00069	0.667
MOD_GSK3_1	21	28	PF00069	0.565
MOD_GSK3_1	239	246	PF00069	0.559
MOD_GSK3_1	274	281	PF00069	0.668
MOD_GSK3_1	330	337	PF00069	0.383
MOD_GSK3_1	340	347	PF00069	0.389
MOD_GSK3_1	367	374	PF00069	0.491
MOD_GSK3_1	497	504	PF00069	0.625
MOD_GSK3_1	523	530	PF00069	0.612
MOD_GSK3_1	594	601	PF00069	0.698
MOD_GSK3_1	64	71	PF00069	0.700
MOD_GSK3_1	713	720	PF00069	0.670
MOD_GSK3_1	726	733	PF00069	0.418
MOD_GSK3_1	751	758	PF00069	0.513
MOD_GSK3_1	759	766	PF00069	0.564
MOD_GSK3_1	800	807	PF00069	0.609
MOD_LATS_1	198	204	PF00433	0.683
MOD_N-GLC_1	449	454	PF02516	0.569
MOD_NEK2_1	147	152	PF00069	0.611
MOD_NEK2_1	243	248	PF00069	0.590
MOD_NEK2_1	344	349	PF00069	0.412
MOD_NEK2_1	620	625	PF00069	0.567
MOD_NEK2_1	657	662	PF00069	0.440
MOD_NEK2_1	68	73	PF00069	0.565
MOD_NEK2_1	734	739	PF00069	0.476
MOD_NEK2_1	745	750	PF00069	0.355
MOD_NEK2_1	83	88	PF00069	0.364
MOD_NEK2_2	488	493	PF00069	0.533
MOD_NEK2_2	55	60	PF00069	0.494
MOD_NEK2_2	598	603	PF00069	0.535
MOD_PIKK_1	114	120	PF00454	0.692
MOD_PIKK_1	421	427	PF00454	0.504
MOD_PIKK_1	449	455	PF00454	0.756
MOD_PIKK_1	75	81	PF00454	0.620
MOD_PK_1	755	761	PF00069	0.379
MOD_PKA_1	123	129	PF00069	0.523
MOD_PKA_1	330	336	PF00069	0.377
MOD_PKA_1	430	436	PF00069	0.608
MOD_PKA_1	497	503	PF00069	0.700
MOD_PKA_1	693	699	PF00069	0.547
MOD_PKA_2	123	129	PF00069	0.523
MOD_PKA_2	182	188	PF00069	0.675
MOD_PKA_2	199	205	PF00069	0.505
MOD_PKA_2	301	307	PF00069	0.293
MOD_PKA_2	322	328	PF00069	0.435
MOD_PKA_2	330	336	PF00069	0.345
MOD_PKA_2	486	492	PF00069	0.628
MOD_PKA_2	497	503	PF00069	0.672
MOD_PKA_2	509	515	PF00069	0.451
MOD_PKA_2	583	589	PF00069	0.514
MOD_PKA_2	594	600	PF00069	0.657
MOD_PKA_2	692	698	PF00069	0.583
MOD_PKA_2	714	720	PF00069	0.607
MOD_PKA_2	766	772	PF00069	0.671
MOD_PKB_1	328	336	PF00069	0.274
MOD_PKB_1	428	436	PF00069	0.604
MOD_PKB_1	711	719	PF00069	0.607
MOD_PKB_1	753	761	PF00069	0.556
MOD_Plk_1	25	31	PF00069	0.511
MOD_Plk_1	340	346	PF00069	0.412
MOD_Plk_4	138	144	PF00069	0.614
MOD_Plk_4	185	191	PF00069	0.675
MOD_Plk_4	28	34	PF00069	0.600
MOD_Plk_4	334	340	PF00069	0.274
MOD_Plk_4	647	653	PF00069	0.537
MOD_Plk_4	745	751	PF00069	0.486
MOD_ProDKin_1	102	108	PF00069	0.636
MOD_ProDKin_1	129	135	PF00069	0.672
MOD_ProDKin_1	191	197	PF00069	0.625
MOD_ProDKin_1	21	27	PF00069	0.493
MOD_ProDKin_1	290	296	PF00069	0.529
MOD_ProDKin_1	371	377	PF00069	0.362
MOD_ProDKin_1	454	460	PF00069	0.629
MOD_ProDKin_1	476	482	PF00069	0.669
MOD_ProDKin_1	601	607	PF00069	0.714
MOD_ProDKin_1	633	639	PF00069	0.719
MOD_ProDKin_1	642	648	PF00069	0.636
MOD_ProDKin_1	71	77	PF00069	0.630
TRG_DiLeu_BaEn_1	668	673	PF01217	0.487
TRG_DiLeu_BaLyEn_6	125	130	PF01217	0.552
TRG_DiLeu_BaLyEn_6	291	296	PF01217	0.565
TRG_DiLeu_BaLyEn_6	43	48	PF01217	0.629
TRG_DiLeu_BaLyEn_6	514	519	PF01217	0.519
TRG_ENDOCYTIC_2	307	310	PF00928	0.474
TRG_ER_diArg_1	110	112	PF00400	0.487
TRG_ER_diArg_1	144	146	PF00400	0.601
TRG_ER_diArg_1	160	162	PF00400	0.689
TRG_ER_diArg_1	177	179	PF00400	0.504
TRG_ER_diArg_1	299	302	PF00400	0.295
TRG_ER_diArg_1	328	331	PF00400	0.453
TRG_ER_diArg_1	349	351	PF00400	0.409
TRG_ER_diArg_1	45	47	PF00400	0.585
TRG_ER_diArg_1	470	473	PF00400	0.684
TRG_ER_diArg_1	547	549	PF00400	0.487
TRG_ER_diArg_1	554	556	PF00400	0.496
TRG_ER_diArg_1	632	634	PF00400	0.649
TRG_ER_diArg_1	709	712	PF00400	0.616
TRG_ER_diArg_1	752	755	PF00400	0.495
TRG_ER_diArg_1	792	795	PF00400	0.627
TRG_NES_CRM1_1	725	740	PF08389	0.458
TRG_NLS_Bipartite_1	110	126	PF00514	0.500
TRG_NLS_MonoExtN_4	119	126	PF00514	0.503
TRG_NLS_MonoExtN_4	624	630	PF00514	0.521
TRG_Pf-PMV_PEXEL_1	178	182	PF00026	0.626
TRG_Pf-PMV_PEXEL_1	45	50	PF00026	0.636

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A3Q8I9Q8	Leishmania donovani	85%	99%
A4H7B0	Leishmania braziliensis	65%	100%
A4HVQ5	Leishmania infantum	85%	92%
Q4QG27	Leishmania major	83%	100%