TPK_B1_binding domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Thiamin pyrophosphokinase, vitamin B1 binding domain containing protein, putative

Species:

Leishmania mexicana

UniProt:

E9APE8_LEIMU

TriTrypDb:

LmxM.13.1240

Length:

544

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

E9APE8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APE8

Function

Biological processes
Term	Name	Level	Count
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006766	vitamin metabolic process	3	11
GO:0006767	water-soluble vitamin metabolic process	4	11
GO:0006790	sulfur compound metabolic process	3	11
GO:0006793	phosphorus metabolic process	3	11
GO:0006796	phosphate-containing compound metabolic process	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009110	vitamin biosynthetic process	4	11
GO:0009229	thiamine diphosphate biosynthetic process	5	11
GO:0009987	cellular process	1	11
GO:0016310	phosphorylation	5	10
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0019637	organophosphate metabolic process	3	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0042357	thiamine diphosphate metabolic process	4	11
GO:0042364	water-soluble vitamin biosynthetic process	5	11
GO:0042723	thiamine-containing compound metabolic process	4	11
GO:0042724	thiamine-containing compound biosynthetic process	5	11
GO:0044237	cellular metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0044272	sulfur compound biosynthetic process	4	11
GO:0044281	small molecule metabolic process	2	11
GO:0044283	small molecule biosynthetic process	3	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0072527	pyrimidine-containing compound metabolic process	4	11
GO:0072528	pyrimidine-containing compound biosynthetic process	5	11
GO:0090407	organophosphate biosynthetic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901564	organonitrogen compound metabolic process	3	11
GO:1901566	organonitrogen compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004788	thiamine diphosphokinase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016778	diphosphotransferase activity	4	11
GO:0017076	purine nucleotide binding	4	11
GO:0019842	vitamin binding	3	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0030975	thiamine binding	3	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	11
GO:0043178	alcohol binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11
GO:1901681	sulfur compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	168	172	PF00656	0.568
CLV_C14_Caspase3-7	205	209	PF00656	0.300
CLV_NRD_NRD_1	18	20	PF00675	0.454
CLV_NRD_NRD_1	187	189	PF00675	0.731
CLV_NRD_NRD_1	221	223	PF00675	0.555
CLV_NRD_NRD_1	330	332	PF00675	0.597
CLV_PCSK_FUR_1	219	223	PF00082	0.559
CLV_PCSK_FUR_1	328	332	PF00082	0.421
CLV_PCSK_KEX2_1	117	119	PF00082	0.487
CLV_PCSK_KEX2_1	187	189	PF00082	0.720
CLV_PCSK_KEX2_1	199	201	PF00082	0.618
CLV_PCSK_KEX2_1	219	221	PF00082	0.471
CLV_PCSK_KEX2_1	330	332	PF00082	0.546
CLV_PCSK_PC1ET2_1	117	119	PF00082	0.487
CLV_PCSK_PC1ET2_1	199	201	PF00082	0.618
CLV_PCSK_SKI1_1	199	203	PF00082	0.593
CLV_PCSK_SKI1_1	330	334	PF00082	0.509
CLV_PCSK_SKI1_1	468	472	PF00082	0.362
CLV_PCSK_SKI1_1	494	498	PF00082	0.390
DEG_APCC_DBOX_1	329	337	PF00400	0.503
DEG_SPOP_SBC_1	232	236	PF00917	0.446
DOC_CYCLIN_RxL_1	327	337	PF00134	0.504
DOC_MAPK_MEF2A_6	354	361	PF00069	0.530
DOC_PP1_RVXF_1	197	204	PF00149	0.606
DOC_PP1_RVXF_1	466	472	PF00149	0.586
DOC_PP2B_LxvP_1	527	530	PF13499	0.572
DOC_PP2B_PxIxI_1	356	362	PF00149	0.442
DOC_PP4_FxxP_1	408	411	PF00568	0.323
DOC_USP7_MATH_1	137	141	PF00917	0.524
DOC_USP7_MATH_1	230	234	PF00917	0.536
DOC_USP7_MATH_1	416	420	PF00917	0.404
DOC_USP7_MATH_1	79	83	PF00917	0.784
DOC_WW_Pin1_4	233	238	PF00397	0.612
DOC_WW_Pin1_4	291	296	PF00397	0.644
DOC_WW_Pin1_4	334	339	PF00397	0.561
DOC_WW_Pin1_4	412	417	PF00397	0.437
DOC_WW_Pin1_4	480	485	PF00397	0.593
DOC_WW_Pin1_4	52	57	PF00397	0.417
LIG_14-3-3_CanoR_1	19	28	PF00244	0.530
LIG_Actin_WH2_2	481	496	PF00022	0.377
LIG_APCC_ABBAyCdc20_2	199	205	PF00400	0.658
LIG_BIR_II_1	1	5	PF00653	0.726
LIG_BIR_III_2	181	185	PF00653	0.696
LIG_BRCT_BRCA1_1	250	254	PF00533	0.462
LIG_BRCT_BRCA1_2	250	256	PF00533	0.452
LIG_EVH1_2	521	525	PF00568	0.417
LIG_FHA_1	10	16	PF00498	0.702
LIG_FHA_1	233	239	PF00498	0.651
LIG_FHA_1	253	259	PF00498	0.497
LIG_FHA_1	31	37	PF00498	0.699
LIG_FHA_1	351	357	PF00498	0.461
LIG_FHA_1	404	410	PF00498	0.366
LIG_FHA_1	441	447	PF00498	0.544
LIG_FHA_1	480	486	PF00498	0.518
LIG_FHA_1	524	530	PF00498	0.554
LIG_FHA_2	234	240	PF00498	0.696
LIG_FHA_2	57	63	PF00498	0.491
LIG_LIR_Apic_2	406	411	PF02991	0.311
LIG_LIR_Gen_1	110	121	PF02991	0.475
LIG_LIR_Gen_1	122	128	PF02991	0.340
LIG_LIR_Gen_1	239	247	PF02991	0.567
LIG_LIR_Nem_3	110	116	PF02991	0.390
LIG_LIR_Nem_3	122	127	PF02991	0.330
LIG_LIR_Nem_3	239	244	PF02991	0.572
LIG_LIR_Nem_3	370	376	PF02991	0.491
LIG_LIR_Nem_3	45	51	PF02991	0.531
LIG_LIR_Nem_3	456	461	PF02991	0.572
LIG_LIR_Nem_3	60	66	PF02991	0.280
LIG_PTAP_UEV_1	337	342	PF05743	0.493
LIG_SH2_CRK	113	117	PF00017	0.507
LIG_SH2_CRK	382	386	PF00017	0.508
LIG_SH2_CRK	394	398	PF00017	0.442
LIG_SH2_NCK_1	382	386	PF00017	0.511
LIG_SH2_PTP2	124	127	PF00017	0.337
LIG_SH2_STAP1	382	386	PF00017	0.511
LIG_SH2_STAT5	105	108	PF00017	0.360
LIG_SH2_STAT5	119	122	PF00017	0.425
LIG_SH2_STAT5	124	127	PF00017	0.484
LIG_SH2_STAT5	428	431	PF00017	0.188
LIG_SH2_STAT5	461	464	PF00017	0.586
LIG_SH2_STAT5	66	69	PF00017	0.412
LIG_SH3_3	234	240	PF00018	0.705
LIG_SH3_3	24	30	PF00018	0.313
LIG_SH3_3	335	341	PF00018	0.619
LIG_SH3_3	478	484	PF00018	0.591
LIG_SH3_3	496	502	PF00018	0.482
LIG_SH3_3	511	517	PF00018	0.650
LIG_SUMO_SIM_par_1	482	489	PF11976	0.502
LIG_SUMO_SIM_par_1	49	55	PF11976	0.417
LIG_TRFH_1	241	245	PF08558	0.486
LIG_TRFH_1	408	412	PF08558	0.348
LIG_TYR_ITAM	110	127	PF00017	0.280
LIG_TYR_ITIM	380	385	PF00017	0.520
LIG_UBA3_1	388	393	PF00899	0.519
MOD_CK1_1	132	138	PF00069	0.568
MOD_CK1_1	140	146	PF00069	0.600
MOD_CK1_1	231	237	PF00069	0.539
MOD_CK1_1	299	305	PF00069	0.395
MOD_CK2_1	233	239	PF00069	0.711
MOD_CK2_1	310	316	PF00069	0.504
MOD_CK2_1	79	85	PF00069	0.494
MOD_GlcNHglycan	129	132	PF01048	0.701
MOD_GlcNHglycan	139	142	PF01048	0.604
MOD_GlcNHglycan	158	161	PF01048	0.344
MOD_GlcNHglycan	229	233	PF01048	0.673
MOD_GlcNHglycan	338	341	PF01048	0.694
MOD_GlcNHglycan	416	419	PF01048	0.621
MOD_GlcNHglycan	475	478	PF01048	0.321
MOD_GlcNHglycan	503	506	PF01048	0.691
MOD_GlcNHglycan	81	84	PF01048	0.502
MOD_GSK3_1	123	130	PF00069	0.608
MOD_GSK3_1	133	140	PF00069	0.555
MOD_GSK3_1	154	161	PF00069	0.553
MOD_GSK3_1	228	235	PF00069	0.705
MOD_GSK3_1	248	255	PF00069	0.591
MOD_GSK3_1	291	298	PF00069	0.606
MOD_GSK3_1	30	37	PF00069	0.749
MOD_GSK3_1	332	339	PF00069	0.559
MOD_GSK3_1	410	417	PF00069	0.602
MOD_GSK3_1	473	480	PF00069	0.532
MOD_GSK3_1	52	59	PF00069	0.476
MOD_NEK2_1	142	147	PF00069	0.574
MOD_NEK2_1	158	163	PF00069	0.351
MOD_NEK2_1	228	233	PF00069	0.679
MOD_NEK2_1	332	337	PF00069	0.589
MOD_NEK2_1	350	355	PF00069	0.374
MOD_NEK2_1	421	426	PF00069	0.469
MOD_NEK2_2	277	282	PF00069	0.457
MOD_PIKK_1	403	409	PF00454	0.365
MOD_PIKK_1	459	465	PF00454	0.586
MOD_PIKK_1	9	15	PF00454	0.726
MOD_PKA_2	421	427	PF00069	0.645
MOD_PKB_1	220	228	PF00069	0.589
MOD_Plk_1	295	301	PF00069	0.616
MOD_Plk_4	520	526	PF00069	0.454
MOD_ProDKin_1	233	239	PF00069	0.605
MOD_ProDKin_1	291	297	PF00069	0.632
MOD_ProDKin_1	334	340	PF00069	0.575
MOD_ProDKin_1	412	418	PF00069	0.447
MOD_ProDKin_1	480	486	PF00069	0.593
MOD_ProDKin_1	52	58	PF00069	0.412
TRG_DiLeu_BaEn_1	239	244	PF01217	0.515
TRG_DiLeu_BaEn_1	442	447	PF01217	0.475
TRG_DiLeu_BaLyEn_6	197	202	PF01217	0.533
TRG_DiLeu_BaLyEn_6	328	333	PF01217	0.397
TRG_ENDOCYTIC_2	113	116	PF00928	0.410
TRG_ENDOCYTIC_2	124	127	PF00928	0.414
TRG_ENDOCYTIC_2	382	385	PF00928	0.510
TRG_ENDOCYTIC_2	394	397	PF00928	0.451
TRG_ENDOCYTIC_2	66	69	PF00928	0.412
TRG_ER_diArg_1	218	221	PF00400	0.514
TRG_ER_diArg_1	328	331	PF00400	0.481
TRG_ER_diArg_1	74	77	PF00400	0.590
TRG_NES_CRM1_1	279	293	PF08389	0.543
TRG_Pf-PMV_PEXEL_1	200	205	PF00026	0.474
TRG_Pf-PMV_PEXEL_1	330	334	PF00026	0.485
TRG_Pf-PMV_PEXEL_1	97	101	PF00026	0.439

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFZ8	Leptomonas seymouri	44%	100%
A0A1X0NMY7	Trypanosomatidae	30%	100%
A0A3Q8IBS6	Leishmania donovani	86%	99%
A0A422N0R9	Trypanosoma rangeli	27%	100%
A4H7A7	Leishmania braziliensis	70%	100%
A4HVQ2	Leishmania infantum	87%	100%
D0A6I9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	100%
Q4QG30	Leishmania major	85%	100%
V5ARR9	Trypanosoma cruzi	27%	100%