Adenylosuccinate synthetase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

adenylosuccinate synthetase, putative

Species:

Leishmania mexicana

UniProt:

E9APE3_LEIMU

TriTrypDb:

LmxM.13.1190

Length:

712

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 7

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	12
GO:0110165	cellular anatomical entity	1	12
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1

Expansion

Sequence features

E9APE3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APE3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006163	purine nucleotide metabolic process	5	12
GO:0006164	purine nucleotide biosynthetic process	6	12
GO:0006167	AMP biosynthetic process	8	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006753	nucleoside phosphate metabolic process	4	12
GO:0006793	phosphorus metabolic process	3	12
GO:0006796	phosphate-containing compound metabolic process	4	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009117	nucleotide metabolic process	5	12
GO:0009123	nucleoside monophosphate metabolic process	5	12
GO:0009124	nucleoside monophosphate biosynthetic process	6	12
GO:0009126	purine nucleoside monophosphate metabolic process	6	12
GO:0009127	purine nucleoside monophosphate biosynthetic process	7	12
GO:0009150	purine ribonucleotide metabolic process	6	12
GO:0009152	purine ribonucleotide biosynthetic process	7	12
GO:0009156	ribonucleoside monophosphate biosynthetic process	7	12
GO:0009161	ribonucleoside monophosphate metabolic process	6	12
GO:0009165	nucleotide biosynthetic process	6	12
GO:0009167	purine ribonucleoside monophosphate metabolic process	7	12
GO:0009168	purine ribonucleoside monophosphate biosynthetic process	8	12
GO:0009259	ribonucleotide metabolic process	5	12
GO:0009260	ribonucleotide biosynthetic process	6	12
GO:0009987	cellular process	1	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0019637	organophosphate metabolic process	3	12
GO:0019693	ribose phosphate metabolic process	4	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0044208	'de novo' AMP biosynthetic process	9	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044281	small molecule metabolic process	2	12
GO:0046033	AMP metabolic process	7	12
GO:0046390	ribose phosphate biosynthetic process	5	12
GO:0046483	heterocycle metabolic process	3	12
GO:0055086	nucleobase-containing small molecule metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0072521	purine-containing compound metabolic process	4	12
GO:0072522	purine-containing compound biosynthetic process	5	12
GO:0090407	organophosphate biosynthetic process	4	12
GO:1901135	carbohydrate derivative metabolic process	3	12
GO:1901137	carbohydrate derivative biosynthetic process	4	12
GO:1901293	nucleoside phosphate biosynthetic process	5	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12
GO:0046040	IMP metabolic process	7	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0000287	magnesium ion binding	5	12
GO:0003824	catalytic activity	1	12
GO:0004019	adenylosuccinate synthase activity	4	12
GO:0005488	binding	1	12
GO:0005525	GTP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0017076	purine nucleotide binding	4	12
GO:0019001	guanyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032561	guanyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	159	163	PF00656	0.523
CLV_C14_Caspase3-7	358	362	PF00656	0.504
CLV_C14_Caspase3-7	58	62	PF00656	0.736
CLV_NRD_NRD_1	150	152	PF00675	0.655
CLV_NRD_NRD_1	220	222	PF00675	0.315
CLV_NRD_NRD_1	326	328	PF00675	0.391
CLV_NRD_NRD_1	4	6	PF00675	0.790
CLV_NRD_NRD_1	671	673	PF00675	0.320
CLV_PCSK_KEX2_1	150	152	PF00082	0.655
CLV_PCSK_KEX2_1	237	239	PF00082	0.314
CLV_PCSK_KEX2_1	311	313	PF00082	0.312
CLV_PCSK_KEX2_1	328	330	PF00082	0.268
CLV_PCSK_KEX2_1	4	6	PF00082	0.790
CLV_PCSK_KEX2_1	669	671	PF00082	0.304
CLV_PCSK_PC1ET2_1	237	239	PF00082	0.315
CLV_PCSK_PC1ET2_1	311	313	PF00082	0.312
CLV_PCSK_PC1ET2_1	328	330	PF00082	0.268
CLV_PCSK_PC1ET2_1	669	671	PF00082	0.304
CLV_PCSK_SKI1_1	150	154	PF00082	0.562
CLV_PCSK_SKI1_1	222	226	PF00082	0.294
CLV_PCSK_SKI1_1	308	312	PF00082	0.410
CLV_PCSK_SKI1_1	338	342	PF00082	0.238
CLV_PCSK_SKI1_1	416	420	PF00082	0.334
CLV_PCSK_SKI1_1	514	518	PF00082	0.362
CLV_Separin_Metazoa	324	328	PF03568	0.482
DEG_SPOP_SBC_1	105	109	PF00917	0.552
DEG_SPOP_SBC_1	293	297	PF00917	0.504
DOC_CDC14_PxL_1	140	148	PF14671	0.492
DOC_CKS1_1	278	283	PF01111	0.504
DOC_CYCLIN_RxL_1	281	291	PF00134	0.515
DOC_CYCLIN_RxL_1	511	519	PF00134	0.598
DOC_CYCLIN_yCln2_LP_2	92	98	PF00134	0.524
DOC_MAPK_gen_1	308	316	PF00069	0.503
DOC_MAPK_gen_1	377	386	PF00069	0.578
DOC_MAPK_gen_1	458	466	PF00069	0.552
DOC_MAPK_MEF2A_6	458	466	PF00069	0.498
DOC_MAPK_MEF2A_6	545	552	PF00069	0.482
DOC_MAPK_MEF2A_6	71	79	PF00069	0.789
DOC_MAPK_RevD_3	225	238	PF00069	0.564
DOC_PP1_RVXF_1	377	383	PF00149	0.564
DOC_PP2B_LxvP_1	232	235	PF13499	0.609
DOC_PP4_FxxP_1	70	73	PF00568	0.718
DOC_USP7_MATH_1	105	109	PF00917	0.581
DOC_USP7_MATH_1	136	140	PF00917	0.631
DOC_USP7_MATH_1	15	19	PF00917	0.792
DOC_USP7_MATH_1	292	296	PF00917	0.507
DOC_USP7_MATH_1	36	40	PF00917	0.778
DOC_USP7_MATH_1	470	474	PF00917	0.504
DOC_USP7_MATH_1	479	483	PF00917	0.504
DOC_USP7_MATH_1	553	557	PF00917	0.528
DOC_USP7_MATH_1	640	644	PF00917	0.530
DOC_USP7_UBL2_3	218	222	PF12436	0.590
DOC_USP7_UBL2_3	328	332	PF12436	0.609
DOC_WW_Pin1_4	11	16	PF00397	0.813
DOC_WW_Pin1_4	19	24	PF00397	0.762
DOC_WW_Pin1_4	277	282	PF00397	0.504
DOC_WW_Pin1_4	29	34	PF00397	0.744
DOC_WW_Pin1_4	660	665	PF00397	0.435
LIG_14-3-3_CanoR_1	129	134	PF00244	0.524
LIG_14-3-3_CanoR_1	9	15	PF00244	0.784
LIG_AP2alpha_1	517	521	PF02296	0.609
LIG_APCC_ABBA_1	384	389	PF00400	0.515
LIG_EH1_1	650	658	PF00400	0.515
LIG_FHA_1	402	408	PF00498	0.604
LIG_FHA_2	212	218	PF00498	0.504
LIG_FHA_2	42	48	PF00498	0.783
LIG_FHA_2	437	443	PF00498	0.497
LIG_FHA_2	495	501	PF00498	0.515
LIG_Integrin_RGD_1	599	601	PF01839	0.304
LIG_IRF3_LxIS_1	590	595	PF10401	0.504
LIG_LIR_Gen_1	132	142	PF02991	0.574
LIG_LIR_Gen_1	280	289	PF02991	0.504
LIG_LIR_Gen_1	601	611	PF02991	0.504
LIG_LIR_Nem_3	120	125	PF02991	0.601
LIG_LIR_Nem_3	132	137	PF02991	0.460
LIG_LIR_Nem_3	162	168	PF02991	0.436
LIG_LIR_Nem_3	280	285	PF02991	0.504
LIG_LIR_Nem_3	335	340	PF02991	0.505
LIG_LIR_Nem_3	601	606	PF02991	0.504
LIG_MAD2	694	702	PF02301	0.564
LIG_NRBOX	284	290	PF00104	0.515
LIG_Pex14_2	517	521	PF04695	0.528
LIG_PTB_Apo_2	391	398	PF02174	0.590
LIG_SH2_CRK	282	286	PF00017	0.504
LIG_SH2_CRK	337	341	PF00017	0.485
LIG_SH2_CRK	603	607	PF00017	0.528
LIG_SH2_GRB2like	125	128	PF00017	0.598
LIG_SH2_GRB2like	55	58	PF00017	0.651
LIG_SH2_NCK_1	155	159	PF00017	0.369
LIG_SH2_NCK_1	216	220	PF00017	0.564
LIG_SH2_NCK_1	603	607	PF00017	0.528
LIG_SH2_PTP2	240	243	PF00017	0.528
LIG_SH2_SRC	369	372	PF00017	0.609
LIG_SH2_STAP1	101	105	PF00017	0.616
LIG_SH2_STAP1	405	409	PF00017	0.615
LIG_SH2_STAT3	709	712	PF00017	0.454
LIG_SH2_STAT5	122	125	PF00017	0.462
LIG_SH2_STAT5	155	158	PF00017	0.407
LIG_SH2_STAT5	240	243	PF00017	0.515
LIG_SH2_STAT5	369	372	PF00017	0.522
LIG_SH2_STAT5	489	492	PF00017	0.516
LIG_SH2_STAT5	591	594	PF00017	0.504
LIG_SH2_STAT5	612	615	PF00017	0.504
LIG_SH2_STAT5	689	692	PF00017	0.590
LIG_SH2_STAT5	709	712	PF00017	0.271
LIG_SH3_1	392	398	PF00018	0.590
LIG_SH3_2	395	400	PF14604	0.590
LIG_SH3_3	243	249	PF00018	0.504
LIG_SH3_3	27	33	PF00018	0.789
LIG_SH3_3	361	367	PF00018	0.581
LIG_SH3_3	392	398	PF00018	0.590
LIG_TRFH_1	225	229	PF08558	0.504
MOD_CDK_SPxxK_3	277	284	PF00069	0.504
MOD_CK1_1	108	114	PF00069	0.713
MOD_CK1_1	120	126	PF00069	0.487
MOD_CK1_1	22	28	PF00069	0.762
MOD_CK1_1	437	443	PF00069	0.504
MOD_CK1_1	540	546	PF00069	0.571
MOD_CK2_1	129	135	PF00069	0.572
MOD_CK2_1	248	254	PF00069	0.497
MOD_CK2_1	682	688	PF00069	0.564
MOD_Cter_Amidation	219	222	PF01082	0.315
MOD_Cter_Amidation	235	238	PF01082	0.344
MOD_GlcNHglycan	108	111	PF01048	0.710
MOD_GlcNHglycan	119	122	PF01048	0.533
MOD_GlcNHglycan	206	209	PF01048	0.294
MOD_GlcNHglycan	229	232	PF01048	0.315
MOD_GlcNHglycan	290	293	PF01048	0.301
MOD_GlcNHglycan	439	442	PF01048	0.294
MOD_GlcNHglycan	490	493	PF01048	0.308
MOD_GlcNHglycan	502	505	PF01048	0.304
MOD_GlcNHglycan	555	558	PF01048	0.342
MOD_GlcNHglycan	594	597	PF01048	0.304
MOD_GlcNHglycan	644	647	PF01048	0.353
MOD_GSK3_1	104	111	PF00069	0.746
MOD_GSK3_1	11	18	PF00069	0.825
MOD_GSK3_1	113	120	PF00069	0.604
MOD_GSK3_1	156	163	PF00069	0.531
MOD_GSK3_1	19	26	PF00069	0.772
MOD_GSK3_1	288	295	PF00069	0.504
MOD_GSK3_1	36	43	PF00069	0.705
MOD_GSK3_1	421	428	PF00069	0.498
MOD_GSK3_1	436	443	PF00069	0.474
MOD_N-GLC_1	10	15	PF02516	0.783
MOD_N-GLC_1	105	110	PF02516	0.769
MOD_N-GLC_1	293	298	PF02516	0.304
MOD_N-GLC_1	344	349	PF02516	0.390
MOD_N-GLC_2	275	277	PF02516	0.390
MOD_NEK2_1	160	165	PF00069	0.516
MOD_NEK2_1	288	293	PF00069	0.511
MOD_NEK2_1	41	46	PF00069	0.686
MOD_NEK2_1	434	439	PF00069	0.504
MOD_NEK2_1	449	454	PF00069	0.504
MOD_NEK2_1	592	597	PF00069	0.509
MOD_NEK2_2	136	141	PF00069	0.632
MOD_NEK2_2	211	216	PF00069	0.500
MOD_PIKK_1	449	455	PF00454	0.588
MOD_PK_1	129	135	PF00069	0.311
MOD_PK_1	458	464	PF00069	0.552
MOD_PK_1	694	700	PF00069	0.504
MOD_PK_1	71	77	PF00069	0.788
MOD_PK_1	94	100	PF00069	0.829
MOD_Plk_1	156	162	PF00069	0.490
MOD_Plk_1	293	299	PF00069	0.504
MOD_Plk_1	401	407	PF00069	0.553
MOD_Plk_2-3	248	254	PF00069	0.504
MOD_Plk_4	120	126	PF00069	0.612
MOD_Plk_4	129	135	PF00069	0.454
MOD_Plk_4	160	166	PF00069	0.495
MOD_Plk_4	211	217	PF00069	0.500
MOD_Plk_4	248	254	PF00069	0.615
MOD_Plk_4	421	427	PF00069	0.504
MOD_Plk_4	480	486	PF00069	0.528
MOD_ProDKin_1	11	17	PF00069	0.816
MOD_ProDKin_1	19	25	PF00069	0.762
MOD_ProDKin_1	277	283	PF00069	0.504
MOD_ProDKin_1	29	35	PF00069	0.740
MOD_ProDKin_1	660	666	PF00069	0.435
MOD_SUMO_rev_2	616	622	PF00179	0.517
TRG_DiLeu_BaEn_1	256	261	PF01217	0.515
TRG_ENDOCYTIC_2	134	137	PF00928	0.448
TRG_ENDOCYTIC_2	165	168	PF00928	0.401
TRG_ENDOCYTIC_2	282	285	PF00928	0.504
TRG_ENDOCYTIC_2	337	340	PF00928	0.504
TRG_ENDOCYTIC_2	591	594	PF00928	0.504
TRG_ENDOCYTIC_2	603	606	PF00928	0.504
TRG_ER_diArg_1	149	151	PF00400	0.647
TRG_ER_diArg_1	3	5	PF00400	0.785
TRG_ER_diArg_1	326	329	PF00400	0.515
TRG_ER_diArg_1	670	672	PF00400	0.590
TRG_NLS_MonoCore_2	567	572	PF00514	0.515
TRG_NLS_MonoCore_2	668	673	PF00514	0.609
TRG_Pf-PMV_PEXEL_1	141	145	PF00026	0.620
TRG_Pf-PMV_PEXEL_1	416	420	PF00026	0.328
TRG_Pf-PMV_PEXEL_1	514	519	PF00026	0.410

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IAY9	Leptomonas seymouri	74%	100%
A0A0S4JP31	Bodo saltans	65%	100%
A0A1X0NMZ2	Trypanosomatidae	71%	100%
A0A3S5H6P5	Leishmania donovani	95%	100%
A0A422NLD0	Trypanosoma rangeli	66%	100%
A2XD35	Oryza sativa subsp. indica	24%	100%
A4H7A3	Leishmania braziliensis	87%	100%
A4HVP7	Leishmania infantum	95%	100%
A7LBL2	Leishmania donovani	95%	100%
A9TIK2	Physcomitrium patens	24%	100%
C5WVW2	Sorghum bicolor	24%	100%
D0A6J7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
O24396	Triticum aestivum	23%	100%
O24578	Zea mays	24%	100%
Q017T9	Ostreococcus tauri	23%	100%
Q10R17	Oryza sativa subsp. japonica	24%	100%
Q386E7	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	68%	100%
Q4CWX1	Trypanosoma cruzi (strain CL Brener)	70%	100%
Q4DLT6	Trypanosoma cruzi (strain CL Brener)	71%	100%
Q4QG35	Leishmania major	93%	100%
Q851S8	Oryza sativa subsp. japonica	22%	100%
Q96529	Arabidopsis thaliana	23%	100%
V5BBP4	Trypanosoma cruzi	71%	100%