Putative N(2), N(2)-dimethylguanosine tRNA methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

N(2), N(2)-dimethylguanosine tRNA methyltransferase, putative

Species:

Leishmania mexicana

UniProt:

E9APB8_LEIMU

TriTrypDb:

LmxM.13.0940

Length:

725

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

E9APB8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9APB8

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	1
GO:0002940	tRNA N2-guanine methylation	6	1
GO:0006139	nucleobase-containing compound metabolic process	3	1
GO:0006396	RNA processing	6	1
GO:0006399	tRNA metabolic process	7	1
GO:0006400	tRNA modification	6	1
GO:0006725	cellular aromatic compound metabolic process	3	1
GO:0006807	nitrogen compound metabolic process	2	1
GO:0008033	tRNA processing	8	1
GO:0008152	metabolic process	1	1
GO:0009451	RNA modification	5	1
GO:0009987	cellular process	1	1
GO:0016070	RNA metabolic process	5	1
GO:0030488	tRNA methylation	5	1
GO:0032259	methylation	2	1
GO:0034470	ncRNA processing	7	1
GO:0034641	cellular nitrogen compound metabolic process	3	1
GO:0034660	ncRNA metabolic process	6	1
GO:0043170	macromolecule metabolic process	3	1
GO:0043412	macromolecule modification	4	1
GO:0043414	macromolecule methylation	3	1
GO:0044237	cellular metabolic process	2	1
GO:0044238	primary metabolic process	2	1
GO:0044260	obsolete cellular macromolecule metabolic process	3	1
GO:0046483	heterocycle metabolic process	3	1
GO:0071704	organic substance metabolic process	2	1
GO:0090304	nucleic acid metabolic process	4	1
GO:1901360	organic cyclic compound metabolic process	3	1

Molecular functions
Term	Name	Level	Count
GO:0000049	tRNA binding	5	12
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0004809	tRNA (guanine-N2-)-methyltransferase activity	6	12
GO:0005488	binding	1	12
GO:0008168	methyltransferase activity	4	12
GO:0008170	N-methyltransferase activity	5	12
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	12
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	12
GO:0016423	tRNA (guanine) methyltransferase activity	6	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	280	284	PF00656	0.266
CLV_C14_Caspase3-7	640	644	PF00656	0.583
CLV_NRD_NRD_1	395	397	PF00675	0.510
CLV_NRD_NRD_1	406	408	PF00675	0.520
CLV_NRD_NRD_1	463	465	PF00675	0.682
CLV_NRD_NRD_1	698	700	PF00675	0.564
CLV_NRD_NRD_1	97	99	PF00675	0.286
CLV_PCSK_FUR_1	396	400	PF00082	0.574
CLV_PCSK_FUR_1	404	408	PF00082	0.567
CLV_PCSK_KEX2_1	223	225	PF00082	0.568
CLV_PCSK_KEX2_1	394	396	PF00082	0.276
CLV_PCSK_KEX2_1	398	400	PF00082	0.512
CLV_PCSK_KEX2_1	406	408	PF00082	0.512
CLV_PCSK_KEX2_1	444	446	PF00082	0.833
CLV_PCSK_KEX2_1	697	699	PF00082	0.549
CLV_PCSK_KEX2_1	96	98	PF00082	0.302
CLV_PCSK_PC1ET2_1	223	225	PF00082	0.539
CLV_PCSK_PC1ET2_1	398	400	PF00082	0.412
CLV_PCSK_PC1ET2_1	444	446	PF00082	0.834
CLV_PCSK_PC7_1	391	397	PF00082	0.421
CLV_PCSK_PC7_1	440	446	PF00082	0.676
CLV_PCSK_SKI1_1	120	124	PF00082	0.348
CLV_PCSK_SKI1_1	241	245	PF00082	0.470
CLV_PCSK_SKI1_1	269	273	PF00082	0.300
CLV_PCSK_SKI1_1	315	319	PF00082	0.266
CLV_PCSK_SKI1_1	345	349	PF00082	0.337
CLV_PCSK_SKI1_1	554	558	PF00082	0.358
CLV_PCSK_SKI1_1	618	622	PF00082	0.300
CLV_PCSK_SKI1_1	657	661	PF00082	0.564
DEG_APCC_DBOX_1	314	322	PF00400	0.281
DEG_APCC_DBOX_1	553	561	PF00400	0.411
DEG_SCF_FBW7_2	659	666	PF00400	0.500
DOC_CKS1_1	581	586	PF01111	0.360
DOC_CKS1_1	660	665	PF01111	0.497
DOC_CYCLIN_RxL_1	310	322	PF00134	0.266
DOC_CYCLIN_RxL_1	654	662	PF00134	0.539
DOC_CYCLIN_yCln2_LP_2	512	518	PF00134	0.325
DOC_MAPK_DCC_7	330	338	PF00069	0.269
DOC_MAPK_DCC_7	608	617	PF00069	0.277
DOC_MAPK_gen_1	269	279	PF00069	0.281
DOC_MAPK_gen_1	327	335	PF00069	0.266
DOC_MAPK_gen_1	349	359	PF00069	0.210
DOC_MAPK_MEF2A_6	330	338	PF00069	0.269
DOC_MAPK_MEF2A_6	548	557	PF00069	0.277
DOC_MAPK_MEF2A_6	608	617	PF00069	0.277
DOC_PP2B_LxvP_1	32	35	PF13499	0.428
DOC_PP4_FxxP_1	331	334	PF00568	0.393
DOC_PP4_FxxP_1	68	71	PF00568	0.266
DOC_USP7_MATH_1	187	191	PF00917	0.492
DOC_USP7_MATH_1	214	218	PF00917	0.578
DOC_USP7_MATH_1	232	236	PF00917	0.681
DOC_USP7_MATH_1	256	260	PF00917	0.316
DOC_USP7_MATH_1	387	391	PF00917	0.331
DOC_USP7_MATH_1	413	417	PF00917	0.559
DOC_USP7_MATH_1	430	434	PF00917	0.775
DOC_USP7_MATH_1	470	474	PF00917	0.594
DOC_USP7_MATH_1	642	646	PF00917	0.684
DOC_USP7_MATH_1	650	654	PF00917	0.604
DOC_WW_Pin1_4	14	19	PF00397	0.515
DOC_WW_Pin1_4	185	190	PF00397	0.689
DOC_WW_Pin1_4	234	239	PF00397	0.620
DOC_WW_Pin1_4	258	263	PF00397	0.314
DOC_WW_Pin1_4	468	473	PF00397	0.571
DOC_WW_Pin1_4	478	483	PF00397	0.480
DOC_WW_Pin1_4	580	585	PF00397	0.360
DOC_WW_Pin1_4	659	664	PF00397	0.442
LIG_14-3-3_CanoR_1	379	389	PF00244	0.411
LIG_14-3-3_CanoR_1	537	543	PF00244	0.397
LIG_14-3-3_CanoR_1	89	95	PF00244	0.393
LIG_Actin_WH2_2	101	119	PF00022	0.281
LIG_Actin_WH2_2	76	93	PF00022	0.331
LIG_APCC_ABBA_1	336	341	PF00400	0.266
LIG_BIR_II_1	1	5	PF00653	0.596
LIG_BRCT_BRCA1_1	4	8	PF00533	0.473
LIG_FAT_LD_1	527	535	PF03623	0.355
LIG_FHA_1	235	241	PF00498	0.565
LIG_FHA_1	26	32	PF00498	0.412
LIG_FHA_1	548	554	PF00498	0.369
LIG_FHA_1	581	587	PF00498	0.325
LIG_FHA_2	104	110	PF00498	0.266
LIG_FHA_2	157	163	PF00498	0.416
LIG_FHA_2	278	284	PF00498	0.266
LIG_FHA_2	623	629	PF00498	0.313
LIG_LIR_Apic_2	374	380	PF02991	0.331
LIG_LIR_Gen_1	521	532	PF02991	0.167
LIG_LIR_Gen_1	574	581	PF02991	0.267
LIG_LIR_Gen_1	85	95	PF02991	0.325
LIG_LIR_Nem_3	265	271	PF02991	0.381
LIG_LIR_Nem_3	521	527	PF02991	0.167
LIG_LIR_Nem_3	574	578	PF02991	0.306
LIG_LIR_Nem_3	85	90	PF02991	0.325
LIG_LYPXL_yS_3	157	160	PF13949	0.544
LIG_MYND_1	238	242	PF01753	0.456
LIG_NRBOX	526	532	PF00104	0.266
LIG_PCNA_yPIPBox_3	241	252	PF02747	0.329
LIG_PTB_Apo_2	124	131	PF02174	0.411
LIG_PTB_Apo_2	144	151	PF02174	0.411
LIG_PTB_Phospho_1	124	130	PF10480	0.411
LIG_PTB_Phospho_1	144	150	PF10480	0.411
LIG_REV1ctd_RIR_1	345	353	PF16727	0.325
LIG_SH2_CRK	10	14	PF00017	0.553
LIG_SH2_CRK	150	154	PF00017	0.411
LIG_SH2_CRK	351	355	PF00017	0.281
LIG_SH2_CRK	517	521	PF00017	0.281
LIG_SH2_NCK_1	10	14	PF00017	0.514
LIG_SH2_NCK_1	517	521	PF00017	0.266
LIG_SH2_NCK_1	69	73	PF00017	0.266
LIG_SH2_PTP2	575	578	PF00017	0.411
LIG_SH2_SRC	130	133	PF00017	0.325
LIG_SH2_STAT3	366	369	PF00017	0.296
LIG_SH2_STAT5	121	124	PF00017	0.285
LIG_SH2_STAT5	130	133	PF00017	0.343
LIG_SH2_STAT5	343	346	PF00017	0.266
LIG_SH2_STAT5	567	570	PF00017	0.434
LIG_SH2_STAT5	575	578	PF00017	0.386
LIG_SH3_1	686	692	PF00018	0.440
LIG_SH3_2	469	474	PF14604	0.514
LIG_SH3_3	152	158	PF00018	0.505
LIG_SH3_3	225	231	PF00018	0.584
LIG_SH3_3	28	34	PF00018	0.389
LIG_SH3_3	466	472	PF00018	0.512
LIG_SH3_3	558	564	PF00018	0.294
LIG_SH3_3	575	581	PF00018	0.199
LIG_SH3_3	686	692	PF00018	0.440
LIG_SUMO_SIM_anti_2	103	109	PF11976	0.360
LIG_SUMO_SIM_par_1	250	255	PF11976	0.272
LIG_SUMO_SIM_par_1	657	662	PF11976	0.288
LIG_SUMO_SIM_par_1	82	88	PF11976	0.331
LIG_TRAF2_1	390	393	PF00917	0.287
LIG_TRAF2_1	408	411	PF00917	0.384
LIG_TRAF2_1	54	57	PF00917	0.592
LIG_TRAF2_1	556	559	PF00917	0.281
LIG_TRFH_1	566	570	PF08558	0.331
LIG_TYR_ITIM	155	160	PF00017	0.451
LIG_UBA3_1	616	624	PF00899	0.337
MOD_CDC14_SPxK_1	471	474	PF00782	0.657
MOD_CDK_SPxK_1	468	474	PF00069	0.602
MOD_CDK_SPxxK_3	234	241	PF00069	0.455
MOD_CK1_1	2	8	PF00069	0.624
MOD_CK1_1	281	287	PF00069	0.280
MOD_CK1_1	371	377	PF00069	0.474
MOD_CK1_1	423	429	PF00069	0.752
MOD_CK1_1	473	479	PF00069	0.582
MOD_CK1_1	490	496	PF00069	0.533
MOD_CK1_1	51	57	PF00069	0.677
MOD_CK2_1	103	109	PF00069	0.266
MOD_CK2_1	156	162	PF00069	0.409
MOD_CK2_1	387	393	PF00069	0.364
MOD_CK2_1	413	419	PF00069	0.495
MOD_CK2_1	51	57	PF00069	0.684
MOD_CK2_1	522	528	PF00069	0.167
MOD_Cter_Amidation	221	224	PF01082	0.583
MOD_GlcNHglycan	111	114	PF01048	0.266
MOD_GlcNHglycan	233	237	PF01048	0.722
MOD_GlcNHglycan	258	261	PF01048	0.298
MOD_GlcNHglycan	283	286	PF01048	0.322
MOD_GlcNHglycan	359	362	PF01048	0.421
MOD_GlcNHglycan	373	376	PF01048	0.423
MOD_GlcNHglycan	4	7	PF01048	0.476
MOD_GlcNHglycan	415	418	PF01048	0.574
MOD_GlcNHglycan	43	46	PF01048	0.638
MOD_GlcNHglycan	489	492	PF01048	0.596
MOD_GlcNHglycan	512	515	PF01048	0.273
MOD_GSK3_1	22	29	PF00069	0.526
MOD_GSK3_1	232	239	PF00069	0.571
MOD_GSK3_1	277	284	PF00069	0.314
MOD_GSK3_1	37	44	PF00069	0.581
MOD_GSK3_1	490	497	PF00069	0.559
MOD_GSK3_1	506	513	PF00069	0.299
MOD_GSK3_1	518	525	PF00069	0.227
MOD_GSK3_1	622	629	PF00069	0.550
MOD_LATS_1	296	302	PF00433	0.266
MOD_N-GLC_1	413	418	PF02516	0.483
MOD_N-GLC_1	51	56	PF02516	0.707
MOD_N-GLC_1	522	527	PF02516	0.240
MOD_NEK2_1	111	116	PF00069	0.280
MOD_NEK2_1	277	282	PF00069	0.279
MOD_NEK2_1	291	296	PF00069	0.266
MOD_NEK2_1	368	373	PF00069	0.333
MOD_NEK2_2	334	339	PF00069	0.266
MOD_OGLYCOS	580	585	PF02709	0.167
MOD_PIKK_1	518	524	PF00454	0.281
MOD_PIKK_1	595	601	PF00454	0.266
MOD_PK_1	77	83	PF00069	0.266
MOD_PKA_1	398	404	PF00069	0.395
MOD_PKA_2	387	393	PF00069	0.373
MOD_PKA_2	398	404	PF00069	0.620
MOD_PKA_2	473	479	PF00069	0.615
MOD_PKA_2	536	542	PF00069	0.353
MOD_PKA_2	547	553	PF00069	0.295
MOD_PKA_2	595	601	PF00069	0.281
MOD_PKA_2	679	685	PF00069	0.556
MOD_PKA_2	90	96	PF00069	0.337
MOD_PKB_1	577	585	PF00069	0.167
MOD_PKB_1	96	104	PF00069	0.411
MOD_Plk_1	522	528	PF00069	0.240
MOD_Plk_1	667	673	PF00069	0.372
MOD_Plk_1	77	83	PF00069	0.276
MOD_Plk_2-3	103	109	PF00069	0.360
MOD_Plk_4	156	162	PF00069	0.445
MOD_Plk_4	506	512	PF00069	0.356
MOD_Plk_4	571	577	PF00069	0.266
MOD_ProDKin_1	14	20	PF00069	0.516
MOD_ProDKin_1	185	191	PF00069	0.675
MOD_ProDKin_1	234	240	PF00069	0.613
MOD_ProDKin_1	258	264	PF00069	0.314
MOD_ProDKin_1	468	474	PF00069	0.575
MOD_ProDKin_1	478	484	PF00069	0.483
MOD_ProDKin_1	580	586	PF00069	0.360
MOD_ProDKin_1	659	665	PF00069	0.448
MOD_SUMO_rev_2	415	423	PF00179	0.708
TRG_DiLeu_BaLyEn_6	239	244	PF01217	0.436
TRG_DiLeu_BaLyEn_6	331	336	PF01217	0.302
TRG_DiLeu_BaLyEn_6	655	660	PF01217	0.359
TRG_ENDOCYTIC_2	10	13	PF00928	0.551
TRG_ENDOCYTIC_2	150	153	PF00928	0.411
TRG_ENDOCYTIC_2	157	160	PF00928	0.544
TRG_ENDOCYTIC_2	299	302	PF00928	0.266
TRG_ENDOCYTIC_2	575	578	PF00928	0.411
TRG_ER_diArg_1	394	396	PF00400	0.360
TRG_ER_diArg_1	404	407	PF00400	0.583
TRG_ER_diArg_1	576	579	PF00400	0.264
TRG_ER_diArg_1	677	680	PF00400	0.537
TRG_ER_diArg_1	696	699	PF00400	0.685
TRG_ER_diArg_1	89	92	PF00400	0.283
TRG_ER_diArg_1	95	98	PF00400	0.279
TRG_NLS_MonoExtC_3	645	650	PF00514	0.642
TRG_Pf-PMV_PEXEL_1	241	245	PF00026	0.435
TRG_Pf-PMV_PEXEL_1	269	273	PF00026	0.300

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PF43	Leptomonas seymouri	71%	99%
A0A0S4IT52	Bodo saltans	47%	100%
A0A1X0NNC1	Trypanosomatidae	49%	96%
A0A3R7KAT5	Trypanosoma rangeli	52%	100%
A0A3S7WSL8	Leishmania donovani	91%	100%
A4H782	Leishmania braziliensis	81%	100%
A4HVM2	Leishmania infantum	91%	100%
D0A6N2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	50%	100%
Q4QG60	Leishmania major	90%	100%
V5BB27	Trypanosoma cruzi	52%	100%