Calcium uniporter protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Protein of unknown function, DUF607, putative

Species:

Leishmania mexicana

UniProt:

E9AP84_LEIMU

TriTrypDb:

LmxM.13.0600

Length:

335

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0005743	mitochondrial inner membrane	5	6
GO:0016020	membrane	2	6
GO:0019866	organelle inner membrane	4	6
GO:0031090	organelle membrane	3	6
GO:0031966	mitochondrial membrane	4	6
GO:0110165	cellular anatomical entity	1	6
GO:0032991	protein-containing complex	1	1
GO:0034702	monoatomic ion channel complex	4	1
GO:0034703	cation channel complex	5	1
GO:0034704	calcium channel complex	6	1
GO:0098796	membrane protein complex	2	1
GO:0098798	mitochondrial protein-containing complex	2	1
GO:0098800	inner mitochondrial membrane protein complex	3	1
GO:1902495	transmembrane transporter complex	3	1
GO:1990246	uniplex complex	4	1
GO:1990351	transporter complex	2	1

Expansion

Sequence features

E9AP84

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP84

Function

Biological processes
Term	Name	Level	Count
GO:0006873	intracellular monoatomic ion homeostasis	4	7
GO:0006874	intracellular calcium ion homeostasis	7	7
GO:0006875	obsolete intracellular metal ion homeostasis	6	7
GO:0009987	cellular process	1	7
GO:0019725	cellular homeostasis	2	7
GO:0030003	intracellular monoatomic cation homeostasis	5	7
GO:0042592	homeostatic process	3	7
GO:0048878	chemical homeostasis	4	7
GO:0050801	monoatomic ion homeostasis	5	7
GO:0051560	mitochondrial calcium ion homeostasis	8	7
GO:0055065	obsolete metal ion homeostasis	7	7
GO:0055074	calcium ion homeostasis	8	7
GO:0055080	monoatomic cation homeostasis	6	7
GO:0055082	intracellular chemical homeostasis	3	7
GO:0065007	biological regulation	1	7
GO:0065008	regulation of biological quality	2	7
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	7
GO:0072507	obsolete divalent inorganic cation homeostasis	7	7
GO:0098771	inorganic ion homeostasis	6	7
GO:0006810	transport	3	1
GO:0006811	monoatomic ion transport	4	1
GO:0006812	monoatomic cation transport	5	1
GO:0006816	calcium ion transport	7	1
GO:0006851	mitochondrial calcium ion transmembrane transport	4	1
GO:0030001	metal ion transport	6	1
GO:0034220	monoatomic ion transmembrane transport	3	1
GO:0036444	calcium import into the mitochondrion	5	1
GO:0051179	localization	1	1
GO:0051234	establishment of localization	2	1
GO:0055085	transmembrane transport	2	1
GO:0070588	calcium ion transmembrane transport	6	1
GO:0098655	monoatomic cation transmembrane transport	4	1
GO:0098660	inorganic ion transmembrane transport	4	1
GO:0098662	inorganic cation transmembrane transport	5	1
GO:1990542	mitochondrial transmembrane transport	3	1

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	7
GO:0005216	monoatomic ion channel activity	4	7
GO:0005261	monoatomic cation channel activity	5	7
GO:0005262	calcium channel activity	6	7
GO:0008324	monoatomic cation transmembrane transporter activity	4	7
GO:0015075	monoatomic ion transmembrane transporter activity	3	7
GO:0015085	calcium ion transmembrane transporter activity	6	7
GO:0015267	channel activity	4	7
GO:0015291	secondary active transmembrane transporter activity	4	7
GO:0015292	uniporter activity	5	7
GO:0015318	inorganic molecular entity transmembrane transporter activity	3	7
GO:0022803	passive transmembrane transporter activity	3	7
GO:0022804	active transmembrane transporter activity	3	7
GO:0022857	transmembrane transporter activity	2	7
GO:0022890	inorganic cation transmembrane transporter activity	4	7
GO:0046873	metal ion transmembrane transporter activity	5	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	77	81	PF00656	0.786
CLV_NRD_NRD_1	177	179	PF00675	0.251
CLV_NRD_NRD_1	261	263	PF00675	0.269
CLV_NRD_NRD_1	283	285	PF00675	0.375
CLV_NRD_NRD_1	315	317	PF00675	0.417
CLV_PCSK_KEX2_1	169	171	PF00082	0.198
CLV_PCSK_KEX2_1	283	285	PF00082	0.375
CLV_PCSK_KEX2_1	315	317	PF00082	0.417
CLV_PCSK_PC1ET2_1	169	171	PF00082	0.198
CLV_PCSK_PC7_1	311	317	PF00082	0.356
CLV_PCSK_SKI1_1	178	182	PF00082	0.232
CLV_PCSK_SKI1_1	283	287	PF00082	0.380
DEG_MDM2_SWIB_1	215	222	PF02201	0.269
DOC_MAPK_gen_1	143	152	PF00069	0.534
DOC_USP7_MATH_1	129	133	PF00917	0.662
DOC_USP7_MATH_1	36	40	PF00917	0.653
DOC_USP7_MATH_1	90	94	PF00917	0.655
DOC_USP7_UBL2_3	263	267	PF12436	0.511
DOC_WW_Pin1_4	127	132	PF00397	0.744
DOC_WW_Pin1_4	20	25	PF00397	0.671
DOC_WW_Pin1_4	44	49	PF00397	0.725
DOC_WW_Pin1_4	88	93	PF00397	0.691
LIG_14-3-3_CanoR_1	111	117	PF00244	0.624
LIG_14-3-3_CanoR_1	283	288	PF00244	0.668
LIG_14-3-3_CanoR_1	294	298	PF00244	0.557
LIG_Actin_WH2_2	179	197	PF00022	0.516
LIG_Actin_WH2_2	98	113	PF00022	0.617
LIG_FHA_1	202	208	PF00498	0.340
LIG_FHA_1	224	230	PF00498	0.269
LIG_FHA_2	155	161	PF00498	0.469
LIG_FHA_2	284	290	PF00498	0.487
LIG_LIR_Gen_1	198	207	PF02991	0.284
LIG_LIR_Gen_1	216	227	PF02991	0.334
LIG_LIR_Gen_1	232	242	PF02991	0.301
LIG_LIR_LC3C_4	204	208	PF02991	0.248
LIG_LIR_Nem_3	198	202	PF02991	0.284
LIG_LIR_Nem_3	216	222	PF02991	0.334
LIG_LIR_Nem_3	225	231	PF02991	0.339
LIG_LIR_Nem_3	232	238	PF02991	0.398
LIG_LIR_Nem_3	250	255	PF02991	0.467
LIG_LIR_Nem_3	55	60	PF02991	0.770
LIG_NRBOX	160	166	PF00104	0.469
LIG_Pex14_1	252	256	PF04695	0.516
LIG_Pex14_2	210	214	PF04695	0.283
LIG_Pex14_2	215	219	PF04695	0.288
LIG_PTAP_UEV_1	1	6	PF05743	0.611
LIG_PTB_Apo_2	208	215	PF02174	0.323
LIG_SH2_CRK	231	235	PF00017	0.304
LIG_SH2_CRK	57	61	PF00017	0.666
LIG_SH2_GRB2like	209	212	PF00017	0.283
LIG_SH2_NCK_1	268	272	PF00017	0.484
LIG_SH2_STAT5	190	193	PF00017	0.485
LIG_SH2_STAT5	209	212	PF00017	0.396
LIG_SH2_STAT5	227	230	PF00017	0.304
LIG_SH2_STAT5	231	234	PF00017	0.304
LIG_SH3_3	120	126	PF00018	0.652
LIG_SH3_3	128	134	PF00018	0.654
LIG_SH3_3	186	192	PF00018	0.516
LIG_SH3_3	21	27	PF00018	0.668
LIG_SH3_3	28	34	PF00018	0.668
LIG_TRAF2_1	101	104	PF00917	0.497
LIG_TYR_ITIM	229	234	PF00017	0.304
LIG_WRC_WIRS_1	196	201	PF05994	0.284
LIG_WRC_WIRS_1	214	219	PF05994	0.255
MOD_CK1_1	113	119	PF00069	0.630
MOD_CK1_1	130	136	PF00069	0.674
MOD_CK1_1	141	147	PF00069	0.608
MOD_CK1_1	39	45	PF00069	0.684
MOD_CK2_1	154	160	PF00069	0.442
MOD_CK2_1	283	289	PF00069	0.486
MOD_Cter_Amidation	260	263	PF01082	0.198
MOD_GlcNHglycan	138	141	PF01048	0.471
MOD_GlcNHglycan	143	146	PF01048	0.416
MOD_GlcNHglycan	2	5	PF01048	0.535
MOD_GlcNHglycan	245	248	PF01048	0.355
MOD_GlcNHglycan	38	41	PF01048	0.457
MOD_GlcNHglycan	48	51	PF01048	0.496
MOD_GSK3_1	168	175	PF00069	0.435
MOD_GSK3_1	243	250	PF00069	0.577
MOD_GSK3_1	35	42	PF00069	0.664
MOD_GSK3_1	86	93	PF00069	0.684
MOD_N-GLC_1	247	252	PF02516	0.198
MOD_N-GLC_1	8	13	PF02516	0.404
MOD_NEK2_1	136	141	PF00069	0.744
MOD_NEK2_1	201	206	PF00069	0.205
MOD_NEK2_1	229	234	PF00069	0.310
MOD_NEK2_1	53	58	PF00069	0.693
MOD_NEK2_2	154	159	PF00069	0.469
MOD_PIKK_1	138	144	PF00454	0.569
MOD_PIKK_1	201	207	PF00454	0.248
MOD_PKA_1	283	289	PF00069	0.548
MOD_PKA_2	110	116	PF00069	0.620
MOD_PKA_2	243	249	PF00069	0.453
MOD_PKA_2	283	289	PF00069	0.548
MOD_PKA_2	293	299	PF00069	0.566
MOD_Plk_1	113	119	PF00069	0.599
MOD_Plk_1	154	160	PF00069	0.469
MOD_Plk_4	223	229	PF00069	0.280
MOD_Plk_4	234	240	PF00069	0.422
MOD_Plk_4	283	289	PF00069	0.462
MOD_ProDKin_1	127	133	PF00069	0.748
MOD_ProDKin_1	20	26	PF00069	0.672
MOD_ProDKin_1	44	50	PF00069	0.721
MOD_ProDKin_1	88	94	PF00069	0.688
TRG_DiLeu_BaEn_1	160	165	PF01217	0.469
TRG_DiLeu_BaLyEn_6	189	194	PF01217	0.455
TRG_ENDOCYTIC_2	190	193	PF00928	0.469
TRG_ENDOCYTIC_2	196	199	PF00928	0.304
TRG_ENDOCYTIC_2	231	234	PF00928	0.304
TRG_ENDOCYTIC_2	57	60	PF00928	0.696
TRG_ER_diArg_1	283	285	PF00400	0.575
TRG_NLS_MonoExtC_3	177	183	PF00514	0.445

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PCI6	Leptomonas seymouri	46%	100%
A0A3S7WSE8	Leishmania donovani	85%	100%
A4H749	Leishmania braziliensis	72%	100%
A4HVI9	Leishmania infantum	86%	100%
Q4QG94	Leishmania major	83%	100%