Putative flagellar radial spoke protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Flagellar radial spoke protein 4/6

Species:

Leishmania mexicana

UniProt:

E9AP67_LEIMU

TriTrypDb:

LmxM.13.0430

Length:

596

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0001534	radial spoke	2	12
GO:0005929	cilium	4	12
GO:0031514	motile cilium	5	12
GO:0032991	protein-containing complex	1	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12
GO:0005930	axoneme	2	1
GO:0097730	non-motile cilium	5	1

Expansion

Sequence features

E9AP67

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP67

Function

Biological processes
Term	Name	Level	Count
GO:0003341	cilium movement	4	12
GO:0006996	organelle organization	4	12
GO:0007017	microtubule-based process	2	12
GO:0007018	microtubule-based movement	3	12
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0022607	cellular component assembly	4	12
GO:0030030	cell projection organization	4	11
GO:0030031	cell projection assembly	5	11
GO:0044782	cilium organization	5	11
GO:0060271	cilium assembly	6	11
GO:0060294	cilium movement involved in cell motility	5	12
GO:0070925	organelle assembly	5	11
GO:0071840	cellular component organization or biogenesis	2	12
GO:0120031	plasma membrane bounded cell projection assembly	6	11
GO:0120036	plasma membrane bounded cell projection organization	5	11
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0001578	microtubule bundle formation	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0035082	axoneme assembly	5	1

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	193	195	PF00675	0.289
CLV_NRD_NRD_1	213	215	PF00675	0.117
CLV_NRD_NRD_1	327	329	PF00675	0.334
CLV_NRD_NRD_1	525	527	PF00675	0.237
CLV_NRD_NRD_1	573	575	PF00675	0.557
CLV_NRD_NRD_1	91	93	PF00675	0.351
CLV_PCSK_FUR_1	191	195	PF00082	0.334
CLV_PCSK_KEX2_1	193	195	PF00082	0.273
CLV_PCSK_KEX2_1	263	265	PF00082	0.257
CLV_PCSK_KEX2_1	525	527	PF00082	0.293
CLV_PCSK_KEX2_1	573	575	PF00082	0.557
CLV_PCSK_KEX2_1	91	93	PF00082	0.322
CLV_PCSK_PC1ET2_1	263	265	PF00082	0.260
CLV_PCSK_SKI1_1	160	164	PF00082	0.252
CLV_PCSK_SKI1_1	263	267	PF00082	0.258
CLV_PCSK_SKI1_1	331	335	PF00082	0.247
CLV_PCSK_SKI1_1	97	101	PF00082	0.309
DEG_SCF_SKP2-CKS1_1	464	471	PF00560	0.534
DOC_CKS1_1	421	426	PF01111	0.358
DOC_MAPK_gen_1	211	221	PF00069	0.440
DOC_MAPK_gen_1	331	340	PF00069	0.534
DOC_MAPK_gen_1	525	532	PF00069	0.460
DOC_PP2B_LxvP_1	102	105	PF13499	0.565
DOC_PP2B_LxvP_1	128	131	PF13499	0.534
DOC_PP2B_LxvP_1	338	341	PF13499	0.534
DOC_PP4_FxxP_1	100	103	PF00568	0.489
DOC_USP7_MATH_1	239	243	PF00917	0.553
DOC_USP7_MATH_1	550	554	PF00917	0.553
DOC_USP7_UBL2_3	175	179	PF12436	0.523
DOC_USP7_UBL2_3	211	215	PF12436	0.503
DOC_USP7_UBL2_3	375	379	PF12436	0.460
DOC_WW_Pin1_4	237	242	PF00397	0.501
DOC_WW_Pin1_4	249	254	PF00397	0.394
DOC_WW_Pin1_4	339	344	PF00397	0.555
DOC_WW_Pin1_4	420	425	PF00397	0.357
DOC_WW_Pin1_4	465	470	PF00397	0.534
DOC_WW_Pin1_4	541	546	PF00397	0.446
LIG_14-3-3_CanoR_1	232	236	PF00244	0.448
LIG_14-3-3_CanoR_1	292	301	PF00244	0.494
LIG_14-3-3_CanoR_1	525	532	PF00244	0.543
LIG_BIR_II_1	1	5	PF00653	0.573
LIG_BIR_III_4	403	407	PF00653	0.553
LIG_FHA_1	120	126	PF00498	0.443
LIG_FHA_1	199	205	PF00498	0.498
LIG_FHA_1	232	238	PF00498	0.514
LIG_FHA_1	265	271	PF00498	0.473
LIG_FHA_1	490	496	PF00498	0.473
LIG_FHA_1	563	569	PF00498	0.467
LIG_FHA_2	107	113	PF00498	0.448
LIG_FHA_2	181	187	PF00498	0.489
LIG_FHA_2	312	318	PF00498	0.553
LIG_FHA_2	557	563	PF00498	0.496
LIG_FHA_2	69	75	PF00498	0.539
LIG_Integrin_isoDGR_2	370	372	PF01839	0.260
LIG_KLC1_Yacidic_2	200	205	PF13176	0.534
LIG_LIR_Gen_1	115	125	PF02991	0.448
LIG_LIR_Gen_1	132	138	PF02991	0.489
LIG_LIR_Gen_1	242	253	PF02991	0.492
LIG_LIR_Gen_1	31	40	PF02991	0.458
LIG_LIR_Nem_3	115	120	PF02991	0.448
LIG_LIR_Nem_3	132	136	PF02991	0.489
LIG_LIR_Nem_3	186	190	PF02991	0.453
LIG_LIR_Nem_3	224	230	PF02991	0.472
LIG_LIR_Nem_3	242	248	PF02991	0.373
LIG_LIR_Nem_3	31	36	PF02991	0.458
LIG_LIR_Nem_3	332	338	PF02991	0.460
LIG_LIR_Nem_3	420	425	PF02991	0.448
LIG_LIR_Nem_3	428	434	PF02991	0.467
LIG_NRBOX	38	44	PF00104	0.553
LIG_Pex14_1	527	531	PF04695	0.460
LIG_Pex14_2	177	181	PF04695	0.489
LIG_REV1ctd_RIR_1	174	183	PF16727	0.489
LIG_SH2_CRK	117	121	PF00017	0.460
LIG_SH2_CRK	33	37	PF00017	0.460
LIG_SH2_CRK	335	339	PF00017	0.460
LIG_SH2_NCK_1	117	121	PF00017	0.460
LIG_SH2_PTP2	227	230	PF00017	0.553
LIG_SH2_SRC	195	198	PF00017	0.505
LIG_SH2_SRC	429	432	PF00017	0.469
LIG_SH2_STAP1	233	237	PF00017	0.534
LIG_SH2_STAT3	21	24	PF00017	0.520
LIG_SH2_STAT5	117	120	PF00017	0.460
LIG_SH2_STAT5	187	190	PF00017	0.454
LIG_SH2_STAT5	195	198	PF00017	0.439
LIG_SH2_STAT5	203	206	PF00017	0.425
LIG_SH2_STAT5	21	24	PF00017	0.448
LIG_SH2_STAT5	227	230	PF00017	0.543
LIG_SH2_STAT5	233	236	PF00017	0.523
LIG_SH2_STAT5	285	288	PF00017	0.448
LIG_SH2_STAT5	366	369	PF00017	0.460
LIG_SH2_STAT5	429	432	PF00017	0.444
LIG_SH2_STAT5	531	534	PF00017	0.445
LIG_SH3_2	469	474	PF14604	0.534
LIG_SH3_3	324	330	PF00018	0.500
LIG_SH3_3	337	343	PF00018	0.551
LIG_SH3_3	374	380	PF00018	0.509
LIG_SH3_3	394	400	PF00018	0.317
LIG_SH3_3	431	437	PF00018	0.534
LIG_SH3_3	459	465	PF00018	0.476
LIG_SH3_3	466	472	PF00018	0.452
LIG_SH3_3	544	550	PF00018	0.482
LIG_SH3_3	558	564	PF00018	0.406
LIG_SH3_4	211	218	PF00018	0.509
LIG_SH3_5	341	345	PF00018	0.498
LIG_SUMO_SIM_par_1	336	342	PF11976	0.562
LIG_TRAF2_1	151	154	PF00917	0.460
LIG_TRAF2_1	304	307	PF00917	0.460
LIG_TRAF2_1	310	313	PF00917	0.460
LIG_TRAF2_1	413	416	PF00917	0.498
LIG_TRAF2_1	454	457	PF00917	0.502
LIG_TRAF2_1	86	89	PF00917	0.534
LIG_WRC_WIRS_1	61	66	PF05994	0.534
LIG_WW_3	211	215	PF00397	0.526
MOD_CDK_SPxK_1	465	471	PF00069	0.534
MOD_CDK_SPxxK_3	237	244	PF00069	0.553
MOD_CK1_1	132	138	PF00069	0.509
MOD_CK1_1	295	301	PF00069	0.534
MOD_CK1_1	497	503	PF00069	0.381
MOD_CK2_1	106	112	PF00069	0.448
MOD_CK2_1	4	10	PF00069	0.583
MOD_CK2_1	556	562	PF00069	0.534
MOD_CK2_1	580	586	PF00069	0.628
MOD_CK2_1	68	74	PF00069	0.345
MOD_Cter_Amidation	523	526	PF01082	0.260
MOD_GlcNHglycan	269	273	PF01048	0.300
MOD_GlcNHglycan	30	33	PF01048	0.304
MOD_GlcNHglycan	6	9	PF01048	0.554
MOD_GlcNHglycan	76	79	PF01048	0.322
MOD_GSK3_1	115	122	PF00069	0.446
MOD_GSK3_1	231	238	PF00069	0.453
MOD_GSK3_1	264	271	PF00069	0.508
MOD_GSK3_1	291	298	PF00069	0.451
MOD_GSK3_1	60	67	PF00069	0.514
MOD_GSK3_1	68	75	PF00069	0.504
MOD_N-GLC_1	497	502	PF02516	0.260
MOD_NEK2_1	1	6	PF00069	0.615
MOD_NEK2_1	158	163	PF00069	0.448
MOD_NEK2_1	235	240	PF00069	0.465
MOD_NEK2_1	270	275	PF00069	0.498
MOD_NEK2_1	64	69	PF00069	0.497
MOD_NEK2_2	198	203	PF00069	0.498
MOD_NEK2_2	489	494	PF00069	0.473
MOD_PIKK_1	80	86	PF00454	0.553
MOD_PKA_1	525	531	PF00069	0.553
MOD_PKA_2	231	237	PF00069	0.448
MOD_PKA_2	259	265	PF00069	0.553
MOD_PKA_2	291	297	PF00069	0.515
MOD_PKA_2	371	377	PF00069	0.448
MOD_PKA_2	525	531	PF00069	0.553
MOD_Plk_1	158	164	PF00069	0.448
MOD_Plk_1	80	86	PF00069	0.515
MOD_Plk_2-3	106	112	PF00069	0.523
MOD_Plk_2-3	311	317	PF00069	0.553
MOD_Plk_2-3	580	586	PF00069	0.628
MOD_Plk_4	121	127	PF00069	0.523
MOD_Plk_4	198	204	PF00069	0.498
MOD_Plk_4	231	237	PF00069	0.448
MOD_Plk_4	270	276	PF00069	0.418
MOD_Plk_4	60	66	PF00069	0.462
MOD_ProDKin_1	237	243	PF00069	0.501
MOD_ProDKin_1	249	255	PF00069	0.394
MOD_ProDKin_1	339	345	PF00069	0.555
MOD_ProDKin_1	420	426	PF00069	0.357
MOD_ProDKin_1	465	471	PF00069	0.534
MOD_ProDKin_1	541	547	PF00069	0.446
MOD_SUMO_rev_2	168	177	PF00179	0.534
MOD_SUMO_rev_2	89	99	PF00179	0.498
TRG_DiLeu_BaEn_1	153	158	PF01217	0.460
TRG_DiLeu_BaEn_2	59	65	PF01217	0.534
TRG_DiLeu_BaEn_4	153	159	PF01217	0.460
TRG_ENDOCYTIC_2	117	120	PF00928	0.448
TRG_ENDOCYTIC_2	187	190	PF00928	0.441
TRG_ENDOCYTIC_2	227	230	PF00928	0.553
TRG_ENDOCYTIC_2	33	36	PF00928	0.448
TRG_ENDOCYTIC_2	335	338	PF00928	0.460
TRG_ENDOCYTIC_2	431	434	PF00928	0.492
TRG_ER_diArg_1	190	193	PF00400	0.495
TRG_ER_diArg_1	525	527	PF00400	0.440
TRG_ER_diArg_1	572	574	PF00400	0.563
TRG_ER_diArg_1	90	92	PF00400	0.553
TRG_NES_CRM1_1	34	46	PF08389	0.553
TRG_NLS_MonoExtN_4	211	218	PF00514	0.534

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDF4	Leptomonas seymouri	74%	100%
A0A0S4JGY1	Bodo saltans	50%	100%
A0A1X0NN98	Trypanosomatidae	58%	100%
A0A3S7WSD6	Leishmania donovani	94%	99%
A0A422NNZ6	Trypanosoma rangeli	57%	100%
A4H732	Leishmania braziliensis	88%	99%
A4HVG6	Leishmania infantum	95%	100%
D0A6U3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	57%	100%
Q01656	Chlamydomonas reinhardtii	28%	100%
Q4QGB1	Leishmania major	93%	99%
Q9H0K4	Homo sapiens	28%	83%
V5BDZ5	Trypanosoma cruzi	58%	92%