Protein kinase A regulatory subunit

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein kinase A regulatory subunit

Species:

Leishmania mexicana

UniProt:

E9AP50_LEIMU

TriTrypDb:

LmxM.13.0160

Length:

503

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 12

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	12
GO:0042995	cell projection	2	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0110165	cellular anatomical entity	1	12
GO:0120025	plasma membrane bounded cell projection	3	12

Expansion

Sequence features

E9AP50

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP50

Function

Biological processes
Term	Name	Level	Count
GO:0003341	cilium movement	4	1
GO:0007017	microtubule-based process	2	1
GO:0007018	microtubule-based movement	3	1
GO:0009987	cellular process	1	5
GO:0044145	modulation of formation of structure involved in a symbiotic process	3	1
GO:0050789	regulation of biological process	2	1
GO:0060294	cilium movement involved in cell motility	5	1
GO:0065007	biological regulation	1	1
GO:0006793	phosphorus metabolic process	3	4
GO:0006796	phosphate-containing compound metabolic process	4	4
GO:0008152	metabolic process	1	4
GO:0016310	phosphorylation	5	4
GO:0044237	cellular metabolic process	2	4

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	4
GO:0016301	kinase activity	4	4
GO:0016740	transferase activity	2	4
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	122	124	PF00675	0.439
CLV_NRD_NRD_1	203	205	PF00675	0.590
CLV_NRD_NRD_1	351	353	PF00675	0.450
CLV_NRD_NRD_1	460	462	PF00675	0.353
CLV_PCSK_FUR_1	201	205	PF00082	0.657
CLV_PCSK_KEX2_1	203	205	PF00082	0.587
CLV_PCSK_KEX2_1	350	352	PF00082	0.450
CLV_PCSK_KEX2_1	460	462	PF00082	0.353
CLV_PCSK_KEX2_1	475	477	PF00082	0.519
CLV_PCSK_PC1ET2_1	475	477	PF00082	0.496
CLV_PCSK_SKI1_1	123	127	PF00082	0.431
CLV_PCSK_SKI1_1	152	156	PF00082	0.495
CLV_PCSK_SKI1_1	251	255	PF00082	0.508
CLV_PCSK_SKI1_1	53	57	PF00082	0.414
DEG_APCC_DBOX_1	133	141	PF00400	0.573
DEG_APCC_DBOX_1	52	60	PF00400	0.410
DOC_CDC14_PxL_1	359	367	PF14671	0.441
DOC_CKS1_1	20	25	PF01111	0.588
DOC_CKS1_1	315	320	PF01111	0.353
DOC_CYCLIN_RxL_1	120	127	PF00134	0.462
DOC_MAPK_gen_1	201	209	PF00069	0.699
DOC_MAPK_gen_1	350	356	PF00069	0.309
DOC_MAPK_MEF2A_6	350	358	PF00069	0.291
DOC_PP1_RVXF_1	142	148	PF00149	0.460
DOC_PP1_RVXF_1	24	31	PF00149	0.574
DOC_USP7_MATH_1	319	323	PF00917	0.208
DOC_USP7_MATH_1	379	383	PF00917	0.445
DOC_USP7_UBL2_3	294	298	PF12436	0.366
DOC_WW_Pin1_4	19	24	PF00397	0.469
DOC_WW_Pin1_4	3	8	PF00397	0.602
DOC_WW_Pin1_4	314	319	PF00397	0.353
LIG_14-3-3_CanoR_1	123	129	PF00244	0.537
LIG_14-3-3_CanoR_1	203	208	PF00244	0.583
LIG_14-3-3_CanoR_1	476	486	PF00244	0.560
LIG_BIR_II_1	1	5	PF00653	0.595
LIG_BIR_III_4	415	419	PF00653	0.420
LIG_BRCT_BRCA1_1	381	385	PF00533	0.462
LIG_eIF4E_1	120	126	PF01652	0.428
LIG_FHA_1	189	195	PF00498	0.759
LIG_FHA_1	204	210	PF00498	0.600
LIG_FHA_1	230	236	PF00498	0.477
LIG_FHA_1	315	321	PF00498	0.353
LIG_FHA_1	325	331	PF00498	0.353
LIG_FHA_1	406	412	PF00498	0.374
LIG_FHA_1	453	459	PF00498	0.370
LIG_FHA_2	141	147	PF00498	0.456
LIG_FHA_2	253	259	PF00498	0.406
LIG_FHA_2	275	281	PF00498	0.420
LIG_FHA_2	379	385	PF00498	0.514
LIG_FHA_2	441	447	PF00498	0.444
LIG_LIR_Apic_2	312	318	PF02991	0.353
LIG_LIR_Gen_1	35	45	PF02991	0.568
LIG_LIR_Gen_1	353	363	PF02991	0.433
LIG_LIR_Gen_1	369	379	PF02991	0.394
LIG_LIR_Gen_1	397	404	PF02991	0.353
LIG_LIR_Gen_1	484	490	PF02991	0.581
LIG_LIR_Nem_3	338	343	PF02991	0.350
LIG_LIR_Nem_3	35	40	PF02991	0.577
LIG_LIR_Nem_3	397	403	PF02991	0.353
LIG_LIR_Nem_3	430	435	PF02991	0.422
LIG_LIR_Nem_3	480	486	PF02991	0.557
LIG_LIR_Nem_3	66	71	PF02991	0.458
LIG_PCNA_yPIPBox_3	24	34	PF02747	0.513
LIG_PTB_Apo_2	77	84	PF02174	0.602
LIG_Rb_pABgroove_1	309	317	PF01858	0.369
LIG_REV1ctd_RIR_1	28	38	PF16727	0.333
LIG_SH2_CRK	315	319	PF00017	0.353
LIG_SH2_CRK	340	344	PF00017	0.369
LIG_SH2_CRK	486	490	PF00017	0.556
LIG_SH2_NCK_1	315	319	PF00017	0.438
LIG_SH2_PTP2	220	223	PF00017	0.581
LIG_SH2_SRC	220	223	PF00017	0.604
LIG_SH2_SRC	390	393	PF00017	0.387
LIG_SH2_STAT3	283	286	PF00017	0.353
LIG_SH2_STAT3	485	488	PF00017	0.699
LIG_SH2_STAT5	220	223	PF00017	0.522
LIG_SH2_STAT5	283	286	PF00017	0.353
LIG_SH2_STAT5	355	358	PF00017	0.388
LIG_SH2_STAT5	390	393	PF00017	0.369
LIG_SH2_STAT5	402	405	PF00017	0.369
LIG_SH2_STAT5	49	52	PF00017	0.414
LIG_SH3_3	17	23	PF00018	0.462
LIG_SUMO_SIM_anti_2	310	317	PF11976	0.353
LIG_SUMO_SIM_anti_2	408	413	PF11976	0.369
LIG_SUMO_SIM_par_1	136	143	PF11976	0.544
LIG_SUMO_SIM_par_1	252	258	PF11976	0.266
LIG_SUMO_SIM_par_1	38	44	PF11976	0.554
LIG_SUMO_SIM_par_1	446	452	PF11976	0.473
LIG_SUMO_SIM_par_1	54	60	PF11976	0.264
LIG_UBA3_1	244	251	PF00899	0.480
MOD_CDK_SPxxK_3	19	26	PF00069	0.617
MOD_CK1_1	326	332	PF00069	0.286
MOD_CK1_1	33	39	PF00069	0.523
MOD_CK1_1	367	373	PF00069	0.421
MOD_CK1_1	81	87	PF00069	0.606
MOD_CK2_1	252	258	PF00069	0.266
MOD_CK2_1	36	42	PF00069	0.495
MOD_CK2_1	378	384	PF00069	0.440
MOD_Cter_Amidation	121	124	PF01082	0.435
MOD_GlcNHglycan	1	4	PF01048	0.664
MOD_GlcNHglycan	174	177	PF01048	0.749
MOD_GlcNHglycan	286	289	PF01048	0.387
MOD_GlcNHglycan	427	430	PF01048	0.500
MOD_GlcNHglycan	479	482	PF01048	0.668
MOD_GSK3_1	106	113	PF00069	0.561
MOD_GSK3_1	188	195	PF00069	0.691
MOD_GSK3_1	225	232	PF00069	0.512
MOD_GSK3_1	319	326	PF00069	0.473
MOD_GSK3_1	448	455	PF00069	0.353
MOD_GSK3_1	63	70	PF00069	0.283
MOD_N-GLC_1	110	115	PF02516	0.501
MOD_N-GLC_1	418	423	PF02516	0.441
MOD_N-GLC_1	476	481	PF02516	0.608
MOD_N-GLC_1	71	76	PF02516	0.271
MOD_NEK2_1	110	115	PF00069	0.432
MOD_NEK2_1	274	279	PF00069	0.422
MOD_NEK2_1	354	359	PF00069	0.366
MOD_NEK2_2	319	324	PF00069	0.410
MOD_PIKK_1	185	191	PF00454	0.692
MOD_PIKK_1	225	231	PF00454	0.631
MOD_PKA_1	203	209	PF00069	0.573
MOD_PKA_1	475	481	PF00069	0.530
MOD_PKA_2	203	209	PF00069	0.562
MOD_PKA_2	33	39	PF00069	0.322
MOD_PKA_2	336	342	PF00069	0.363
MOD_PKA_2	475	481	PF00069	0.519
MOD_PKB_1	201	209	PF00069	0.635
MOD_Plk_1	192	198	PF00069	0.607
MOD_Plk_2-3	252	258	PF00069	0.266
MOD_Plk_2-3	440	446	PF00069	0.325
MOD_Plk_4	326	332	PF00069	0.286
MOD_Plk_4	354	360	PF00069	0.425
MOD_Plk_4	36	42	PF00069	0.553
MOD_ProDKin_1	19	25	PF00069	0.474
MOD_ProDKin_1	3	9	PF00069	0.596
MOD_ProDKin_1	314	320	PF00069	0.353
MOD_SUMO_for_1	301	304	PF00179	0.353
MOD_SUMO_rev_2	70	78	PF00179	0.273
TRG_DiLeu_BaEn_4	396	402	PF01217	0.369
TRG_ENDOCYTIC_2	340	343	PF00928	0.349
TRG_ENDOCYTIC_2	355	358	PF00928	0.374
TRG_ENDOCYTIC_2	371	374	PF00928	0.397
TRG_ENDOCYTIC_2	486	489	PF00928	0.557
TRG_ER_diArg_1	201	204	PF00400	0.593
TRG_ER_diArg_1	349	352	PF00400	0.447

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P645	Leptomonas seymouri	79%	100%
A0A0S4JEQ3	Bodo saltans	61%	100%
A0A1X0NG29	Trypanosomatidae	24%	92%
A0A1X0NNW8	Trypanosomatidae	67%	100%
A0A3S7WSB4	Leishmania donovani	95%	100%
A0A422NNY9	Trypanosoma rangeli	65%	100%
A4H723	Leishmania braziliensis	86%	100%
A4HVF2	Leishmania infantum	95%	100%
D0A6V7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	63%	100%
O42794	Colletotrichum trifolii	26%	100%
Q4QGD8	Leishmania major	93%	100%
Q5ZM91	Gallus gallus	32%	100%
Q61410	Mus musculus	26%	66%
V5BIC8	Trypanosoma cruzi	66%	100%