Kinesin-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Kinesin-13 2

Species:

Leishmania mexicana

UniProt:

E9AP47_LEIMU

TriTrypDb:

LmxM.13.0130

Length:

726

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005874	microtubule	6	13
GO:0099080	supramolecular complex	2	13
GO:0099081	supramolecular polymer	3	13
GO:0099512	supramolecular fiber	4	13
GO:0099513	polymeric cytoskeletal fiber	5	13
GO:0110165	cellular anatomical entity	1	13
GO:0005737	cytoplasm	2	1
GO:0005929	cilium	4	1
GO:0005930	axoneme	2	1
GO:0042995	cell projection	2	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	1
GO:0097542	ciliary tip	2	1
GO:0120025	plasma membrane bounded cell projection	3	1
GO:0005730	nucleolus	5	1
GO:0005871	kinesin complex	3	1
GO:0005875	microtubule associated complex	2	1
GO:0032991	protein-containing complex	1	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

E9AP47

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: E9AP47

Function

Biological processes
Term	Name	Level	Count
GO:0007017	microtubule-based process	2	16
GO:0007018	microtubule-based movement	3	16
GO:0009987	cellular process	1	16
GO:0000226	microtubule cytoskeleton organization	3	1
GO:0006996	organelle organization	4	1
GO:0007010	cytoskeleton organization	5	1
GO:0007019	microtubule depolymerization	5	1
GO:0010938	cytoplasmic microtubule depolymerization	5	1
GO:0016043	cellular component organization	3	1
GO:0022411	cellular component disassembly	4	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0031109	microtubule polymerization or depolymerization	4	1
GO:0031122	cytoplasmic microtubule organization	4	1
GO:0032984	protein-containing complex disassembly	5	1
GO:0043933	protein-containing complex organization	4	1
GO:0044782	cilium organization	5	1
GO:0051261	protein depolymerization	6	1
GO:0060271	cilium assembly	6	1
GO:0060404	axonemal microtubule depolymerization	6	1
GO:0061523	cilium disassembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0097435	supramolecular fiber organization	4	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1
GO:1903008	organelle disassembly	5	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0003774	cytoskeletal motor activity	1	16
GO:0003777	microtubule motor activity	2	16
GO:0005488	binding	1	16
GO:0005515	protein binding	2	16
GO:0005524	ATP binding	5	16
GO:0008017	microtubule binding	5	16
GO:0008092	cytoskeletal protein binding	3	16
GO:0008270	zinc ion binding	6	11
GO:0015631	tubulin binding	4	16
GO:0017076	purine nucleotide binding	4	16
GO:0030554	adenyl nucleotide binding	5	16
GO:0032553	ribonucleotide binding	3	16
GO:0032555	purine ribonucleotide binding	4	16
GO:0032559	adenyl ribonucleotide binding	5	16
GO:0035639	purine ribonucleoside triphosphate binding	4	16
GO:0036094	small molecule binding	2	16
GO:0043167	ion binding	2	16
GO:0043168	anion binding	3	16
GO:0043169	cation binding	3	11
GO:0046872	metal ion binding	4	11
GO:0046914	transition metal ion binding	5	11
GO:0097159	organic cyclic compound binding	2	16
GO:0097367	carbohydrate derivative binding	2	16
GO:0140657	ATP-dependent activity	1	16
GO:1901265	nucleoside phosphate binding	3	16
GO:1901363	heterocyclic compound binding	2	16
GO:0003824	catalytic activity	1	1
GO:0016462	pyrophosphatase activity	5	1
GO:0016787	hydrolase activity	2	1
GO:0016817	hydrolase activity, acting on acid anhydrides	3	1
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	1
GO:0016887	ATP hydrolysis activity	7	1
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	275	277	PF00675	0.326
CLV_NRD_NRD_1	297	299	PF00675	0.380
CLV_NRD_NRD_1	36	38	PF00675	0.428
CLV_NRD_NRD_1	432	434	PF00675	0.293
CLV_NRD_NRD_1	501	503	PF00675	0.442
CLV_NRD_NRD_1	575	577	PF00675	0.640
CLV_NRD_NRD_1	705	707	PF00675	0.526
CLV_PCSK_KEX2_1	275	277	PF00082	0.326
CLV_PCSK_KEX2_1	299	301	PF00082	0.379
CLV_PCSK_KEX2_1	38	40	PF00082	0.452
CLV_PCSK_KEX2_1	383	385	PF00082	0.642
CLV_PCSK_KEX2_1	432	434	PF00082	0.321
CLV_PCSK_KEX2_1	705	707	PF00082	0.526
CLV_PCSK_PC1ET2_1	299	301	PF00082	0.343
CLV_PCSK_PC1ET2_1	38	40	PF00082	0.410
CLV_PCSK_PC1ET2_1	383	385	PF00082	0.599
CLV_PCSK_PC7_1	379	385	PF00082	0.578
CLV_PCSK_SKI1_1	283	287	PF00082	0.322
CLV_PCSK_SKI1_1	350	354	PF00082	0.480
CLV_PCSK_SKI1_1	477	481	PF00082	0.433
CLV_PCSK_SKI1_1	558	562	PF00082	0.601
DEG_APCC_DBOX_1	137	145	PF00400	0.335
DEG_Nend_UBRbox_1	1	4	PF02207	0.660
DEG_SCF_FBW7_2	72	79	PF00400	0.320
DEG_SPOP_SBC_1	242	246	PF00917	0.265
DEG_SPOP_SBC_1	524	528	PF00917	0.554
DOC_CYCLIN_RxL_1	280	290	PF00134	0.305
DOC_CYCLIN_yClb5_NLxxxL_5	670	679	PF00134	0.438
DOC_CYCLIN_yCln2_LP_2	352	358	PF00134	0.624
DOC_MAPK_gen_1	227	237	PF00069	0.320
DOC_MAPK_gen_1	298	304	PF00069	0.343
DOC_MAPK_MEF2A_6	230	239	PF00069	0.305
DOC_PP1_RVXF_1	250	256	PF00149	0.436
DOC_PP2B_PxIxI_1	29	35	PF00149	0.444
DOC_USP7_MATH_1	12	16	PF00917	0.658
DOC_USP7_MATH_1	242	246	PF00917	0.265
DOC_USP7_MATH_1	28	32	PF00917	0.526
DOC_USP7_MATH_1	565	569	PF00917	0.824
DOC_USP7_MATH_1	570	574	PF00917	0.730
DOC_USP7_MATH_1	604	608	PF00917	0.830
DOC_WW_Pin1_4	19	24	PF00397	0.620
DOC_WW_Pin1_4	2	7	PF00397	0.795
DOC_WW_Pin1_4	26	31	PF00397	0.620
DOC_WW_Pin1_4	327	332	PF00397	0.358
DOC_WW_Pin1_4	408	413	PF00397	0.404
DOC_WW_Pin1_4	520	525	PF00397	0.617
DOC_WW_Pin1_4	608	613	PF00397	0.666
DOC_WW_Pin1_4	72	77	PF00397	0.358
LIG_14-3-3_CanoR_1	13	21	PF00244	0.755
LIG_14-3-3_CanoR_1	227	237	PF00244	0.368
LIG_14-3-3_CanoR_1	252	256	PF00244	0.305
LIG_14-3-3_CanoR_1	293	298	PF00244	0.397
LIG_14-3-3_CanoR_1	320	329	PF00244	0.358
LIG_14-3-3_CanoR_1	525	533	PF00244	0.804
LIG_14-3-3_CanoR_1	569	578	PF00244	0.799
LIG_14-3-3_CanoR_1	603	609	PF00244	0.747
LIG_14-3-3_CanoR_1	718	722	PF00244	0.531
LIG_Actin_WH2_2	202	219	PF00022	0.320
LIG_Actin_WH2_2	666	682	PF00022	0.586
LIG_FHA_1	145	151	PF00498	0.439
LIG_FHA_1	16	22	PF00498	0.747
LIG_FHA_1	194	200	PF00498	0.335
LIG_FHA_1	308	314	PF00498	0.326
LIG_FHA_1	321	327	PF00498	0.358
LIG_FHA_1	509	515	PF00498	0.398
LIG_FHA_1	526	532	PF00498	0.530
LIG_FHA_1	594	600	PF00498	0.650
LIG_FHA_1	710	716	PF00498	0.606
LIG_FHA_1	73	79	PF00498	0.390
LIG_FHA_2	149	155	PF00498	0.488
LIG_FHA_2	252	258	PF00498	0.305
LIG_FHA_2	272	278	PF00498	0.257
LIG_FHA_2	455	461	PF00498	0.510
LIG_FHA_2	533	539	PF00498	0.729
LIG_FHA_2	62	68	PF00498	0.473
LIG_FHA_2	690	696	PF00498	0.467
LIG_FHA_2	92	98	PF00498	0.377
LIG_GBD_Chelix_1	675	683	PF00786	0.573
LIG_LIR_Apic_2	662	667	PF02991	0.598
LIG_LIR_Gen_1	185	195	PF02991	0.377
LIG_LIR_Gen_1	254	259	PF02991	0.387
LIG_LIR_Gen_1	82	92	PF02991	0.324
LIG_LIR_Nem_3	161	166	PF02991	0.330
LIG_LIR_Nem_3	185	190	PF02991	0.320
LIG_LIR_Nem_3	254	258	PF02991	0.387
LIG_LIR_Nem_3	82	88	PF02991	0.357
LIG_NRBOX	169	175	PF00104	0.305
LIG_PCNA_yPIPBox_3	696	708	PF02747	0.510
LIG_SH2_CRK	171	175	PF00017	0.436
LIG_SH2_CRK	187	191	PF00017	0.377
LIG_SH2_CRK	664	668	PF00017	0.534
LIG_SH2_GRB2like	187	190	PF00017	0.436
LIG_SH2_NCK_1	85	89	PF00017	0.436
LIG_SH2_SRC	187	190	PF00017	0.436
LIG_SH2_SRC	85	88	PF00017	0.436
LIG_SH2_STAP1	142	146	PF00017	0.436
LIG_SH2_STAP1	345	349	PF00017	0.358
LIG_SH2_STAP1	693	697	PF00017	0.455
LIG_SH2_STAP1	85	89	PF00017	0.436
LIG_SH2_STAT3	391	394	PF00017	0.334
LIG_SH2_STAT5	391	394	PF00017	0.385
LIG_SH2_STAT5	417	420	PF00017	0.451
LIG_SH2_STAT5	470	473	PF00017	0.479
LIG_SH3_1	37	43	PF00018	0.450
LIG_SH3_2	40	45	PF14604	0.490
LIG_SH3_2	598	603	PF14604	0.554
LIG_SH3_3	20	26	PF00018	0.618
LIG_SH3_3	352	358	PF00018	0.687
LIG_SH3_3	37	43	PF00018	0.450
LIG_SH3_3	489	495	PF00018	0.505
LIG_SH3_3	511	517	PF00018	0.485
LIG_SH3_3	595	601	PF00018	0.569
LIG_SH3_3	62	68	PF00018	0.457
LIG_SH3_3	654	660	PF00018	0.526
LIG_SUMO_SIM_par_1	284	290	PF11976	0.305
LIG_TRAF2_1	457	460	PF00917	0.501
LIG_TRAF2_1	94	97	PF00917	0.335
LIG_TRAF2_2	449	454	PF00917	0.501
LIG_UBA3_1	285	289	PF00899	0.358
MOD_CDC14_SPxK_1	411	414	PF00782	0.402
MOD_CDK_SPK_2	520	525	PF00069	0.731
MOD_CDK_SPxK_1	408	414	PF00069	0.409
MOD_CK1_1	126	132	PF00069	0.358
MOD_CK1_1	15	21	PF00069	0.774
MOD_CK1_1	156	162	PF00069	0.335
MOD_CK1_1	228	234	PF00069	0.356
MOD_CK1_1	245	251	PF00069	0.358
MOD_CK1_1	307	313	PF00069	0.326
MOD_CK1_1	5	11	PF00069	0.766
MOD_CK1_1	518	524	PF00069	0.597
MOD_CK1_1	536	542	PF00069	0.666
MOD_CK1_1	545	551	PF00069	0.646
MOD_CK1_1	568	574	PF00069	0.720
MOD_CK1_1	607	613	PF00069	0.724
MOD_CK1_1	616	622	PF00069	0.666
MOD_CK1_1	717	723	PF00069	0.660
MOD_CK1_1	81	87	PF00069	0.377
MOD_CK2_1	148	154	PF00069	0.396
MOD_CK2_1	158	164	PF00069	0.346
MOD_CK2_1	241	247	PF00069	0.293
MOD_CK2_1	271	277	PF00069	0.377
MOD_CK2_1	454	460	PF00069	0.552
MOD_CK2_1	532	538	PF00069	0.686
MOD_CK2_1	61	67	PF00069	0.506
MOD_CK2_1	639	645	PF00069	0.558
MOD_CK2_1	91	97	PF00069	0.335
MOD_GlcNHglycan	125	128	PF01048	0.358
MOD_GlcNHglycan	558	561	PF01048	0.724
MOD_GlcNHglycan	615	618	PF01048	0.673
MOD_GlcNHglycan	641	644	PF01048	0.572
MOD_GlcNHglycan	645	649	PF01048	0.632
MOD_GSK3_1	128	135	PF00069	0.442
MOD_GSK3_1	140	147	PF00069	0.377
MOD_GSK3_1	15	22	PF00069	0.740
MOD_GSK3_1	150	157	PF00069	0.288
MOD_GSK3_1	241	248	PF00069	0.519
MOD_GSK3_1	258	265	PF00069	0.393
MOD_GSK3_1	267	274	PF00069	0.412
MOD_GSK3_1	520	527	PF00069	0.651
MOD_GSK3_1	532	539	PF00069	0.621
MOD_GSK3_1	542	549	PF00069	0.636
MOD_GSK3_1	561	568	PF00069	0.723
MOD_GSK3_1	599	606	PF00069	0.769
MOD_GSK3_1	709	716	PF00069	0.564
MOD_N-GLC_1	281	286	PF02516	0.358
MOD_N-GLC_2	652	654	PF02516	0.611
MOD_NEK2_1	121	126	PF00069	0.316
MOD_NEK2_1	158	163	PF00069	0.341
MOD_NEK2_1	241	246	PF00069	0.408
MOD_NEK2_1	304	309	PF00069	0.329
MOD_NEK2_1	313	318	PF00069	0.305
MOD_NEK2_1	537	542	PF00069	0.633
MOD_NEK2_1	543	548	PF00069	0.597
MOD_NEK2_1	689	694	PF00069	0.534
MOD_NEK2_2	604	609	PF00069	0.563
MOD_NEK2_2	61	66	PF00069	0.529
MOD_PIKK_1	245	251	PF00454	0.438
MOD_PIKK_1	307	313	PF00454	0.326
MOD_PIKK_1	454	460	PF00454	0.476
MOD_PIKK_1	525	531	PF00454	0.585
MOD_PIKK_1	537	543	PF00454	0.802
MOD_PIKK_1	7	13	PF00454	0.647
MOD_PKA_2	12	18	PF00069	0.885
MOD_PKA_2	226	232	PF00069	0.364
MOD_PKA_2	251	257	PF00069	0.305
MOD_PKA_2	262	268	PF00069	0.305
MOD_PKA_2	304	310	PF00069	0.311
MOD_PKA_2	313	319	PF00069	0.358
MOD_PKA_2	454	460	PF00069	0.476
MOD_PKA_2	518	524	PF00069	0.728
MOD_PKA_2	568	574	PF00069	0.756
MOD_PKA_2	717	723	PF00069	0.679
MOD_Plk_1	153	159	PF00069	0.476
MOD_Plk_1	281	287	PF00069	0.358
MOD_Plk_1	336	342	PF00069	0.305
MOD_Plk_2-3	148	154	PF00069	0.335
MOD_Plk_4	128	134	PF00069	0.305
MOD_Plk_4	158	164	PF00069	0.370
MOD_Plk_4	28	34	PF00069	0.645
MOD_Plk_4	281	287	PF00069	0.358
MOD_Plk_4	313	319	PF00069	0.363
MOD_Plk_4	61	67	PF00069	0.469
MOD_Plk_4	616	622	PF00069	0.652
MOD_Plk_4	653	659	PF00069	0.577
MOD_ProDKin_1	19	25	PF00069	0.616
MOD_ProDKin_1	2	8	PF00069	0.796
MOD_ProDKin_1	26	32	PF00069	0.602
MOD_ProDKin_1	327	333	PF00069	0.358
MOD_ProDKin_1	408	414	PF00069	0.409
MOD_ProDKin_1	520	526	PF00069	0.621
MOD_ProDKin_1	608	614	PF00069	0.666
MOD_ProDKin_1	72	78	PF00069	0.358
MOD_SUMO_rev_2	176	183	PF00179	0.418
MOD_SUMO_rev_2	231	239	PF00179	0.385
MOD_SUMO_rev_2	265	274	PF00179	0.427
MOD_SUMO_rev_2	347	355	PF00179	0.335
MOD_SUMO_rev_2	402	411	PF00179	0.415
MOD_SUMO_rev_2	460	466	PF00179	0.501
TRG_DiLeu_BaLyEn_6	102	107	PF01217	0.305
TRG_ENDOCYTIC_2	171	174	PF00928	0.455
TRG_ENDOCYTIC_2	187	190	PF00928	0.410
TRG_ENDOCYTIC_2	85	88	PF00928	0.471
TRG_ER_diArg_1	297	300	PF00400	0.397
TRG_ER_diArg_1	36	39	PF00400	0.472
TRG_ER_diArg_1	633	636	PF00400	0.624
TRG_ER_diArg_1	704	706	PF00400	0.525
TRG_NES_CRM1_1	249	262	PF08389	0.335
TRG_NLS_MonoCore_2	37	42	PF00514	0.453
TRG_NLS_MonoExtC_3	36	41	PF00514	0.421
TRG_NLS_MonoExtC_3	501	507	PF00514	0.467
TRG_NLS_MonoExtN_4	37	42	PF00514	0.433
TRG_Pf-PMV_PEXEL_1	421	426	PF00026	0.411
TRG_Pf-PMV_PEXEL_1	450	454	PF00026	0.373

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P647	Leptomonas seymouri	68%	99%
A0A0S4IJP8	Bodo saltans	50%	97%
A0A1X0NNY0	Trypanosomatidae	65%	100%
A0A3S7WS99	Leishmania donovani	92%	100%
A0A3S7X9Y1	Leishmania donovani	31%	100%
A0A422NBP2	Trypanosoma rangeli	63%	100%
A4H720	Leishmania braziliensis	84%	100%
A4HND6	Leishmania braziliensis	30%	100%
A4HVE9	Leishmania infantum	92%	100%
A4IC09	Leishmania infantum	31%	100%
D0A6W1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	100%
E9AFU7	Leishmania major	31%	100%
E9B6Z9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
Q4QGE1	Leishmania major	92%	100%